Abstract:
:The aim of this study was to determine whether the growth and differentiation of limbal epithelial stem cell cultures could be controlled through manipulation of the oxygen tension. Limbal epithelial cells were isolated from corneoscleral disks, and cultured using either feeder cells in a growth medium supplemented with serum (3T3 system) or without feeder cells in a dedicated serum-free medium (EpiLife). During the culture, the cells were maintained either at ambient oxygen tension (20%) or at different levels of hypoxia (15, 10, 5, and 2% oxygen). The effect of oxygen on cell growth, progression through cell cycle, colony forming efficiency (CFE), and expression of stem cell (ABCG2 and p63α) and differentiation (CK3) markers was determined throughout the culture period of up to 18 days. Low oxygen levels favored a stem cell phenotype with a lower proliferative rate, high CFE, and a relatively higher expression of ABCG2 and p63α, while higher levels of oxygen led not only to decreased CFE but also to increased proportion of differentiated cells positive for CK3. Hypoxic cultures may thus potentially improve stem cell grafts for cultured limbal epithelial transplantation (CLET).
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Bath C,Yang S,Muttuvelu D,Fink T,Emmersen J,Vorum H,Hjortdal J,Zachar Vdoi
10.1016/j.scr.2013.01.004subject
Has Abstractpub_date
2013-05-01 00:00:00pages
349-60issue
3eissn
1873-5061issn
1876-7753pii
S1873-5061(13)00011-1journal_volume
10pub_type
杂志文章abstract::Thrombocytopenia 2 (THC2) is a major type of inherited thrombocytopenia caused by the persistent ANKRD26 expression during the late stage of megakaryocytopoiesis. For the first time, we generated a human induced pluripotent stem cell (hiPSC) line SHAMUi001-A from the bone marrow hematopoietic progenitor cells of a THC...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.102002
更新日期:2020-10-01 00:00:00
abstract::Neural progenitor cells have been proposed as a therapy for central nervous system disorders, including neurodegenerative diseases and trauma injuries, however their accessibility is a major limitation. We recently isolated Tuj1+ cells from skeletal muscle culture of Nestin-GFP transgenic mice however whether they for...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2012.09.003
更新日期:2013-01-01 00:00:00
abstract::Rabbit induced pluripotent stem cells (rbiPSCs) possess the characteristic features of primed pluripotency as defined in rodents and primates. In the present study, we reprogrammed rbiPSCs using human Krüppel-like factors (KLFs) 2 and 4 and cultured them in a medium supplemented with fetal calf serum and leukemia inhi...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2017.09.001
更新日期:2017-10-01 00:00:00
abstract::GADD45A is a DNA damage and stressful growth arrest inducible protein, also it is shown to a be tumor suppressor gene and a chromatin relaxer associated with opening chromatin during the somatic reprogramming. However, its role in human embryonic stem cells and human embryonic stem cell modeled development has been me...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.102090
更新日期:2020-12-01 00:00:00
abstract::Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying two mutations in plakophilin-2 (PKP2) gene using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. Pluripotency was confirmed i...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2017.08.014
更新日期:2017-10-01 00:00:00
abstract::Transplantation of primary hepatocytes has been used in treatments for various liver pathologies and end-stage liver disease. However, shortage of donor tissue and the inability of hepatocyte proliferation in vitro have lead to alternative methods such as stem cell-derived hepatocyte-like cells (HLCs). Mesenchymal str...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101471
更新日期:2019-07-01 00:00:00
abstract::Spinocerebellar ataxia type-12 (SCA12) is a neurological disorder caused due to triplet (CAG) repeat expansion in 5' UTR of PPP2R2B. It is one of the most prominent SCA-subtype in Indian population and till date no patient specific models have been described. Human-induced-pluripotent-stem cell (HiPSC) based disease m...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.08.008
更新日期:2018-08-01 00:00:00
abstract::By means of retroviral transduction using the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC primary human amniotic fluid cells (AFCs) were reprogrammed into several iPSC lines. Pluripotency was confirmed both in vitro and in vivo. A comparative transcriptome analysis of the AF-derived iPSC line 41 and the human emb...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2015.11.003
