当前位置： SCI文献检索 > JOURNAL DE MYCOLOGIE MEDICALE期刊下所有文献 > A multiplex PCR method for detection of Aspergillus spp. and Mycobacterium tuberculosis in BAL specimens.

A multiplex PCR method for detection of Aspergillus spp. and Mycobacterium tuberculosis in BAL specimens.

Abstract：

OBJECTIVE:The aim of this study was the detection of Aspergillus species and Mycobacterium tuberculosis together in bronchoalveolar lavage (BAL) using of multiplex PCR. MATERIAL AND METHODS:In this study, from September 2012 until June 2013, 100 bronchoalveolar lavage (BAL) specimens were collected from patients suspected of tuberculosis (TB). After the direct and culture test, multiplex PCR were utilized in order to diagnose Aspergillus species and M. tuberculosis. Phenol-chloroform manual method was used in order to extract DNA from these microorganisms. Aspergillus specific primers, M. tuberculosis designed primers and beta actin primers were used for multiplex PCR. RESULTS:In this study, by multiplex PCR method, Aspergillus species were identified in 12 samples (12%), positive samples in direct and culture test were respectively 11% and 10%. Sensitivity and specificity of this method in comparison to direct test were respectively 100% and 98.8%, also sensitivity and specificity of this method in comparison to culture test were respectively 100% and 97.7%. In this assay, M. tuberculosis was identified in 8 samples (8%). Mycobacterium-positive samples in molecular method, direct and culture test were respectively 6%, 5% and 7%. Sensitivity and specificity of PCR method in comparison to direct test were 80% and 97.8% also sensitivity and specificity of this method in comparison to culture test was 71.4% and 98.9%. CONCLUSION:In the present study, multiplex PCR method had higher sensitivity than direct and culture test in order to identify and detect Aspergillus, also this method had lower sensitivity for identification of M. tuberculosis, suggesting that the method of DNA extraction was not suitable.

journal_name

J Mycol Med

journal_title

Journal de mycologie medicale

authors

Amini F,Kachuei R,Noorbakhsh F,Imani Fooladi AA

doi

10.1016/j.mycmed.2015.02.041

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

e59-64

issue

2

eissn

1156-5233

issn

1773-0449

pii

S1156-5233(15)00046-3

journal_volume

25

pub_type

杂志文章

相关文献

JOURNAL DE MYCOLOGIE MEDICALE文献大全
  • The cellular and molecular defense mechanisms of the Candida yeasts against azole antifungal drugs.

    abstract：:The molecular mechanisms supporting resistance to azole antifungals have attracted a great interest during the last decades because of the emergence of clinical resistance to the treatment of fungal infections. The availability of genome sequencing data, of molecular biology tools, and of a large set of clinical and l...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章,评审

    doi：10.1016/j.mycmed.2012.04.004

    authors： Noël T

    更新日期：2012-06-01 00:00:00

  • [Detection of Aspergillus antigen galactomannan using ELISA method: validation of the performances of the method for accreditation].

    abstract：:Diagnosis of invasive aspergillosis for patients with high risk of infection is based on the monitoring of Aspergillus antigenemia assessed by the detection of galactomannan in serum by a sandwich-type ELISA (Biorad(®)). The validation of the method was displayed according to the guide COFRAC SH GTA 04. The internal q...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2012.12.048

    authors： Kauffmann-Lacroix C,Arvier M,Charron M,Rodier MH,Vassault A

    更新日期：2013-03-01 00:00:00

  • [Internal and external quality controls for Elisa techniques of aspergillosis serodiagnosis: proposals of the group "sérodiagnostic fongique" of the Société française de mycologie médicale].

    abstract：:In the end of May 2012, a meeting of the group "sérodiagnostic fongique" of the "Société française de mycologie médicale" had concerned quality controls to use, in particular, in the follow-up of Elisa techniques. A preliminary investigation showed that the internal quality controls (CIQ), according to the terms defin...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2012.11.001

    authors： Persat F,Lachaud L,Rabérin H,Poggi B,Roques C,Gangneux JP,les Participants du GroupeSérodiagnostic Fongique de la Société Française de Mycologie Médicale.

