Abstract：
:Theileriosis is a disease of domesticated animals in tropical and subtropical countries and causes significant reductions in livestock productivity. The arid region of Punjab in Pakistan is notorious for the presence of the vector tick (Acari: Ixodidae) and tick-borne diseases, such as theileriosis and babesiosis. The distribution of Theileria annulata and T. orientalis in the Chakwal district of Punjab was determined by developing a multiplex recombinase polymerase amplification (RPA) assay as a scientific basis for formulating control strategies for bovine theileriosis. Specificity was evaluated using DNA from related piroplasm species, while analytical sensitivity was calculated using a long fragment of the enolase gene. A total of 188 blood samples were collected on FTA cards (Whatman®) from tick-infested asymptomatic breeds of cattle (Bos indicus, Bos taurus, and Bos indicus × Bos taurus) in the study district. Finally, infections with of T. annulata and T. orientalis were detected using the multiplex RPA and compared with the conventional multiplex polymerase chain reaction (PCR). The multiplex RPA specifically amplified 282-bp and 229-bp fragments of the enolase gene from T. annulata and T. orientalis and had no cross-reaction with other piroplasm species. It was determined that 45 (23.9%) and 5 (2.6%) out of 188 blood samples were positive for T. annulata and T. orientalis, respectively, when examined using RPA. Multiplex PCR detection indicated that 32 (17.0%) and 3 (1.6%) blood samples were positive for T. annulata and T. orientalis, respectively. In the present study, a specific RPA method was developed for simultaneous differentiation and detection of T. annulata and T. orientalis infections and used for the first time for the detection of the two bovine Theileria infections.
journal_name
Ticks Tick Borne Disjournal_title
Ticks and tick-borne diseasesauthors
Hassan MA,Liu J,Sajid MS,Rashid M,Mahmood A,Abbas Q,Guan G,Yin H,Luo Jdoi
10.1016/j.ttbdis.2018.03.028subject
Has Abstractpub_date
2018-05-01 00:00:00pages
1002-1005issue
4eissn
1877-959Xissn
1877-9603pii
S1877-959X(18)30004-9journal_volume
9pub_type
杂志文章abstract：:Complement is a key first line innate host defense system in the blood of vertebrates. Upon activation, this powerful defense mechanism can elicit inflammatory responses, lyse non-self-cells, or mark them for opsonophagocytic removal. Blood-feeding arthropods thus require the ability to block host complement activatio...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.101354
更新日期：2020-03-01 00:00:00
abstract：:Workers at veterinary clinics are exposed to zoonotic pathogens, which, though infrequently, can lead to serious consequences. The aim of the present study was to estimate the seroprevalence for rickettsial pathogens in veterinary clinic personnel in Ciudad Juárez, Chihuahua, Mexico. Voluntary participants included 10...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.10.012
更新日期：2019-02-01 00:00:00
abstract：:Hepatozoon canis is a blood parasite of the suborder Adeleorina infecting wild and domestic canids. Transmission occurs by oral uptake of Rhipicephalus sanguineus sensu lato vector ticks infected with H. canis, but vertical transmission is also assumed to be possible. In German foxes, a high prevalence of H. canis has...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2020.101520
更新日期：2020-11-01 00:00:00
abstract：:Basic knowledge of species, ecological preferences and behavior of ticks in preserved rainforests are scanty. We herein describe species richness, seasonal activity, altitude influence and questing height of ticks along 3375 m of animal trails within the largest remain of inland Atlantic rainforest in Brazil, the Igua...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2020.101471
更新日期：2020-09-01 00:00:00
abstract：:Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) ticks cause economic losses for cattle industries throughout tropical and subtropical regions of the world estimated at $US2.5 billion annually. Lack of access to efficacious long-lasting vaccination regimes and increases in tick acaricide resistance have led to th...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2014.03.005
更新日期：2014-09-01 00:00:00
abstract：:The current work evaluated road-killed Pampas foxes (Lycalopex gymnocercus) and their ticks for the presence of vector-borne agents in the ecoregion Esteros del Iberá in northeastern Argentina. Spleen, lung and blood samples and Amblyomma tigrinum adult ticks collected from the foxes were tested by polymerase chain re...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.101318
更新日期：2020-03-01 00:00:00
