Abstract:
:Articular cartilage is a tissue designed to withstand compression during joint movement and, in vivo, is subjected to a wide range of mechanical loading forces. Mechanosensitivity has been demonstrated to influence chondrocyte metabolism and cartilage homeostasis, but the mechanisms underlying mechanotransduction in these cells are poorly understood. In many cell types mechanical stimulation induces increases of the cytosolic Ca2+ concentration that propagates from cell to cell as an intercellular Ca2+ wave. Cell-to-cell communication through gap junctions underlies tissue co-ordination of metabolism and sensitivity to extracellular stimuli: gap junctional permeability to intracellular second messengers allows signal transduction pathways to be shared among several cells, ultimately resulting in co-ordinated tissue responses. Mechanically-induced Ca2+ signalling was investigated with digital fluorescence video imaging in primary cultures of rabbit articular chondrocytes. Mechanical stimulation of a single cell, obtained by briefly distorting the plasmamembrane with a micropipette, induced a wave of increased Ca2+ that was communicated to surrounding cells. Intercellular Ca2+ spreading was inhibited by 18 alpha-glycyrrhetinic acid, suggesting the involvement of gap junctions in signal propagation. The functional expression of gap junctions was assessed, in confluent chondrocyte cultures, by the intercellular transfer of Lucifer yellow dye in microinjection experiments while the expression of connexin 43 could be detected in Western blots. A series of pharmacological tools known to interfere with the cell calcium handling capacity were employed to investigate the mechanism of mechanically-induced Ca2+ signalling. In the absence of extracellular Ca2+ mechanical stimulation induced communicated Ca2+ waves similar to controls. Mechanical stress induced Ca2+ influx both in the stimulated chondrocyte but not in the adjacent cells, as assessed by the Mn2+ quenching technique. Cells treatment with thapsigargin and with the phospholipase C inhibitor U73122 blocked mechanically-induced signal propagation. These results provide evidence that in chondrocytes mechanical stimulation activates phospholipase C, thus leading to an increase of intracellular inositol 1,4,5-trisphosphate. The second messenger, by permeating gap junctions, stimulates intracellular Ca2+ release in neighbouring cells. Intercellular Ca2+ waves may provide a mechanism to co-ordinate tissue responses in cartilage physiology.
journal_name
Biorheologyjournal_title
Biorheologyauthors
D'Andrea P,Calabrese A,Capozzi I,Grandolfo M,Tonon R,Vittur Fsubject
Has Abstractpub_date
2000-01-01 00:00:00pages
75-83issue
1-2eissn
0006-355Xissn
1878-5034journal_volume
37pub_type
杂志文章相关文献
BIORHEOLOGY文献大全abstract::The relationships between mucus rheology, depth of mucus layer and clearance by simulated cough were examined in a study employing a model plexiglass trachea lined with gels formed from locust bean gum crosslinked with sodium tetraborate. The viscoelastic properties of the mucus simulants were determined by magnetic r...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1987-24611
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND:Acute hemorheological responses to different types of aerobic exercises have never been compared in a single study in healthy people. OBJECTIVE:We aimed to compare acute effects of high intensity intermittent exercise (HIIE) and moderate intensity continuous exercise (MICE) on hemorheological parameters, in...
