Microcircuits of excitatory and inhibitory neurons in layer 2/3 of mouse barrel cortex.

Abstract:

:Synaptic interactions between nearby excitatory and inhibitory neurons in the neocortex are thought to play fundamental roles in sensory processing. Here, we have combined optogenetic stimulation, whole cell recordings, and computational modeling to define key functional microcircuits within layer 2/3 of mouse primary somatosensory barrel cortex. In vitro optogenetic stimulation of excitatory layer 2/3 neurons expressing channelrhodopsin-2 evoked a rapid sequence of excitation followed by inhibition. Fast-spiking (FS) GABAergic neurons received large-amplitude, fast-rising depolarizing postsynaptic potentials, often driving action potentials. In contrast, the same optogenetic stimulus evoked small-amplitude, subthreshold postsynaptic potentials in excitatory and non-fast-spiking (NFS) GABAergic neurons. To understand the synaptic mechanisms underlying this network activity, we investigated unitary synaptic connectivity through multiple simultaneous whole cell recordings. FS GABAergic neurons received unitary excitatory postsynaptic potentials with higher probability, larger amplitudes, and faster kinetics compared with NFS GABAergic neurons and other excitatory neurons. Both FS and NFS GABAergic neurons evoked robust inhibition on postsynaptic layer 2/3 neurons. A simple computational model based on the experimentally determined electrophysiological properties of the different classes of layer 2/3 neurons and their unitary synaptic connectivity accounted for key aspects of the network activity evoked by optogenetic stimulation, including the strong recruitment of FS GABAergic neurons acting to suppress firing of excitatory neurons. We conclude that FS GABAergic neurons play an important role in neocortical microcircuit function through their strong local synaptic connectivity, which might contribute to driving sparse coding in excitatory layer 2/3 neurons of mouse barrel cortex in vivo.

journal_name

J Neurophysiol

authors

Avermann M,Tomm C,Mateo C,Gerstner W,Petersen CC

doi

10.1152/jn.00917.2011

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

3116-34

issue

11

eissn

0022-3077

issn

1522-1598

pii

jn.00917.2011

journal_volume

107

pub_type

杂志文章
  • EMG characteristics of the external anal sphincter guarding reflex and effects of a unilateral ventral root avulsion injury in rhesus macaques ( Macaca mulatta).

    abstract::The external anal sphincter (EAS) is important for the maintenance of bowel continence and may be compromised by a variety of neuropathic conditions. However, large animal models for the study of EAS functions have been sparse. The EAS guarding reflex was examined by electromyography (EMG) in neurologically intact rhe...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00435.2017

    authors: Chang HH,Lee U,Vu T,Pikov V,Nieto JH,Christe KL,Havton LA

    更新日期:2018-12-01 00:00:00

  • Cerebellar contributions to self-motion perception: evidence from patients with congenital cerebellar agenesis.

    abstract::The cerebellum was historically considered a brain region dedicated to motor control, but it has become clear that it also contributes to sensory processing, particularly when sensory discrimination is required. Prior work, for example, has demonstrated a cerebellar contribution to sensory discrimination in the visual...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00763.2015

    authors: Dahlem K,Valko Y,Schmahmann JD,Lewis RF

    更新日期:2016-05-01 00:00:00

  • Recovery of contralesional saccade choice and reaction time deficits after a unilateral endothelin-1-induced lesion in the macaque caudal prefrontal cortex.

    abstract::The caudal primate prefrontal cortex (PFC) is involved in target selection and visually guided saccades through both covert attention and overt orienting eye movements. Unilateral damage to the caudal PFC often leads to decreased awareness of a contralesional target alone, referred to as "neglect," or when it is prese...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00078.2019

    authors: Adam R,Johnston K,Everling S

    更新日期:2019-08-01 00:00:00

  • Plateau pattern of afferent discharge rate from frog muscle spindles.

    abstract::1. A characteristic plateau pattern was observed in the rate of afferent discharges during ramp-and-hold stretch of spindles isolated from semitendinosus muscles of frogs. The plateau pattern was more frequent in summer frogs (84%) than winter frogs (11%). 2. The plateau pattern consisted of a discharge rate around 12...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1993.70.1.275

    authors: Sokabe M,Nunogaki K,Naruse K,Soga H,Fujitsuka N,Yoshimura A,Ito F

    更新日期:1993-07-01 00:00:00

  • Neurons in the human hippocampus and amygdala respond to both low- and high-level image properties.

