Abstract：
OBJECTIVE:The aim of this study was to investigate the antibiofilm activity of coumarin against Candida albicans. METHODS:The efficacy of coumarin against biofilm formation and the mature biofilm of C. albicans was quantified by crystal violet (CV) staining and the 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay. The effect of coumarin on C. albicans adhesion was assessed on polystyrene plates and by using the cell surface hydrophobicity (CSH) assay. The morphological transition of C. albicans was conducted in two types of hyphae-inducing media at 37°C. The expression of hypha/biofilm-related genes was evaluated using qRT-PCR analysis. A rescue experiment involving addition of exogenous cyclic adenosine monophosphate (cAMP) was performed to investigate the involvement of cAMP in the yeast-to-hyphae transition. A C. albicans-infected Caenorhabditis elegans model was used to test the anti-virulence efficacy of coumarin. RESULTS:Treatment with coumarin strongly affected the capacity of C. albicans to form biofilm and significantly impaired the preformed mature biofilm. The addition of coumarin notably inhibited C. albicans adhesion, CSH, and filamentation. The expression of some adhesion- and hypha-related genes, including HWP1, HYR1, ECE1, and ALS3, was remarkably down-regulated upon exposure to coumarin. Supplementation with cAMP partly rescued the coumarin-induced defects in hyphal development. Finally, coumarin prolonged survival in C. albicans-infected nematodes. CONCLUSION:Coumarin inhibited C. albicans biofilm, which was associated with attenuated adhesion and hyphal growth.
journal_name
J Mycol Medjournal_title
Journal de mycologie medicaleauthors
Xu K,Wang JL,Chu MP,Jia Cdoi
10.1016/j.mycmed.2018.12.003subject
Has Abstractpub_date
2019-04-01 00:00:00pages
28-34issue
1eissn
1156-5233issn
1773-0449pii
S1156-5233(18)30176-8journal_volume
29pub_type
杂志文章abstract：:Symptomatic candiduria often occurs in patients with indwelling bladder catheters or immunocompromised host. Isolation of Candida in urine in high-risk patients should primarily be considered as a marker for candidemia. Hematological and genitourinary malignancies are one of the main risk factors associated with Candi...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章,评审
doi：10.1016/j.mycmed.2017.01.006
更新日期：2017-06-01 00:00:00
abstract：:Tricyclazole as a common fungicide wildly used to control rice blast disease in the Asian country may induce azole resistance in Aspergillus fumigatus isolates. The main reason of the acquired azole resistance is probably environmental exposure through wide fungicide use in agriculture. The present study was conducted...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2017.12.007
更新日期：2018-03-01 00:00:00
abstract：INTRODUCTION:In Mauritania, only an investigation carried out 26 years ago allows to report epidemiological findings about tinea capitis in Nouakchott. The objective of this study was to reactualize the tinea capitis epidemiological, clinical and mycological profile in school children and to compare this profile in urb...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2020.101048
更新日期：2020-10-13 00:00:00
abstract：:The growing trends of emergence of antifungal-resistant Candida strains has recently been inspired the researchers to design new antifungal agents with novel mechanisms of action. Glabridin is an originally natural substrate with multiple biological activities which propose it as a novel anticancer, antimicrobial and ...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2017.05.002
更新日期：2017-09-01 00:00:00
abstract：:Cryptococcosis is a potentially fatal fungal disease caused by the basidiomycetes yeasts Cryptococcus neoformans and C. gattii with high predilection to invade the central nervous system mainly in immunocompromised hosts. Skin can be secondarily involved in disseminated infection or be exceptionally involved as primar...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2017.04.104
更新日期：2017-09-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Vulvovaginal candidiasis (VVC) is the most prevalent vaginitis in women, accounting for 10 million medical referrals a year. Vaginal clotrimazole is a drug of choice for VVC treatment. However, increased drug resistance to this microorganism has led to an interest in naturally derived antifunga...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.mycmed.2017.07.003
更新日期：2017-12-01 00:00:00
abstract：OBJECTIVE:In this paper, we describe the preparation and synthesis of several enynediesters, conjugated diynes and acetylenic compounds from starting materials comprising one hydroxyl and one acetylenyl group and their antifungal activity. MATERIALS AND METHODS:For synthesis of the compounds, a combined solution of N,...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2012.06.001
