The therapeutic effect of verapamil in lipopolysaccharide-induced acute lung injury.

abstract：:Hsp16.3, a small heat shock protein from Mycobacterium tuberculosis (MTB), was originally identified as an immuno-dominant antigen and later found to be a major membrane protein. In vitro studies show that Hsp16.3 exists as nonamers and undergoes dynamic dissociation/re-association equilibrium in solutions. Neverthele...

journal_title：Biochemical and biophysical research communications

authors： Zhang H,Fu X,Jiao W,Zhang X,Liu C,Chang Z

更新日期：2005-05-20 00:00:00

abstract：:We investigated the presence of the carbohydrate ligand for E-selectin on the cell surface of rat polymorphonuclear leukocytes (PMN). Rat PMN, isolated from peripheral blood, adhered to recombinant rat E-selectin-coated microplates. The adhesion was inhibited either by an anti-rat E-selectin monoclonal antibody (MAb),...

journal_title：Biochemical and biophysical research communications

authors： Misugi E,Kawamura N,Imanishi N,Tojo SJ,Morooka S

更新日期：1995-10-13 00:00:00

abstract：:We developed a typing assay for HIV-1 using subtype specific peptides corresponding to the five major subtypes of HIV-1 (A to E). In eight patients serologically subtyped as A (n = 1), B (n = 3), C (n = 3) and E (n = 1) phyllogenetic analysis of sequenced V3 domain DNA completely correlated to the peptide serotyping. ...

journal_title：Biochemical and biophysical research communications

authors： Sherefa K,Sönnerborg A,Steinbergs J,Sällberg M

更新日期：1994-12-30 00:00:00

abstract：:The left-handed Z-DNA form of the short unmodified alternating guanine-cytosine oligonucleotides, 5'-(dGdC)(24) and 5'-(dGdC)(18), was selectively detected under physiological ionic strength and pH conditions using the anionic nickel(II) porphyrin, NiTPPS. No spectroscopic signal was observed for NiTPPS with any right...

journal_title：Biochemical and biophysical research communications

authors： D'Urso A,Choi JK,Shabbir-Hussain M,Ngwa FN,Lambousis MI,Purrello R,Balaz M

更新日期：2010-06-25 00:00:00

abstract：:Myofibrillogenesis regulator-1(MR-1) can aggravate cardiac hypertrophy induced by angiotensin(Ang) II in mice through activation of NF-kappaB signaling pathway, and nuclear transcription factor (NF)-kappaB and activator protein-1(AP-1) regulate inflammatory and immune responses by increasing the expression of specific...

journal_title：Biochemical and biophysical research communications

authors： Dai W,Chen H,Jiang J,Kong W,Wang Y

更新日期：2010-01-15 00:00:00

abstract：:Based on pseudo amino acid (PseAA) composition and a novel hybrid feature selection frame, this paper presents a computational system to predict the PPIs (protein-protein interactions) using 8796 protein pairs. These pairs are coded by PseAA composition, resulting in 114 features. A hybrid feature selection system, mR...

journal_title：Biochemical and biophysical research communications

authors： Liu L,Cai Y,Lu W,Feng K,Peng C,Niu B

更新日期：2009-03-06 00:00:00

abstract：:We quantified the damage yields produced in plasmid DNA by γ-irradiation in the presence of low concentrations (10-100 μM) of ascorbic acid, which is a major antioxidant in living systems, to clarify whether it chemically repairs radiation damage in DNA. The yield of DNA single strand breaks induced by irradiation was...

journal_title：Biochemical and biophysical research communications

authors： Hata K,Urushibara A,Yamashita S,Shikazono N,Yokoya A,Katsumura Y

更新日期：2013-05-03 00:00:00

abstract：:Ascorbate peroxidase (APX) is a hydrogen peroxide-scavenging peroxidase which uses ascorbate (AsA) as the specific electron donor. APX has not been isolated in mammals. Ocular tissue contains AsA at high concentrations, and we detected APX activity in bovine retinal pigment epithelium (RPE) and choroid. We purified AP...

journal_title：Biochemical and biophysical research communications

authors： Wada N,Kinoshita S,Matsuo M,Amako K,Miyake C,Asada K

更新日期：1998-01-14 00:00:00

abstract：:The CitA citrate transporter in Salmonella typhimurium is encoded by the citA gene and consists of 434 amino acid residues that probably include 12 membrane-spanning segments [Shimamoto. T., et al. (1991) J. Biochem. 110, 22-28]. CitA mutants with altered substrate specificities were isolated by in vitro mutagenesis u...

journal_title：Biochemical and biophysical research communications

authors： Shimamoto T,Negishi K,Tsuda M,Tsuchiya T

更新日期：1996-09-13 00:00:00

abstract：:Retinoic acid (RA) is described as an inhibitor of prostate cancer cell growth. We utilized reverse transcription-polymerase chain reaction (RT-PCR) to analyze expression of different isoforms of fibroblast growth factor 8 (FGF8) in response to RA. Results in the prostate cancer cell line LNCaP show that whereas overa...

