Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation.

Abstract:

:Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.

journal_name

Ticks Tick Borne Dis

authors

Mihaljica D,Marković D,Radulović Ž,Mulenga A,Ćakić S,Sukara R,Samardžić J,Tomanović S

doi

10.1016/j.ttbdis.2017.01.001

subject

Has Abstract

pub_date

2017-03-01 00:00:00

pages

391-395

issue

3

eissn

1877-959X

issn

1877-9603

pii

S1877-959X(17)30009-2

journal_volume

8

pub_type

杂志文章
  • Vector-borne pathogens in ticks and EDTA-blood samples collected from client-owned dogs, Kiev, Ukraine.

    abstract::Due to the availability of adequate habitats in urban environments, e.g. city parks and recreational green areas, ticks from such settings may also carry pathogens of veterinary and public health concern. Thus, tick-borne infections may readily be identified in companion animals residing in urbanised areas. To investi...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2012.08.005

    authors: Hamel D,Silaghi C,Zapadynska S,Kudrin A,Pfister K

    更新日期:2013-02-01 00:00:00

  • Prevalence of Anaplasma phagocytophilum in small rodents in France.

    abstract::Anaplasma phagocytophilum is an emerging zoonotic tick-borne pathogen affecting a wide range of mammals. Rodents are suspected to be natural reservoirs for this bacterium, but their role in the epidemiologic cycles affecting domestic animals and wild ungulates has not been demonstrated. This study aimed to improve our...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2016.05.005

    authors: Chastagner A,Moinet M,Perez G,Roy E,McCoy KD,Plantard O,Agoulon A,Bastian S,Butet A,Rantier Y,Verheyden H,Cèbe N,Leblond A,Vourc'h G

    更新日期:2016-07-01 00:00:00

  • Toxic effects of various solvents against Rhipicephalus (Boophilus) annulatus.

    abstract::The current need of identification of a new acaricidal agent which is acceptable to public as environmentally safe is a daring task. Use of herbal acaricides is such an alternative. Most of the herbal extracts or fractions are dissolved in polar or non-polar solvents or detergents before tested for acaricidal activity...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2011.04.001

    authors: Ravindran R,Juliet S,Kumar KG,Sunil AR,Nair SN,Amithamol KK,Rawat AK,Ghosh S

    更新日期:2011-09-01 00:00:00

  • Associations between Ixodes scapularis ticks and small mammal hosts in a newly endemic zone in southeastern Canada: implications for Borrelia burgdorferi transmission.

    abstract::Immature Ixodes scapularis infestation and Borrelia burgdorferi infection of wild small mammals were studied from June to October in 2007 and from May to October in 2008 at 71 study sites in a zone where I. scapularis populations and environmental Lyme disease risk are emerging in southwestern Quebec. Seasonal host-se...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2011.03.005

    authors: Bouchard C,Beauchamp G,Nguon S,Trudel L,Milord F,Lindsay LR,Bélanger D,Ogden NH

    更新日期:2011-12-01 00:00:00

  • Non-surgical management of spontaneous splenic rupture due to Babesia microti infection.

    abstract::Babesiosis is an emerging tick-borne infection. We describe 2 hospitalized patients from the United States with spontaneous splenic rupture attributable to Babesia microti infection. The first patient was a 36-year-old previously healthy man with a low-grade splenic rupture not requiring surgical intervention. The sec...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2011.08.001

    authors: El Khoury MY,Gandhi R,Dandache P,Lombardo G,Wormser GP

    更新日期:2011-12-01 00:00:00

  • Tick (Ixodes ricinus) abundance and seasonality at recreational sites in the UK: hazards in relation to fine-scale habitat types revealed by complementary sampling methods.

    abstract::The seasonal risk to humans of picking up Ixodes ricinus ticks in different habitats at 3 recreational sites in the UK was assessed. A comprehensive range of vegetation types was sampled at 3-weekly intervals for 2 years, using standard blanket-dragging complemented by woollen leggings and square 'heel flags'. Ticks w...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2011.03.002

    authors: Dobson AD,Taylor JL,Randolph SE

    更新日期:2011-06-01 00:00:00

  • U.S. healthcare providers' experience with Lyme and other tick-borne diseases.

    abstract::Surveillance indicates that tick-borne diseases are a common problem in the United States. Nevertheless, little is known regarding the experience or management practices of healthcare providers who treat these conditions. The purpose of the present study was to characterize the frequency of tick-borne diseases in clin...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2014.01.008

    authors: Brett ME,Hinckley AF,Zielinski-Gutierrez EC,Mead PS

    更新日期:2014-06-01 00:00:00

  • Development of a quantitative real-time RT-PCR assay that differentiates between Kyasanur Forest disease virus and Alkhurma hemorrhagic fever virus.

