Separation of red blood cells at high volume concentration under low centrifugal accelerations.

Abstract:

:The separation process of blood and RBC suspensions in a hematocrit range between 0.3-0.7 was investigated with a centrifuge allowed to run at low accelerations (100 xg-1000 xg). The position of the interface between the supernatant of plasma and the RBC column was continuously recorded by a new optoelectronic measuring system. The separation process could be mathematically described by an exponential decrease of the cell column approaching a final packing. At a given centrifugal acceleration the time constant is influenced by hematocrit, aggregation, deformation and plasma viscosity. The final packing depends linearly on the starting hematocrit (0.3-0.7) and can be used as a measure of deformability.

journal_name

Biorheology

journal_title

Biorheology

authors

Lerche D,Bilsing R

doi

10.3233/bir-1988-251-233

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

245-52

issue

1-2

eissn

0006-355X

issn

1878-5034

journal_volume

25

pub_type

杂志文章
  • Shear stress-induced binding of von Willebrand factor to platelets.

    abstract::Shear stress-induced platelet aggregation requires von Willebrand factor (vWF), platelet glycoprotein (GP) Ib, GPIIb-IIIa, Ca2+, and adenosine diphosphate (ADP). Recent reports using vWF labeled with either 125I or fluorescein isothiocyanate (FITC) have demonstrated that in shear-fields, vWF binds to both GPIb and GPI...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.1016/S0006-355X(97)00004-8

    authors: Konstantopoulos K,Chow TW,Turner NA,Hellums JD,Moake JL

    更新日期:1997-01-01 00:00:00

  • Mechanical assessment by magnetocytometry of the cytosolic and cortical cytoskeletal compartments in adherent epithelial cells.

    abstract::This study aims at quantifying the cellular mechanical properties based on a partitioning of the cytoskeleton in a cortical and a cytosolic compartments. The mechanical response of epithelial cells obtained by magnetocytometry - a micromanipulation technique which uses twisted ferromagnetic beads specifically linked t...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Laurent VM,Planus E,Fodil R,Isabey D

    更新日期:2003-01-01 00:00:00

  • Flow behaviour of a POSS biopolymer solution.

    abstract::A non-biodegradable polyhedral oligomeric silsesquioxane (POSS) nanocomposite biopolymer has been developed for fabrication of medical devices and for tissue engineering human organs. The polymer in solution, containing 2 wt% of POSS, has been synthesized, characterized and investigated to determine its key rheologica...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Kidane AG,Edirisinghe MJ,Bonhoeffer P,Seifalian AM

    更新日期:2007-01-01 00:00:00

  • Viscosity and order in erythrocyte membranes studied with nanosecond fluorometry.

    abstract::The viscosity and the order in the interior of human erythrocyte membranes were investigated by the fluorescence depolarization technique in the nanosecond region with 1,6-diphenyl-1,3,5-hexatriene (DPH). After pulsed excitation with a polarized light, the fluorescence anisotropy ratio of DPH in membranes rapidly decr...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1986-23504

    authors: Araiso T,Shindo Y,Arai T,Nitta J,Kikuchi Y,Kakiuchi Y,Koyama T

    更新日期:1986-01-01 00:00:00

  • The effects of margination and red cell augmented platelet diffusivity on platelet adhesion in complex flow.

    abstract::The effects of platelet margination and enhanced platelet diffusivity, as induced by red blood cells, on the adhesion of platelets, were investigated for a range of haematocrits, under 2D axi-symmetric flow, simulating previous in vitro experiments [Microvasc. Res. 17 (1979), 238-262]. The effect of margination was in...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Jordan A,David T,Homer-Vanniasinkam S,Graham A,Walker P

    更新日期:2004-01-01 00:00:00

  • Parallel-plate flow chamber for studies of 3D flow-endothelium interaction.

    abstract::Flow induced shear stress influences vascular cellular biology and pathophysiology in numerous ways. Previous in vitro studies on interactions between flow and endothelial cells using parallel-plate flow chambers involve two-dimensional flows, whereas flows in larger vessels are commonly three-dimensional. We have con...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Watkins NV,Caro CG,Wang W

