Abstract：
:Ixodes affinis Neumann (1899) and Ixodes scapularis Say (1821) are tick vectors of the etiologic agent of Lyme disease, Borrelia burgdorferi sensu stricto. Ixodes affinis and I. scapularis are morphologically very similar, and as they are sympatric in the mid- and south-Atlantic U.S. coastal states, their accurate identification is crucial to studies of disease and vector ecology in this area. This work describes a rapid, single-tube SYBR(®) Green-based real-time PCR assay for differentiation of I. affinis and I. scapularis at all life stages. The assay employs 2 pairs of species-specific primers directed against the internal transcribed spacer 2 (ITS2) region of the nuclear rRNA operon. Amplification products for these primer pairs differ in size and may be differentiated with a melt curve analysis. This tool is intended as a supplement to morphological methods for accurate identification of these ticks.
journal_name
Ticks Tick Borne Disjournal_title
Ticks and tick-borne diseasesauthors
Wright CL,Hynes WL,White BT,Marshall MN,Gaff HD,Gauthier DTdoi
10.1016/j.ttbdis.2013.08.003subject
Has Abstractpub_date
2014-02-01 00:00:00pages
48-52issue
1eissn
1877-959Xissn
1877-9603pii
S1877-959X(13)00087-3journal_volume
5pub_type
杂志文章abstract：:The raccoon (Procyon lotor) and the raccoon dog (Nyctereutes procyonoides) belong to a group of the invasive species. The introduced species as potential reservoirs for vector-borne pathogens have been the subject of recent research, though there are still no data with reference to the European population of the racco...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.03.018
更新日期：2018-05-01 00:00:00
abstract：:The current work evaluated road-killed Pampas foxes (Lycalopex gymnocercus) and their ticks for the presence of vector-borne agents in the ecoregion Esteros del Iberá in northeastern Argentina. Spleen, lung and blood samples and Amblyomma tigrinum adult ticks collected from the foxes were tested by polymerase chain re...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.101318
更新日期：2020-03-01 00:00:00
abstract：:Theileriosis is a disease of domesticated animals in tropical and subtropical countries and causes significant reductions in livestock productivity. The arid region of Punjab in Pakistan is notorious for the presence of the vector tick (Acari: Ixodidae) and tick-borne diseases, such as theileriosis and babesiosis. The...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.03.028
更新日期：2018-05-01 00:00:00
abstract：:Immature Ixodes scapularis infestation and Borrelia burgdorferi infection of wild small mammals were studied from June to October in 2007 and from May to October in 2008 at 71 study sites in a zone where I. scapularis populations and environmental Lyme disease risk are emerging in southwestern Quebec. Seasonal host-se...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2011.03.005
更新日期：2011-12-01 00:00:00
abstract：:Tick-borne diseases have a complex epidemiology that depends on different ecological communities, associating several species of vertebrate hosts, vectors and pathogens. While most studies in Europe are focused on Ixodes ricinus, other Ixodes species may also be involved in the transmission or maintenance of pathogens...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2020.101625
更新日期：2021-03-01 00:00:00
abstract：:Rodents are hosts of numerous pathogenic agents of public health importance globally. Their ability to harbor these pathogens without showing overt clinical signs of disease has epidemiologic consequences. In some rural settings in Nigeria, humans and rodents do not only share feeds and abode, but the latter may end u...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.10.004
更新日期：2018-01-01 00:00:00
abstract：:For more than 50 years DEET (N,N-Diethyl-m-toluamide) has been considered the gold standard of repellents. It is applied to the skin or clothing to deter mosquitoes and other blood-sucking invertebrate pests from approaching and/or settling, and ultimately it provides temporary protection from bites. Despite rampant g...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.12.006
更新日期：2019-04-01 00:00:00
abstract：:Rickettsia rickettsii is the agent of Brazilian spotted fever (BSF), the most lethal tick-borne disease in the western hemisphere. Amblyomma sculptum is the main vector of R. rickettsii in southeastern Brazil. Capybaras act as primary hosts for this tick species, and as amplifying hosts for R. rickettsii, creating new...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2020.101463
更新日期：2020-09-01 00:00:00