    abstract::Kyasanur Forest disease virus (KFDV) and Alkhurma hemorrhagic fever virus (AHFV) are enveloped, positive-stranded RNA viruses of clinical importance with complex enzootic life cycles involving hematophagous ticks which feed on small and large mammals. Humans and monkeys are dead-end hosts for these viruses. Recent tre...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2020.101381

    authors: N S,Hewson R,Afrough B,Bewley K,Arunkumar G

    更新日期:2020-05-01 00:00:00

  • Molecular detection of Anaplasma marginale infection in carrier cattle.

    abstract::Anaplasma marginale infections are responsible for widespread morbidity and mortality particularly in crossbred and exotic breeds of cattle in the tropics and subtropics. In the present study, a semi-nested PCR assay was utilized for the detection of A. marginale infection in carrier cattle from different areas of Pun...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2011.10.002

    authors: Singh H,Jyoti,Haque M,Singh NK,Rath SS

    更新日期:2012-02-01 00:00:00

  • Lyme borreliae prevalence and genospecies distribution in ticks removed from humans.

    abstract::Lyme borreliosis (LB) is the most important human tick-borne disease, but Borrelia genospecies cause different clinical manifestations. Ticks of the genus Ixodes removed from humans between 2006 and 2012 were analysed for Borrelia burgdorferi sensu lato (sl) infections. The majority of ticks originated from the Greate...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2017.05.003

    authors: Waindok P,Schicht S,Fingerle V,Strube C

    更新日期:2017-08-01 00:00:00

  • Infection of Ixodes spp. tick cells with different Anaplasma phagocytophilum isolates induces the inhibition of apoptotic cell death.

    abstract::Anaplasma phagocytophilum is an intracellular rickettsial pathogen transmitted by Ixodes spp. ticks, which causes granulocytic anaplasmosis in humans, horses and dogs and tick-borne fever (TBF) in ruminants. In the United States, human granulocytic anaplasmosis (HGA) is highly prevalent while TBF has not been reported...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2015.07.001

    authors: Alberdi P,Ayllón N,Cabezas-Cruz A,Bell-Sakyi L,Zweygarth E,Stuen S,de la Fuente J

    更新日期:2015-09-01 00:00:00

  • Evidence for an effect of landscape connectivity on Borrelia burgdorferi sensu stricto dispersion in a zone of range expansion.

    abstract::In North America, different strains of the Lyme disease-causing bacterium Borrelia burgdorferi sensu stricto cluster into phylogenetic groups that are associated with different levels of pathogenicity and, for some, specific rodent reservoir hosts. Here we explore whether landscape connectivity, by impacting host disp...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2018.07.001

    authors: Mechai S,Margos G,Feil EJ,Lindsay LR,Michel P,Kotchi SO,Ogden NH

    更新日期:2018-09-01 00:00:00

  • Records of ticks (Acari: Ixodidae) on humans and distribution of spotted-fever cases and its tick vectors in Paraná State, southern Brazil.

    abstract::Brazilian spotted fever (BSF) is the deadliest rickettsiosis in the world. Although the epidemiology of the disease has been established in Brazil, there are still limited data available on distribution of tick vectors and tick species parasitizing humans in the country, particularly in Paraná State. The State of Para...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2020.101510

    authors: Valente JDM,Silva PW,Arzua M,Barros-Battesti DM,Martins TF,Silva AM,Vieira TSWJ,Labruna MB,Vieira RFC

    更新日期:2020-11-01 00:00:00

  • Development of an efficient antitick natural formulation for the control of acaricide-resistant ticks on livestock.

    abstract::Animal production has a key role in global economic development and food security. Ticks, specifically Rhipicephalus microplus cause substantial economic and health impacts on more than eighty percent of the world cattle population. Though synthetic acaricides play a major role in tick management, their injudicious us...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2021.101655

    authors: Shanmuganath C,Kumar S,Singh R,Sharma AK,Saminathan M,Saini M,Chigure G,Fular A,Kumar R,Juliet S,Upadhaya D,Kumar B,Srivastava S,Ghosh S

    更新日期:2021-01-19 00:00:00

  • Protozoan and bacterial pathogens in tick salivary glands in wild and domestic animal environments in South Africa.

    abstract::A total of 7364 ticks belonging to 13 species was collected from 64 game animals (belonging to 11 species) and from 64 livestock animals (cattle and sheep) living in close vicinity at 6 localities in 3 South African Provinces (Free State, Mpumalanga, and Limpopo). The geographic distribution of all tick species was co...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2013.10.003

    authors: Berggoetz M,Schmid M,Ston D,Wyss V,Chevillon C,Pretorius AM,Gern L

    更新日期:2014-03-01 00:00:00

  • Dissociating effect of salivary gland extract from Ixodes ricinus on human fibroblasts: Potential impact on Borrelia transmission.