abstract：:Severe fever with thrombocytopenia syndrome virus (SFTSV) has been emerging and has caused many human cases in China, Japan and Korea. Some studies speculated that SFTSV was transmitted with bird migration among these countries. Notably, SFTS cases have been identified in a Chinese island named Dachen which is situate...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.02.009
更新日期：2019-04-01 00:00:00
abstract：:Thymol is a monoterpene present in plants of the families Lamiaceae, Verbenaceae and Apiaceae. Despite its proven acaricidal activity, little is known about the mechanism of action of thymol in ticks. Thus, the aim of this study was to perform a morpho-histochemical analysis of the synganglion and salivary glands of p...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.11.003
更新日期：2019-02-01 00:00:00
abstract：:Vector-borne pathogens are transmitted between vertebrate hosts and arthropod vectors, two immensely different environments for the pathogen. There is further differentiation among vertebrate hosts that often have complex, species-specific immunological responses to the pathogen. All this presents a heterogeneous envi...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2015.03.011
更新日期：2015-04-01 00:00:00
abstract：:Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potent...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.01.001
更新日期：2017-03-01 00:00:00
abstract：:North Asian tick-typhus (NATT), also known as Siberian tick typhus, is the main tick-borne rickettsiosis in Siberia, Russia. Recently, a fatal infection in a four-year-old girl with typical tick-borne rickettsiosis symptoms (fever, rash, eschar at the site of the tick bite, myalgia) and meningeal syndrome was register...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.101278
更新日期：2019-10-01 00:00:00
abstract：:Rodents are hosts of numerous pathogenic agents of public health importance globally. Their ability to harbor these pathogens without showing overt clinical signs of disease has epidemiologic consequences. In some rural settings in Nigeria, humans and rodents do not only share feeds and abode, but the latter may end u...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.10.004
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is increasingly emerging tool for identification of arthropods including tick vectors using whole or body part of specimens. The challenges of the present study were to assess MALDI-TOF MS profiling for the both ident...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2015.04.013
更新日期：2015-07-01 00:00:00
abstract：:The present study provides the first epidemiological data on infection with Babesia bovis in cattle raised in the southwestern Brazilian Amazon. Blood clot samples were filtered through nylon cloth before being submitted to DNA extraction. PCR and nested-PCR were applied to assess the frequency of infection with B. bo...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2012.08.001
更新日期：2013-02-01 00:00:00
abstract：:In North America, different strains of the Lyme disease-causing bacterium Borrelia burgdorferi sensu stricto cluster into phylogenetic groups that are associated with different levels of pathogenicity and, for some, specific rodent reservoir hosts. Here we explore whether landscape connectivity, by impacting host disp...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.07.001
更新日期：2018-09-01 00:00:00
abstract：:Rodents are important reservoir hosts of tick-borne pathogens. Anaplasma phagocytophilum is the causative agent of granulocytic anaplasmosis of both medical and veterinary importance. In Europe, this pathogen is primarily transmitted by the Ixodes ricinus tick among a wide range of vertebrate hosts. However, to what d...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2014.07.012
更新日期：2014-10-01 00:00:00
abstract：:All of the parasitic stages of Ixodes inopinatus n. sp. are described from specimens collected by flagging and on lizards and foxes. The new species replaces I. ricinus in dry areas of the Mediterranean region in Spain, Portugal, Morocco, Algeria and Tunisia. It has also been collected in areas of western Germany in s...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2014.05.003
更新日期：2014-10-01 00:00:00
abstract：:In the present study, the presence of tick-associated bacteria and protozoa in Ornithodoros rostratus ticks (adults, nymphs, and eggs) from the Pantanal region of Brazil were determined by molecular detection. In these ticks, DNA from protozoa in the genera Babesia and Hepatozoon, and bacteria from the genera Ricketts...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2012.02.003
更新日期：2012-09-01 00:00:00
abstract：:Surveillance indicates that tick-borne diseases are a common problem in the United States. Nevertheless, little is known regarding the experience or management practices of healthcare providers who treat these conditions. The purpose of the present study was to characterize the frequency of tick-borne diseases in clin...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2014.01.008
