当前位置： SCI文献检索 > CRITICAL CARE MEDICINE期刊下所有文献 > Effects of a single-dose hypertonic saline hydroxyethyl starch on cerebral blood flow, long-term outcome, neurogenesis, and neuronal survival after cardiac arrest and cardiopulmonary resuscitation in rats*.

Effects of a single-dose hypertonic saline hydroxyethyl starch on cerebral blood flow, long-term outcome, neurogenesis, and neuronal survival after cardiac arrest and cardiopulmonary resuscitation in rats*.

Abstract：

OBJECTIVE:The beneficial effects of hypertonic saline on neuronal survival and on cerebral blood flow have been shown in several animal models of global and focal brain ischemia. Because of the potential benefits of hypertonic solutions, it is hypothesized that hydroxyethyl starch enhances cerebral blood flow and improves long-term outcome after cardiac arrest and cardiopulmonary resuscitation in an animal model. DESIGN:Laboratory animal study. SETTING:University animal research laboratory. SUBJECTS:Fifty-nine male Sprague-Dawley rats. INTERVENTIONS:Rats were randomized to receive either 7.2% saline/6% hypertonic saline hydroxyethyl starch (4 mL/kg) or vehicle (NaCl 0.9 %) after 9 mins of asphyxic cardiac arrest and cardiopulmonary resuscitation. Local cerebral blood flow and physiologic parameters were evaluated during arrest and early restoration of spontaneous circulation. Survival and neurologic assessment were evaluated over a 7-day observation period. Animals received 5-bromo-2-deoxyuridine for 6 days. Neuronal injury and neurogeneration (5-bromo-2-deoxyuridine positive neurons) were quantified on day 7 after cardiac arrest and cardiopulmonary resuscitation. MEASUREMENTS AND MAIN RESULTS:Hypertonic saline hydroxyethyl starch treatment resulted in an accentuated local cerebral blood flow during early reperfusion, compared to the vehicle group. Animal survival and neurologic outcome were not altered between groups. Neurohistopathological injury was present in hippocampal CA1 and neocortex with no effects of hypertonic saline hydroxyethyl starch on neuronal survival. Increased neurogeneration was found in the dentate gyrus after cardiac arrest/cardiopulmonary resuscitation, which was not influenced by hypertonic saline hydroxyethyl starch administration. CONCLUSIONS:Despite promising results in other models of brain injury, hypertonic saline hydroxyethyl starch failed to improve the outcome when administered after asphyxic cardiac arrest/cardiopulmonary resuscitation in rats. One major difference between the cardiac arrest/cardiopulmonary resuscitation model and other models of brain ischemia is that the effects of asphyxic cardiac arrest involve the whole organism (post-cardiac arrest syndrome) and not exclusively the brain leading to a more severe injury. This might explain why hypertonic saline hydroxyethyl starch has failed to improve outcome in the present model.

journal_name

Crit Care Med

journal_title

Critical care medicine

authors

Noppens RR,Kelm RF,Lindemann R,Engelhard K,Werner C,Kempski O

doi

10.1097/CCM.0b013e31824e6750

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

2149-56

issue

eissn

0090-3493

issn

1530-0293

journal_volume

pub_type

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Effects of a single-dose hypertonic saline hydroxyethyl starch on cerebral blood flow, long-term outcome, neurogenesis, and neuronal survival after cardiac arrest and cardiopulmonary resuscitation in rats*.