journal_title：Biochemical and biophysical research communications

authors： Brondani V,Hamy F

更新日期：2000-05-27 00:00:00

abstract：:Effect of epsilon subunit on the nucleotide binding to the catalytic sites of F(1)-ATPase from the thermophilic Bacillus PS3 (TF(1)) has been tested by using alpha(3)beta(3)gamma and alpha(3)beta(3)gammaepsilon complexes of TF(1) containing betaTyr341 to Trp substitution. The nucleotide binding was assessed with fluor...

journal_title：Biochemical and biophysical research communications

authors： Yasuno T,Muneyuki E,Yoshida M,Kato-Yamada Y

更新日期：2009-12-11 00:00:00

abstract：:Calcium regulates diverse cellular activities in plants through the action of calmodulin (CaM). By using (35)S-labeled CaM to screen an Arabidopsis seedling cDNA expression library, a cDNA designated as AtCh-CPN10 (Arabidopsis thaliana chloroplast chaperonin 10) was cloned. Chloroplast CPN10, a nuclear-encoded protein...

journal_title：Biochemical and biophysical research communications

authors： Yang T,Poovaiah BW

更新日期：2000-08-28 00:00:00

abstract：:We have identified by protein sequencing the precursor of beta protein in newborn rat brain. A rabbit antibody was raised against a synthetic peptide corresponding to the carboxyl-terminal 30 residues (666-695) of the putative beta-amyloid protein precursor (Kang et al. 1987). The antiserum recognized multiple bands a...

journal_title：Biochemical and biophysical research communications

authors： Takio K,Hasegawa M,Titani K,Ihara Y

更新日期：1989-05-15 00:00:00

abstract：:We have recorded multi-frequency EPR spectra of 63Cu- and 65Cu-labeled, water-soluble CuA-protein from the cytochrome ba3 of T. thermophilus. The spectrum taken at the highest frequency (34.03 GHz) shows no hyperfine structure and is nominally axial with apparent gz approximately 2.18 and gxy approximately 2.00. The s...

journal_title：Biochemical and biophysical research communications

authors： Fee JA,Sanders D,Slutter CE,Doan PE,Aasa R,Karpefors M,Vänngård T

更新日期：1995-07-06 00:00:00

abstract：:Using isolated liver mitochondria we show that low concentrations of TBT (0.5 microM) cause the release of mitochondrial cytochrome c, in the presence of Ca(2+). This is reflected in a rapid loss of membrane potential (DeltaPsi(m)), and a large-amplitude swelling characteristic of mitochondrial permeability transition...

journal_title：Biochemical and biophysical research communications

authors： Gogvadze V,Stridh H,Orrenius S,Cotgreave I

更新日期：2002-04-12 00:00:00

abstract：:Aqueous extracts of cigarette tar produce hydroxyl radicals that are spin trapped by 5,5-dimethyl-1-pyrroline-N-oxide. The addition of catalase almost completely inhibits and superoxide dismutase partially inhibits spin adduct formation. The addition of ethylenediamine tetraacetic acid greatly increases the amount of ...

journal_title：Biochemical and biophysical research communications

authors： Cosgrove JP,Borish ET,Church DF,Pryor WA

更新日期：1985-10-15 00:00:00

abstract：:Two genes expressing 70 kDa heat shock proteins were identified in Cyprinus carpio. The sequence similarities and the intron-interrupted structure of the coding regions indicate that carp Hsc70-1 and Hsc70-2 belong to the Hsp70 cognate subfamily. The expressions of the two hsc70 genes were followed by semi-quantitativ...

journal_title：Biochemical and biophysical research communications

authors： Ali KS,Dorgai L,Abrahám M,Hermesz E

更新日期：2003-08-01 00:00:00

abstract：:The metabolites of inflammatory cells produced by massive infection of the peritoneal cavity of two related European species of frogs, Rana temporaria and Rana arvalis were examined for lipoxygenase-generated products of exogenous arachidonic acid. Cells of Rana temporaria produced large amounts of 5-hydroxyeicosatetr...

journal_title：Biochemical and biophysical research communications

authors： Green FA,Herman CA,Herman RP,Claesson HE,Hamberg M

更新日期：1987-01-30 00:00:00

abstract：:VHL tumor suppressor protein contains two domains, alpha and beta. The alpha-domain is involved in the formation of a large protein complex suggested to be involved in ubiquitin-mediated protein degradation. However, the role of the beta-domain, which may recognize the target proteins for protein degradation, remains ...

journal_title：Biochemical and biophysical research communications

authors： Okuda H,Hirai S,Takaki Y,Kamada M,Baba M,Sakai N,Kishida T,Kaneko S,Yao M,Ohno S,Shuin T

更新日期：1999-09-24 00:00:00

abstract：:The present study revealed that low density lipoprotein (LDL) obtained from rabbits fed highly purified eicosapentaenoic acid-ethyl ester (EPA-E) (EPA-LDL) was significantly less susceptible to oxidative modification catalyzed by Cu2+ as compared to LDL from rabbits fed a normal diet (control LDL). In a comparison of ...