更新日期:2015-11-01 00:00:00
abstract::We report here a transgenic murine induced pluripotent stem cell (iPSC) line expressing puromycin N-acetyltransferase (PAC) and enhanced green fluorescent protein (EGFP) under the control of α-myosin heavy chain promoter. This transgenic cell line reproducibly differentiates into EGFP-expressing cardiomyocytes (CMs) w...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.07.007
更新日期:2016-09-01 00:00:00
abstract::Human induced pluripotent stem cell-lines (iPSCs) of Indian origin NCCSi005A and NCCSi006A were established by reprogramming of CD4+T cells, isolated from the peripheral blood mononuclear cells (PBMCs) of two healthy female donors. Reprogramming was achieved using integration free, Sendai viral vector system expressin...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101506
更新日期:2019-08-01 00:00:00
abstract::Pathological myopia (PM) is a retinal degenerative disease with an increasing prevalence in Asia. The peripheral blood mononuclear cells (PBMCs) from a patient with PM were successfully reprogrammed to induced pluripotent stem cells (iPSCs) using integration-free method, Sendai viral (SeV) vectors expressing OCT4, SOX...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.101369
更新日期:2019-01-01 00:00:00
abstract::Our work aimed at evaluating the role of adipose stem cells (ASC) on chondrocytes from osteoarthritic (OA) patients and identifying the mediators involved. We used primary chondrocytes, ASCs from different sources and bone marrow mesenchymal stromal cells (MSC) from OA donors. ASCs or MSCs were co-cultured with chondr...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.05.008
更新日期:2013-09-01 00:00:00
abstract::The Genea079 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying compound heterozygous mutations in the NEB gene, exon 55 deletion & c.15110dupA, indicative of Nemaline Myopathy Type 2 (NEM2). Following ICM outgrowth on inactivated human feeders, karyotype w...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.02.015
更新日期:2016-03-01 00:00:00
abstract::Derivation of induced pluripotent stem (iPS) cells is mainly an epigenetic reprogramming process. It is still quite controversial how genomic imprinting is reprogrammed in iPS cells. Thus, we derived multiple iPS clones from genetically identical mouse somatic cells. We found that parentally inherited imprint was vari...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.05.011
更新日期:2013-09-01 00:00:00
abstract::The future application of human embryonic stem cells (hESC) for therapeutic approaches requires the development of xeno-free culture conditions to prevent the potential transmission of animal pathogens or xenobiotic substances to hESC. An important component of the majority of hESC culture systems developed is the req...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2010.08.002
更新日期:2011-01-01 00:00:00
abstract::Cell-mediated gene therapy is a possible means to treat muscular dystrophies like Duchenne muscular dystrophy. Autologous patient stem cells can be genetically-corrected and transplanted back into the patient, without causing immunorejection problems. Regenerated muscle fibres derived from these cells will express the...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.05.004
更新日期:2018-07-01 00:00:00
abstract::It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120 days with modified Oct4 along with Sox2, Klf4, ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2015.08.005
更新日期:2015-09-01 00:00:00
abstract::Recent clinical trials show the efficacy of Adipose-derived Stromal Cells (ASCs) in contrasting the osteoarthritis scenario. Since it is quite accepted that ASCs act predominantly through a paracrine mechanism, their secretome may represent a valid therapeutic substitute. The aim of this study was to investigate the e...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101463
更新日期:2019-07-01 00:00:00
abstract::Crumbs homologue 1 (CRB1) mutations have been found in retinitis pigmentosa (RP) patients lead to severe retinal dystrophies. The human induced pluripotent stem (iPS) cell line CSUASOi003-A derived from peripheral blood mononuclear cells (PBMCs) of a patient carrying two heterozygous mutations (2249G>A p.G750D and c.2...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.101742
更新日期:2020-04-01 00:00:00
