In vitro study of intracellular IL-1beta production and beta1 integrins expression in stimulated chondrocytes--effect of rhein.

Abstract:

:The purpose of the present study was to investigate the intracellular IL-1beta production and beta1 integrins (alpha4/beta1 and alpha5/beta1) expression on chondrocytes. Chondroytes monolayer (human chondrosarcoma cell line HEM-C55) were incubated for 12, 24 and 48 hours in the presence of Tumor Necrosis Factor-alpha (TNF-alpha, Sigma, France) or recombinant human IL-1alpha (rh-IL1alpha, Becton Dickinson, France). After direct immunolabelling, cells were either analyzed on FACScan flow cytometer (Becton Dickinson, France), or observed under an epi-fluorescence inverted microscope equipped with the CellScan EPR optical scanning acquisition system (IPLab-Scanalytics, USA). We found that the IL-1beta mean fluorescence intensity in flow cytometry and in 3D microscopy was increased in the presence of TNF-alpha or rh-IL-1alpha, and alpha4/beta1 or alpha5/beta1 expression was higher on stimulated cells than on control cells. On the other hand, we have evaluated the in vitro effects of rhein (10(-5) M, Negma, France), an active metabolite of diacerein, on the intracellular IL-1beta and beta1 integrins expressed by stimulated or no-stimulated chondrocytes. The results indicated that rhein leads to a reduction of IL-1beta synthesis whereas a weak decrease of beta1 integrins receptors expression is observed. From this study, it seems that rhein partially reduce cytokine-induced intracellular IL-1beta production, and it has a weak action on alpha4/beta1 or alpha5/beta1 receptors.

journal_name

Biorheology

journal_title

Biorheology

authors

Gigant-Huselstein C,Dumas D,Payan E,Muller S,Bensoussan D,Netter P,Stoltz JF

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

277-85

issue

1-2

eissn

0006-355X

issn

1878-5034

journal_volume

39

pub_type

杂志文章
  • Non-Newtonian viscosity of human blood: flow-induced changes in microstructure.

    abstract::Flow-induced changes in the red cell microstructure of human blood are identified from mechanical and optical evidence. On initiation of steady flow, a new microstructure develops as the shear strain increases through unit strain. This structure is identified with the formation of layers of red cells that slide on pla...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1994-31206

    authors: Thurston GB

    更新日期:1994-03-01 00:00:00

  • Fluid shear stress enhanced DNA synthesis in cultured endothelial cells during repair of mechanical denudation.

    abstract::We have previously observed a stimulatory effect of fluid shear stress on the regeneration of cultured endothelial cell layers after mechanical denudation. In this study we examined how fluid shear stress affects endothelial cell DNA synthesis during regeneration. Following mechanical denudation of narrow linear areas...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1990-27505

    authors: Ando J,Komatsuda T,Ishikawa C,Kamiya A

    更新日期:1990-01-01 00:00:00

  • An inflammation-responsive transcription factor in the pathophysiology of osteoarthritis.

    abstract::A number of risk factors including biomechanical stress on the articular cartilage imposed by joint overloading due to obesity, repetitive damage of the joint tissues by injury of the menisci and ligaments, and abnormal joint alignment play a significant role in the onset of osteoarthritis (OA). Genetic predisposition...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Ray A,Ray BK

    更新日期:2008-01-01 00:00:00

  • In vivo correlates of altered blood rheology.

    abstract::It is has been known for more than 80 years that compared to in vitro determinations, blood behaves as a less viscous fluid under in vivo flow conditions. The experiments of Whittaker and Winton were among the first dealing with the in vivo effects of altered blood rheology, and experimental studies during the second ...

    journal_title:Biorheology

    pub_type: 杂志文章,评审

    doi:

    authors: Baskurt OK

    更新日期:2008-01-01 00:00:00

  • Computational study of the effect of geometric and flow parameters on the steady flow field at the rabbit aorto-celiac bifurcation.

    abstract::Arterial hemodynamic forces may play a role in the localization of early atherosclerotic lesions. We have been developing numerical techniques based on overset or "Chimera" type formulations to solve the Navier-Stokes equations in complex geometries simulating arterial bifurcations. This paper presents three-dimension...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.1016/s0006-355x(99)80020-1

    authors: Cheer AY,Dwyer HA,Barakat AI,Sy E,Bice M

    更新日期:1998-11-01 00:00:00

  • Performance characteristics of a model secreting acinus.