    更新日期：2013-03-01 00:00:00

  • Species distribution patterns and epidemiological characteristics of otomycosis in Southeastern Serbia.

    abstract：INTRODUCTION:Otomycosis, a superficial fungal infection of the external auditory canal (EAC), is a disease with exceptionally high prevalence. AIM:The aim of this study was to determine the prevalence of otomycosis, the distribution of causative species and to evaluate epidemiological characteristics of these infectio...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2020.101011

    authors： Tasić-Otašević S,Golubović M,Đenić S,Ignjatović A,Stalević M,Momčilović S,Bojanović M,Arsić-Arsenijević V

    更新日期：2020-09-01 00:00:00

  • Antifungal activity of a soil isolate of Pseudomonas chlororaphis against medically important dermatophytes and identification of a phenazine-like compound as its bioactive metabolite.

    abstract：OBJECTIVE:The increasing importance of dermatophytoses and emerging resistance of dermatophytes to current synthetic antifungals have stimulated the search for safer and more effective alternative drugs from natural sources. The present study was carried out to identify antagonistic bacteria of soil origin with strong ...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2014.01.117

    authors： Ranjbariyan A,Shams-Ghahfarokhi M,Razzaghi-Abyaneh M

    更新日期：2014-06-01 00:00:00

  • Fungemia in the French department of Mayotte, Indian Ocean: A 10 years survey.

    abstract：OBJECTIVE:This study aimed at providing original data on fungemia in the Centre Hospitalier de Mayotte in terms of prevalence, epidemiological characteristics of infected patients, yeast species distribution and profile of in vitro antifungals susceptibility. METHODS:A total of 223 positive blood cultures for yeasts w...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2020.101081

    authors： Wankap R,Mogo C,Niang M,Diallo A,Balloy L,Baes L,Collet L,Benoit-Cattin T,Permal S,Guegan H,Gangneux JP

    更新日期：2020-11-18 00:00:00

  • Mucormycosis outbreak due to Rhizopus microsporus after arthroscopic anterior cruciate ligament reconstruction surgery evaluated by RAPD and MALDI-TOF Mass spectrometry.

    abstract：BACKGROUND:Rhizopus microsporus is one of the main causative agents of mucormycosis. These mycoses are mostly described as isolated cases involving uncontrolled diabetes mellitus or immunosuppressed patients. In this work we report a nosocomial outbreak of mucormycosis due to R. microsporum involving three young immuno...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2018.09.002

    authors： Gamarra S,Chaves MS,Cabeza MS,Macedo D,Leonardelli F,Franco D,Boleas M,Garcia-Effron G

    更新日期：2018-12-01 00:00:00

  • Comparison of vaginal ointment of honey and clotrimazole for treatment of vulvovaginal candidiasis: A random clinical trial.

    abstract：BACKGROUND AND OBJECTIVE:Vulvovaginal candidiasis (VVC) is the most prevalent vaginitis in women, accounting for 10 million medical referrals a year. Vaginal clotrimazole is a drug of choice for VVC treatment. However, increased drug resistance to this microorganism has led to an interest in naturally derived antifunga...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.mycmed.2017.07.003

    authors： Banaeian S,Sereshti M,Rafieian M,Farahbod F,Kheiri S

    更新日期：2017-12-01 00:00:00

  • Carbohydrate hitched imidazoles as agents for the disruption of fungal cell membrane.

    abstract：:The fungal diseases represent an increasing global health burden and have transformed from a rare curiosity to the leading cause of human mortality. The present manuscript reports the antifungal potential of two novel compounds possessing a carbohydrate and an imidazole moiety. Antifungal susceptibility test determine...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2019.100910

    authors： Deswal D,Shukla P,Azad CS,Narula AK

    更新日期：2020-04-01 00:00:00

  • The in vitro efficacy of SunSmile® Fruit & Vegetable Rinse against pathogenic strains of Prototheca algae that cause mastitis in cows.