journal_title：Biochemical and biophysical research communications

authors： Saito H,Chang KJ,Tamura Y,Yoshida S

更新日期：1991-02-28 00:00:00

abstract：:In this report, we demonstrate that HL-60 nuclei isolated in calcium but not EGTA containing buffers specifically bind PE and express approximately 37,000 receptor sites/nucleus. Nuclear phorbol ester binding is lost by isolation in the absence of calcium, but can be repleted by the addition of partially purified prot...

journal_title：Biochemical and biophysical research communications

authors： Kraft AS,Appling C,Berkow RL

更新日期：1987-04-14 00:00:00

abstract：:The P-type ATPases affected in Menkes and Wilson diseases, ATP7A and ATP7B, respectively, are key copper transporters that regulate copper homeostasis. The N termini of these proteins are critical in regulating their function and activity, and contain six copper-binding motifs MxCxxC. In this study, we describe the id...

journal_title：Biochemical and biophysical research communications

authors： Lim CM,Cater MA,Mercer JF,La Fontaine S

更新日期：2006-09-22 00:00:00

abstract：:Chironomus ramosus is known to tolerate high doses of gamma radiation exposure. Larvae of this insect possess more than 95% of hemoglobin (Hb) in its circulatory hemolymph. This is a comparative study to see effect of gamma radiation on Hb of Chironomus and humans, two evolutionarily diverse organisms one having extra...

journal_title：Biochemical and biophysical research communications

authors： Gaikwad PS,Panicker L,Mohole M,Sawant S,Mukhopadhyaya R,Nath BB

更新日期：2016-08-05 00:00:00

abstract：:Human prostatic acid phosphatase (hPAP) is intensely expressed in epithelial cells of the prostate gland following puberty. Its regulatory regions were analyzed in transgenic mice and cell line transfections, in order to clarify the mechanisms of tissue-specific gene expression. A construct containing the sequence of ...

journal_title：Biochemical and biophysical research communications

authors： Shan J,Porvari K,Kivinen A,Patrikainen L,Halmekytö M,Jänne J,Vihko P

更新日期：2003-11-28 00:00:00

abstract：:Angiogenesis is critical to a wide range of physiological and pathological processes. Scutellarin, a major flavonoid of a Chinese herbal medicine Erigeron breviscapus (Vant.) Hand. Mazz. has been shown to offer beneficial effects on cardiovascular and cerebrovascular functions. However, scutellarin's effects on angiog...

journal_title：Biochemical and biophysical research communications

authors： Gao ZX,Huang DY,Li HX,Zhang LN,Lv YH,Cui HD,Zheng JH

更新日期：2010-09-10 00:00:00

abstract：:Cyclooxygenase-2 (COX-2) expression is increased by hypertonicity. Therefore we hypothesized that hypertonicity increased PGE(2) can modulate the sodium transporters (Na(+)/K(+)-ATPase: NKA, epithelial sodium channel: ENaC, and sodium hydrogen exchanger: NHE) in M1 cortical collecting duct (CCD) cells. We demonstrated...

journal_title：Biochemical and biophysical research communications

authors： Nasrallah R,Paris G,Hébert RL

更新日期：2012-02-10 00:00:00

abstract：:The conformation of lens crystallins in vivo or in a highly concentrated solution is not well established. Most studies were carried out in dilute solutions in which protein-protein interaction is minimal. In order to see whether there is conformational change (tertiary and secondary structures) when crystallin soluti...

journal_title：Biochemical and biophysical research communications

authors： Liang JJ,Chakrabarti B

更新日期：1998-05-19 00:00:00

abstract：:We previously showed that SIRT1 deacetylase inhibits proliferation of hepatocellular carcinoma cells expressing hepatitis B virus (HBV) X protein (HBX), by destabilization of β-catenin. Here, we report another role for SIRT1 in HBX-mediated resistance to oxidative stress. Ectopic expression and enhanced activity of SI...

journal_title：Biochemical and biophysical research communications

authors： Srisuttee R,Koh SS,Malilas W,Moon J,Cho IR,Jhun BH,Horio Y,Chung YH

更新日期：2012-12-07 00:00:00

abstract：:We have previously reported the establishment of preadipocyte cell lines, termed dedifferentiated fat (DFAT) cells, from mature adipocytes of various animals. DFAT cells possess long-term viability and can redifferentiate into adipocytes both in vivo and in vitro. Furthermore, DFAT cells can transdifferentiate into os...

journal_title：Biochemical and biophysical research communications

authors： Kazama T,Fujie M,Endo T,Kano K

更新日期：2008-12-19 00:00:00

abstract：:The crude extracts from Sphingomonas paucimobilus containing 2-hydroxy fatty acid-rich sphingolipids were found to oxidize [1-14C]-myristate to 2-hydroxymyristate in the presence of NADH. The myristate-oxidation activity was partially purified about 290-fold from the cell-free extracts by ammonium sulfate fractionatio...

journal_title：Biochemical and biophysical research communications

authors： Matsunaga I,Kusunose E,Yano I,Ichihara K

更新日期：1994-06-30 00:00:00