    abstract::An acinus is the principal secretion unit of an exocrine gland. This paper sets up a mathematical model of an acinus and examines the effect on the output of varying the operating parameters, both of the secretion mechanism and the fluid properties. Particular reference is made to acini of the pancreas. The question o...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1986-23103

    authors: Grigg CH,Fitz-Gerald JM

    更新日期:1986-01-01 00:00:00

  • The shear rate at the wall in a symmetrically branched tube simulating the aortic bifurcation.

    abstract::The purpose of this study was to determine the shear rate at the wall in a symmetrically branched tube with a branch-to-trunk area ratio and angle of branching that were comparable to the human abdominal aorta. Velocity profiles were measured with a laser Doppler anemometer during steady and pulsatile flow in which th...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1982-191-233

    authors: Walburn FJ,Stein PD

    更新日期:1982-01-01 00:00:00

  • Blood soluble drag-reducing polymers prevent lethality from hemorrhagic shock in acute animal experiments.

    abstract::Over the past several decades, blood-soluble drag reducing polymers (DRPs) have been shown to significantly enhance hemodynamics in various animal models when added to blood at nanomolar concentrations. In the present study, the effects of the DRPs on blood circulation were tested in anesthetized rats exposed to acute...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Kameneva MV,Wu ZJ,Uraysh A,Repko B,Litwak KN,Billiar TR,Fink MP,Simmons RL,Griffith BP,Borovetz HS

    更新日期:2004-01-01 00:00:00

  • Segregation by size difference in binary suspensions of fluid droplets in channel flow.

    abstract::In channel flow of multicomponent suspensions, segregation behavior of suspended components perpendicular to the flow direction is often observed, which is considered to be caused by the differential properties of the lateral migration depending on their shape, size, flexibility, and other characteristics. In the pres...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-130638

    authors: Makino M,Sugihara-Seki M

    更新日期:2013-01-01 00:00:00

  • Effects of vessel size, cell sedimentation and haematocrit on the adhesion of leukocytes and platelets from flowing blood.

    abstract:BACKGROUND:Leukocytes and platelets typically fulfil their functions through adhesion to the walls of vessels with different size, haematocrit and shear rate. OBJECTIVE:We aimed to investigate differential effects of these variables on leukocyte and platelet adhesion. METHODS:Blood with varying haematocrit was perfus...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-15043

    authors: Watts T,Barigou M,Nash GB

    更新日期:2015-01-01 00:00:00

  • Effect of couple stresses on the unsteady convective diffusion in fluid flow through a channel.

    abstract::A generalized dispersion model is used to obtain exact solution for unsteady convective diffusion in the presence of couple stresses. The effect of the couple stress parameter 'a' on the most dominant dispersion coefficient is clearly depicted. The dimensionless mean concentration distribution is obtained as a functio...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1986-23404

    authors: Rudraiah N,Pal D,Siddheshwar PG

    更新日期:1986-01-01 00:00:00

  • Assessment of red blood cell aggregation with dextran by ultrasonic interferometry.

    abstract::Aggregation of human red blood cells (RBCs) induced by dextrans of various molecular weight has been studied by using a new ultrasonic interferometry method. This method, based on A-mode echography, allowed for the measurement of the accumulation rate of particles on a solid plate which is related to their sedimentati...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1991-281-209

    authors: Razavian SM,Guillemin MT,Guillet R,Beuzard Y,Boynard M

    更新日期:1991-01-01 00:00:00

  • COX-1, COX-2 and articular joint disease: a role for chondroprotective agents.

    abstract::It is widely accepted that whilst exhibiting clinically useful anti-inflammatory and analgesic activity, the application of non-steroidal anti-inflammatory drugs (NSAIDs) does not affect the underlying pathogenesis of articular diseases such as rheumatoid arthritis. The demonstration of a role for COX-2 in the resolut...

    journal_title:Biorheology

    pub_type: 杂志文章,评审

    doi:

    authors: Colville-Nash PR,Willoughby DA

    更新日期:2002-01-01 00:00:00

  • Intercellular Ca2+ waves in mechanically stimulated articular chondrocytes.