abstract：:Crimean-Congo hemorrhagic fever virus (CCHFV) was detected in Spain in 2010. The presence of CCHFV in Hyalomma marginatum ticks from migratory birds passing through Morocco during the spring migration strengthened the hypothesis of the arrival of infected ticks transported by birds to the Iberian Peninsula. Furthermor...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2016.05.004
更新日期：2016-07-01 00:00:00
abstract：:Suspicion of Brazilian spotted fever (BSF) should occur in endemic regions upon surveillance of the acute febrile icteric hemorrhagic syndrome (AFIHS). However, limitations associated with currently available laboratory tests pose a challenge to early diagnosis, especially in fatal cases. Two real-time PCR (qPCR) prot...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2012.10.027
更新日期：2012-12-01 00:00:00
abstract：:A total of 7364 ticks belonging to 13 species was collected from 64 game animals (belonging to 11 species) and from 64 livestock animals (cattle and sheep) living in close vicinity at 6 localities in 3 South African Provinces (Free State, Mpumalanga, and Limpopo). The geographic distribution of all tick species was co...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2013.10.003
更新日期：2014-03-01 00:00:00
abstract：:The current study compared the virulence of conidia and blastospores of Metarhizium robertsii (IP 146), M. anisopliae sensu lato (s.l.) (IP 363) and Beauveria bassiana s.l. (IP 361 and CG 307) against unfed larvae and engorged females of Rhipicephalus microplus (Acari: Ixodidae). In addition, the development of fungal...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.06.001
更新日期：2018-07-01 00:00:00
abstract：:Equine piroplasmosis (EP) is an important tick-borne disease of equids, caused by Theileria equi and Babesia caballi. It is endemic in most parts of the world, including Israel, and has clinical and economic consequences. This study was set to evaluate the presence of EP parasites in domestic donkeys and in wild equid...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.101286
更新日期：2020-01-01 00:00:00
abstract：:The current need of identification of a new acaricidal agent which is acceptable to public as environmentally safe is a daring task. Use of herbal acaricides is such an alternative. Most of the herbal extracts or fractions are dissolved in polar or non-polar solvents or detergents before tested for acaricidal activity...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2011.04.001
更新日期：2011-09-01 00:00:00
abstract：:Natural enemies of ticks include the parasitoid wasp Ixodiphagus hookeri (Hymenoptera: Encyrtidae). The aim of this preliminary study was to investigate the occurrence of I. hookeri DNA in a community of ticks (Ixodes ricinus, Dermacentor marginatus, Hyalomma marginatum, Haemaphysalis inermis and Rhipicephalus turanic...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2015.01.001
更新日期：2015-04-01 00:00:00
abstract：:Ticks were collected from wild birds at 3 locations in Paraguay during the South American winter in August and September 2012. In total, 480 birds belonging to 106 species were examined. Overall, 31 (6.5%) birds representing 21 species were found parasitized by ticks which were identified as Amblyomma calcaratum Neuma...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2013.08.004
更新日期：2014-03-01 00:00:00
abstract：:The hard tick Ixodes ricinus is the principal vector of Lyme borreliosis (LB) group spirochaetes in Europe, but it also transmits a large number of other microbial pathogens that are of importance to animal and human health. Here, we characterise geographically distinct populations of this important ectoparasite based...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2013.10.001
更新日期：2014-03-01 00:00:00
abstract：:Tick-borne encephalitis virus (TBEV) is a zoonotic agent causing severe encephalitis in humans. A wide range of animal species could be infected with TBEV in endemic areas. A serological survey of wild animals is effective in identifying TBEV-endemic areas. Safe, simple, and reliable TBEV serodiagnostic tools are need...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2016.03.002
更新日期：2016-07-01 00:00:00
abstract：:The entomopathogenic fungus Metarhizium anisopliae is used as a biological pest control agent against various arthropod species, including ticks. However, the efficacy depends on tick species, tick stage and fungus strain. We studied the effect of M. anisopliae on engorged larvae and nymphs of Ixodes ricinus, the most...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2016.03.010
