当前位置： SCI文献检索 > BONE期刊下所有文献 > The generation of highly enriched osteoclast-lineage cell populations.

The generation of highly enriched osteoclast-lineage cell populations.

Abstract：

:Osteoclasts form when hematopoietic cells are stimulated by macrophage colony-stimulating factor (M-CSF) and receptor activator of NF-kappaB ligand (RANKL) or tumor necrosis factor-alpha (TNFalpha). Osteoclast precursors derive from M-CSF-dependent proliferating hematopoietic cells but cannot yet be purified from mixed populations. M-CSF stimulation of bone marrow cells results in large numbers of nonadherent, proliferating macrophage precursors. These rapidly form adherent bone marrow macrophages (BMM). BMM and their precursors can be isolated free from mesenchymal and lymphocytic cells. BMM precursors derived from CBA-strain mouse bone marrow, when cocultured with ST2 cells (which express RANKL and M-CSF), formed numerous mononuclear osteoclasts, which resorbed bone and expressed tartrate-resistant acid phosphatase (TRAP) and calcitonin receptors (CTR). Addition of approximately 10 BMM precursors to ST2 cultures resulted in over 80% of these cocultures forming functional osteoclasts, suggesting that they are a highly enriched source of osteoclast progenitors. Supporting this, recombinant RANKL/M-CSF-stimulated BMM precursors formed populations in which all cells expressed TRAP. While only a small proportion of these cells (8.6%) expressed CTR, with transforming growth factor-beta (TGFbeta) present RANKL/M-CSF-stimulated BMM precursors formed almost pure (98.4%) CTR-positive osteoclasts after 7 days. This suggests that TGFbeta stimulated the maturation rate of these cells. Passaged or viably frozen BMM precursors gave rise to BMM that also all formed osteoclasts lineage cells after RANKL/M-CSF stimulation. These data suggest that BMM precursors derived from CBA mice are an expanded pool of osteoclast progenitors. These can be employed to generate osteoclast populations of high purity and in large numbers when stimulated by TGFbeta, which greatly augments the osteoclastogenic effects of RANKL.

journal_name

Bone

journal_title

Bone

authors

Quinn JM,Whitty GA,Byrne RJ,Gillespie MT,Hamilton JA

doi

10.1016/s8756-3282(01)00654-8

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

164-70

issue

1

eissn

8756-3282

issn

1873-2763

pii

S8756328201006548

journal_volume

30

pub_type

杂志文章

相关文献

BONE文献大全
  • Identification of differentially expressed cDNA transcripts from a rat odontoblast cell line.

    abstract：:Odontoblasts and osteoblasts are two among the myriads of cell types present in the craniofacial complex. Both have a common ectomesenchymal origin and secrete macromolecules that are necessary for the formation of dentin and alveolar bone via matrix-mediated mechanisms. The mineralized matrices of bone and dentin dif...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2005.05.013

    authors： Hao J,He G,Narayanan K,Zou B,Lin L,Muni T,Ramachandran A,George A

    更新日期：2005-10-01 00:00:00

  • Revealing the interplay of bone and cartilage in osteoarthritis through multimodal imaging of murine joints.

    abstract：:Osteoarthritis (OA) affects both cartilage and bone tissues, and the subsequent breakdown of the two tissues appears to be interrelated. The interest in the role of subchondral bone changes with OA is growing, and one suggestion is that a simple inverse correlation exists between the cartilage loss and increased bone ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2009.05.017

    authors： Stok KS,Pelled G,Zilberman Y,Kallai I,Goldhahn J,Gazit D,Müller R

    更新日期：2009-09-01 00:00:00

  • Modulation of bone resorption by glycosaminoglycans: effects of parathyroid hormone and interleukin-1.

    abstract：:Regulation of calcium release from mouse calvariae by glycosaminoglycans and known modulators of bone resorption has been examined. Anticoagulant and nonanticoagulant fragments of heparin were combined with parathyroid hormone (PTH) and interleukin-1, beta (IL1), and calcium release from radiolabeled bone was determin...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(88)90018-x

    authors： Cochran DL,Abernathy CK

    更新日期：1988-01-01 00:00:00

  • Interrelation between external oscillatory muscle coupling amplitude and in vivo intramedullary pressure related bone adaptation.

