当前位置： SCI文献检索 > BONE期刊下所有文献 > BMP9 and COX-2 form an important regulatory loop in BMP9-induced osteogenic differentiation of mesenchymal stem cells.

BMP9 and COX-2 form an important regulatory loop in BMP9-induced osteogenic differentiation of mesenchymal stem cells.

Abstract：

:Mesenchymal stem cells (MSCs) can self-renew and differentiate into osteogenic, chondrogenic, adipogenic and myogenic lineages. It's reported that bone morphogenetic protein 9 (BMP9) is one of the most potent osteogenic BMPs to initiate the commitment of MSCs to osteoblast lineage. Cyclooxygenase-2 (COX-2) is critical for bone fracture healing and osteogenic differentiation in MSCs. However, the relationship between COX-2 and BMP9 in osteogenesis remains unknown. Herein, we investigate the role of COX-2 in BMP9-induced osteogenesis in MSCs. We demonstrate that COX-2 is up-regulated as a target of BMP9 in MSCs. Both COX-2 inhibitor (NS-398) and COX-2 knockdown siRNAs can effectively decrease alkaline phosphatase (ALP) activities induced by BMP9 in MSCs. NS-398 also down-regulates BMP9-induced expression of osteopontin and osteocalcin, so does the matrix mineralization. The in vivo studies indicate that knockdown of COX-2 attenuates BMP9-induced ectopic bone formation. In perinatal limb culture assay, NS-398 is shown to reduce the hypertropic chondrocyte zone and ossification induced by BMP9. Mechanistically, knockdown of COX-2 significantly inhibits the BMP9 up-regulated expression of Runx2 and Dlx-5 in MSCs, which can be rescued by exogenous expression of COX-2. Furthermore, knockdown of COX-2 apparently reduces BMP9 induced BMPR-Smad reporter activity, the phosphorylation of Smad1/5/8, and the expression of Smad6 and Smad7 in MSCs. NS-398 blocks the expression of BMP9 mediated by BMP9 recombinant adenovirus. Taken together, our findings suggest that COX-2 plays an important role in BMP9 induced osteogenic differentiation in MSCs; BMP9 and COX-2 may form an important regulatory loop to orchestrate the osteogenic differentiation in MSCs.

journal_name

Bone

journal_title

Bone

authors

Wang JH,Liu YZ,Yin LJ,Chen L,Huang J,Liu Y,Zhang RX,Zhou LY,Yang QJ,Luo JY,Zuo GW,Deng ZL,He BC

doi

10.1016/j.bone.2013.08.015

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

311-21

issue

1

eissn

8756-3282

issn

1873-2763

pii

S8756-3282(13)00330-X

journal_volume

57

pub_type

杂志文章

相关文献

BONE文献大全
  • Histomorphometric changes in the trabecular structure of a selected stress region in the femur in patients with osteoarthritis and fracture of the femoral neck.

    abstract：:Histomorphometric changes in the trabecular structure of the principal compressive stress region in the femur in patients with osteoarthritis and fracture of the femoral neck are described. Femoral heads were obtained from patients who had surgery for the treatment of femoral neck fracture or total hip replacement for...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(85)90044-4

    authors： Fazzalari NL,Darracott J,Vernon-Roberts B

    更新日期：1985-01-01 00:00:00

  • Pathological fracture risk assessment in patients with femoral metastases using CT-based finite element methods. A retrospective clinical study.

    abstract：:Physician recommendation for prophylactic surgical fixation of a femur with metastatic bone disease (MBD) is usually based on Mirels' criteria and clinical experience, both of which suffer from poor specificity. This may result in a significant number of these health compromised patients undergoing unnecessary surgery...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2018.02.011

    authors： Sternheim A,Giladi O,Gortzak Y,Drexler M,Salai M,Trabelsi N,Milgrom C,Yosibash Z

    更新日期：2018-05-01 00:00:00

  • Methodology of MEDOS multicentre study of hip fracture incidence: validity and relevance considerations.

    abstract：:The Mediterranean osteoporosis (MEDOS) study was carried out in 14 centres from six countries in Southern Europe to determine the incidence rates and risk factors associated with hip fracture over the age of 50 years. This paper discusses both the validity and relevance of the data, that is, whether the number of coll...

