Shear stress-induced binding of von Willebrand factor to platelets.

Abstract:

:Shear stress-induced platelet aggregation requires von Willebrand factor (vWF), platelet glycoprotein (GP) Ib, GPIIb-IIIa, Ca2+, and adenosine diphosphate (ADP). Recent reports using vWF labeled with either 125I or fluorescein isothiocyanate (FITC) have demonstrated that in shear-fields, vWF binds to both GPIb and GPIIb-IIIa. The sequence of the vWF finding to the two platelet receptors has not been precisely determined in these reports. In this study, a flow cytometry technique using a primary anti-vWF antibody and a secondary FITC IgG antibody was used to measure shear stress-induced vWF binding to platelets. Washed normal platelets suspended at 50,000/microliters with purified large vWF multimers were exposed to laminar shear stresses of 15 to 120 dynes/cm2 for 30 sec. At this low platelet count, little or no aggregation occurred in the shear fields. A significant increase in post-shear vWF-positive platelets was consistently observed. Experiments with platelets from normal and severe von Willebrand's disease (vWD) (which lack plasma and platelet alpha-granule vWF) demonstrated that exogenous vWF predominately contributed to the platelet-vWF binding. Blockade of platelet GPIb with the monoclonal anti-GPIb antibody, 6D1, completely inhibited shear stress-induced platelet-vWF attachment. In contrast, blockade of GPIIb-IIIa with monoclonal anti-GPIIb-IIIa antibodies, 10E5, or c7E3, or with the GPIIb-IIIa-blocking tetrapeptide, RGDS had little or no inhibitory effect on platelet-vWF binding. These data demonstrate that the binding of vWF to GPIb is likely to be the initial shear-induced platelet-ligand binding event.

journal_name

Biorheology

journal_title

Biorheology

authors

Konstantopoulos K,Chow TW,Turner NA,Hellums JD,Moake JL

doi

10.1016/S0006-355X(97)00004-8

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

57-71

issue

1

eissn

0006-355X

issn

1878-5034

pii

S0006-355X(97)00004-8

journal_volume

34

pub_type

杂志文章
  • An LDA and flow visualization study of pulsatile flow in an aortic bifurcation model.

    abstract::The structure of pulsatile flow in a rigid aortic bifurcation model was studied by means of a flow visualization technique and three-dimensional laser-Doppler anemometry. The model was made of glass, having the same shape as that of the average human aortic bifurcation. It was installed into a mock circulatory loop th...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1995-32104

    authors: Naiki T,Hayashi K,Takemura S

    更新日期:1995-01-01 00:00:00

  • Viscosity measurements of DNA solutions with and without condensing agents.

    abstract::Aqueous solutions calf thymus DNA at three concentrations were studied by viscosity measurements at ambient pressure. A gravitational capillary viscometer and a rotating cylinder viscometer were employed. Three polycations were tested as viscosity-reducing agents with measurements from 25°C to 50°C. The most significa...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-140649

    authors: Laesecke A,Burger JL

    更新日期:2014-01-01 00:00:00

  • A boundary layer model for wall shear stress in arterial stenosis.

    abstract::This paper proposes a model for wall shear stress in arterial stenosis based on boundary layer theory. Wall shear stress estimates are obtained by solving the momentum integral equation using the method proposed by Walz and applying this method to various stenosis geometries for Reynolds numbers (Re) of Re = 59-1000. ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Provenzano PP,Rutland CJ

    更新日期:2002-01-01 00:00:00

  • Optical twisting to monitor the rheology of single cells.

    abstract:BACKGROUND:Biological cells exhibit complex mechanical properties which determine their responses to applied force. OBJECTIVE:We developed an optical method to probe the temporal evolution of power-law rheology of single cells. METHODS:The method consisted in applying optically a constant mechanical torque to a biref...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-15084

    authors: de Saint Vincent MR

    更新日期:2016-05-26 00:00:00

  • The role of mucus viscoelasticity in cough clearance.

    abstract::The relationships between mucus rheology, depth of mucus layer and clearance by simulated cough were examined in a study employing a model plexiglass trachea lined with gels formed from locust bean gum crosslinked with sodium tetraborate. The viscoelastic properties of the mucus simulants were determined by magnetic r...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1987-24611

    authors: King M

    更新日期:1987-01-01 00:00:00

  • In vivo correlates of altered blood rheology.