    abstract：:Interstitial bone fluid flow (IBFF) is suggested as a communication medium that bridges external physical signals and internal cellular activities in the bone, which thus regulates bone remodeling. Intramedullary pressure (ImP) is one main regulatory factor of IBFF and bone adaptation related mechanotransduction. Our ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2014.05.018

    authors： Hu M,Cheng J,Bethel N,Serra-Hsu F,Ferreri S,Lin L,Qin YX

    更新日期：2014-09-01 00:00:00

  • Comparison in localization between cystatin C and cathepsin K in osteoclasts and other cells in mouse tibia epiphysis by immunolight and immunoelectron microscopy.

    abstract：:We compared the distribution of a cysteine proteinase inhibitor, cystatin C, with that of cathepsin K in osteoclasts of the mouse tibia by immunolight and immunoelectron microscopy. Light microscopically, strong immunoreactivity for cystatin C was found extracellularly along the resorption lacuna and intracellularly i...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(01)00466-5

    authors： Yamaza T,Tsuji Y,Goto T,Kido MA,Nishijima K,Moroi R,Akamine A,Tanaka T

    更新日期：2001-07-01 00:00:00

  • Granulocyte-macrophage colony-stimulating factor-dependent CD11c-positive cells differentiate into active osteoclasts.

    abstract：:Levels of circulating cytokines are elevated in inflammatory diseases. Previously, it was shown that interleukin (IL-)17A, in synergism with 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] and tumor necrosis factor α (TNFα), induces the release of granulocyte-macrophage colony-stimulating factor (GM-CSF) by murine osteoblasts ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2017.01.036

    authors： Ruef N,Dolder S,Aeberli D,Seitz M,Balani D,Hofstetter W

    更新日期：2017-04-01 00:00:00

  • Postnatal β-catenin deletion from Dmp1-expressing osteocytes/osteoblasts reduces structural adaptation to loading, but not periosteal load-induced bone formation.

    abstract：:Mechanical signal transduction in bone tissue begins with load-induced activation of several cellular pathways in the osteocyte population. A key pathway that participates in mechanotransduction is Wnt/Lrp5 signaling. A putative downstream mediator of activated Lrp5 is the nucleocytoplasmic shuttling protein β-catenin...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2016.04.028

    authors： Kang KS,Hong JM,Robling AG

    更新日期：2016-07-01 00:00:00

  • Selective enrichment of microRNAs in extracellular matrix vesicles produced by growth plate chondrocytes.

    abstract：:Matrix vesicles (MVs) are membrane organelles found in the extracellular matrix of calcifying cells, which contain matrix processing enzymes and regulate the extracellular environment via action of these enzymes. It is unknown whether MVs are also exosomic mediators of cell-cell communication via transfer of RNA mater...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2016.03.018

    authors： Lin Z,Rodriguez NE,Zhao J,Ramey AN,Hyzy SL,Boyan BD,Schwartz Z

    更新日期：2016-07-01 00:00:00

  • Isolation of osteoclasts from Pagetic bone tissue: morphometry and cytochemistry on isolated cells.

    abstract：:Giant osteoclasts and other cells were isolated from Pagetic bone tissue using 0.5 mM ethylene diamine tetraacetic acid on bone samples from 8 patients with Paget's disease. The cell suspension contained osteoclasts and osteoblasts as well as some mononuclear cells such as monocytes. The number of nuclei in isolated o...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(88)90020-8

    authors： Basle MF,Mazaud P,Malkani K,Chretien MF,Moreau MF,Rebel A

    更新日期：1988-01-01 00:00:00

  • Early detection of burn induced heterotopic ossification using transcutaneous Raman spectroscopy.

    abstract：INTRODUCTION:Heterotopic ossification (HO), or the abnormal formation of bone in soft tissue, occurs in over 60% of major burn injuries and blast traumas. A significant need exists to improve the current diagnostic modalities for HO which are inadequate to diagnose and intervene on HO at early time-points. Raman spectr...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2013.01.002

    authors： Peterson JR,Okagbare PI,De La Rosa S,Cilwa KE,Perosky JE,Eboda ON,Donneys A,Su GL,Buchman SR,Cederna PS,Wang SC,Kozloff KM,Morris MD,Levi B

