Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation.


:Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.


Ticks Tick Borne Dis


Mihaljica D,Marković D,Radulović Ž,Mulenga A,Ćakić S,Sukara R,Samardžić J,Tomanović S




Has Abstract


2017-03-01 00:00:00














  • Non-surgical management of spontaneous splenic rupture due to Babesia microti infection.

    abstract::Babesiosis is an emerging tick-borne infection. We describe 2 hospitalized patients from the United States with spontaneous splenic rupture attributable to Babesia microti infection. The first patient was a 36-year-old previously healthy man with a low-grade splenic rupture not requiring surgical intervention. The sec...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: El Khoury MY,Gandhi R,Dandache P,Lombardo G,Wormser GP

    更新日期:2011-12-01 00:00:00

  • A putative novel strain of Ehrlichia infecting Amblyomma tigrinum associated with Pampas fox (Lycalopex gymnocercus) in Esteros del Iberá ecoregion, Argentina.

    abstract::The current work evaluated road-killed Pampas foxes (Lycalopex gymnocercus) and their ticks for the presence of vector-borne agents in the ecoregion Esteros del Iberá in northeastern Argentina. Spleen, lung and blood samples and Amblyomma tigrinum adult ticks collected from the foxes were tested by polymerase chain re...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Eberhardt AT,Fernandez C,Fargnoli L,Beldomenico PM,Monje LD

    更新日期:2020-03-01 00:00:00

  • Rickettsia amblyommatis isolated from Amblyomma mixtum (Acari: Ixodida) from two sites in Panama.

    abstract::Rickettsia amblyommatis is widely distributed in the Americas, and has been reported to infect different species of ticks within its distribution. In Panama, R. amblyommatis is the most common Rickettsia and its presence was molecularly detected in nine species of ticks and one flea species. This work described the is...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Bermúdez C S,Zaldívar Y,Domínguez A L,Hernández M,de Antinori MEB,Krawczak FS

    更新日期:2021-01-01 00:00:00

  • Epidemiology and cost of hospital care for Lyme borreliosis in Germany: lessons from a health care utilization database analysis.

    abstract::To date, relatively little is known about the economic and medical impact of Lyme borreliosis (LB) on European health care systems, especially for the inpatient sector. This retrospective analysis is based on data provided for the years 2007-2011 by a German statutory health insurance company (DAK-Gesundheit) covering...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Lohr B,Müller I,Mai M,Norris DE,Schöffski O,Hunfeld KP

    更新日期:2015-02-01 00:00:00

  • Coxiella burnetii and Rickettsia conorii: Two zoonotic pathogens in peridomestic rodents and their ectoparasites in Nigeria.

    abstract::Rodents are hosts of numerous pathogenic agents of public health importance globally. Their ability to harbor these pathogens without showing overt clinical signs of disease has epidemiologic consequences. In some rural settings in Nigeria, humans and rodents do not only share feeds and abode, but the latter may end u...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Kamani J,Baneth G,Gutiérrez R,Nachum-Biala Y,Mumcuoglu KY,Harrus S

    更新日期:2018-01-01 00:00:00

  • Presence of Borrelia in different populations of Ixodes pararicinus from northwestern Argentina.

    abstract::This work was performed to evaluate the presence of Borrelia in different populations of Ixodes pararicinus from northwestern Argentina (Jujuy, Salta and Tucumán provinces). Questing adults and nymphs of I. pararicinus were collected from vegetation, and I. pararicinus nymphs were also collected on birds. Eighty-two t...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Saracho Bottero MN,Sebastian PS,Carvalho LA,Claps LG,Mastropaolo M,Mangold AJ,Venzal JM,Nava S

    更新日期:2017-06-01 00:00:00

  • Evidence for an effect of landscape connectivity on Borrelia burgdorferi sensu stricto dispersion in a zone of range expansion.

    abstract::In North America, different strains of the Lyme disease-causing bacterium Borrelia burgdorferi sensu stricto cluster into phylogenetic groups that are associated with different levels of pathogenicity and, for some, specific rodent reservoir hosts. Here we explore whether landscape connectivity, by impacting host disp...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Mechai S,Margos G,Feil EJ,Lindsay LR,Michel P,Kotchi SO,Ogden NH