更新日期：2012-09-01 00:00:00
abstract：:We present the case of an actinomycotic mycetoma of the foot due to Actinomycetes viscosus. It evolved for nine years on the foot of a 26-year-old patient from a rural environment: Douar Inezgane (city in southern Morocco). Bacteriological study of the skin and grains confirmed the diagnosis. It showed positive bacill...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2014.10.025
更新日期：2015-03-01 00:00:00
abstract：OBJECTIVE:The aim of this study was the detection of Aspergillus species and Mycobacterium tuberculosis together in bronchoalveolar lavage (BAL) using of multiplex PCR. MATERIAL AND METHODS:In this study, from September 2012 until June 2013, 100 bronchoalveolar lavage (BAL) specimens were collected from patients suspe...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2015.02.041
更新日期：2015-06-01 00:00:00
abstract：:Dermatophytes are responsible for widespread superficial fungal infections, currently representing a real public health problem. Some of the fungi involved in these mycoses are transmitted by pets, illustrating great host specificity within this fungal group. Thus, a new variety of zoophilic dermatophyte has been desc...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2016.05.005
更新日期：2016-09-01 00:00:00
abstract：:Candida spp. vertebral osteomyelitis is rare. Clinical presentation is unspecific. Diagnosis requires mycological culture of a biopsy specimen. Therapeutic management is based on prolonged course of azole or liposomal amphotericin B. We report the case of Candida tropicalis vertebral osteomyelitis with epidural involv...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2017.09.005
更新日期：2017-12-01 00:00:00
abstract：:Fusarium onychomycosis is not uncommon in tropical countries but is worth reporting. We report a case of nondermatophytic onychomycosis by Fusarium oxysporum in an immunocompetent woman from Buldhana district of Maharashtra (India). Bilateral involvement of great toe nail, chronic duration and acquisition of infection...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2015.12.003
更新日期：2016-03-01 00:00:00
abstract：BACKGROUND:Candidemia is a nosocomial infection of increasing importance, associated with high morbidity and mortality. The aim of this study is to describe the species distribution, risk factors, management and outcomes of patients with candidemia. METHODS:We conducted a retrospective study at Centro Hospitalar Unive...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2019.08.002
更新日期：2019-12-01 00:00:00
abstract：:One hundred and twenty-five samples were collected from eight different sites in the vicinity of Sanjay Gandhi National Park (SGNP) and screened for the presence of keratinophilic fungi using hair baiting technique for isolation. Seventy-three isolates were recovered and identified. The cultures were identified using ...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2014.08.004
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVE:To determine the fungal species isolated from skin lesions of different animals suspected of having dermatomycoses and their prevalence in different regions of Iran. MATERIALS AND METHODS:A total of 1011 animals (292 dogs, 229 cats, 168 horses, 100 camels, 98 cows, 60 squirrels, 37 birds, 15 sheep, 6 goats, ...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2016.04.007
更新日期：2016-06-01 00:00:00
abstract：:Candida nivariensis is a cryptic fungal species classified within the Candida glabrata complex. It was described for the first time in 2005 by the means of DNA sequencing. We report a rare case of C. nivariensis deep-seated infection occurring in a 77-year-old man hospitalized for cysto-prostatectomy. Phenotypic testi...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章,评审
doi：10.1016/j.mycmed.2020.101042
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVE:This study was carried out to show possible similarities between discoid lupus erythematosus clinical signs and histopathological findings and dermatophytosis. PATIENT AND METHODS:A 43-year-old woman with diffused erythematous scaling lesions on her neck was studied. Skin biopsy was performed using H&E and P...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2011.12.001
更新日期：2012-03-01 00:00:00
abstract：:Tineabarbae is a rare form of dermatophytosis that affects hair follicles of the beard and moustache. Dermoscopy could prove useful to identify parasitism of hair of the beard, just as it has proven useful in the diagnosis of Tineacapitis. We present the first fully documented case series of T. barbae with clinical, d...
