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Relative contribution of HIV-specific functional lymphocyte subsets restricted by protective and non-protective HLA alleles.

Abstract：

:Expression of major histocompatibility complex (MHC) class I alleles such as B*57 and B*27 are associated with slow HIV disease progression. HIV-specific immune responses in slow progressors (SP) are characterized by a poly-functional profile. We previously observed within infected subjects that HIV peptide-specific responses could differ from each other in their functional composition. We investigate here whether responses restricted by MHC class I alleles associated with slow disease progression have a more poly-functional profile than responses restricted by other alleles. We stimulated peripheral blood mononuclear cells (PBMCs) isolated from 36 chronically HIV-infected individuals with a panel of optimal peptides restricted by the HLA alleles expressed by each subject, and assessed the contribution of single IL-2-, single IFN-γ-, and IFN-γ/IL-2-secreting lymphocytes to the total response measured using a dual color ELISPOT assay. The contribution of functional subsets to responses restricted by HLA B*57/B*27 was similar in SP and progressors. For responses restricted by other MHC class I alleles, dual IFN-γ/IL-2-secreting lymphocytes contributed significantly more to the total response in SP than progressors. Within SP subjects, peptides restricted by both B*57/B*27 and other alleles stimulated responses with similar functional profiles. In progressors, peptides restricted by B*57/B*27 stimulated responses composed of a significantly greater proportion of IFN-γ/IL-2-secreting cells than peptides restricted by other alleles. Within progressors, the contribution of IFN-γ/IL-2-secreting lymphocytes was greater to epitopes restricted by protective HLA alleles compared with responses restricted by other alleles. HLA haplotypes influence the relative functional composition of HIV-specific responses.

journal_name

Viral Immunol

journal_title

Viral immunology

authors

Peretz Y,Marra O,Thomas R,Legault D,Côté P,Boulassel MR,Rouleau D,Routy JP,Sékaly RP,Tsoukas CM,Tremblay C,Bernard NF

doi

10.1089/vim.2010.0117

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

189-98

issue

eissn

0882-8245

issn

1557-8976

journal_volume

pub_type

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Relative contribution of HIV-specific functional lymphocyte subsets restricted by protective and non-protective HLA alleles.