    更新日期:2018-09-01 00:00:00

  • Molecular detection of Anaplasma marginale infection in carrier cattle.

    abstract::Anaplasma marginale infections are responsible for widespread morbidity and mortality particularly in crossbred and exotic breeds of cattle in the tropics and subtropics. In the present study, a semi-nested PCR assay was utilized for the detection of A. marginale infection in carrier cattle from different areas of Pun...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Singh H,Jyoti,Haque M,Singh NK,Rath SS

    更新日期:2012-02-01 00:00:00

  • Development of a serodiagnostic multi-species ELISA against tick-borne encephalitis virus using subviral particles.

    abstract::Tick-borne encephalitis virus (TBEV) is a zoonotic agent causing severe encephalitis in humans. A wide range of animal species could be infected with TBEV in endemic areas. A serological survey of wild animals is effective in identifying TBEV-endemic areas. Safe, simple, and reliable TBEV serodiagnostic tools are need...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Inagaki E,Sakai M,Hirano M,Muto M,Kobayashi S,Kariwa H,Yoshii K

    更新日期:2016-07-01 00:00:00

  • Serological and molecular detection of Theileria equi and Babesia caballi in Philippine horses.

    abstract::Theileria equi and Babesia caballi are tick-borne protozoan parasites that can cause anemia in horses. In the Philippines, serological detection of these parasites has only been reported in the Northern area (Luzon). In this study, 105 horses from Cebu and Bohol, Philippines were tested using peripheral blood smear ex...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Ybañez AP,Ybañez RHD,Talle MG,Arreglo RMT,Geens MJC,Villas JGI 3rd,Villar SR,Laruga CL,Cao S,Moumouni FPA,Liu M,Igarashi I,Xuan X

    更新日期:2018-07-01 00:00:00

  • Development of a quantitative real-time RT-PCR assay that differentiates between Kyasanur Forest disease virus and Alkhurma hemorrhagic fever virus.

    abstract::Kyasanur Forest disease virus (KFDV) and Alkhurma hemorrhagic fever virus (AHFV) are enveloped, positive-stranded RNA viruses of clinical importance with complex enzootic life cycles involving hematophagous ticks which feed on small and large mammals. Humans and monkeys are dead-end hosts for these viruses. Recent tre...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: N S,Hewson R,Afrough B,Bewley K,Arunkumar G

    更新日期:2020-05-01 00:00:00

  • First molecular detection of Rickettsia parkeri in Amblyomma tigrinum and Amblyomma dubitatum ticks from Uruguay.

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    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Lado P,Castro O,Labruna MB,Venzal JM

    更新日期:2014-10-01 00:00:00

  • Coxiella symbiont in the tick Ornithodoros rostratus (Acari: Argasidae).

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    pub_type: 杂志文章


    authors: Almeida AP,Marcili A,Leite RC,Nieri-Bastos FA,Domingues LN,Martins JR,Labruna MB

    更新日期:2012-09-01 00:00:00

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    authors: Wise LN,Kappmeyer LS,Silva MG,White SN,Grause JF,Knowles DP

    更新日期:2018-02-01 00:00:00

  • Thymol action on cells and tissues of the synganglia and salivary glands of Rhipicephalus sanguineus sensu lato females (Acari: Ixodidae).

    abstract::Thymol is a monoterpene present in plants of the families Lamiaceae, Verbenaceae and Apiaceae. Despite its proven acaricidal activity, little is known about the mechanism of action of thymol in ticks. Thus, the aim of this study was to perform a morpho-histochemical analysis of the synganglion and salivary glands of p...