    abstract::It is has been known for more than 80 years that compared to in vitro determinations, blood behaves as a less viscous fluid under in vivo flow conditions. The experiments of Whittaker and Winton were among the first dealing with the in vivo effects of altered blood rheology, and experimental studies during the second ...

    journal_title:Biorheology

    pub_type: 杂志文章,评审

    doi:

    authors: Baskurt OK

    更新日期:2008-01-01 00:00:00

  • A new view of mechanotransduction and strain amplification in cells with microvilli and cell processes.

    abstract::In this paper we shall describe new mechanical models for the deformation of the actin filament bundles in kidney microvilli and osteocytic cell processes to see whether these cellular extensions, like the stereocilia on hair cells in the inner ear, can function as mechanotransducers when subject to physiological flow...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Weinbaum S,Guo P,You L

    更新日期:2001-01-01 00:00:00

  • Mechanical assessment by magnetocytometry of the cytosolic and cortical cytoskeletal compartments in adherent epithelial cells.

    abstract::This study aims at quantifying the cellular mechanical properties based on a partitioning of the cytoskeleton in a cortical and a cytosolic compartments. The mechanical response of epithelial cells obtained by magnetocytometry - a micromanipulation technique which uses twisted ferromagnetic beads specifically linked t...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Laurent VM,Planus E,Fodil R,Isabey D

    更新日期:2003-01-01 00:00:00

  • Settling of fixed erythrocyte suspension droplets.

    abstract::The fractionation of micron-size particles according to physical properties of size, density and surface characteristics by centrifugation and electrophoresis is hindered when the particles behave collectively rather than individually. The formation and sedimentation of droplets containing particles is an extreme exam...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1983-20201

    authors: Omenyi SN,Snyder RS

    更新日期:1983-01-01 00:00:00

  • A macroscopic description of stress relaxation in a muscle subject to stepwise deformation.

    abstract::Stress relaxation regimes arising in a muscle subject to stepwise deformation are described on the basis of a recent phenomenological model of fully activated muscle tissue which is presented in the form of a second-order constitutive equation. It is shown that this model reproduces the qualitative form of relaxation ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1984-21606

    authors: Timanin EM

    更新日期:1984-01-01 00:00:00

  • Drag reducing polymers improve tissue perfusion via modification of the RBC traffic in microvessels.

    abstract::This paper reports a novel, physiologically significant, microfluidic phenomenon generated by nanomolar concentrations of drag-reducing polymers (DRP) dissolved in flowing blood, which may explain previously demonstrated beneficial effects of DRP on tissue perfusion. In microfluidic systems used in this study, DRP add...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-2009-0543

    authors: Marhefka JN,Zhao R,Wu ZJ,Velankar SS,Antaki JF,Kameneva MV

    更新日期:2009-01-01 00:00:00

  • Acute effects of continuous and intermittent aerobic exercises on hemorheological parameters: a pilot study.

    abstract:BACKGROUND:Acute hemorheological responses to different types of aerobic exercises have never been compared in a single study in healthy people. OBJECTIVE:We aimed to compare acute effects of high intensity intermittent exercise (HIIE) and moderate intensity continuous exercise (MICE) on hemorheological parameters, in...

    journal_title:Biorheology

    pub_type: 杂志文章,随机对照试验

    doi:10.3233/BIR-14012

    authors: Findikoglu G,Kilic-Toprak E,Kilic-Erkek O,Senol H,Bor-Kucukatay M

    更新日期:2014-01-01 00:00:00

  • Upregulation of aggrecan and type II collagen mRNA expression in bovine chondrocytes by the application of hydrostatic pressure.

    abstract::The aim of this study was to investigate the effect of hydrostatic pressure on the expression of messenger ribonucleic acid (mRNA) for specific extracellular matrix proteins in chondrocytes. Chondrocytes obtained from bovine metatarsophalangeal joints were embedded in cylindrical 2% agarose gels. A novel experimental ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Toyoda T,Seedhom BB,Kirkham J,Bonass WA

    更新日期:2003-01-01 00:00:00

  • Biorheological characteristics of skin after expansion.