    更新日期：2013-05-01 00:00:00

  • A novel autologous bone graft substitute comprised of rhBMP6 blood coagulum as carrier tested in a randomized and controlled Phase I trial in patients with distal radial fractures.

    abstract：:Bone morphogenetic proteins (BMPs) are known to induce new bone formation in vivo but treating trabecular bone defects with a BMP based therapeutic remains controversial. Here, we evaluated the safety and efficacy of a novel Autologous Bone Graft Substitute (ABGS) comprised of recombinant human BMP6 (rhBMP6) dispersed...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115551

    authors： Durdevic D,Vlahovic T,Pehar S,Miklic D,Oppermann H,Bordukalo-Niksic T,Gavrankapetanovic I,Jamakosmanovic M,Milosevic M,Martinovic S,Sampath TK,Peric M,Grgurevic L,Vukicevic S

    更新日期：2020-11-01 00:00:00

  • Circulating sclerostin is elevated in short-term and reduced in long-term SCI.

    abstract：:Spinal cord injury (SCI) causes profound bone loss due to muscle paralysis resulting in the inability to walk. Sclerostin, a Wnt signaling pathway antagonist produced by osteocytes, is a potent inhibitor of bone formation. Short-term studies in rodent models have demonstrated increased sclerostin in response to mechan...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2012.04.019

    authors： Battaglino RA,Sudhakar S,Lazzari AA,Garshick E,Zafonte R,Morse LR

    更新日期：2012-09-01 00:00:00

  • Increased sensitivity of rheumatoid synoviocytes to Schnurri-3 expression in TNF-α and IL-17A induced osteoblastic differentiation.

    abstract：OBJECTIVE:To compare the effects of TNF-α and IL-17A on osteogenic differentiation of isolated fibroblast-like synoviocytes (FLS) from healthy donors, osteoarthritis (OA) and rheumatoid arthritis (RA) patients. METHODS:FLS were cultured in osteogenic medium, with and without TNF-α and/or IL-17A. Extracellular matrix m...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2016.04.008

    authors： Lavocat F,Osta B,Miossec P

    更新日期：2016-06-01 00:00:00

  • Effect of zoledronate acid treatment on osseointegration and fixation of implants in autologous iliac bone grafts in ovariectomized rabbits.

    abstract：:One main problem associated with alveolar bone augmentation in implant dentistry is resorption of grafted bone, which may be further compromised by systemic skeletal disorders such as osteoporosis. Zoledronate acid (ZOL) is the most potent bisphosphonate to treat osteoporosis and therefore it is hypothesized to be abl...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2011.10.011

    authors： Qi M,Hu J,Li J,Li J,Dong W,Feng X,Yu J

    更新日期：2012-01-01 00:00:00

  • A comparative analysis of articular bone in large cohort of patients with chronic inflammatory diseases of the joints, the gut and the skin.

    abstract：:Chronic inflammatory diseases are associated with bone loss. While the occurrence of systemic bone loss is well described in chronic inflammatory diseases, the impact of these conditions on articular bone has not been systematically investigated. Recent refinements in high-resolution CT assessment of the joints now al...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2018.07.017

    authors： Simon D,Kleyer A,Englbrecht M,Stemmler F,Simon C,Berlin A,Kocijan R,Haschka J,Hirschmann S,Atreya R,Neurath MF,Sticherling M,Rech J,Hueber AJ,Engelke K,Schett G

    更新日期：2018-11-01 00:00:00

  • Bone material strength index is associated with prior fracture in men with and without moderate chronic kidney disease.

    abstract：BACKGROUND:Patients with chronic kidney disease (CKD) are at high risk for fracture. The ability of bone mineral density (BMD) to predict fractures in CKD patients has been inconsistent. Other measures such as trabecular bone score (TBS) and impact microindentation (IMI) may be more useful in this group. This study aim...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115241

    authors： Holloway-Kew KL,Rufus-Membere P,Anderson KB,Betson A,Gaston J,Kotowicz MA,Diez-Perez A,Hyde NK,Pasco JA