    abstract::Articular cartilage is a tissue designed to withstand compression during joint movement and, in vivo, is subjected to a wide range of mechanical loading forces. Mechanosensitivity has been demonstrated to influence chondrocyte metabolism and cartilage homeostasis, but the mechanisms underlying mechanotransduction in t...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: D'Andrea P,Calabrese A,Capozzi I,Grandolfo M,Tonon R,Vittur F

    更新日期:2000-01-01 00:00:00

  • Plasma fractionation by dead-end membrane filtration: effects of membrane properties and plasma flux.

    abstract::Plasma fractionation by membrane filtration permits the reinfusion of the patient with his own albumin. In this study, the influence of membrane nature and plasma flux on plasma fractionation in dead-end mode is investigated with acetate hollow fiber filters. It is found that transmembrane pressure TMP rises exponenti...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1991-281-204

    authors: Charcosset C,Ding LH,Jaffrin MY

    更新日期:1991-01-01 00:00:00

  • A new view of mechanotransduction and strain amplification in cells with microvilli and cell processes.

    abstract::In this paper we shall describe new mechanical models for the deformation of the actin filament bundles in kidney microvilli and osteocytic cell processes to see whether these cellular extensions, like the stereocilia on hair cells in the inner ear, can function as mechanotransducers when subject to physiological flow...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Weinbaum S,Guo P,You L

    更新日期:2001-01-01 00:00:00

  • Mechanical regulation of proteoglycan synthesis in normal and osteoarthritic human articular chondrocytes--roles for alpha5 and alphaVbeta5 integrins.

    abstract::The importance of biomechanical forces in regulating normal chondrocyte metabolism is well established and the mechanisms whereby mechanical forces are transduced into biochemical responses by chondrocytes are beginning to be understood. Previous studies have indicated that cyclical mechanical stimulation induces incr...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Holledge MM,Millward-Sadler SJ,Nuki G,Salter DM

    更新日期:2008-01-01 00:00:00

  • Separation of red blood cells at high volume concentration under low centrifugal accelerations.

    abstract::The separation process of blood and RBC suspensions in a hematocrit range between 0.3-0.7 was investigated with a centrifuge allowed to run at low accelerations (100 xg-1000 xg). The position of the interface between the supernatant of plasma and the RBC column was continuously recorded by a new optoelectronic measuri...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1988-251-233

    authors: Lerche D,Bilsing R

    更新日期:1988-01-01 00:00:00

  • About the importance of account of some microcirculation parameters dispersion in modelling oxygen transport.

    abstract::During the recent years various investigators have developed the technique of kryophotometric measurements of oxyhemoglobin saturation with oxygen (psi) in small vessels. In this paper, on the basis of the statistical model of the organ developed by the authors, a theoretical relationship is established between the di...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1982-191-235

    authors: Sharikov AN,Zaiko VM

    更新日期:1982-01-01 00:00:00

  • Required test duration for group comparisons in ligament viscoelasticity: a statistical approach.

    abstract::The goal of this study was to determine the duration of time that ligaments from a study group need to be loaded in order to adequately determine their collective viscoelastic behavior. Rat ligaments were subjected either to creep or stress relaxation for 1,000 s or stress relaxation for 10,000 s to compare estimates ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Manley E Jr,Provenzano PP,Heisey D,Lakes R,Vanderby R Jr

    更新日期:2003-01-01 00:00:00

  • Size determination of red blood cell aggregates induced by dextran using ultrasound backscattering phenomenon.

    abstract::Different methods are commonly used to study the red blood cell aggregation phenomenon. The major interest of the ultrasonic method presently discussed is to assess the mean size of red blood cell (RBC) aggregates by measuring ultrasonic intensity backscattered by blood. Applying Rayleigh theory of sound to blood medi...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1990-27104

    authors: Boynard M,Lelievre JC

    更新日期:1990-01-01 00:00:00

  • Hyaluronan injection affects neither osteoarthritis progression nor loading of the OA knee in dogs.

    abstract::We previously reported that intraarticular injections of hyaluronan (HA), administered prophylactically to dogs in whom knee osteoarthritis had been induced by transection of the anterior cruicate ligament, did not significantly modify the intraarticular pathology but decreased the proteogylcan concentration of the ar...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Brandt KD,Smith GN,Myers SL

    更新日期:2004-01-01 00:00:00

  • Biorheology and fluid flux in swelling tissues. I. Bicomponent theory for small deformations, including concentration effects.