    journal_title：Bone

    pub_type： 杂志文章,多中心研究

    doi：10.1016/8756-3282(93)90349-f

    authors： Elffors L,Gullberg B,Allander E,Johnell O,Kanis JA

    更新日期：1993-01-01 00:00:00

  • Pyruvate dehydrogenase kinase 4 induces bone loss at unloading by promoting osteoclastogenesis.

    abstract：:Disuse osteoporosis, which occurs commonly in prolonged bed rest and immobilization, is becoming a major problem in modern societies; however, the molecular mechanisms underlying unloading-driven bone loss have not been fully elucidated. The osteocyte network is considered to be an ideal mechanosensor and mechanotrans...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2011.07.012

    authors： Wang Y,Liu W,Masuyama R,Fukuyama R,Ito M,Zhang Q,Komori H,Murakami T,Moriishi T,Miyazaki T,Kitazawa R,Yoshida CA,Kawai Y,Izumi S,Komori T

    更新日期：2012-01-01 00:00:00

  • Early detection of burn induced heterotopic ossification using transcutaneous Raman spectroscopy.

    abstract：INTRODUCTION:Heterotopic ossification (HO), or the abnormal formation of bone in soft tissue, occurs in over 60% of major burn injuries and blast traumas. A significant need exists to improve the current diagnostic modalities for HO which are inadequate to diagnose and intervene on HO at early time-points. Raman spectr...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2013.01.002

    authors： Peterson JR,Okagbare PI,De La Rosa S,Cilwa KE,Perosky JE,Eboda ON,Donneys A,Su GL,Buchman SR,Cederna PS,Wang SC,Kozloff KM,Morris MD,Levi B

    更新日期：2013-05-01 00:00:00

  • Contributions of bone density and geometry to the strength of the human second metatarsal.

    abstract：:We investigated, at the whole bone level, the contribution of bone density and geometry to the fracture load of the second metatarsal, a bone that is prone to stress fracture. Dual-energy X-ray absorptiometry (DXA) was used to determine the areal bone mineral density (BMD), projected area of bone, and bone mineral con...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(00)00370-7

    authors： Muehleman C,Lidtke R,Berzins A,Becker JH,Shott S,Sumner DR

    更新日期：2000-11-01 00:00:00

  • Paget's disease and osteoclast biology.

    abstract：:Paget's disease is the most exaggerated example of bone remodeling in which abnormal osteoclastic bone resorption remains coupled to new bone formation. There are abnormalities in the stages of osteoclast development, and studies in Paget's disease have suggested a major role for IL-6 in human osteoclast activity. The...

    journal_title：Bone

    pub_type： 杂志文章,评审

    doi：10.1016/8756-3282(96)00211-6

    authors： Roodman GD

    更新日期：1996-09-01 00:00:00

  • Transosseous application of low-intensity ultrasound for the enhancement and monitoring of fracture healing process in a sheep osteotomy model.

    abstract：:The purpose of this study is twofold: (a) to investigate the application of transosseous low-intensity pulsed ultrasound (LiUS) on the enhancement of fracture healing and (b) to demonstrate the ability of transosseous ultrasound propagation to monitor the healing process. A midshaft tibial osteotomy model was used on ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2005.10.012

    authors： Malizos KN,Papachristos AA,Protopappas VC,Fotiadis DI

    更新日期：2006-04-01 00:00:00

  • Measurement and estimation of osteocyte mechanical strain.

    abstract：:Osteocytes are the most abundant cell type in bone and are responsible for sensing mechanical strain and signaling bone (re)modeling, making them the primary mechanosensors within bone. Under aging and osteoporotic conditions, bone is known to be less responsive to loading (exercise), but it is unclear why. Perhaps, t...

    journal_title：Bone

    pub_type： 杂志文章,评审

    doi：10.1016/j.bone.2013.01.037

    authors： Stern AR,Nicolella DP

    更新日期：2013-06-01 00:00:00

  • Estrogen deficiency increases osteoclastogenesis up-regulating T cells activity: a key mechanism in osteoporosis.