    abstract::This study investigated the changes in biorheological characteristics of skin after expansion. Expanders were implanted into the back of eight adult dogs, and after an eight-week expansion the expanded and the non-expanded control skins were transferred to an adjacent site. The expanded specimens and their controls we...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Zeng Y,Huang K,Xu C,Zhang J,Sun G

    更新日期:2001-01-01 00:00:00

  • An inflammation-responsive transcription factor in the pathophysiology of osteoarthritis.

    abstract::A number of risk factors including biomechanical stress on the articular cartilage imposed by joint overloading due to obesity, repetitive damage of the joint tissues by injury of the menisci and ligaments, and abnormal joint alignment play a significant role in the onset of osteoarthritis (OA). Genetic predisposition...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Ray A,Ray BK

    更新日期:2008-01-01 00:00:00

  • Intravital-microscopic observations on the intravascular behavior of blood cell components during dietary-induced hyperlipidemia in the male rabbit.

    abstract::In normal male rabbits loaded dietary cholesterol, intravital-microscopy revealed a marked acceleration of intravascular adhesiveness of white blood cells and aggregability of red blood cells and a swarming of lipid-laden macrophages in connective tissue space concurrently with a systemic hyperlipidemia and anemia. Po...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1988-251-242

    authors: Asano M,Ohkubo C,Hirokawa A,Sasaki A,Tochihara Y,Chiba Y

    更新日期:1988-01-01 00:00:00

  • Segregation by size difference in binary suspensions of fluid droplets in channel flow.

    abstract::In channel flow of multicomponent suspensions, segregation behavior of suspended components perpendicular to the flow direction is often observed, which is considered to be caused by the differential properties of the lateral migration depending on their shape, size, flexibility, and other characteristics. In the pres...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-130638

    authors: Makino M,Sugihara-Seki M

    更新日期:2013-01-01 00:00:00

  • Limb bud mesenchyme cultured under tensile strain remodel collagen type I tubes to produce fibrillar collagen type II.

    abstract::In this work, we studied the effects of tensile strain on limb bud mesenchymal cells (MSC) cultured on a collagen type I tubular scaffold. A novel bioreactor was designed to culture the cells while subjecting the tubular scaffold to tensile stress and strain. Control samples included unseeded and MSC-seeded tubes cult...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-2009-0553

    authors: Amos JR,Li S,Yost M,Ploehn H,Potts JD

    更新日期:2009-01-01 00:00:00

  • Effect of nonaxisymmetric hematocrit distribution on non-Newtonian blood flow in small tubes.

    abstract::Hematocrit distribution and red blood cell aggregation are the major determinants of blood flow in narrow tubes at low flow rates. It has been observed experimentally that in microcirculation the hematocrit distribution is not uniform. This nonuniformity may result from plasma skimming and cell screening effects and a...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.1016/S0006-355X(98)00018-3

    authors: Das B,Johnson PC,Popel AS

    更新日期:1998-01-01 00:00:00

  • Morphodynamics of flow through sinuous curvatures of the heart.

    abstract::Through cardiac looping during embryonic development, human and other vertebrate hearts adopt sinuous curvatures with marked changes in direction of flow at atrial, ventricular and arterial levels. We used magnetic resonance phase velocity mapping to study flow through the hearts of resting volunteers, and Doppler ult...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Kilner PJ,Yang GZ,Firmin DN

    更新日期:2002-01-01 00:00:00

  • Lubricated squeezing flow: a useful method for measuring the viscoelastic properties of soft tissues.

    abstract::Conducting experiments on very soft biological tissues can be difficult. Traditionally, unconfined compression and shear have been used. Here, an improved method of compression testing, lubricated squeezing flow is described. This gives a uniform compression along the squeezing axis and almost uniform equi-biaxial elo...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Nasseri S,Bilston L,Tanner R

    更新日期:2003-01-01 00:00:00

  • Rheological properties and wall structures of large veins.

    abstract::The static and dynamic viscoelastic properties were studied of longitudinal and circumferential strips excised from various large veins of dogs. The mechanical behavior in longitudinal direction could be regarded as elastic, while that in circumferential direction was highly viscoelastic. No distinct regionality was o...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1983-20510

    authors: Hasegawa M

    更新日期:1983-01-01 00:00:00

  • Alterations in the biorheological features of some soft tissues after limb lengthening.

