Abstract:
:Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease with high morbidity and mortality. miR-182-5p is overexpressed in several fibrosis-related diseases but its effect in pulmonary fibrosis has not been reported yet. To investigate the function of miR-182-5p in pulmonary fibrosis, we established bleomycin (BLM)-induced fibrotic mice model and transforming growth factor-β1 (TGF-β1)-treated human embryonic lung fibroblasts model. In this study, miR-182-5p was highly expressed in pulmonary tissues of BLM-induced fibrotic mice. The content of hydroxyproline and TGF-β1 was decreased by downregulating the expression of miR-182-5p, indicating that fibrosis was alleviated in mice treated with Lentivirus-anti-miR-182-5p.Quantification of fibrosis-related proteins demonstrated that downregulation of miR-182-5p inhibited the expression of profibrotic proteins (fibronectin, α-smooth muscle actin, p-Smad2/p-Smad3) as well as enhanced the level of Smad7. In vitro assays validated that miR-182-5p was induced by TGF-β1 with the function of promoting fibrosis. In dual-luciferase reporter assay, Smad7 was demonstrated to be negatively regulated by miR-182-5p. Moreover, the effect of knocking down miR-182-5p on inhibiting fibrosis was achieved by upregulating the expression of Smad7. Therefore, miR-182-5p can be regarded as a biomarker of IPF and its inhibition may be a promising therapeutic approach in treating IPF.
journal_name
Hum Exp Toxicoljournal_title
Human & experimental toxicologyauthors
Chen Y,Zhang Q,Zhou Y,Yang Z,Tan Mdoi
10.1177/0960327119895549subject
Has Abstractpub_date
2020-05-01 00:00:00pages
683-695issue
5eissn
0960-3271issn
1477-0903journal_volume
39pub_type
杂志文章abstract::Chronic mercury exposure impairs vascular function, leading to the depression of endothelium-dependent vasodilatation. Loss of the nitric oxide (NO) pathway has been implicated, but little is known about effects on other endothelial mediators. This study investigated the mechanisms of endothelial dysfunction in rats s...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327110391389
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abstract:UNLABELLED:We present the first ever case of homicidal poisoning due to injection of methidathion, an organophosphate insecticide. CASE:A 4-month-old baby presented to the emergency department with chief complaints of unconsciousness and irregular respiration. A bitter odour and an injection site with a greenish blue ...
journal_title:Human & experimental toxicology
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journal_title:Human & experimental toxicology
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journal_title:Human & experimental toxicology
pub_type: 临床试验,杂志文章
doi:10.1177/096032719801700611
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journal_title:Human & experimental toxicology
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journal_title:Human & experimental toxicology
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doi:10.1177/0960327107083414
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journal_title:Human & experimental toxicology
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journal_title:Human & experimental toxicology
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journal_title:Human & experimental toxicology
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journal_title:Human & experimental toxicology
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pub_type: 临床试验,杂志文章
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journal_title:Human & experimental toxicology
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