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Proteomic analyses of the SMYD family interactomes identify HSP90 as a novel target for SMYD2.

Abstract：

:The SMYD (SET and MYND domain) family of lysine methyltransferases (KMTs) plays pivotal roles in various cellular processes, including gene expression regulation and DNA damage response. Initially identified as genuine histone methyltransferases, specific members of this family have recently been shown to methylate non-histone proteins such as p53, VEGFR, and the retinoblastoma tumor suppressor (pRb). To gain further functional insights into this family of KMTs, we generated the protein interaction network for three different human SMYD proteins (SMYD2, SMYD3, and SMYD5). Characterization of each SMYD protein network revealed that they associate with both shared and unique sets of proteins. Among those, we found that HSP90 and several of its co-chaperones interact specifically with the tetratrico peptide repeat (TPR)-containing SMYD2 and SMYD3. Moreover, using proteomic and biochemical techniques, we provide evidence that SMYD2 methylates K209 and K615 on HSP90 nucleotide-binding and dimerization domains, respectively. In addition, we found that each methylation site displays unique reactivity in regard to the presence of HSP90 co-chaperones, pH, and demethylation by the lysine amine oxidase LSD1, suggesting that alternative mechanisms control HSP90 methylation by SMYD2. Altogether, this study highlights the ability of SMYD proteins to form unique protein complexes that may underlie their various biological functions and the SMYD2-mediated methylation of the key molecular chaperone HSP90.

journal_name

J Mol Cell Biol

journal_title

Journal of molecular cell biology

authors

Abu-Farha M,Lanouette S,Elisma F,Tremblay V,Butson J,Figeys D,Couture JF

doi

10.1093/jmcb/mjr025

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

301-8

issue

5

eissn

1674-2788

issn

1759-4685

pii

mjr025

journal_volume

3

pub_type

杂志文章

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