    更新日期:2002-01-01 00:00:00

  • Blood soluble drag-reducing polymers prevent lethality from hemorrhagic shock in acute animal experiments.

    abstract::Over the past several decades, blood-soluble drag reducing polymers (DRPs) have been shown to significantly enhance hemodynamics in various animal models when added to blood at nanomolar concentrations. In the present study, the effects of the DRPs on blood circulation were tested in anesthetized rats exposed to acute...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Kameneva MV,Wu ZJ,Uraysh A,Repko B,Litwak KN,Billiar TR,Fink MP,Simmons RL,Griffith BP,Borovetz HS

    更新日期:2004-01-01 00:00:00

  • Effects of vessel size, cell sedimentation and haematocrit on the adhesion of leukocytes and platelets from flowing blood.

    abstract:BACKGROUND:Leukocytes and platelets typically fulfil their functions through adhesion to the walls of vessels with different size, haematocrit and shear rate. OBJECTIVE:We aimed to investigate differential effects of these variables on leukocyte and platelet adhesion. METHODS:Blood with varying haematocrit was perfus...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-15043

    authors: Watts T,Barigou M,Nash GB

    更新日期:2015-01-01 00:00:00

  • Erythrocytes sedimentation profiles under gravitational field as determined by He-Ne laser. VII. Influence of dextrans, albumin and saline on cellular aggregation and sedimentation rate.

    abstract::The erythrocytes sedimentation profiles (ESP) of normal blood and of blood mixed with saline, albumin (7%), and various molecular weight dextrans of different concentrations, at various height and widths of the sample holder are determined. These observations show that the sedimentation characteristics of the erythroc...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1987-24106

    authors: Singh M,Joseph KP

    更新日期:1987-01-01 00:00:00

  • Acute effects of continuous and intermittent aerobic exercises on hemorheological parameters: a pilot study.

    abstract:BACKGROUND:Acute hemorheological responses to different types of aerobic exercises have never been compared in a single study in healthy people. OBJECTIVE:We aimed to compare acute effects of high intensity intermittent exercise (HIIE) and moderate intensity continuous exercise (MICE) on hemorheological parameters, in...

    journal_title:Biorheology

    pub_type: 杂志文章,随机对照试验

    doi:10.3233/BIR-14012

    authors: Findikoglu G,Kilic-Toprak E,Kilic-Erkek O,Senol H,Bor-Kucukatay M

    更新日期:2014-01-01 00:00:00

  • Required test duration for group comparisons in ligament viscoelasticity: a statistical approach.

    abstract::The goal of this study was to determine the duration of time that ligaments from a study group need to be loaded in order to adequately determine their collective viscoelastic behavior. Rat ligaments were subjected either to creep or stress relaxation for 1,000 s or stress relaxation for 10,000 s to compare estimates ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Manley E Jr,Provenzano PP,Heisey D,Lakes R,Vanderby R Jr

    更新日期:2003-01-01 00:00:00

  • Predicted wall shear rate gradients in T-type arteriolar bifurcations.

    abstract::The purpose of this study was to examine the theoretical impact of the local bifurcation geometry on the shear rate gradient in a divergent arteriolar-type bifurcation. Newtonian flow through an arteriolar bifurcation was modeled using 3-dimensional computational fluid dynamics (CFD). Branching angles of 30 degrees, 5...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Noren D,Palmer HJ,Frame MD

    更新日期:2000-01-01 00:00:00

  • The role of mucus viscoelasticity in cough clearance.

    abstract::The relationships between mucus rheology, depth of mucus layer and clearance by simulated cough were examined in a study employing a model plexiglass trachea lined with gels formed from locust bean gum crosslinked with sodium tetraborate. The viscoelastic properties of the mucus simulants were determined by magnetic r...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1987-24611

    authors: King M

    更新日期:1987-01-01 00:00:00

  • Epinephrine and shear stress synergistically induce platelet aggregation via a mechanism that partially bypasses VWF-GP IB interactions.

