Viscosity measurements of DNA solutions with and without condensing agents.

Abstract:

:Aqueous solutions calf thymus DNA at three concentrations were studied by viscosity measurements at ambient pressure. A gravitational capillary viscometer and a rotating cylinder viscometer were employed. Three polycations were tested as viscosity-reducing agents with measurements from 25°C to 50°C. The most significant viscosity reduction was achieved with spermine in a solution of DNA at 2 mg/ml concentration. Measurements of DNA solutions without agents from 10°C to 90°C revealed concentration-dependent viscosity maxima in the range from 50°C to 65°C. The associated increases in viscosity were as high as 400%. A two-liquids model for solutions of double- and single-stranded DNA was developed that closely represented the experimental data.

journal_name

Biorheology

journal_title

Biorheology

authors

Laesecke A,Burger JL

doi

10.3233/BIR-140649

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

15-28

issue

1

eissn

0006-355X

issn

1878-5034

pii

F3N87J812X184286

journal_volume

51

pub_type

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