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In vitro activity of carvacrol, cinnamaldehyde and thymol combined with antifungals against Malassezia pachydermatis.

Abstract：

:Malassezia pachydermatis is an important opportunistic agent of dermatitis and otitis in dogs. M. pachydermatis is generally treated with topical therapies using combinations of antifungal, antimicrobial and anti-inflammatory agents. We investigated the in vitro activities of carvacrol (CRV), cinnamaldehyde (CIN) and thymol (THY) alone and in combination with antifungal agents (fluconazole, itraconazole, ketoconazole, clotrimazole, miconazole, terbinafine and nystatin) against M. pachydermatis. The assays were performed according to the Clinical and Laboratory Standards Institute (CLSI), using Sabouraud dextrose broth and checkerboard microdilution. The mean fractional inhibitory concentration index (FICI) showed primary synergies for the combinations carvacrol+nystatin, thymol+nystatin, and carvacrol+miconazole (80%). In conclusion, the results obtained indicate that the phytochemicals tested showed relevant in vitro anti-M. pachydermatis activity. Future in vivo experiments are needed to elucidate the safety and therapeutic potential of these combinations.

journal_name

J Mycol Med

journal_title

Journal de mycologie medicale

authors

Schlemmer KB,Jesus FPK,Tondolo JSM,Weiblen C,Azevedo MI,Machado VS,Botton SA,Alves SH,Santurio JM

doi

10.1016/j.mycmed.2019.08.003

subject

Has Abstract

pub_date

2019-12-01 00:00:00

pages

375-377

issue

4

eissn

1156-5233

issn

1773-0449

pii

S1156-5233(19)30011-3

journal_volume

29

pub_type

杂志文章

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