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    pub_type: 杂志文章


    authors: Matos RS,Daemon E,de Oliveira Monteiro CM,Sampieri BR,Marchesini PBC,Delmonte C,Camargo-Mathias MI

    更新日期:2019-02-01 00:00:00

  • The first clinical and laboratory evidence of co-infection by Anaplasma phagocytophilum and Ehrlichia canis in a Brazilian dog.

    abstract::Information on Anaplasma phagocytophilum in Brazil is very restricted. The aim of this study was to report clinical, parasitological, hematological and molecular evidence of a natural A. phagocytophilum infection of an urban Brazilian dog. The dog was an eight-month-old male French bulldog. Veterinary clinical examina...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Silveira JA,Valente PC,Paes PR,Vasconcelos AV,Silvestre BT,Ribeiro MF

    更新日期:2015-04-01 00:00:00

  • Towards a genomics approach to tick (Acari: Ixodidae) control in cattle: a review.

    abstract::Ticks and tick-borne disease (TBD) are major challenges to cattle production in the tropics and subtropics. Economic losses associated with ticks amount to billions of dollars annually. Although efforts to eradicate ticks and TBD using chemical control strategies have been implemented in many developing countries for ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章,评审


    authors: Mapholi NO,Marufu MC,Maiwashe A,Banga CB,Muchenje V,MacNeil MD,Chimonyo M,Dzama K

    更新日期:2014-09-01 00:00:00

  • A surrogate life cycle of Amblyomma ovale Koch, 1844.

    abstract::As ticks spend most of their time off-host, the environment is a major selective force of these parasites. In fact, human impact on landscapes has favored a minority of tick species which became well-known pests. However, this is an ongoing process and novel pests may arise. We herein report a surrogate life cycle of ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Szabó MP,Martins TF,Nieri-Bastos FA,Spolidorio MG,Labruna MB

    更新日期:2012-09-01 00:00:00

  • Demonstration of lumpy skin disease virus infection in Amblyomma hebraeum and Rhipicephalus appendiculatus ticks using immunohistochemistry.

    abstract::Lumpy skin disease (LSD) is caused by lumpy skin disease virus (LSDV), a member of the genus Capripoxvirus. Transmission of the virus has been associated with haematophagous insects such as Stomoxys calcitrans as well as Aedes and Culex species of mosquitoes. Recent studies have reported the transmission of the virus ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Lubinga JC,Clift SJ,Tuppurainen ES,Stoltsz WH,Babiuk S,Coetzer JA,Venter EH

    更新日期:2014-03-01 00:00:00

  • Collection of immature Dermacentor reticulatus (Fabricius, 1794) ticks from vegetation and detection of Rickettsia raoultii in them.

    abstract::It is commonly assumed that Dermacentor reticulatus immature life stages are nidicolous and therefore cannot be collected from vegetation. However, in June and July of 2018 and 2019, a total of 47 questing D. reticulatus larvae and two nymphs were collected by the flagging method in two different sites close to the ci...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Schmuck HM,Chitimia-Dobler L,Król N,Kacza J,Pfeffer M

    更新日期:2020-11-01 00:00:00

  • Differences in prevalence of Borrelia burgdorferi and Anaplasma spp. infection among host-seeking Dermacentor occidentalis, Ixodes pacificus, and Ornithodoros coriaceus ticks in northwestern California.

    abstract::Previous studies revealed that the Pacific Coast tick (Dermacentor occidentalis) is infected occasionally with the agents of Lyme disease (Borrelia burgdorferi) or human granulocytic anaplasmosis (Anaplasma phagocytophilum) and that it is an inefficient experimental vector of B. burgdorferi. The relationship of the pa...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Lane RS,Mun J,Peribáñez MA,Fedorova N

    更新日期:2010-12-01 00:00:00

  • Tick-borne diseases in the Union of the Comoros are a hindrance to livestock development: Circulation and associated risk factors.

    abstract::Tick-borne diseases (TBD) occur in many temperate countries and are economically important in most tropical and subtropical areas, affecting dairy and beef cattle, as well as small ruminants. Four major tick-borne diseases have been detected in eastern and southern Africa: East Coast fever (ECF) caused by Theileria pa...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Boucher F,Moutroifi Y,Peba B,Ali M,Moindjie Y,Ruget AS,Abdouroihamane S,Madi Kassim A,Soulé M,Charafouddine O,Cêtre-Sossah C,Cardinale E

    更新日期:2020-01-01 00:00:00

  • A life stage-targeted acaricide application approach for the control of Haemaphysalis longicornis.

    abstract::Haemaphysalis longicornis Neumann is an invasive tick species that has recently been detected in the eastern United States. We designed field studies to monitor the population dynamics of H. longicornis over a year (2019) in New Jersey, and to assess the efficacy of a pyrethroid acaricide (lambda-cyhalothrin) in contr...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Bickerton M,McSorley K,Toledo A