    abstract::Elevated shear stress levels in pathologically stenosed vessels induce platelet activation and aggregation, and may play a role in the pathogenesis of arterial disease. Increased plasma catecholamine concentrations have also been implicated in the onset of acute coronary ischemic syndromes. This study was designed to ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.1016/0006-355X(96)00018-2

    authors: Wagner CT,Kroll MH,Chow TW,Hellums JD,Schafer AI

    更新日期:1996-05-01 00:00:00

  • Intensification of ciliary motility by extracellular ATP.

    abstract::Ciliary metachronism and motility were examined optically in tissue cultures from frog palate epithelium as a function of extracellular ATP concentration in the range of 10(-7)-10(-3) M. The main findings were: a) upon addition of ATP the metachronal wavelength increased by a factor of up to 2. b) the velocity of the ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1988-25309

    authors: Ovadyahu D,Eshel D,Priel Z

    更新日期:1988-01-01 00:00:00

  • Experimental assessment of wall shear flow in models.

    abstract::The blood flow immediately adjacent to the wall of a blood vessel or an artificial surface is of great interest. This flow defines the shear stress at the wall and is known to have a great physiological importance. The use of models is a viable method to investigate this flow. However, even in models the shear stress ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Affeld K,Kertzscher U,Goubergrits L

    更新日期:2002-01-01 00:00:00

  • The influence of cyclic tension amplitude on chondrocyte matrix synthesis: experimental and finite element analyses.

    abstract::While not generally viewed as physiologically significant in articular cartilage, substantial tension can develop in fibrocartilage structures and in articular cartilage injuries. This study examined how different amplitudes of cyclic tension influence chondrocyte matrix synthesis. Bovine articular chondrocytes seeded...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Connelly JT,Vanderploeg EJ,Levenston ME

    更新日期:2004-01-01 00:00:00

  • Characterisation and optimisation of foams for varicose vein sclerotherapy.

    abstract:BACKGROUND:Foam sclerotherapy is the process of using an aqueous foam to deliver surfactant to a varicose vein to damage vein wall endothelial cells, causing the vein to spasm, collapse and ultimately be re-absorbed into the body. Aqueous foams are complex fluids that can exhibit a significant yield stress and high eff...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-201004

    authors: Roberts TG,Cox SJ,Lewis AL,Jones SA

    更新日期:2021-01-13 00:00:00

  • Alterations in the biorheological features of some soft tissues after limb lengthening.

    abstract::The aim of this study was to investigate the effects of a surgical limb lengthening procedure on the biorheological features of some lengthened soft tissues. In this procedure external fixators were applied to goats' right radius to stretch the tissues. The right forelegs of goats were lengthened by 2, 4 cm, respectiv...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.1016/S0006-355X(99)80016-X

    authors: Huang K,Zeng Y,Xia H,Liu C

    更新日期:1998-07-01 00:00:00

  • Cross-sectional distributions of normal and abnormal red blood cells in capillary tubes determined by a new technique.

    abstract:BACKGROUND:In the microcirculation, red blood cells (RBCs) were observed to be confined to an axial stream surrounded by a marginal RBC depleted layer. This axial accumulation of RBCs is considered to arise from the RBC deformability. OBJECTIVE:To quantitatively evaluate the effect of RBC deformability on their axial ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-18166

    authors: Sasaki T,Seki J,Itano T,Sugihara-Seki M

    更新日期:2018-01-01 00:00:00

  • Platelet near-wall excess in porcine whole blood in artery-sized tubes under steady and pulsatile flow conditions.

    abstract::Platelet margination (enhanced platelet concentration in the near wall region of a blood vessel) has been well documented in small vessels. In artery-sized vessels margination has only been demonstrated in one study, using ghost cell suspensions and under relatively non-physiologic conditions of steady flow and 50 cm ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Xu C,Wootton DM

    更新日期:2004-01-01 00:00:00

  • Axial migration of spirulina microalgae in laminar tube flow.

    abstract::A dilute suspension of Spirulina Microalgae is found to exhibit radial migration in laminar flow in a 650 micron vertical tube. As the tube Reynolds number increases, the particles concentrate in a narrower region around the tube axis. When the turbulent regime is approached, the particles disperse as expected. ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1987-24626

    authors: Rakow AL,Chappell ML

    更新日期:1987-01-01 00:00:00

  • Increased monocyte actin polymerization in rat blood after intratracheal instillation of air pollution particles.

