Abstract:
:The membrane shear elastic modulus (mu) and the time constant for extensional shape recovery (tc) were measured for normal, control human red blood cells (RBC) and for RBC heat treated (HT) at 48 degrees C. Three separate methods for the measurement of mu were compared (two used a micropipette and one employed a flow channel), and the membrane viscosity (n) was calculated from the relation n = mu. tc. The deformability of HT and control cells was evaluated using micropipette techniques, and the bulk viscosity of RBC suspensions at 40% hematocrit was measured. The shear elastic modulus, or "membrane rigidity", was more than doubled by heat treatment, although both the absolute value for mu and the estimate of the increase induced by heat treatment varied depending on the method of measurement. Heat treatment caused smaller increases in membrane viscosity and in membrane bending resistance, and only minimal changes in cell geometry. The deformability of HT cells was reduced: 1) the pressure required for cell entry (Pe) into 3 micrometers pipettes was increased, on average, by 170%; 2) at an aspiration pressure (Pa) exceeding Pe, longer times were required for cell entry into the same pipettes. However, when Pa was scaled relative to the mean entry pressure for a given sample (i.e, Pa/Pe), entry times were similar for control and HT cells. Bulk viscosity of HT RBC suspensions was elevated by approximately 12% on average (shear rates 75 to 1500 inverse seconds). These findings suggest that alteration of RBC membrane mechanical properties, similar to those induced by heat treatment, would most affect the in vivo circulation in regions where vessel dimensions are smaller than cellular diameters.
journal_name
Biorheologyjournal_title
Biorheologyauthors
Nash GB,Meiselman HJdoi
10.3233/bir-1985-22106subject
Has Abstractpub_date
1985-01-01 00:00:00pages
73-84issue
1eissn
0006-355Xissn
1878-5034journal_volume
22pub_type
杂志文章相关文献
BIORHEOLOGY文献大全abstract::It is has been known for more than 80 years that compared to in vitro determinations, blood behaves as a less viscous fluid under in vivo flow conditions. The experiments of Whittaker and Winton were among the first dealing with the in vivo effects of altered blood rheology, and experimental studies during the second ...
journal_title:Biorheology
pub_type: 杂志文章,评审
doi:
更新日期:2008-01-01 00:00:00
abstract::Stress relaxation regimes arising in a muscle subject to stepwise deformation are described on the basis of a recent phenomenological model of fully activated muscle tissue which is presented in the form of a second-order constitutive equation. It is shown that this model reproduces the qualitative form of relaxation ...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1984-21606
更新日期:1984-01-01 00:00:00
abstract::The goal of this work is to better understand the association between renal artery aneurysms and secondary hypertension through modeling the blood flow and the mechanics of diseased arteries. A large number of patients with renal artery aneurysms exhibit hypertension. Following surgical intervention, some patients exp...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-130623
更新日期:2013-01-01 00:00:00
abstract::The fractionation of micron-size particles according to physical properties of size, density and surface characteristics by centrifugation and electrophoresis is hindered when the particles behave collectively rather than individually. The formation and sedimentation of droplets containing particles is an extreme exam...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1983-20201
更新日期:1983-01-01 00:00:00
abstract::The maintenance of articular cartilage integrity requires a balance between anabolic and catabolic processes which are under the control of chondrocytes. These cells are living in an anaerobic environment and normally do not divide. They are responsible for the continuous maintenance of the cartilage extracellular mat...
journal_title:Biorheology
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::Aqueous solutions calf thymus DNA at three concentrations were studied by viscosity measurements at ambient pressure. A gravitational capillary viscometer and a rotating cylinder viscometer were employed. Three polycations were tested as viscosity-reducing agents with measurements from 25°C to 50°C. The most significa...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-140649
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Parenteral nutrition (PN) is a hyperosmolar solution composed of glucose, amino acids and a lipid emulsion, which is often used despite well-known side effects and complications. OBJECTIVES:In this study the hypothesis was tested that PN could affect hemorheology. METHODS:The influence of increasing plasma...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-140652
更新日期:2014-01-01 00:00:00
abstract::Flow-induced changes in the red cell microstructure of human blood are identified from mechanical and optical evidence. On initiation of steady flow, a new microstructure develops as the shear strain increases through unit strain. This structure is identified with the formation of layers of red cells that slide on pla...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1994-31206
更新日期:1994-03-01 00:00:00
abstract:BACKGROUND:RRx-001 is an anti-cancer immunotherapeutic that increases the sensitivity of drug resistant tumors via multiple mechanisms which involve binding to hemoglobin and enhancing nitrite reductase activity of deoxyhemoglobin. OBJECTIVE:In the present study, the effect of clinically used doses of RRx-001 on eryth...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-190213
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Exposure to particulate air pollution is associated with an increased risk of cardiovascular disease. The mechanism by which exposure increases risk is poorly understood but could involve changes in the flow properties of blood. OBJECTIVE:The aim of this investigation was to assess the effect, in rats, of i...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-14027
更新日期:2014-01-01 00:00:00
abstract::Through cardiac looping during embryonic development, human and other vertebrate hearts adopt sinuous curvatures with marked changes in direction of flow at atrial, ventricular and arterial levels. We used magnetic resonance phase velocity mapping to study flow through the hearts of resting volunteers, and Doppler ult...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Conducting experiments on very soft biological tissues can be difficult. Traditionally, unconfined compression and shear have been used. Here, an improved method of compression testing, lubricated squeezing flow is described. This gives a uniform compression along the squeezing axis and almost uniform equi-biaxial elo...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::The relationships between mucus rheology, depth of mucus layer and clearance by simulated cough were examined in a study employing a model plexiglass trachea lined with gels formed from locust bean gum crosslinked with sodium tetraborate. The viscoelastic properties of the mucus simulants were determined by magnetic r...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1987-24611