abstract::Haploidentical hematopoietic stem-cell transplantation (haplo-HSCT) is associated with an increased risk of graft failure and severe graft-versus-host disease (GVHD). Mesenchymal stromal cells (MSCs) have been shown to support in vivo normal hematopoiesis and to display potent immunesuppressive effects. We cotransplan...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.10.001
更新日期:2014-01-01 00:00:00
abstract::Human teratoma is a germ cell tumor that contains normal tissues (e.g., hair, skin or cartilage) differentiated from embryonal germ layers. Because of the feature of this tumor, we hypothesized that human teratomas contain multipotent stem cells that can develop into various non-cancerous normal tissues. In this study...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101633
更新日期:2019-12-01 00:00:00
abstract::Leucine rich repeat kinase (LRRK2) is the most prevalent genetic cause for Parkinson's disease. LRRK2 p.G2385R is an Asian specific genetic risk factor for sporadic Parkinson's disease. We generated two induced pluripotent stem cells (iPSCs), IBMS-iPSC-018-09 and IBMS-iPSC-020-01, from the peripheral blood mononuclear...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.01.034
更新日期:2018-04-01 00:00:00
abstract::To develop cell therapies for damaged nervous tissue with human neural stem/progenitor cells (hNPCs), the risk of an immune response and graft rejection must be considered. There are conflicting results and lack of knowledge concerning the immunocompetence of hNPCs of different origin. Here, we studied the immunogenic...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.01.001
更新日期:2013-05-01 00:00:00
abstract::Leber's hereditary optic neuropathy (LHON) is a maternally inherited mitochondrial disease caused by homoplasmic point mutations in complex I subunit genes of mitochondrial DNA. In this report, we generated an induced pluripotent stem cell (iPSCs) line, TVGH-iPSC-010-09, from the peripheral blood mononuclear cells of ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.01.029
更新日期:2018-04-01 00:00:00
abstract::Aside from its role in cell membrane integrity, cholesterol is a key component in steroid hormone production. The vital functions of steroid hormones such as estrogen, testosterone, glucocorticoids (Gcrts) and mineralocorticoids (Mnrts) in perinatal and adult life are well understood; however, their role during early ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2015.04.010
更新日期:2015-07-01 00:00:00
abstract::Hematopoietic stem and progenitor cell (HSPC) transplantation is the paradigm for stem cell therapies. The protocol described here enables quantitative assessment of the body-wide HSPC reconstitution of different mature hematopoietic cells in mice based on their presence in circulating blood. The method determines don...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.102145
更新日期:2020-12-29 00:00:00
abstract::The niche concept of stem cell biology proposes a functional unit between the precursor cells and their local microenvironment, to which several cell types might contribute by cell-cell contacts, extracellular matrix, and humoral factors. We here established three co-culture models (with cell types separated by membra...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2015.09.010
更新日期:2015-11-01 00:00:00
abstract::Asparagine synthetase (ASNS) deficiency (ASNSD; MIM #615574) is a rare neurodevelopmental disorder caused by mutations in the ASNS gene. The ASNS gene maps to cytogenetic band 7q21.3 and is 35 kb long. ASNSD is characterised by congenital microcephaly, severely delayed psychomotor development, seizures, and hyperekple...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101583
更新日期:2019-12-01 00:00:00
abstract::Here we utilized the chromatin in vivo assay (CiA) mouse platform to directly examine the epigenetic barriers impeding the activation of the CiA:Oct4 allele in mouse embryonic fibroblasts (MEF)s when stimulated with a transcription factor. The CiA:Oct4 allele contains an engineered EGFP reporter replacing one copy of ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101470
更新日期:2019-07-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) isolated from the peripheral blood of a five months-old boy with glycogen storage disease type II(GSD II, also known as Pompe disease, PD) carries compound mutations R608X E888X in GAA gene. PBMCs were reprogrammed us...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101621
更新日期:2019-12-01 00:00:00