    abstract:BACKGROUND:Exposure to particulate air pollution is associated with an increased risk of cardiovascular disease. The mechanism by which exposure increases risk is poorly understood but could involve changes in the flow properties of blood. OBJECTIVE:The aim of this investigation was to assess the effect, in rats, of i...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-14027

    authors: Adams RA,Al-Mosawi A,Bérubé K,Jones T,Higgins T,Evans SA

    更新日期:2014-01-01 00:00:00

  • Assessment of oxidant susceptibility of red blood cells in various species based on cell deformability.

    abstract::The present study was designed to investigate the oxidant susceptibility of red blood cells (RBC) from four species (echidna, human, koala, Tasmanian devil) based on changes in cellular deformability. These species were specifically chosen based on differences in lifestyle and/or biology associated with varied levels ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-2012-0599

    authors: Simmonds MJ,Meiselman HJ,Marshall-Gradisnik SM,Pyne M,Kakanis M,Keane J,Brenu E,Christy R,Baskurt OK

    更新日期:2011-01-01 00:00:00

  • Effects of gender and age on thixotropic properties of whole blood from healthy adult subjects.

    abstract::The thixotropic parameters of whole blood from 314 healthy subjects (154 women, 160 men) were measured with our modified method by Low shear 30 Rheometer and calculated according Huang's equation. This communication offered the reference range of thixotropic parameters from man and woman group. The results demonstrate...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1991-283-409

    authors: Chen HQ,Zhong GH,Li L,Wang XY,Zhou T,Chen ZY

    更新日期:1991-01-01 00:00:00

  • Dynamic light scattering methods for biorhelogy.

    abstract::Several noninvasive light scattering techniques are reviewed that can be utilized in rheological studies of mucus and other soft biological matrices. Included are quasielastic light scattering techniques to determine the compressibility moduli of polymer lattices, resonance methods that can be used to measure the shea...

    journal_title:Biorheology

    pub_type: 杂志文章,评审

    doi:10.3233/bir-1987-24609

    authors: Nossal R

    更新日期:1987-01-01 00:00:00

  • Lubricated squeezing flow: a useful method for measuring the viscoelastic properties of soft tissues.

    abstract::Conducting experiments on very soft biological tissues can be difficult. Traditionally, unconfined compression and shear have been used. Here, an improved method of compression testing, lubricated squeezing flow is described. This gives a uniform compression along the squeezing axis and almost uniform equi-biaxial elo...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Nasseri S,Bilston L,Tanner R

    更新日期:2003-01-01 00:00:00

  • Influence of parenteral nutrition on blood rheology and platelet aggregation in vitro.

    abstract:BACKGROUND:Parenteral nutrition (PN) is a hyperosmolar solution composed of glucose, amino acids and a lipid emulsion, which is often used despite well-known side effects and complications. OBJECTIVES:In this study the hypothesis was tested that PN could affect hemorheology. METHODS:The influence of increasing plasma...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-140652

    authors: Compagnoni SC,Schulzki T,Thoeny S,Reinhart WH

    更新日期:2014-01-01 00:00:00

  • Ciliary activity under normal conditions and under viscous load.

    abstract::Ciliary metachronism and motility were examined optically in muco-ciliary tissue cultures from three different systems: a) frog's palate epithelium, b) frog's oesophagus, and c) human nasal polyps. In addition, lateral cilia of Mytilus edulis (water transporting cilia) were examined. It was revealed that the degree of...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1990-273-433

    authors: Gheber L,Priel Z

    更新日期:1990-01-01 00:00:00

  • Hemorheological factors of oxygen transfer in capillary tissue unit.

    abstract::The supply of oxygen to tissue from capillaries may be influenced by hemorheological factors. A hemorheological model is developed for elucidating which factors play a major role in the oxygen transport to tissue. For a capillary tissue unit, a modified cylindrical model of Krogh is used. For blood, two fluids model o...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1983-20517

    authors: Niimi H,Sugihara M,Yamakawa T

    更新日期:1983-01-01 00:00:00