    abstract::A large number of studies have demonstrated that structures within the medial temporal lobe, such as the hippocampus, are intimately involved in declarative memory for objects and people. Although these items are abstractions of the visual scene, specific visual details can change the speed and accuracy of their recal...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00977.2010

    authors: Steinmetz PN,Cabrales E,Wilson MS,Baker CP,Thorp CK,Smith KA,Treiman DM

    更新日期:2011-06-01 00:00:00

  • Odor specificity of habituation in the rat anterior piriform cortex.

    abstract::Exposure to odorants results in a rapid (<10 s) reduction in odor-evoked activity in the rat piriform cortex despite relatively maintained afferent input from olfactory bulb mitral cells. To further understand this form of cortical plasticity, a detailed analysis of its odor specificity was performed. Habituation of o...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2000.83.1.139

    authors: Wilson DA

    更新日期:2000-01-01 00:00:00

  • Facilitation at the lobster neuromuscular junction: a stimulus-dependent mobilization model.

    abstract::Frequency facilitation is a process whereby neurosecretion increases as a function of stimulation frequency during repetitive synaptic activity. To examine the physiological basis underlying facilitation, we have estimated the frequency dependence of the synaptic parameters n (number of units capable of responding to ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1997.78.1.417

    authors: Worden MK,Bykhovskaia M,Hackett JT

    更新日期:1997-07-01 00:00:00

  • Joint and separate effects of estrogen and progesterone on responses of midbrain neurons to lordosis-controlling somatic stimuli in the female golden Syrian hamster.

    abstract::Single midbrain neurons were examined for effects of ovarian hormone administration on responsiveness to an array of lordosis-controlling types of somatosensory stimuli in anesthetized, ovariectomized Syrian hamsters. Neuronal responses were recorded under four different hormonal treatment conditions: 1) estradiol ben...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1984.51.5.1040

    authors: Rose JD,Bieber SL

    更新日期:1984-05-01 00:00:00

  • Encoding of compressive stress during indentation by slowly adapting type I mechanoreceptors in rat hairy skin.

    abstract::The mechanical state encoded by slowly adapting type 1 mechanoreceptors (SAI) during indentation was examined using an isolated preparation in a rat model. Skin and its intact innervation were harvested from the medial thigh of the rat hindlimb and placed in a dish, with the corium side down, containing synthetic inte...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00414.2001

    authors: Ge W,Khalsa PS

    更新日期:2002-04-01 00:00:00

  • Sensory processing in a thermal afferent pathway.

    abstract::Extracellular recordings were made from cold-receptive afferent fibers in the trigeminal ganglion of rats anesthetized with halothane. By applying a standardized series of steady or changing temperatures to the receptive fields, we recorded the static and dynamic responses of the afferents. Comparable recordings were ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1985.53.2.429

    authors: Davies SN,Goldsmith GE,Hellon RF,Mitchell D

    更新日期:1985-02-01 00:00:00

  • Mapping by laser photostimulation of connections between the thalamic reticular and ventral posterior lateral nuclei in the rat.

    abstract::We used laser scanning photostimulation through a focused UV laser of caged glutamate in an in vitro slice preparation through the rat's somatosensory thalamus to study topography and connectivity between the thalamic reticular nucleus and ventral posterior lateral nucleus. This enabled us to focally stimulate the som...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00206.2005

    authors: Lam YW,Sherman SM

    更新日期:2005-10-01 00:00:00

  • Event-related potentials elicited by errors during the stop-signal task. II: human effector-specific error responses.

    abstract::Although previous research with human and nonhuman primates has examined the neural correlates of performance monitoring, discrepancies in methodology have limited our ability to make cross-species generalizations. One major obstacle arises from the use of different behavioral responses and tasks across different prim...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00803.2011

    authors: Reinhart RM,Carlisle NB,Kang MS,Woodman GF

    更新日期:2012-05-01 00:00:00

  • Sound-level-dependent representation of frequency modulations in human auditory cortex: a low-noise fMRI study.

    abstract::Recognition of sound patterns must be largely independent of level and of masking or jamming background sounds. Auditory patterns of relevance in numerous environmental sounds, species-specific vocalizations and speech are frequency modulations (FM). Level-dependent activation of the human auditory cortex (AC) in resp...