    abstract：:Compelling evidences suggest that increased production of osteoclastogenic cytokines by activated T cells plays a relevant role in the bone loss induced by estrogen deficiency in the mouse. However, little information is available on the role of T cells in post-menopausal bone loss in humans. To investigate this issue...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2008.02.017

    authors： D'Amelio P,Grimaldi A,Di Bella S,Brianza SZM,Cristofaro MA,Tamone C,Giribaldi G,Ulliers D,Pescarmona GP,Isaia G

    更新日期：2008-07-01 00:00:00

  • Positive effect of low dose vitamin D supplementation on growth of fetal bones: A randomized prospective study.

    abstract：:The effect of vitamin D supplementation on growth of fetal bones during pregnancy is unclear. The aim of this study was to assess the effect of low dose vitamin D supplementation during pregnancy on bony anthropometric aspects of the fetus. In this prospective randomized trial, 140 patients were divided into two equal...

    journal_title：Bone

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.bone.2019.02.022

    authors： Vafaei H,Asadi N,Kasraeian M,Shahraki HR,Bazrafshan K,Namazi N

    更新日期：2019-05-01 00:00:00

  • Increased prevalence of fractures in inadequately transfused and chelated Indian children and young adults with beta thalassemia major.

    abstract：INTRODUCTION:In patients with beta thalassemia major, inadequate transfusion and chelation may compromise bone health and increase risk of fractures. The objective of this study was to describe the prevalence of fractures in Indian inadequately transfused and chelated children, adolescents and young adults with beta th...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115649

    authors： Ekbote V,Padidela R,Khadilkar V,Ramanan V,Maheshwari A,Mughal Z,Kariki EP,Crabtree N,Khadilkar A

    更新日期：2021-02-01 00:00:00

  • Effects of teriparatide on cortical histomorphometric variables in postmenopausal women with or without prior alendronate treatment.

    abstract：:Cortical bone, the dominant component of the human skeleton by volume, plays a key role in protecting bones from fracture. We analyzed the cortical bone effects of teriparatide treatment in postmenopausal women with osteoporosis who had previously received long-term alendronate (ALN) therapy or were treatment naïve (T...

    journal_title：Bone

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.bone.2013.11.011

    authors： Ma YL,Zeng QQ,Chiang AY,Burr D,Li J,Dobnig H,Fahrleitner-Pammer A,Michalská D,Marin F,Pavo I,Stepan JJ

    更新日期：2014-02-01 00:00:00

  • High levels of serum IL-18 promote cartilage loss through suppression of aggrecan synthesis.

    abstract：:Osteoarthritis (OA) is closely related to the function of several inflammatory cytokines. It has been reported that older age is associated with higher serum levels of the inflammatory cytokine IL-18. In the present study, we investigated the long-term role of serum IL-18 in cartilage loss in vivo using a new strain o...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2008.01.031

    authors： Inoue H,Hiraoka K,Hoshino T,Okamoto M,Iwanaga T,Zenmyo M,Shoda T,Aizawa H,Nagata K

    更新日期：2008-06-01 00:00:00

  • Ex vivo regional gene therapy with human adipose-derived stem cells for bone repair.

    abstract：BACKGROUND:The treatment of complex bone loss scenarios remains challenging. This study evaluates the efficacy of ex vivo regional gene therapy using transduced human adipose-derived stem cells (ASCs) overexpressing bone morphogenetic protein-2 (BMP-2) to treat critical-sized bone defects. METHODS:Critical-sized femor...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115524

    authors： Vakhshori V,Bougioukli S,Sugiyama O,Kang HP,Tang AH,Park SH,Lieberman JR

    更新日期：2020-09-01 00:00:00

  • Alendronate has a residual effect on bone mass in postmenopausal Danish women up to 7 years after treatment withdrawal.

    abstract：:Alendronate has been shown to reduce bone turnover and increase bone mass. However, little is known about the duration of the effect on bone after treatment withdrawal. The aim of this study was to investigate the long-term effects on bone mineral density (BMD) and bone turnover of various alendronate regimens after t...

    journal_title：Bone

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s8756-3282(03)00112-1

    authors： Bagger YZ,Tankó LB,Alexandersen P,Ravn P,Christiansen C

    更新日期：2003-09-01 00:00:00

  • Estrogen-specific action on bone geometry and volumetric bone density: longitudinal observations in an adult with complete androgen insensitivity.