更新日期：2016-07-01 00:00:00
abstract：:Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne pathogen in China, Japan, and the Republic of Korea (ROK). The aim of this study was to investigate the prevalence of SFTSV antigens and anti-SFTSV antibodies in black goats (Capra hircus coreanae) throughout the ROK. Sera were collect...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.04.018
更新日期：2018-07-01 00:00:00
abstract：:Anaplasma phagocytophilum is an emerging zoonotic tick-borne pathogen affecting a wide range of mammals. Rodents are suspected to be natural reservoirs for this bacterium, but their role in the epidemiologic cycles affecting domestic animals and wild ungulates has not been demonstrated. This study aimed to improve our...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2016.05.005
更新日期：2016-07-01 00:00:00
abstract：:Crimean-Congo hemorrhagic fever (CCHF) is an acute tick-borne viral zoonotic disease which is endemic in Turkey. Bradycardia has been reported among pediatric and adult patients with CCHF. But, it remains unclear, whether bradycardia is associated with ribavirin treatment or the severity of CCHF. In this study 26 hosp...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2014.12.003
更新日期：2015-03-01 00:00:00
abstract：:Tick-borne diseases (TBD) occur in many temperate countries and are economically important in most tropical and subtropical areas, affecting dairy and beef cattle, as well as small ruminants. Four major tick-borne diseases have been detected in eastern and southern Africa: East Coast fever (ECF) caused by Theileria pa...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.101283
更新日期：2020-01-01 00:00:00
abstract：:Rickettsia amblyommatis is widely distributed in the Americas, and has been reported to infect different species of ticks within its distribution. In Panama, R. amblyommatis is the most common Rickettsia and its presence was molecularly detected in nine species of ticks and one flea species. This work described the is...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2020.101597
更新日期：2021-01-01 00:00:00
abstract：:Livestock and their ectoparasites are involved in the epidemiology of several zoonotic diseases. Studies regarding the molecular detection of infectious agents in ticks from Northwestern Algeria are scarce. Thus, the presence of spotted fever group Rickettsia spp., Anaplasmataceae microorganisms and Coxiella burnetii ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.04.018
更新日期：2019-06-01 00:00:00
abstract：:Lyme borreliosis (LB) is the most frequent vector-borne disease in Germany. For more than 10 years, data from mandatory notifications have been available from 6 federal states in the eastern part of Germany. A common case definition was applied. Clinical manifestations of erythema migrans, neuroborreliosis (radiculone...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2013.10.010
更新日期：2014-04-01 00:00:00
abstract：:We sought to determine the behavioral risk of exposure to tick-borne diseases across a human land-use gradient in a region endemic for diseases transmitted by the lone star tick. We measured the knowledge, attitudes, and preventive behaviors of visitors to 14 suburban, exurban, and rural recreational parks. A structur...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2013.01.001
更新日期：2013-06-01 00:00:00
abstract：:In this study, we examine the current pattern of tick diversity and host use in French Guiana, South America, from 97 sampling localities encompassing peri-urban, rural and natural habitats. We collected 3395 ticks, including 1485 specimens from 45 vertebrate species (humans, domestic and wild animals) and 1910 questi...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.09.003
更新日期：2019-01-01 00:00:00
abstract：:Lyme disease is the most commonly reported vector-borne illness in the United States. Since the institution of Nationally Notifiable surveillance efforts for Lyme disease in the United States in 1991, there has been a consistent increase in the number of reported cases. Thus, the need for targeted prevention strategie...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2015.12.003
更新日期：2016-03-01 00:00:00
abstract：:This is the first reported case of Babesia sp. in Antarctic penguins, specifically a population of Chinstrap penguins (Pygoscelis antarctica) in the Vapour Col penguin rookery in Deception Island, South Shetlands, Antarctica. We collected peripheral blood from 50 adult and 30 chick Chinstrap penguins. Examination of t...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2016.02.006
更新日期：2016-04-01 00:00:00