    journal_title:Journal of neurophysiology

    pub_type: 临床试验,杂志文章

    doi:10.1152/jn.00187.2001

    authors: Brechmann A,Baumgart F,Scheich H

    更新日期:2002-01-01 00:00:00

  • Premotor cortex of monkeys: set- and movement-related activity reflecting amplitude and direction of wrist movements.

    abstract::1. Neuronal activity was recorded from the premotor cortex (PM) of Japanese monkeys while they performed hand movements with different amplitudes and directions. On each behavioral trial, two instructions were given sequentially: 1) an amplitude instruction (large or small) and 2) a direction instruction (flexion or e...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1993.69.1.187

    authors: Kurata K

    更新日期:1993-01-01 00:00:00

  • Spatiotemporally graded NMDA spike/plateau potentials in basal dendrites of neocortical pyramidal neurons.

    abstract::Glutamatergic inputs clustered over approximately 20-40 microm can elicit local N-methyl-D-aspartate (NMDA) spike/plateau potentials in terminal dendrites of cortical pyramidal neurons, inspiring the notion that a single terminal dendrite can function as a decision-making computational subunit. A typical terminal basa...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00011.2008

    authors: Major G,Polsky A,Denk W,Schiller J,Tank DW

    更新日期:2008-05-01 00:00:00

  • Descending projections from the nucleus accumbens shell excite activity of taste-responsive neurons in the nucleus of the solitary tract in the hamster.

    abstract::The nucleus of the solitary tract (NST) and the parabrachial nuclei (PbN) are the first and second relays in the rodent central taste pathway. A series of electrophysiological experiments revealed that spontaneous and taste-evoked activities of brain stem gustatory neurons are altered by descending input from multiple...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00362.2014

    authors: Li CS,Lu DP,Cho YK

    更新日期:2015-06-01 00:00:00

  • Quantitative characterization of ceruleospinal inhibition of nociceptive transmission in the rat.

    abstract::A total of 51 dorsal horn units responsive to heat were isolated and their receptive fields characterized (i.e., response properties and adequate stimuli determined) in pentobarbital-anesthetized, paralyzed rats. In 39 of the 51 units, the descending inhibition of heat-evoked activity produced by focal electrical stim...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1986.56.5.1397

    authors: Jones SL,Gebhart GF

    更新日期:1986-11-01 00:00:00

  • Effects of caudate microstimulation on spontaneous and purposive saccades.

    abstract::Electrical stimulation has been delivered to the basal ganglia (BG) to treat intractable symptoms of a variety of clinical disorders. However, it is still unknown how such treatments improve behavioral symptoms. A difficulty of this problem is that artificial signals created by electrical stimulation interact with int...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00046.2013

    authors: Watanabe M,Munoz DP

    更新日期:2013-07-01 00:00:00

  • A biocompatible titanium headpost for stabilizing behaving monkeys.

    abstract::Many neurophysiological experiments involving monkeys require that the head be stabilized while the animal performs a task. Often a post is attached to the skull to accomplish this goal, using a headcap formed from dental acrylic. We describe a new headpost, developed by refinement of several prototypes, and supply an...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00102.2007

    authors: Adams DL,Economides JR,Jocson CM,Horton JC

    更新日期:2007-08-01 00:00:00

  • Contextual effects of noise on vocalization encoding in primary auditory cortex.

    abstract::Robust auditory perception plays a pivotal function for processing behaviorally relevant sounds, particularly with distractions from the environment. The neuronal coding enabling this ability, however, is still not well understood. In this study, we recorded single-unit activity from the primary auditory cortex (A1) o...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00476.2016

    authors: Ni R,Bender DA,Shanechi AM,Gamble JR,Barbour DL

    更新日期:2017-02-01 00:00:00

  • Activation kinetics of the delayed rectifier potassium current of bullfrog sympathetic neurons.

    abstract::We examined the activation kinetics of the delayed rectifier K+ current of bullfrog sympathetic neurons, primarily using whole cell recording. On depolarization, currents activated with a sigmoid delay but did not show a Cole-Moore shift. The time course of activation differed systematically from an exponential raised...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1998.79.5.2345

    authors: Klemic KG,Durand DM,Jones SW

    更新日期:1998-05-01 00:00:00

  • Properties of the GABAA receptor of rat posterior pituitary nerve terminals.