    abstract：INTRODUCTION:Sex steroids have distinct effects on bone growth and maintenance in men and women, mediated through their respective steroid receptors. Though most evidence is derived from animal studies, several concepts have been confirmed in humans by detection of specific mutations. In this report we describe changes...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2009.04.198

    authors： Taes Y,Lapauw B,Vandewalle S,Zmierczak H,Goemaere S,Vanderschueren D,Kaufman JM,T'Sjoen G

    更新日期：2009-08-01 00:00:00

  • Treatment with eldecalcitol positively affects mineralization, microdamage, and collagen crosslinks in primate bone.

    abstract：:Eldecalcitol (ELD), an active form of vitamin D analog approved for the treatment of osteoporosis in Japan, increases lumbar spine bone mineral density (BMD), suppresses bone turnover markers, and reduces fracture risk in patients with osteoporosis. We have previously reported that treatment with ELD for 6 months impr...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2014.11.025

    authors： Saito M,Grynpas MD,Burr DB,Allen MR,Smith SY,Doyle N,Amizuka N,Hasegawa T,Kida Y,Marumo K,Saito H

    更新日期：2015-04-01 00:00:00

  • Perinatal hypophosphatasia caused by uniparental isodisomy.

    abstract：:Hypophosphatasia (HPP) is an inherited disorder characterized by defective bone mineralization caused by mutations in the alkaline phosphatase gene (ALPL). Clinically, the disease spans a great continuum of disease severity and six forms can be distinguished according to the age of onset. The most severe is the autoso...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2013.12.009

    authors： Watanabe A,Satoh S,Fujita A,Naing BT,Orimo H,Shimada T

    更新日期：2014-03-01 00:00:00

  • SIBLING family genes and bone mineral density: association and allele-specific expression in humans.

    abstract：:Osteoporosis is a common complex disorder with reduced bone mineral density (BMD) and increased susceptibility to fracture. Peak BMD is one of the primary determinants of osteoporotic fracture risk, and is under substantial genetic control. Extracellular matrix, a major component of the bone, influences BMD by regulat...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2014.04.013

    authors： Alam I,Padgett LR,Ichikawa S,Alkhouli M,Koller DL,Lai D,Peacock M,Xuei X,Foroud T,Edenberg HJ,Econs MJ

    更新日期：2014-07-01 00:00:00

  • CSF-1 blockade impairs breast cancer osteoclastogenic potential in co-culture systems.

    abstract：:Metastatic bone disease has a major impact on the morbidity and mortality of breast cancer patients, and studies on bone metastasis biology have led to the development of the most widely used drugs for bone metastases treatment: zoledronate (Zol) and denosumab (Den). The aim of the present study was to assess the effe...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2014.06.017

    authors： Liverani C,Mercatali L,Spadazzi C,La Manna F,De Vita A,Riva N,Calpona S,Ricci M,Bongiovanni A,Gunelli E,Zanoni M,Fabbri F,Zoli W,Amadori D,Ibrahim T

    更新日期：2014-09-01 00:00:00

  • Hematopoietic stem cell transplantation-induced bone remodeling in autosomal recessive osteopetrosis: Interaction between skeleton and hematopoietic and sensory nervous systems.

    abstract：OBJECTIVE:Autosomal recessive osteopetrosis (ARO) is a rare congenital disorder of defective bone resorption. The inability of osteoclasts to resorb bone compromises the development of bone marrow cavity, and ultimately, leads to defective hematopoiesis and death within the first decade. The only curative treatment cur...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.115144

    authors： Maximova N,Zennaro F,Gregori M,Boz G,Zanon D,Mbalaviele G

    更新日期：2020-01-01 00:00:00

  • The generation of highly enriched osteoclast-lineage cell populations.

    abstract：:Osteoclasts form when hematopoietic cells are stimulated by macrophage colony-stimulating factor (M-CSF) and receptor activator of NF-kappaB ligand (RANKL) or tumor necrosis factor-alpha (TNFalpha). Osteoclast precursors derive from M-CSF-dependent proliferating hematopoietic cells but cannot yet be purified from mixe...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(01)00654-8

    authors： Quinn JM,Whitty GA,Byrne RJ,Gillespie MT,Hamilton JA

    更新日期：2002-01-01 00:00:00

  • Genetic and environmental determinants of bone mineral density in Mexican Americans: results from the San Antonio Family Osteoporosis Study.