    abstract：:The research concerns algae of the genus Prototheca. They are found in the natural environment and they can cause a disease in animals and humans called protothecosis. The aim of the study was to evaluate the in vitro activity of the fruit and vegetable rinse agent SunSmile® Fruit & Vegetable Rinse (Sunrider Internati...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2018.03.008

    authors： Grzesiak B,Krukowski H,Głowacka A

    更新日期：2018-06-01 00:00:00

  • Murine model of invasive pulmonary Aspergillosis: Follow-up of tissue injury, fungal burden and mortality with distinct elastase production strains.

    abstract：:To study invasive pulmonary Aspergillosis (IPA), we depleted neutrophils in mice using the monoclonal antibody anti-Gr-1/Ly-6G. Immunocompetent and neutropenic mice were infected via intratracheal with conidia of Aspergillus fumigatus clinical isolates, characterized as either higher or lower elastase producers. Neutr...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2018.10.003

    authors： Silva RLH,Rosa-Milani E,Brunaldi MO,Maffei CML

    更新日期：2019-06-01 00:00:00

  • Activity of coumarin against Candida albicans biofilms.

    abstract：OBJECTIVE:The aim of this study was to investigate the antibiofilm activity of coumarin against Candida albicans. METHODS:The efficacy of coumarin against biofilm formation and the mature biofilm of C. albicans was quantified by crystal violet (CV) staining and the 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenyla...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2018.12.003

    authors： Xu K,Wang JL,Chu MP,Jia C

    更新日期：2019-04-01 00:00:00

  • Nondermatophytic onychomycosis by Fusarium oxysporum in an immunocompetent host.

    abstract：:Fusarium onychomycosis is not uncommon in tropical countries but is worth reporting. We report a case of nondermatophytic onychomycosis by Fusarium oxysporum in an immunocompetent woman from Buldhana district of Maharashtra (India). Bilateral involvement of great toe nail, chronic duration and acquisition of infection...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2015.12.003

    authors： Shah SR,Dalal BD,Modak MS

    更新日期：2016-03-01 00:00:00

  • [Study of the infectivity of Candida on peripheral vascular catheters collected from the University Hospital of Tlemcen].

    abstract：:Yeasts can adhere to medical implants and cause infections responsible for high morbidity and mortality among hospitalized patients. The objective of this study is to investigate the infectivity on peripheral vascular catheters collected from general surgery and cardiology in University Hospital of Tlemcen. The result...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章,评审

    doi：10.1016/j.mycmed.2017.10.002

    authors： Seghir A,Boucherit-Otmani Z,Boucherit K,Sari-Belkharroubi L

    更新日期：2017-12-01 00:00:00

  • Antifungal sensitivity and species of yeasts in oral mucosa of street mixed-breed dogs.

    abstract：:The aim of this research is to verify the yeast species isolated from oral mucosa in street mixed-breed dogs and to determine the antifungal profiles. After capturing and sedating the animals, oral mucosa samples were collected from fifty dogs and the materials were inoculated on Sabouraud dextrose agar with chloramph...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2020.101010

    authors： Navarro BS,Auler ME,Dos Santos RLO,da Silva Ruiz L,Nascimento DC,Felippe PAN,Domaneschi C,Moreira D,Baroni FA,Pires MFC,Paula CR

    更新日期：2020-12-01 00:00:00

  • [African histoplamosis. A report of three pediatric cases].

    abstract：:Histoplasmosis duboisii (Histoplasma capsulatum var duboisii) is uncommon disease especially in children. It is observed in Africa where the incidence is unknown. The authors report a series of three pediatric cases. The report concerned 2 girls and one boy who were 3, 9 and 4 year-old, respectively. Symptoms evolved ...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2017.01.013

    authors： Mabiala Babela JR,Mboutol Mandavo C,Nika Evrard R,Ossibi Ibara B,Lamah L,Ollandzobo Ikobo LC,Mouko A,Peko JF

    更新日期：2017-06-01 00:00:00

  • Anesthetic management of rhinoorbitocerebral mucormycosis; Focus on challenges.

    abstract：:Mucormycosis is a progressive, opportunistic fungal infection with a high risk of mortality. Also mucormycosis may attack any organ system and may be accompanied by hemodynamic instability and difficult airway management. This study aimed to evaluate the anesthetic management of patients undergoing surgical resection ...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2019.07.001

    authors： Karaaslan E

    更新日期：2019-09-01 00:00:00

  • Candida albicans biofilms formed into catheters and probes and their resistance to amphotericin B.

