Abstract:
:The presence of circulating permeability factors (cPFs) has been hypothesized to be associated with recurrence of focal segmental glomerulosclerosis (rFSGS) in renal allografts. The available methods to detect cPFs are complex, not easily repeatable and inappropriate to represent the anatomical characteristics of the three-layer glomerular filtration barrier (GFB). Here we describe a novel method which measures the permeability to bovine serum albumin (BSA) through a three-layer device (3LD). The 3 layers comprise: (1) conditionally immortalized human podocytes (HCiPodo), (2) collagen type IV coated porous membrane and (3) human glomerular endothelial cells (HCiGEnC). Using this method, we found that sera from all rFSGS patients increased albumin permeability, while sera from non recurrent (nrFSGS) and genetic (gFSGS) forms of FSGS did not. The mechanisms underlying the increase of albumin permeability are probably due to endothelial cell damage as an initial event, which was demonstrated by the decrease of Platelet endothelial cell adhesion molecule (PECAM-1 or CD31), while the podocytes' expressions of synaptopodin and podocin were normal. Furthermore, we also found that the plasmapheretic treatment (PPT) eliminated the effect of increasing BSA permeability in sera from rFSGS patients. These preliminary data suggest that our in vitro GFB model could not only be useful in predicting the recurrence of FSGS after renal transplantation (RTx), but also be a valuable in vitro model to study podocyte and endothelial cell biology.
journal_name
J Nephroljournal_title
Journal of nephrologyauthors
Li M,Alfieri CM,Morello W,Cellesi F,Armelloni S,Mattinzoli D,Montini G,Messa Pdoi
10.1007/s40620-019-00683-2subject
Has Abstractpub_date
2020-08-01 00:00:00pages
747-755issue
4eissn
1121-8428issn
1724-6059pii
10.1007/s40620-019-00683-2journal_volume
33pub_type
杂志文章abstract:BACKGROUND:Acute kidney injury (AKI) following acute ST elevation myocardial infarction (STEMI) is associated with adverse outcomes. The recently proposed KDIGO criteria suggested modifications to the consensus classification system for AKI, namely lowering the threshold of increase in absolute serum creatinine and ext...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.1007/s40620-017-0461-3
更新日期:2018-06-01 00:00:00
abstract::IgM secreting myelomas or lymphomas, including Waldenström macroglobulinemia, are associated with a varied spectrum of renal pathology, including intracapillary hyaline deposits, cryoglobulin, membranoproliferative glomerulonephritis, amyloid, monoclonal immunoglobulin deposition disease, cast nephropathy, and lymphom...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.1007/s40620-018-0544-9
更新日期:2018-12-01 00:00:00
abstract:BACKGROUND:Changes in podocyte phenotype and function are characteristic of proteinuric glomerular diseases. Integrin-linked kinase (ILK) functions as a common downstream effector in proteinuric diseases. In addition, ILK was shown to interact with the Wnt signaling pathway. Here, we investigated ILK expression as well...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.5301/jn.5000017
更新日期:2012-05-01 00:00:00
abstract::Preeclampsia (PE) is a protean syndrome causing a transitory kidney disease, characterised by hypertension and proteinuria, ultimately reversible after delivery. Its prevalence is variously estimated, from 3 to 5% to 10% if all the related disorders, including also pregnancy-induced hypertension (PIH) and HELLP syndro...
journal_title:Journal of nephrology
pub_type: 杂志文章,实务指引,评审
doi:10.1007/s40620-017-0390-1
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND:Since dialysis has brought long-term survival to uremic patients, we can now speculate on more subtle problems derived from imbalance or sub-optimal regulation of some elements such as trace metals. We focused on the rubidium (Rb) status in dialysis patients (HD), as concerns about its possible deficiency ha...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract:AIMS:The management of chronic kidney disease (CKD) complications is not always adequate in patients with a failed kidney transplant. We aimed to evaluate the frequency of CKD complications and assess whether they may lead to worse outcomes in this patient population. METHOD:We analyzed 49 kidney transplant recipients...