    更新日期:2021-01-01 00:00:00

  • Lyme borreliae prevalence and genospecies distribution in ticks removed from humans.

    abstract::Lyme borreliosis (LB) is the most important human tick-borne disease, but Borrelia genospecies cause different clinical manifestations. Ticks of the genus Ixodes removed from humans between 2006 and 2012 were analysed for Borrelia burgdorferi sensu lato (sl) infections. The majority of ticks originated from the Greate...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Waindok P,Schicht S,Fingerle V,Strube C

    更新日期:2017-08-01 00:00:00

  • An integrative approach to understanding pyrethroid resistance in Rhipicephalus microplus and R. decoloratus ticks.

    abstract::Rhipicephalus microplus and Rhipicephalus decoloratus species occur in regions with savannah and temperate climates, typically in grassland and wooded areas used as cattle pasture. Both species are associated with the transmission of Anaplasma and Babesia spp., impacting livestock health and quality of livestock-assoc...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Wyk RDj,Baron S,Maritz-Olivier C

    更新日期:2016-06-01 00:00:00

  • Divergence of the TRP36 protein (gp36) in Ehrlichia canis strains found in Brazil.

    abstract::The molecular characterization of 16S rRNA gene and immunoreactive proteins of Ehrlichia canis usually provide little information about the overall diversity of this organism. On the other hand, distinct sequences of the Tandem Repeat Protein 36 (TRP36/gp36) gene of E. canis have been reported, indicating substantial ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 信件


    authors: Aguiar DM,Melo AL

    更新日期:2015-03-01 00:00:00

  • Prevalence of Anaplasma phagocytophilum in small rodents in France.

    abstract::Anaplasma phagocytophilum is an emerging zoonotic tick-borne pathogen affecting a wide range of mammals. Rodents are suspected to be natural reservoirs for this bacterium, but their role in the epidemiologic cycles affecting domestic animals and wild ungulates has not been demonstrated. This study aimed to improve our...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Chastagner A,Moinet M,Perez G,Roy E,McCoy KD,Plantard O,Agoulon A,Bastian S,Butet A,Rantier Y,Verheyden H,Cèbe N,Leblond A,Vourc'h G

    更新日期:2016-07-01 00:00:00

  • Detection of a Borrelia species in questing Gulf Coast ticks, Amblyomma maculatum.

    abstract::Borrelia spp. are agents of Lyme disease and relapsing fever, diseases which use Ixodes hard ticks and Ornithodoros soft ticks, respectively, as primary vectors. Some relapsing fever spirochetes, such as B. miyamotoi, are also found in hard ticks. To date, no Borrelia sp. is known to use the hard tick, Amblyomma macul...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Lee JK,Smith WC,McIntosh C,Ferrari FG,Moore-Henderson B,Varela-Stokes A

    更新日期:2014-06-01 00:00:00

  • Diversity of Coxiella-like and Francisella-like endosymbionts, and Rickettsia spp., Coxiella burnetii as pathogens in the tick populations of Slovakia, Central Europe.

    abstract::Ticks are important vectors of pathogens affecting humans and animals worldwide. They do not only carry pathogens but diverse commensal and symbiotic microorganisms are also present in ticks. A molecular screening for tick-borne pathogens and endosymbionts was carried out in Ixodes ricinus, Dermacentor reticulatus and...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Špitalská E,Sparagano O,Stanko M,Schwarzová K,Špitalský Z,Škultéty Ľ,Havlíková SF

    更新日期:2018-07-01 00:00:00

  • Diversity of two Theileria parva CD8+ antigens in cattle and buffalo-derived parasites in Tanzania.

    abstract::Theileria parva is a tick-transmitted protozoan parasite that causes a disease called East Coast fever (ECF) in cattle. This important tick borne-disease (TBD) causes significant economic losses in cattle in many sub-Saharan countries, including Tanzania. Cattle immunization using Muguga cocktail has been recommended ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Kerario II,Chenyambuga SW,Mwega ED,Rukambile E,Simulundu E,Simuunza MC

    更新日期:2019-08-01 00:00:00