    abstract：:In Algeria, many bacterial biofilms have been studied but those of fungal origin, particularly those due to the yeast Candida albicans remained unidentified. The present study was performed at the Chabane Hamdoune hospital in Maghnia (Algeria), where 51 strains of C. albicans representing 16.94% of all taken samples w...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2011.07.006

    authors： Boucherit-Atmani Z,Seddiki SM,Boucherit K,Sari-Belkharoubi L,Kunkel D

    更新日期：2011-09-01 00:00:00

  • Anticandidal activity of silver nanoparticles synthesized using Streptomyces sp.VITPK1.

    abstract：OBJECTIVES:The aim of the present study was to evaluate the anticandidal activity of biologically synthesized silver nanoparticles using the culture filtrate of Streptomyces sp.VITPK1. MATERIALS AND METHODS:Silver nanoparticles were synthesized using the culture filtrate of Streptomyces species isolated from brine spr...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2014.03.004

    authors： Sanjenbam P,Gopal JV,Kannabiran K

    更新日期：2014-09-01 00:00:00

  • Vitamin D3 a new drug against Candida albicans.

    abstract：OBJECTIVE:In this study, we demonstrate that vitamin D3 had fungicidal activity against Candida albicans. MATERIAL AND METHODS:The susceptibility of the yeast strain to the vitamin D3 was investigated by the antimicrobial screening using modified agar diffusion method, minimum fungistatic concentrations (MFCs) and min...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2016.10.003

    authors： Bouzid D,Merzouki S,Bachiri M,Ailane SE,Zerroug MM

    更新日期：2017-03-01 00:00:00

  • Tinea capitis in school children from Mauritania: A comparative study between urban and rural areas.

    abstract：INTRODUCTION:In Mauritania, only an investigation carried out 26 years ago allows to report epidemiological findings about tinea capitis in Nouakchott. The objective of this study was to reactualize the tinea capitis epidemiological, clinical and mycological profile in school children and to compare this profile in urb...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2020.101048

    authors： Sy O,Diongue K,Ba O,Ahmed CB,Elbechir MA,Abdallahi MSM,Brahim M,Baidy BL,Ndiaye D

    更新日期：2020-10-13 00:00:00

  • In vitro activity of carvacrol, cinnamaldehyde and thymol combined with antifungals against Malassezia pachydermatis.

    abstract：:Malassezia pachydermatis is an important opportunistic agent of dermatitis and otitis in dogs. M. pachydermatis is generally treated with topical therapies using combinations of antifungal, antimicrobial and anti-inflammatory agents. We investigated the in vitro activities of carvacrol (CRV), cinnamaldehyde (CIN) and ...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2019.08.003

    authors： Schlemmer KB,Jesus FPK,Tondolo JSM,Weiblen C,Azevedo MI,Machado VS,Botton SA,Alves SH,Santurio JM

    更新日期：2019-12-01 00:00:00

  • [Actinomycosic mycetoma of the foot in Morocco due to Actinomycetes viscosus].

    abstract：:We present the case of an actinomycotic mycetoma of the foot due to Actinomycetes viscosus. It evolved for nine years on the foot of a 26-year-old patient from a rural environment: Douar Inezgane (city in southern Morocco). Bacteriological study of the skin and grains confirmed the diagnosis. It showed positive bacill...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2014.10.025

    authors： Baha H,Khadir K,Hali F,Benchikhi H,Zeghwagh A,Zerouali K,Belabbes H,El Mdaghri N,Soussi MA,Marnissi F,Kadioui F

    更新日期：2015-03-01 00:00:00

  • Comparative in vitro activities of seven antifungal drugs against clinical isolates of Candida parapsilosis complex.

    abstract：OBJECTIVE:Candida parapsilosis species complex, an important set of non-albicans Candida species, is known to cause candidaemia particularly in neonates and infants. However, the incidence has increased in recent years, owing to higher numbers of at individuals at risk for these infections. Our objective was to evaluat...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2020.100968

    authors： Khodavaisy S,Badali H,Meis JF,Modiri M,Mahmoudi S,Abtahi H,Salehi M,Dehghan Manshadi SA,Aala F,Agha Kuchak Afshari S,Lotfali E,Ahangarkani F,Rezaie S