    abstract::The aim of this study was to investigate the effects of a surgical limb lengthening procedure on the biorheological features of some lengthened soft tissues. In this procedure external fixators were applied to goats' right radius to stretch the tissues. The right forelegs of goats were lengthened by 2, 4 cm, respectiv...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.1016/S0006-355X(99)80016-X

    authors: Huang K,Zeng Y,Xia H,Liu C

    更新日期:1998-07-01 00:00:00

  • Time-dependent rheological behaviour of blood flow at low shear in narrow horizontal tubes.

    abstract::Magnitude and time-dependence of the effects of red cell aggregation and sedimentation on the rheology of human blood were studied during low shear (tau W 2.5 to 92 mPa) flow through horizontal tubes (ID 25 to 105 microns). Immediately following reduction of perfusion pressure to a low value the red cell concentration...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1989-26210

    authors: Alonso C,Pries AR,Gaehtgens P

    更新日期:1989-01-01 00:00:00

  • Assessment of oxidant susceptibility of red blood cells in various species based on cell deformability.

    abstract::The present study was designed to investigate the oxidant susceptibility of red blood cells (RBC) from four species (echidna, human, koala, Tasmanian devil) based on changes in cellular deformability. These species were specifically chosen based on differences in lifestyle and/or biology associated with varied levels ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-2012-0599

    authors: Simmonds MJ,Meiselman HJ,Marshall-Gradisnik SM,Pyne M,Kakanis M,Keane J,Brenu E,Christy R,Baskurt OK

    更新日期:2011-01-01 00:00:00

  • Hemorheological effects of secoisolariciresinol in ovariectomized rats.

    abstract:BACKGROUND:Postmenopausal women often develop hemorheological disorders which may affect the systemic blood circulation and present a cardiovascular risk factor. OBJECTIVE:We evaluated effects of secoisolariciresinol (SECO), a phytoestrogen, on hemorheological parameters and lipid peroxidation in a model of the age-re...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-15066

    authors: Maslov MY,Plotnikova TM,Anishchenko AM,Aliev OI,Nifantiev NE,Plotnikov MB

    更新日期:2016-01-07 00:00:00

  • Molecular basis of red cell membrane rheology. Part 1.

    abstract::The biorheological properties and behavior of red blood cells (RBCs), as other types of cells, have a biochemical and molecular basis. The shape maintenance and deformability of RBCs depend on the structural and functional integrity of the membrane proteins. These proteins are composed of transmembrane proteins insert...

    journal_title:Biorheology

    pub_type: 杂志文章,评审

    doi:10.3233/bir-1990-273-410

    authors: Chien S,Sung LP

    更新日期:1990-01-01 00:00:00

  • Study of red cell aggregation in pulsatile flow from ultrasonic Doppler power measurements.

    abstract::Human red cell aggregability and disaggregability represent important hemorheological parameters of blood. Several techniques have been proposed to evaluate the tendency of red cells to form aggregates and to disrupt in the presence of shear stress. One of the most recent approaches is based on the characterization of...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1993-305-615

    authors: Cloutier G,Shung KK

    更新日期:1993-09-01 00:00:00

  • Cell-free layer and wall shear stress variation in microvessels.

    abstract::In this study, we simulated multiple red blood cells flowing through straight microvessels with the immersed-boundary lattice-Boltzmann model to examine the shear stress variation on the microvessel surface and its relation to the properties of cell-free layer. Significant variation in shear stress has been observed d...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-2012-0608

    authors: Yin X,Zhang J

    更新日期:2012-01-01 00:00:00

  • Characterisation and optimisation of foams for varicose vein sclerotherapy.

    abstract:BACKGROUND:Foam sclerotherapy is the process of using an aqueous foam to deliver surfactant to a varicose vein to damage vein wall endothelial cells, causing the vein to spasm, collapse and ultimately be re-absorbed into the body. Aqueous foams are complex fluids that can exhibit a significant yield stress and high eff...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-201004

    authors: Roberts TG,Cox SJ,Lewis AL,Jones SA

    更新日期:2021-01-13 00:00:00