journal_title:Biorheology
pub_type: 杂志文章,随机对照试验
doi:10.3233/BIR-14012
更新日期:2014-01-01 00:00:00
abstract::In normal male rabbits loaded dietary cholesterol, intravital-microscopy revealed a marked acceleration of intravascular adhesiveness of white blood cells and aggregability of red blood cells and a swarming of lipid-laden macrophages in connective tissue space concurrently with a systemic hyperlipidemia and anemia. Po...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1988-251-242
更新日期:1988-01-01 00:00:00
abstract::Flow-induced changes in the red cell microstructure of human blood are identified from mechanical and optical evidence. On initiation of steady flow, a new microstructure develops as the shear strain increases through unit strain. This structure is identified with the formation of layers of red cells that slide on pla...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1994-31206
更新日期:1994-03-01 00:00:00
abstract::The presented paper is based on the conclusions of the preceding paper (1). On the basis of a general theoretical approach to the mechanics of heterogeneous materials published earlier / (2) , (3) , (4) / macroscopic constitutive equation of trabecular bone is deduced from the description of its microstructure. For th...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1983-20607
更新日期:1983-01-01 00:00:00
abstract::The effects of platelet margination and enhanced platelet diffusivity, as induced by red blood cells, on the adhesion of platelets, were investigated for a range of haematocrits, under 2D axi-symmetric flow, simulating previous in vitro experiments [Microvasc. Res. 17 (1979), 238-262]. The effect of margination was in...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::The importance of biomechanical forces in regulating normal chondrocyte metabolism is well established and the mechanisms whereby mechanical forces are transduced into biochemical responses by chondrocytes are beginning to be understood. Previous studies have indicated that cyclical mechanical stimulation induces incr...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The compressive stiffness of an elastic material is traditionally characterized by its Young's modulus. Young's modulus of articular cartilage can be directly measured using unconfined compression geometry by assuming the cartilage to be homogeneous and isotropic. In isotropic materials, Young's modulus can also be de...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::In channel flow of multicomponent suspensions, segregation behavior of suspended components perpendicular to the flow direction is often observed, which is considered to be caused by the differential properties of the lateral migration depending on their shape, size, flexibility, and other characteristics. In the pres...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-130638
更新日期:2013-01-01 00:00:00
abstract::A rigidity index (RI) related to red blood cell deformability was measured by using the hemorheometre. The RI for 13 patients homozygous for sickle cell disease was 109 +/- 44 at 37 degrees C and at atmospheric pO2. The filtration time curve as a function of pO2 is biphasic for sickle cell suspensions. The pO2 at whic...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1989-26409
更新日期:1989-01-01 00:00:00
abstract::The Cell Transit Analyser (CTA) provides a means to rapidly measure the deformability of large numbers of individual cells. It combines many of the advantages of micropipette studies with the simplicity and speed of filtrometry methods by measuring the duration of each resistive pulse generated as a cell passes throug...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1992-292-301
更新日期:1992-03-01 00:00:00
abstract::Frozen, stored mucus has been extensively used for transport studies but there is no clear evidence of the influence that the temperature and length of time of storage may have on the results. We stored frog mucus samples at -20 and -80 degrees C and analysed them on days zero, 2, 10, 30 and 90. At each temperature, a...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::The purpose of this study was to examine the theoretical impact of the local bifurcation geometry on the shear rate gradient in a divergent arteriolar-type bifurcation. Newtonian flow through an arteriolar bifurcation was modeled using 3-dimensional computational fluid dynamics (CFD). Branching angles of 30 degrees, 5...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::The role of clustered of L-selectin receptors on the leukocyte surface is discussed in connection with the postulated velocity-dependent formation of selectin-ligand tether bonds to interpret the mechanism of leukocyte tethering to, and rolling along, the vascular endothelium. The distinct feature of this step-wise pr...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Pentoxifylline (PTX) has been reported to enhance the early accumulation of neutrophils at the site of Staphylococcus aureus subcutaneous infection in mice (1) and to stimulate in vitro PMN chemotaxis, particularly under dense agarose (2). Among the biochemical events contributing to chemotaxis are actin polymerizatio...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1990-273-421
更新日期:1990-01-01 00:00:00
abstract::Atherosclerosis is the most frequent cause of death and severe chronic disability in North America and Europe. The atherosclerosis-prone apolipoprotein E (apoE)-deficient mice contain the entire spectrum of lesions observed during atherogenesis. Significant remodelling of the artery occurs in atherosclerosis. The aim ...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::The separation process of blood and RBC suspensions in a hematocrit range between 0.3-0.7 was investigated with a centrifuge allowed to run at low accelerations (100 xg-1000 xg). The position of the interface between the supernatant of plasma and the RBC column was continuously recorded by a new optoelectronic measuri...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1988-251-233