    abstract::1. We investigated gamma-aminobutyric acid (GABA) receptors using thin slice patch-clamp techniques in the swellings along axons of posterior pituitary nerve terminals. 2. Activation of the nerve terminal GABAA receptor induced a mean conductance change of 1.5 nS. Normalizing to area gave a mean conductance density of...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1995.73.3.1135

    authors: Zhang SJ,Jackson MB

    更新日期:1995-03-01 00:00:00

  • Augmented supraorbital skin sympathetic nerve activity responses to symptom trigger events in rosacea patients.

    abstract::Facial flushing in rosacea is often induced by trigger events. However, trigger causation mechanisms are currently unclear. This study tested the central hypothesis that rosacea causes sympathetic and axon reflex-mediated alterations resulting in trigger-induced symptomatology. Twenty rosacea patients and age/sex-matc...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00458.2015

    authors: Metzler-Wilson K,Toma K,Sammons DL,Mann S,Jurovcik AJ,Demidova O,Wilson TE

    更新日期:2015-09-01 00:00:00

  • Optogenetic fMRI sheds light on the neural basis of the BOLD signal.

    abstract::Blood oxygenation level dependent (BOLD) functional magnetic resonance imaging (fMRI) is widely used as a measure of neuronal activity, despite an incomplete understanding of the hemodynamic and neural bases for BOLD signals. Recent work by Lee and colleagues investigated whether activating genetically specified neuro...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章,评审

    doi:10.1152/jn.00535.2010

    authors: Palmer HS

    更新日期:2010-10-01 00:00:00

  • Frequency-dependent regulation of afferent transmission in the feeding circuitry of Aplysia.

    abstract::During rhythmic behaviors, sensori-motor transmission is often regulated so that there are phasic changes in afferent input to follower neurons. We study this type of regulation in the feeding circuit of Aplysia. We characterize effects of the B4/5 interneurons on transmission from the mechanoafferent B21 to the radul...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00786.2003

    authors: Evans CG,Jing J,Proekt A,Rosen SC,Cropper EC

    更新日期:2003-12-01 00:00:00

  • Respiratory-related neural assemblies in the brain stem midline.

    abstract::1. The initial objective of this study was to determine whether respiratory-related neural assemblies exist in the brain stem midline. A second goal was to seek evidence for concurrent relationships among the neurons that could generate the detected synchrony. 2. Experiments were conducted on anesthetized, paralyzed, ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1992.67.4.905

    authors: Lindsey BG,Hernandez YM,Morris KF,Shannon R,Gerstein GL

    更新日期:1992-04-01 00:00:00

  • Reward skewness coding in the insula independent of probability and loss.

    abstract::Rewards in the natural environment are rarely predicted with complete certainty. Uncertainty relating to future rewards has typically been defined as the variance of the potential outcomes. However, the asymmetry of predicted reward distributions, known as skewness, constitutes a distinct but neuroscientifically under...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00471.2011

    authors: Burke CJ,Tobler PN

    更新日期:2011-11-01 00:00:00

  • The parabrachial area: electrophysiological evidence for an involvement in visceral nociceptive processes.

    abstract::1. Neurons (n = 142) were recorded with extracellular micropipettes in the parabrachial (PB) area, in the anesthetized rat, some of them being antidromically driven from the centralis nucleus of the amygdala (Ce). The spontaneous activity of these neurons was low, (10th percentile < median frequency < 90th percentile;...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1994.71.5.1646

    authors: Bernard JF,Huang GF,Besson JM

    更新日期:1994-05-01 00:00:00

  • Ca2+-induced uncoupling of Aplysia bag cell neurons.

    abstract::Electrical transmission is a dynamically regulated form of communication and key to synchronizing neuronal activity. The bag cell neurons of Aplysia are a group of electrically coupled neuroendocrine cells that initiate ovulation by secreting egg-laying hormone during a prolonged period of synchronous firing called th...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00603.2014

    authors: Dargaei Z,Standage D,Groten CJ,Blohm G,Magoski NS

    更新日期:2015-02-01 00:00:00

  • A motor area rostral to the supplementary motor area (presupplementary motor area) in the monkey: neuronal activity during a learned motor task.

    abstract::1. The rostromesial agranular frontal cortex of macaque monkey (Macaca fuscata), traditionally defined as the supplementary motor area (SMA), was studied using various physiological techniques to delineate two different areas rostrocaudally. 2. Field and unitary responses to electrical stimulation of the primary motor...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1992.68.3.653

    authors: Matsuzaka Y,Aizawa H,Tanji J

    更新日期:1992-09-01 00:00:00