    abstract：:Osteoporosis is a major cause of disability in the United States. Numerous factors contribute to the decline in bone mineral density (BMD) that characterizes this disease, and the importance of heredity is now widely appreciated. We evaluated the joint contributions of genes and environmental factors on variation in B...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(03)00246-1

    authors： Mitchell BD,Kammerer CM,Schneider JL,Perez R,Bauer RL

    更新日期：2003-11-01 00:00:00

  • Skeletal phenotype of mandibuloacral dysplasia associated with mutations in ZMPSTE24.

    abstract：:Mandibuloacral dysplasia (MAD) is a rare recessively inherited premature aging disease characterized by skeletal and metabolic anomalies. It is part of the spectrum of diseases called laminopathies and results from mutations in genes regulating the synthesis of the nuclear laminar protein, lamin A. Homozygous or compo...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2010.06.004

    authors： Cunningham VJ,D'Apice MR,Licata N,Novelli G,Cundy T

    更新日期：2010-09-01 00:00:00

  • Bringing new life to damaged bone: the importance of angiogenesis in bone repair and regeneration.

    abstract：:Bone has the unique capacity to heal without the formation of a fibrous scar, likely because several of the cellular and molecular processes governing bone healing recapitulate the events during skeletal development. A critical component in bone healing is the timely appearance of blood vessels in the fracture callus....

    journal_title：Bone

    pub_type： 杂志文章,评审

    doi：10.1016/j.bone.2014.09.017

    authors： Stegen S,van Gastel N,Carmeliet G

    更新日期：2015-01-01 00:00:00

  • Quantification of remodeling parameter sensitivity--assessed by a computer simulation model.

    abstract：:During normal aging and menopause, cancellous bone is lost at all skeletal sites due to remodeling-related factors: negative formation balance; temporarily increased remodeling space; and osteoclastic perforations. The relative importance of the various factors in inducing bone mass loss and perforations is still cont...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(96)00231-1

    authors： Thomsen JS,Mosekilde L,Mosekilde E

    更新日期：1996-11-01 00:00:00

  • Hypophosphatasia: validation and expansion of the clinical nosology for children from 25 years experience with 173 pediatric patients.

    abstract：:Hypophosphatasia (HPP) is caused by loss-of-function mutation(s) within the gene TNSALP that encodes the "tissue-nonspecific" isoenzyme of alkaline phosphatase (TNSALP). In HPP, inorganic pyrophosphate, an inhibitor of mineralization and substrate for TNSALP, accumulates extracellularly often leading to rickets or ost...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2015.02.022

    authors： Whyte MP,Zhang F,Wenkert D,McAlister WH,Mack KE,Benigno MC,Coburn SP,Wagy S,Griffin DM,Ericson KL,Mumm S

    更新日期：2015-06-01 00:00:00

  • Three-dimensional cultures of normal human osteoblasts: proliferation and differentiation potential in vitro and upon ectopic implantation in nude mice.

    abstract：:We report the establishment in vitro of three-dimensional (3D) cultures of human osteoblasts (hOB) derived from normal adults and supported uniquely by the extracellular matrix (ECM) they deposit. Osteoblasts were cultured in 3D cultures in vitro for up to 120 days. The 3D cultures, examined at 25, 31, and 48 days, ex...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(02)00691-9

    authors： Ferrera D,Poggi S,Biassoni C,Dickson GR,Astigiano S,Barbieri O,Favre A,Franzi AT,Strangio A,Federici A,Manduca P

    更新日期：2002-05-01 00:00:00

  • Prevention of glucocorticoid induced-apoptosis of osteoblasts and osteocytes by protecting against endoplasmic reticulum (ER) stress in vitro and in vivo in female mice.

    abstract：:Endoplasmic reticulum (ER) stress is associated with increased reactive oxygen species (ROS), results from accumulation of misfolded/unfolded proteins, and can trigger apoptosis. ER stress is alleviated by phosphorylation of eukaryotic translation initiation factor 2α (eIF2α), which inhibits protein translation allowi...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2014.12.012

    authors： Sato AY,Tu X,McAndrews KA,Plotkin LI,Bellido T

    更新日期：2015-04-01 00:00:00