更新日期:1988-01-01 00:00:00
abstract::Stress relaxation regimes arising in a muscle subject to stepwise deformation are described on the basis of a recent phenomenological model of fully activated muscle tissue which is presented in the form of a second-order constitutive equation. It is shown that this model reproduces the qualitative form of relaxation ...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1984-21606
更新日期:1984-01-01 00:00:00
abstract::Although most apparent in permanently misshapen irreversibly sickled erythrocytes (ISC), biochemical and structural alterations are present in the majority of sickle cell membranes. The relationship of membrane rigidity to cell shape and its dependence upon the internal hemoglobin cytosol are not clarified. We therefo...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::The viscosity and the order in the interior of human erythrocyte membranes were investigated by the fluorescence depolarization technique in the nanosecond region with 1,6-diphenyl-1,3,5-hexatriene (DPH). After pulsed excitation with a polarized light, the fluorescence anisotropy ratio of DPH in membranes rapidly decr...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1986-23504
更新日期:1986-01-01 00:00:00
abstract::The assessment of vessel patency can be substantially improved by serial microvessel diameter measurements taken successively along an extensive length of the vessel. It is possible to avoid making the a priori assumptions about the existence or location of local constriction sites implicit in single diameter measurem...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1991-283-420
更新日期:1991-01-01 00:00:00
abstract::During the recent years various investigators have developed the technique of kryophotometric measurements of oxyhemoglobin saturation with oxygen (psi) in small vessels. In this paper, on the basis of the statistical model of the organ developed by the authors, a theoretical relationship is established between the di...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1982-191-235
更新日期:1982-01-01 00:00:00
abstract::Platelet margination (enhanced platelet concentration in the near wall region of a blood vessel) has been well documented in small vessels. In artery-sized vessels margination has only been demonstrated in one study, using ghost cell suspensions and under relatively non-physiologic conditions of steady flow and 50 cm ...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::The supply of oxygen to tissue from capillaries may be influenced by hemorheological factors. A hemorheological model is developed for elucidating which factors play a major role in the oxygen transport to tissue. For a capillary tissue unit, a modified cylindrical model of Krogh is used. For blood, two fluids model o...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1983-20517
更新日期:1983-01-01 00:00:00
abstract::The electrical properties of red blood cell (RBC) suspensions are influenced by flow conditions, and prior studies indicate that electrical properties may reflect the kinetics of RBC aggregation. Changes of conductance and capacitance were monitored and had a time course resembling a "syllectogram" (i.e., temporal cha...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-2009-0541
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Parenteral nutrition (PN) is a hyperosmolar solution composed of glucose, amino acids and a lipid emulsion, which is often used despite well-known side effects and complications. OBJECTIVES:In this study the hypothesis was tested that PN could affect hemorheology. METHODS:The influence of increasing plasma...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-140652
更新日期:2014-01-01 00:00:00
abstract::Arterial hemodynamic forces may play a role in the localization of early atherosclerotic lesions. We have been developing numerical techniques based on overset or "Chimera" type formulations to solve the Navier-Stokes equations in complex geometries simulating arterial bifurcations. This paper presents three-dimension...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.1016/s0006-355x(99)80020-1
更新日期:1998-11-01 00:00:00
abstract:BACKGROUND:Postmenopausal women often develop hemorheological disorders which may affect the systemic blood circulation and present a cardiovascular risk factor. OBJECTIVE:We evaluated effects of secoisolariciresinol (SECO), a phytoestrogen, on hemorheological parameters and lipid peroxidation in a model of the age-re...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-15066
更新日期:2016-01-07 00:00:00
abstract::This study aims at quantifying the cellular mechanical properties based on a partitioning of the cytoskeleton in a cortical and a cytosolic compartments. The mechanical response of epithelial cells obtained by magnetocytometry - a micromanipulation technique which uses twisted ferromagnetic beads specifically linked t...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::While not generally viewed as physiologically significant in articular cartilage, substantial tension can develop in fibrocartilage structures and in articular cartilage injuries. This study examined how different amplitudes of cyclic tension influence chondrocyte matrix synthesis. Bovine articular chondrocytes seeded...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00