journal_title:Journal of nephrology
pub_type: 杂志文章,多中心研究
doi:10.1007/s40620-017-0449-z
更新日期:2018-02-01 00:00:00
abstract::The Liber medicine orinalibus (codex 69 Montecassino) of Hermogenes is the first known manuscript to have a Latin translation from the original Greek work of Magnus of Emesa (or Nisibis). The particular text here translated, from the so-called Commentatio, mentions direct transliteration of Greek concepts such as chym...
journal_title:Journal of nephrology
pub_type: 历史文章,杂志文章
doi:10.5301/JN.2011.6461
更新日期:2011-05-01 00:00:00
abstract:BACKGROUND:Proteinuria results from increased glomerular permeability and is associated with retraction and effacement of the highly specialized interdigitating podocyte foot processes. In podocytes (glomerular epithelial cells [GECs]), p130Cas localizes diffusely to the cytoplasm and connects focal adhesion proteins a...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.5301/jn.5000261
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVES:HERMEX is a population-based study which tries to evaluate the relative weight of cardiovascular risk factors in inhabitants of Extremadura, Spain. This report presents the data about chronic kidney disease (CKD) in a Spanish population sample. METHODS:For an observational cross-sectional population-based s...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.5301/jn.5000221
更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:Recently, some studies have emphasized the role of plasma 25-(OH)vitamin D (25OHD) levels in mineral metabolism dysregulation in chronic kidney diseases (CKDs). However, to date little attention has been paid to 25OHD metabolism abnormalities after renal transplantation (Tx). This cross-sectional study aimed...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::This review summarizes the plausible mechanisms of carcinogenesis, critically analyzes the literature on cancer risk and discusses issues of cancer screening in chronic dialysis patients. Despite conflicting results among various studies, there is sufficient evidence to conclude that there is a heightened incidence of...
journal_title:Journal of nephrology
pub_type: 杂志文章,评审
doi:
更新日期:2008-03-01 00:00:00
abstract:BACKGROUND:The relationship between malnutrition in hemodialysis (HD) patients with a chronic inflammatory condition and the expression levels of leukocyte integrins and their adhesiveness to fibronectin was investigated. METHODS:Subjective global assessment, albumin and body mass index were used as nutritional marker...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2010-03-01 00:00:00
abstract::Jaundice and pancreatitis are serious complications in post renal transplantations; hepatotropic viruses and drugs are commonly implicated. We describe ascaris infestation causing pancreatitis and obstructive jaundice in a renal transplant patient. ...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND:Accumulating experimental and clinical evidence reveals beneficial effects of n-3 polyunsaturated fatty acids (PUFAs) in kidney disease by modulating inflammation and fibrosis mechanisms that lead to renal failure. METHODS:EPA, DHA (n-3 PUFAs) and AA (n-6 PUFA) effects, compared to those of AngII, on renal ...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.5301/jn.5000193
更新日期:2013-07-01 00:00:00
abstract::Von Hippel-Lindau (VHL) disease is a rare autosomal dominant disorder characterized by multifocal and bilateral renal cell carcinoma and cysts, retinal angiomas, hemangioblastoma of the central nervous system, pheochromocytoma, epididymis cystoadenoma, pancreatic cysts and/or islet cell tumors. However, phenotypic man...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND:The effect of interleukin-11 (IL-11) on transforming growth factor-ß (TGF-ß) is controversial and has not been examined in renal diseases. In this study, we (i) characterised the up-regulation of TGF-ß1, phospho-p38 MAPK (p-p38 MAPK) and extracellular matrix during pathogenesis of glomerulonephritis and (ii)...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.5301/jn.2010.5094
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:The most frequent cause of death in hemodialysis (HD) patients is cardiovascular disease (CVD), and chronic inflammation has been identified as an epidemiologically important risk factor for CVD. Elevated levels of minor acute phase reactants, such as ceruloplasmin (Cp) and transferrin, have been related to ...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
abstract::Renal fibrogenesis can be induced by several injury mechanisms in different renal diseases, but ultimately produces identical fibrotic changes in the kidney. Recently, a number of agents that can inhibit extracellular matrix (ECM) accumulation have been studied, suggesting a therapeutic utility in the treatment of fib...