journal_title：Journal de mycologie medicale
pub_type：
doi：10.1016/j.mycmed.2020.101104
更新日期：2020-12-15 00:00:00
abstract：INTRODUCTION:Definition, diagnosis and treatment of allergic fungal sinusitis remain difficult and controversial despite the increasing number of publications. We report a case of an allergic fungal sinusitis with a review of the literature. CLINICAL CASE:A 45-year-old man consulted for sinusal polyposis resistant to ...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章,评审
doi：10.1016/j.mycmed.2015.09.007
更新日期：2015-12-01 00:00:00
abstract：:The research concerns algae of the genus Prototheca. They are found in the natural environment and they can cause a disease in animals and humans called protothecosis. The aim of the study was to evaluate the in vitro activity of the fruit and vegetable rinse agent SunSmile® Fruit & Vegetable Rinse (Sunrider Internati...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2018.03.008
更新日期：2018-06-01 00:00:00
abstract：INTRODUCTION:Otomycosis, a superficial fungal infection of the external auditory canal (EAC), is a disease with exceptionally high prevalence. AIM:The aim of this study was to determine the prevalence of otomycosis, the distribution of causative species and to evaluate epidemiological characteristics of these infectio...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2020.101011
更新日期：2020-09-01 00:00:00
abstract：INTRODUCTION:Candida albicans is the most prevalent fungal pathogen in humans. Due to the development of drug resistance, there is today a need for new antifungal agents for the efficient management of C. albicans infections. Therefore, we reviewed antifungal activity, mechanisms of action, possible synergism with anti...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章,评审
doi：10.1016/j.mycmed.2016.10.002
更新日期：2017-03-01 00:00:00
abstract：OBJECTIVE:The increasing importance of dermatophytoses and emerging resistance of dermatophytes to current synthetic antifungals have stimulated the search for safer and more effective alternative drugs from natural sources. The present study was carried out to identify antagonistic bacteria of soil origin with strong ...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2014.01.117
更新日期：2014-06-01 00:00:00
abstract：:Curvularia lunata is a dematiaceous fungus that rarely causes cutaneous infections in immunocompetent humans. We describe a case of mycetoma pedis caused by Curvularia lunata in a 50-year-old woman from a rural farming community who had prolonged morbidity due to diagnostic delay. She improved with antifungal therapy,...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2013.09.005
更新日期：2014-06-01 00:00:00
abstract：:Human and animal mycoses become more frequent and more resistant to traditional treatments. In this work, we tested the in vitro antifungal activity of acetonic extracts of Punica granatum L., Quercus suber L. and Vicia faba L. against seven pathogen fungi and the in vivo antifungal activity against Candida albicans a...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2016.10.004
更新日期：2017-03-01 00:00:00
abstract：:In Algeria, many bacterial biofilms have been studied but those of fungal origin, particularly those due to the yeast Candida albicans remained unidentified. The present study was performed at the Chabane Hamdoune hospital in Maghnia (Algeria), where 51 strains of C. albicans representing 16.94% of all taken samples w...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2011.07.006
更新日期：2011-09-01 00:00:00
abstract：:Mucormycosis is a progressive, opportunistic fungal infection with a high risk of mortality. Also mucormycosis may attack any organ system and may be accompanied by hemodynamic instability and difficult airway management. This study aimed to evaluate the anesthetic management of patients undergoing surgical resection ...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2019.07.001
更新日期：2019-09-01 00:00:00
abstract：OBJECTIVE:Dermatophytes are taxonomically classified in the genera Trichophyton, Microsporum, and Epidermophyton. Pleomorphism, cultural variability, slow growth and sporulation, and the need for additional physiological tests make dermatophytes notoriously difficult to identify. The present study aimed to compare the ...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2014.10.022
更新日期：2015-03-01 00:00:00
abstract：OBJECTIVE OF THE STUDY:Candidiasis and dermatophytoses are benign infections in humans and animals, but they are very dreaded diseases in immunocompromised individuals. These infections become resistant to different treatments which make them more dangerous. In this work, we tried to find a new way for treating them. S...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2018.06.003
更新日期：2018-09-01 00:00:00
abstract：:To study invasive pulmonary Aspergillosis (IPA), we depleted neutrophils in mice using the monoclonal antibody anti-Gr-1/Ly-6G. Immunocompetent and neutropenic mice were infected via intratracheal with conidia of Aspergillus fumigatus clinical isolates, characterized as either higher or lower elastase producers. Neutr...
journal_title：Journal de mycologie medicale
pub_type： 杂志文章
doi：10.1016/j.mycmed.2018.10.003
更新日期：2019-06-01 00:00:00