更新日期:1987-01-01 00:00:00
abstract::The purpose of this study was to determine the shear rate at the wall in a symmetrically branched tube with a branch-to-trunk area ratio and angle of branching that were comparable to the human abdominal aorta. Velocity profiles were measured with a laser Doppler anemometer during steady and pulsatile flow in which th...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1982-191-233
更新日期:1982-01-01 00:00:00
abstract::The electrical properties of red blood cell (RBC) suspensions are influenced by flow conditions, and prior studies indicate that electrical properties may reflect the kinetics of RBC aggregation. Changes of conductance and capacitance were monitored and had a time course resembling a "syllectogram" (i.e., temporal cha...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-2009-0541
更新日期:2009-01-01 00:00:00
abstract::Aggregation of human red blood cells (RBCs) induced by dextrans of various molecular weight has been studied by using a new ultrasonic interferometry method. This method, based on A-mode echography, allowed for the measurement of the accumulation rate of particles on a solid plate which is related to their sedimentati...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1991-281-209
更新日期:1991-01-01 00:00:00
abstract::Plasma fractionation by membrane filtration permits the reinfusion of the patient with his own albumin. In this study, the influence of membrane nature and plasma flux on plasma fractionation in dead-end mode is investigated with acetate hollow fiber filters. It is found that transmembrane pressure TMP rises exponenti...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1991-281-204
更新日期:1991-01-01 00:00:00
abstract::A number of risk factors including biomechanical stress on the articular cartilage imposed by joint overloading due to obesity, repetitive damage of the joint tissues by injury of the menisci and ligaments, and abnormal joint alignment play a significant role in the onset of osteoarthritis (OA). Genetic predisposition...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The purpose of the present study was to investigate the intracellular IL-1beta production and beta1 integrins (alpha4/beta1 and alpha5/beta1) expression on chondrocytes. Chondroytes monolayer (human chondrosarcoma cell line HEM-C55) were incubated for 12, 24 and 48 hours in the presence of Tumor Necrosis Factor-alpha ...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Frozen, stored mucus has been extensively used for transport studies but there is no clear evidence of the influence that the temperature and length of time of storage may have on the results. We stored frog mucus samples at -20 and -80 degrees C and analysed them on days zero, 2, 10, 30 and 90. At each temperature, a...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Using a large scale model and the concept of dimensional similarity, we have developed a dimensionless correlation which permits the estimation of the drag force of blood flow in an in vivo vessel on a leukocyte adherent to the vessel wall. This relationship is [formula: see text] where Re is a Reynolds number, Cd is ...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.1016/0006-355X(96)00011-X
更新日期:1996-03-01 00:00:00
abstract::This study investigated the changes in biorheological characteristics of skin after expansion. Expanders were implanted into the back of eight adult dogs, and after an eight-week expansion the expanded and the non-expanded control skins were transferred to an adjacent site. The expanded specimens and their controls we...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::The thixotropic parameters of whole blood from 314 healthy subjects (154 women, 160 men) were measured with our modified method by Low shear 30 Rheometer and calculated according Huang's equation. This communication offered the reference range of thixotropic parameters from man and woman group. The results demonstrate...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1991-283-409
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Postmenopausal women often develop hemorheological disorders which may affect the systemic blood circulation and present a cardiovascular risk factor. OBJECTIVE:We evaluated effects of secoisolariciresinol (SECO), a phytoestrogen, on hemorheological parameters and lipid peroxidation in a model of the age-re...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-15066
更新日期:2016-01-07 00:00:00
abstract:BACKGROUND:The contribution of proteoglycan 4 (PRG4) to synovial fluid and hyaluronan (HA) solution rheology are poorly understood. The effects of PRG4 disulfide-bonded structure on viscosity and viscosity of newly available full-length recombinant human PRG4 (rhPRG4) have not previously been reported. OBJECTIVE:This ...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-14037
更新日期:2014-01-01 00:00:00
abstract::Magnitude and time-dependence of the effects of red cell aggregation and sedimentation on the rheology of human blood were studied during low shear (tau W 2.5 to 92 mPa) flow through horizontal tubes (ID 25 to 105 microns). Immediately following reduction of perfusion pressure to a low value the red cell concentration...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1989-26210
更新日期:1989-01-01 00:00:00
abstract::Several noninvasive light scattering techniques are reviewed that can be utilized in rheological studies of mucus and other soft biological matrices. Included are quasielastic light scattering techniques to determine the compressibility moduli of polymer lattices, resonance methods that can be used to measure the shea...
journal_title:Biorheology
pub_type: 杂志文章,评审
doi:10.3233/bir-1987-24609
更新日期:1987-01-01 00:00:00
abstract::The supply of oxygen to tissue from capillaries may be influenced by hemorheological factors. A hemorheological model is developed for elucidating which factors play a major role in the oxygen transport to tissue. For a capillary tissue unit, a modified cylindrical model of Krogh is used. For blood, two fluids model o...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1983-20517
更新日期:1983-01-01 00:00:00
abstract::The effects of platelet margination and enhanced platelet diffusivity, as induced by red blood cells, on the adhesion of platelets, were investigated for a range of haematocrits, under 2D axi-symmetric flow, simulating previous in vitro experiments [Microvasc. Res. 17 (1979), 238-262]. The effect of margination was in...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::The aim of this study was to investigate the effects of a surgical limb lengthening procedure on the biorheological features of some lengthened soft tissues. In this procedure external fixators were applied to goats' right radius to stretch the tissues. The right forelegs of goats were lengthened by 2, 4 cm, respectiv...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.1016/S0006-355X(99)80016-X
更新日期:1998-07-01 00:00:00