    abstract::A theory for the rheological behavior and fluid flux in swelling tissues under small deformations is presented. Tissues are considered as bicomponent solid-fluid mixtures. Concentration effects are included. The driving forces (body, surface and interactive), are discussed and their constitutive relationships to the t...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1987-24210

    authors: Lanir Y

    更新日期:1987-01-01 00:00:00

  • Acute effects of continuous and intermittent aerobic exercises on hemorheological parameters: a pilot study.

    abstract:BACKGROUND:Acute hemorheological responses to different types of aerobic exercises have never been compared in a single study in healthy people. OBJECTIVE:We aimed to compare acute effects of high intensity intermittent exercise (HIIE) and moderate intensity continuous exercise (MICE) on hemorheological parameters, in...

    journal_title:Biorheology

    pub_type: 杂志文章,随机对照试验

    doi:10.3233/BIR-14012

    authors: Findikoglu G,Kilic-Toprak E,Kilic-Erkek O,Senol H,Bor-Kucukatay M

    更新日期:2014-01-01 00:00:00

  • The role of aggregation kinetics in the sedimentation of erythtocytes.

    abstract::The well-known S-shaped settling curves are obtained as solutions of an autonomic dynamical system deduced mathematically from the generalized Stokes formula, the blood volume conservation law, and the Smoluchowski theory of particle coagulation. Numerical computations and parametric analysis of the deduced two nonlin...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.1016/0006-355x(96)00027-3

    authors: Petrov VG,Edissonov I

    更新日期:1996-07-01 00:00:00

  • Swirling flow can suppress monocyte adhesion in the flow disturbance zones of the endovascular stent.

    abstract::Adhesion of monocytes (U-937 cells) along a silicone tube deployed with circular ring stents was studied to test the hypothesis that by intentionally inducing swirling flow in an endovascular stent, the adhesion of monocytes would be suppressed. The results revealed that under a normal flow condition, the flow disturb...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-120622

    authors: Chen Z,Zhang X,Deng X

    更新日期:2012-01-01 00:00:00

  • The influence of temperature and length of time of storage of frog mucus samples.

    abstract::Frozen, stored mucus has been extensively used for transport studies but there is no clear evidence of the influence that the temperature and length of time of storage may have on the results. We stored frog mucus samples at -20 and -80 degrees C and analysed them on days zero, 2, 10, 30 and 90. At each temperature, a...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Gastaldi AC,Jardim JR,King M

    更新日期:2000-01-01 00:00:00

  • Filterability of sickle cells as a function of pO2: role of physico-chemical factors.

    abstract::A rigidity index (RI) related to red blood cell deformability was measured by using the hemorheometre. The RI for 13 patients homozygous for sickle cell disease was 109 +/- 44 at 37 degrees C and at atmospheric pO2. The filtration time curve as a function of pO2 is biphasic for sickle cell suspensions. The pO2 at whic...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1989-26409

    authors: Kraiem A,Craescu CT,Galacteros F,Martin-Caburi J,Domenget C,Garel MC,Beuzard Y

    更新日期:1989-01-01 00:00:00

  • Interactive role of tyrosine kinase, protein kinase C, and Rho/Rho kinase systems in the mechanotransduction of vascular smooth muscles.

    abstract::Blood vessels are always subjected to hemodynamic stresses including blood pressure and blood flow. The cerebral artery is particularly sensitive to hemodynamic stresses such as pressure and stretch, and shows contractions that are myogenic in nature; i.e., the mechanical response is generated by the vascular smooth m...

    journal_title:Biorheology

    pub_type: 杂志文章,评审

    doi:

    authors: Nakayama K,Obara K,Tanabe Y,Saito M,Ishikawa T,Nishizawa S

    更新日期:2003-01-01 00:00:00

  • Shear stress-induced binding of von Willebrand factor to platelets.

    abstract::Shear stress-induced platelet aggregation requires von Willebrand factor (vWF), platelet glycoprotein (GP) Ib, GPIIb-IIIa, Ca2+, and adenosine diphosphate (ADP). Recent reports using vWF labeled with either 125I or fluorescein isothiocyanate (FITC) have demonstrated that in shear-fields, vWF binds to both GPIb and GPI...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.1016/S0006-355X(97)00004-8

    authors: Konstantopoulos K,Chow TW,Turner NA,Hellums JD,Moake JL

    更新日期:1997-01-01 00:00:00