更新日期：2014-06-01 00:00:00
abstract：:The lone star tick, Amblyomma americanum (L.) (Acari: Ixodidae), is established in southeastern Nebraska yet the prevalence of tick-associated microorganisms is not known. An initial PCR-based analysis for Ehrlichia chaffeensis, Ehrlichia ewingii, and Borrelia infection in host-seeking adult ticks collected in southea...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2015.10.008
更新日期：2016-02-01 00:00:00
abstract：:The giant anteater (Myrmecophaga tridactyla) and the collared anteater (Tamandua tetradactyla) are widespread in Brazil and found in all Brazilian biomes. These hosts frequently use domestic animal environments such as pastures, where tick and related microorganism interchange may occur between hosts. Reports of tick ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.01.008
更新日期：2019-04-01 00:00:00
abstract：:Due to the ongoing expansion of Ixodes scapularis (blacklegged tick) throughout the northeastern and midwestern United States, there is need to identify the role wildlife hosts play in the establishment and maintenance of tick populations. To quantify and synthesize the patterns of I. scapularis and Borrelia burgdorfe...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.ttbdis.2018.04.006
更新日期：2018-07-01 00:00:00
abstract：:Rhipicephalus sanguineus sensu lato (s.l.) ticks act as intermediate host for a range of canine vector-borne pathogens, including nematodes ranked in the genus Cercopithifilaria. Though being the object of several studies in the last years, information on the distribution of these parasites is still lacking. In this s...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.04.007
更新日期：2017-06-01 00:00:00
abstract：:This study is the first report about the prevalence of tick-borne pathogens, as well as their (co-)infection rates, in the museum-archived I. ricinus female ticks collected in Moldova in 1960. A total of 16.7% (21/126) ticks was mono-infected. Borrelia burgdorferi sensu stricto was revealed as the most abundant specie...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2012.12.004
更新日期：2013-06-01 00:00:00
abstract：:Anaplasma phagocytophilum is an obligate intracellular and tick-transmitted bacterium, which causes granulocytic anaplasmosis in animals and humans. Although infection with A. phagocytophilum in domestic animals and vector ticks is documented, there is sparse information on the occurrence of A. phagocytophilum in wild...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2013.11.001
更新日期：2014-04-01 00:00:00
abstract：:Equine piroplasmosis is one of the most significant tick-borne disease of equids. The prevalence of this disease in donkeys of semi-arid Kenya remains largely unexplored. The primary objective of this study was to demonstrate the extent to which donkeys in Nuu division, Kenya have been exposed to the haemoprotozoans B...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2015.05.015
更新日期：2015-07-01 00:00:00
abstract：:Coxiella burnetii is the causative agent of Q fever. In order to explore the occurrence of C. burnetii in ticks, samples were collected from horses, dogs and humans living in a Cuban occidental community. The species most commonly recovered were Amblyomma mixtum (67%), Rhipicephalus sanguineus s.l. (27%) and Dermacent...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2015.08.008
更新日期：2016-02-01 00:00:00
abstract：:Tick-borne diseases have a complex epidemiology that depends on different ecological communities, associating several species of vertebrate hosts, vectors and pathogens. While most studies in Europe are focused on Ixodes ricinus, other Ixodes species may also be involved in the transmission or maintenance of pathogens...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2020.101625
更新日期：2021-03-01 00:00:00
abstract：:This study reports the results of a comparative test of identification of ticks occurring in Western Europe and Northern Africa. A total of 14 laboratories were voluntarily enrolled in the test. Each participant received between 22 and 25 specimens of adult and nymphal ticks of 11 species: Dermacentor marginatus, D. r...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.03.001
更新日期：2017-06-01 00:00:00
abstract：:Ticks collected from mustelids from four counties in Maine and one in New Hampshire were identified after harvest. Of the 18 fishers Martes pennanti Erxleben, two mink Neovison vison Schreber, and one long-tailed weasel Mustela frenata Lichtenstein, 589 ticks were collected and identified. They were identified as, in ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2014.02.001
更新日期：2014-06-01 00:00:00