    更新日期：2020-04-01 00:00:00

  • Nanometer-scale features on micrometer-scale surface texturing: a bone histological, gene expression, and nanomechanical study.

    abstract：:Micro- and nanoscale surface modifications have been the focus of multiple studies in the pursuit of accelerating bone apposition or osseointegration at the implant surface. Here, we evaluated histological and nanomechanical properties, and gene expression, for a microblasted surface presenting nanometer-scale texture...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2014.05.004

    authors： Coelho PG,Takayama T,Yoo D,Jimbo R,Karunagaran S,Tovar N,Janal MN,Yamano S

    更新日期：2014-08-01 00:00:00

  • Effects of recreational team handball on bone health, postural balance and body composition in inactive postmenopausal women - A randomised controlled trial.

    abstract：:This study reports the effects of a recreational team handball exercise programme (randomised controlled trial, RCT) on bone health, postural balance and body composition in inactive postmenopausal women without previous experience of the sport. Sixty-seven postmenopausal women (68.3 ± 6.2 years, stature 156.9 ± 5.8 c...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2021.115847

    authors： Pereira R,Krustrup P,Castagna C,Coelho E,Santos R,Helge EW,Jørgensen NR,Magalhães J,Póvoas S

    更新日期：2021-01-13 00:00:00

  • Bisphosphonates--history and experimental basis.

    abstract：:This paper reviews the mechanisms of action of the bisphosphonates, a new class of recently developed drugs. The geminal bisphosphonates are characterized by a P-C-P bond and are thus analogs of pyrophosphate. They bind strongly to hydroxyapatite crystals and in vitro inhibit both crystal formation and dissolution. In...

    journal_title：Bone

    pub_type： 历史文章,杂志文章,评审

    doi：

    authors： Fleisch H

    更新日期：1987-01-01 00:00:00

  • Tetracycline/flurbiprofen combination therapy modulates bone remodeling in ovariectomized rats: preliminary observations.

    abstract：:The loss of trabecular bone in the ovariectomized (OVX) rat provides a useful experimental model of postmenopausal osteoporosis. In this study, two bone-modulating compounds, an NSAID (flurbiprofen: FBP) and a chemically modified nonantimicrobial tetracycline (CMT), were tested either individually or in combination in...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(96)00280-3

    authors： Aoyagi M,Sasaki T,Ramamurthy NS,Golub LM

    更新日期：1996-12-01 00:00:00

  • Sclerostin antibody increases bone mass by stimulating bone formation and inhibiting bone resorption in a hindlimb-immobilization rat model.

    abstract：:Sclerostin monoclonal antibody (Scl-Ab) has been shown to increase bone mass and bone strength by stimulating bone formation in an ovariectomy-induced bone loss rat model. The purpose of this study was to determine the effects of Scl-Ab in a rat immobilization/disuse model in which there was both a decrease in bone fo...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2010.09.009

    authors： Tian X,Jee WS,Li X,Paszty C,Ke HZ

    更新日期：2011-02-01 00:00:00

  • Longitudinal effects of fat and lean mass on bone accrual in infants.

    abstract：:There are conflicting reports on the influence of lean and fat mass on bone accrual during childhood. No infant's studies have been reported that describe the influence of changes in body composition with changes in bone accrual during the first year of life. The objective of this research was to test the hypothesis t...

    journal_title：Bone

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.bone.2011.11.011

    authors： Sudhagoni RG,Wey HE,Djira GD,Specker BL

    更新日期：2012-03-01 00:00:00

  • Ultrasonic characterization of human cancellous bone using transmission and backscatter measurements: relationships to density and microstructure.

    abstract：:The present study was designed to evaluate the relationships between ultrasonic backscatter, density, and microarchitecture of cancellous bone. The slopes of the frequency-dependent attenuation coefficient (nBUA), ultrasound bone velocity (UBV), the frequency-averaged backscatter coefficient (BUB) were measured in 25 ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(01)00650-0

    authors： Chaffaî S,Peyrin F,Nuzzo S,Porcher R,Berger G,Laugier P

    更新日期：2002-01-01 00:00:00

  • Dexamethasone inhibits and thyroid hormone promotes differentiation of mouse chondrogenic ATDC5 cells.