    更新日期：2020-09-01 00:00:00

  • Isolation of keratinophilic fungi from selected soils of Sanjay Gandhi National Park, Mumbai (India).

    abstract：:One hundred and twenty-five samples were collected from eight different sites in the vicinity of Sanjay Gandhi National Park (SGNP) and screened for the presence of keratinophilic fungi using hair baiting technique for isolation. Seventy-three isolates were recovered and identified. The cultures were identified using ...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2014.08.004

    authors： Deshmukh SK,Verekar SA

    更新日期：2014-12-01 00:00:00

  • Glabridin triggers over-expression of MCA1 and NUC1 genes in Candida glabrata: Is it an apoptosis inducer?

    abstract：:The growing trends of emergence of antifungal-resistant Candida strains has recently been inspired the researchers to design new antifungal agents with novel mechanisms of action. Glabridin is an originally natural substrate with multiple biological activities which propose it as a novel anticancer, antimicrobial and ...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2017.05.002

    authors： Moazeni M,Hedayati MT,Nabili M,Mousavi SJ,Abdollahi Gohar A,Gholami S

    更新日期：2017-09-01 00:00:00

  • [Multifocal histoplasmosis due to Histoplasma capsulatum var. duboisii in a 22 year-old Senegalese patient without proven immunodepression].

    abstract：:The authors report a case of multifocal African histoplasmosis with polymorphic skin involvement occuring in a patient without proven immunosuppression. He was a young 22-year-old patient from eastern Senegal - but born in Ouganda - who presented with polymorphic skin lesions: ombilicated papulo-nodules, gums and ulce...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2016.03.004

    authors： Diadie S,Diatta B,Ndiaye M,Gaye M,Sow D,Ndiaye MT,Seck B,Diallo S,Diop A,Diallo M,Ly F,Niang SO,Kane A,Dieng MT

    更新日期：2016-09-01 00:00:00

  • Correlation between dermoscopy and direct microscopy in Tineabarbae.

    abstract：:Tineabarbae is a rare form of dermatophytosis that affects hair follicles of the beard and moustache. Dermoscopy could prove useful to identify parasitism of hair of the beard, just as it has proven useful in the diagnosis of Tineacapitis. We present the first fully documented case series of T. barbae with clinical, d...

    journal_title：Journal de mycologie medicale

    pub_type：

    doi：10.1016/j.mycmed.2020.101104

    authors： Gómez-Moyano E,Martínez Pilar L,Vera Casaño A,Martín González T,Moyano Almagro B,Fernandez Ballesteros MD,Martínez Garcia S,Godoy Diaz DJ

    更新日期：2020-12-15 00:00:00

  • In vitro susceptibility of Candida spp. to fluconazole, itraconazole and voriconazole and the correlation between triazoles susceptibility: Results from a five-year study.

    abstract：:Candida spp. is a common cause of invasive fungal disease. The aim of this study was to examine the susceptibility of Candida spp. to fluconazole, itraconazole and voriconazole and explore the correlation between triazoles susceptibility. The antifungal susceptibility in the present study was measured by ATB Fungus 3 ...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2018.03.005

    authors： Lei J,Xu J,Wang T

    更新日期：2018-06-01 00:00:00

  • A comparative study on morphological versus molecular identification of dermatophyte isolates.

    abstract：OBJECTIVE:Dermatophytes are taxonomically classified in the genera Trichophyton, Microsporum, and Epidermophyton. Pleomorphism, cultural variability, slow growth and sporulation, and the need for additional physiological tests make dermatophytes notoriously difficult to identify. The present study aimed to compare the ...

    journal_title：Journal de mycologie medicale

    pub_type： 杂志文章

    doi：10.1016/j.mycmed.2014.10.022

    authors： Ahmadi B,Mirhendi H,Shidfar MR,Nouripour-Sisakht S,Jalalizand N,Geramishoar M,Shokoohi GR

    更新日期：2015-03-01 00:00:00