Abstract:
BACKGROUND:Uromodulin (Tamm-Horsfall protein) is the most abundant urinary protein in healthy individuals. Despite 60 years of research, its physiological role remains rather elusive. Familial juvenile hyperuricemic nephropathy and medullary cystic kidney disease Type 2 are autosomal dominant tubulointerstitial nephropathies characterized by gouty arthritis and progressive renal insufficiency, caused by uromodulin (UMOD) mutations. The aim of this study was to compare the cellular effects of mutant and wild-type UMOD. METHODS:Wild-type UMOD cDNA was cloned from human kidney cDNA into pcDNA3 expression vector. A mutant UMOD construct, containing the previously reported mutation, V273, was created by in vitro mutagenesis. Transient and stable transfection studies were performed in human embryonic kidney cells and mouse distal convoluted tubular cells, respectively. Expression was evaluated by reverse transcription polymerase chain reaction (RT-PCR), western blot and immunofluorescence. Oligosaccharide cleavage by glycosidases was performed to characterize different forms of UMOD. Nuclear translocation of P65 and degradation of IκBα and IRAK1 in response to interleukin (IL)-1β were used to evaluate the effects of wild-type and mutant UMOD on the IL-1R-NFκB pathway. RESULTS:The mutant protein was shown to be retained in the endoplasmic reticulum and was not excreted to the cell medium, as opposed to the wild-type protein. NFκB activation in cells expressing mutant UMOD was similar to that of untransfected cells. In contrast, cells over-expressing wild-type UMOD showed markedly reduced NFκB activation. CONCLUSION:Our findings suggest that UMOD may have a physiologic function related to its inhibitory effect on the NFκB pathway.
journal_name
J Nephroljournal_title
Journal of nephrologyauthors
Dinour D,Ganon L,Nomy LI,Ron R,Holtzman EJdoi
10.1007/s40620-014-0079-7subject
Has Abstractpub_date
2014-06-01 00:00:00pages
257-64issue
3eissn
1121-8428issn
1724-6059journal_volume
27pub_type
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pub_type: 杂志文章,meta分析
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pub_type: 临床试验,杂志文章
doi:
更新日期:2007-07-01 00:00:00
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更新日期:2004-09-01 00:00:00
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更新日期:2009-11-01 00:00:00
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doi:10.5301/jn.5000025
更新日期:2011-11-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2016-08-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:2008-05-01 00:00:00
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更新日期:2010-09-01 00:00:00
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更新日期:2008-01-01 00:00:00
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