    abstract：:The effects of glucocorticoid (GC) excess, thyrotoxicosis, and hypothyroidism on linear growth indicate that growth plate chondrocytes are exquisitely sensitive to GC and thyroid hormone (T(3)). Murine ATDC5 cells undergo chondrogenesis in vitro and were used to evaluate the effects of dexamethasone (Dex) and T(3) on ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(02)00855-4

    authors： Siebler T,Robson H,Shalet SM,Williams GR

    更新日期：2002-10-01 00:00:00

  • Expression of bone microsomal casein kinase II, bone sialoprotein, and osteopontin during the repair of calvarial defects.

    abstract：:The temporal expression of bone microsomal casein kinase II, osteopontin, bone sialoprotein, alkaline phosphatase, and the accumulation of a solid calcium-inorganic orthophosphate mineral phase, have been charted from day 2 to day 21 during the repair of calvarial defects in rats induced by the implantation of decalci...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(98)00057-x

    authors： Wang J,Glimcher MJ,Mah J,Zhou HY,Salih E

    更新日期：1998-06-01 00:00:00

  • Differential turnover of cortical and trabecular bone in transgenic mice overexpressing cathepsin K.

    abstract：:Cathepsin K is a major osteoclastic protease. We have recently shown that overexpression of mouse cathepsin K gene in transgenic UTU17 mouse model results in high turnover osteopenia of metaphyseal trabecular bone at the age of 7 months. The present report extends these studies to a systematic analysis of cortical bon...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2005.02.006

    authors： Morko J,Kiviranta R,Hurme S,Rantakokko J,Vuorio E

    更新日期：2005-05-01 00:00:00

  • Effects of combined treatment with eldecalcitol and alendronate on bone mass, mechanical properties, and bone histomorphometry in ovariectomized rats: a comparison with alfacalcidol and alendronate.

    abstract：:Eldecalcitol (ELD), a 2β-hydroxypropyloxy derivative of 1α,25 (OH) 2D3, inhibits bone resorption more potently than alfacalcidol (ALF) while maintaining osteoblastic function in an ovariectomized (OVX) osteoporosis rat model. Alendronate (ALN), which is the most common bisphosphonate used for the treatment of osteopor...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2012.09.031

    authors： Sugimoto M,Futaki N,Harada M,Kaku S

    更新日期：2013-01-01 00:00:00

  • Interaction between human umbilical vein endothelial cells and human osteoprogenitors triggers pleiotropic effect that may support osteoblastic function.

    abstract：:Osteogenesis occurs in striking interaction with angiogenesis. There is growing evidence that endothelial cells are involved in the modulation of osteoblast differentiation. We hypothesized that primary human umbilical vein endothelial cells (HUVEC) should be able to modulate primary human osteoprogenitors (HOP) funct...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2008.01.025

    authors： Guillotin B,Bareille R,Bourget C,Bordenave L,Amédée J

    更新日期：2008-06-01 00:00:00

  • Age-related changes in vertebral trabecular bone architecture--assessed by a new method.

    abstract：:Cylindrical trabecular bone specimens (d = 7 mm) were drilled in a vertical direction from the central part of the third vertebral body (L3) from 23 normal individuals aged 15-87 years (10 males and 13 females). The bone samples were embedded in methylmetacrylate and sawn in 400 microns thick sections with an arbitrar...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(88)90038-5

    authors： Mosekilde L

    更新日期：1988-01-01 00:00:00

  • The collection of NFATc1-dependent transcripts in the osteoclast includes numerous genes non-essential to physiologic bone resorption.

    abstract：:Osteoclasts are specialized secretory cells of the myeloid lineage important for normal skeletal homeostasis as well as pathologic conditions of bone including osteoporosis, inflammatory arthritis and cancer metastasis. Differentiation of these multinucleated giant cells from precursors is controlled by the cytokine R...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2012.08.113

    authors： Charles JF,Coury F,Sulyanto R,Sitara D,Wu J,Brady N,Tsang K,Sigrist K,Tollefsen DM,He L,Storm D,Aliprantis AO

    更新日期：2012-11-01 00:00:00