journal_title:Journal of nephrology
pub_type: 杂志文章,评审
doi:
更新日期:2004-07-01 00:00:00
abstract:BACKGROUND:Different mitogens are involved in the pathogenesis of kidney damage after subtotal nephrectomy. One of them, TGF-beta, controls mesangial cell proliferation and interstitial fibrosclerosis. The transduction of the TGF-beta signal is controlled by intracellular signalling molecules such as Ras G monomeric pr...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::Recent molecular cloning experiments have identified the existence of four NBC isoforms (NBC1, 2, 3 and 4) and two NBC-related proteins AE4 and NCBE (Anion Exchanger 4 and Sodium-dependent Chloride-Bicarbonate Exchanger). Except for AE4 which does not transport Na, all are presumed to mediate the cotransport of Na+ an...
journal_title:Journal of nephrology
pub_type: 杂志文章,评审
doi:
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:Because urinary low molecular weight protein (LMWP) measurement shows changes in renal integrity at an early stage, beta2-microglobulin (B2m), retinol-binding protein (RBP) and alpha1-microglobulin (A1m) were evaluated in 24-hour urine collection of 65 patients with pure monoclonal light chain (MLC) proteinu...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2007-11-01 00:00:00
abstract::Epicardial adipose tissue (EAT) is localized between the myocardial surface and visceral layer of the pericardium. It is a metabolically active organ that secretes several cytokines which modulate cardiovascular morphology and function. EAT may interact locally with coronary arteries through paracrine secretion mechan...
journal_title:Journal of nephrology
pub_type: 杂志文章,评审
doi:10.1007/s40620-018-0491-5
更新日期:2018-12-01 00:00:00
abstract::The pathogenesis of calcium-oxalate (CaOx) renal stones is still debated and a number of issues needs to be clarified. In particular, it is difficult to combine the intraluminal physical-chemical imbalance and fixed particle theory with the evidence that CaOx stones actually form and grow on Randall's plaque in the re...
journal_title:Journal of nephrology
pub_type: 杂志文章,评审
doi:
更新日期:2004-11-01 00:00:00
abstract::Icodextrin, a glucose polymer, is widely used as an alternative to glucose as the osmotic agent in peritoneal dialysis (PD). We describe a case of a continuous ambulatory peritoneal dialysis patient who developed severe cutaneous hypersensitivity after initiation of icodextrin PD solution. Erythematous skin lesions gr...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2006-09-01 00:00:00
abstract::The kidney does not usually present specific lesions in cystic fibrosis (CF), although in recent years renal involvement has been reported, particularly amyloidosis and immune complex glomerulonephritis. IgA nephropathy is rare. We report four cases of IgA nephropathy out of five renal biopsies performed in the last t...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND AND AIMS:Reduction of intestinal load of phosphorus is important for the prevention and treatment of chronic kidney disease (CKD)-mineral and bone disorder (MBD). However, this strategy is limited by patients' poor adherence to dietary prescription and by the existence of hidden sources of phosphorus. In add...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.1007/s40620-016-0306-5
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:Gut-derived uremic toxins have been associated with adverse outcomes in chronic kidney disease (CKD). Alterations in bowel habits, including constipation, seem to play an additional role in uremic toxicity. The aim of this study is to investigate the association of bowel habits with gut-derived uremic toxins...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.1007/s40620-020-00819-9
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVE:To investigate the pregnancy outcomes for patients with stage 3-4 chronic kidney disease (CKD) and the effects of pregnancy on kidney function. METHODS:Clinical data of pregnant women with CKD in the Peking University First Hospital between January 1st 2005 and October 1st 2016 were retrospectively analysed....
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.1007/s40620-018-0509-z
更新日期:2018-12-01 00:00:00
abstract:BACKGROUND:The G-1082A polymorphism of the interleukin-10 (IL-10) gene has been associated with modified gene expression and the progression of primary IgA nephropathy (IgAN). In the present study, we evaluated its influence on recurrent IgAN after renal transplantation. METHODS:We studied 103 patients who suffered fr...
journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2008-11-01 00:00:00
abstract::Survival analysis is the statistical method for studying the time between entry to a study and a subsequent event. Survival is often the most important outcome in both observational and intervention studies. The analysis of survival, however, is not simple because of a number of factors. The time between entry to a st...
journal_title:Journal of nephrology
pub_type: 杂志文章,评审
doi:
更新日期:2004-03-01 00:00:00