    abstract::Understanding the mechanism of pathogen transmission is essential for the development of strategies to reduce arthropod-borne diseases. The pharmaco- and immunomodulatory properties of insect and acarine saliva play an essential role in the efficiency of pathogen transmission. The skin as the site where arthropod sali...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2018.12.005

    authors: Boeuf A,Schnell G,Bernard Q,Kern A,Westermann B,Ehret-Sabatier L,Grillon A,Schramm F,Jaulhac B,Boulanger N

    更新日期:2019-02-01 00:00:00

  • Babesia microti thioredoxin 3 is an effective antioxidant and involved in the response to antiprotozoal drugs.

    abstract::The intra-erythrocytic apicomplexan Babesia microti is the predominant pathogen that causes human babesiosis, an infectious disease that occurs worldwide. B. microti relies on the antioxidant including thioredoxin system to maintain the redox balance during the erythrocytic stage. In the present study, the full-length...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2018.01.021

    authors: Huang J,Xiong K,Zhang H,Zhao Y,Cao J,Zhou Y,Gong H,Zhou J

    更新日期:2018-03-01 00:00:00

  • Molecular detection of tick-borne pathogens in Ixodes ricinus from Moldova collected in 1960.

    abstract::This study is the first report about the prevalence of tick-borne pathogens, as well as their (co-)infection rates, in the museum-archived I. ricinus female ticks collected in Moldova in 1960. A total of 16.7% (21/126) ticks was mono-infected. Borrelia burgdorferi sensu stricto was revealed as the most abundant specie...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2012.12.004

    authors: Movila A,Toderas I,Uspenskaia I,Conovalov J

    更新日期:2013-06-01 00:00:00

  • The role of Ixodes scapularis, Borrelia burgdorferi and wildlife hosts in Lyme disease prevalence: A quantitative review.

    abstract::Due to the ongoing expansion of Ixodes scapularis (blacklegged tick) throughout the northeastern and midwestern United States, there is need to identify the role wildlife hosts play in the establishment and maintenance of tick populations. To quantify and synthesize the patterns of I. scapularis and Borrelia burgdorfe...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章,meta分析,评审

    doi:10.1016/j.ttbdis.2018.04.006

    authors: Halsey SJ,Allan BF,Miller JR

    更新日期:2018-07-01 00:00:00

  • Spatio-temporal variation of tick-borne encephalitis (TBE) incidence in the Czech Republic: is the current explanation of the disease's rise satisfactory?

    abstract::A marked increase in tick-borne encephalitis (TBE) incidence has been observed in Europe during the last 2 decades. Hypothetical causes include global climatic fluctuations, human-induced environmental changes, and socio-economic changes. These factors are thought to be disproportionately relevant in different geograp...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2010.05.003

    authors: Zeman P,Pazdiora P,Benes C

    更新日期:2010-09-01 00:00:00

  • Note on Ehrlichia chaffeensis, Ehrlichia ewingii, and "Borrelia lonestari" infection in lone star ticks (Acari: Ixodidae), Nebraska, USA.

    abstract::The lone star tick, Amblyomma americanum (L.) (Acari: Ixodidae), is established in southeastern Nebraska yet the prevalence of tick-associated microorganisms is not known. An initial PCR-based analysis for Ehrlichia chaffeensis, Ehrlichia ewingii, and Borrelia infection in host-seeking adult ticks collected in southea...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2015.10.008

    authors: Maegli A,Loy JD,Cortinas R

    更新日期:2016-02-01 00:00:00

  • Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis.

    abstract::With the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2016.11.008

    authors: Giglioti R,Oliveira HN,Ibelli AMG,Bilhassi TB,Néo TA,Santana CH,Rabelo MD,Machado RZ,de Souza Chagas AC,de Sena Oliveira MC

    更新日期:2017-03-01 00:00:00

  • The first clinical and laboratory evidence of co-infection by Anaplasma phagocytophilum and Ehrlichia canis in a Brazilian dog.

    abstract::Information on Anaplasma phagocytophilum in Brazil is very restricted. The aim of this study was to report clinical, parasitological, hematological and molecular evidence of a natural A. phagocytophilum infection of an urban Brazilian dog. The dog was an eight-month-old male French bulldog. Veterinary clinical examina...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2015.01.003

    authors: Silveira JA,Valente PC,Paes PR,Vasconcelos AV,Silvestre BT,Ribeiro MF

    更新日期:2015-04-01 00:00:00

  • Tick-borne disease preventive practices and perceptions in an endemic area.

    abstract::Lyme disease is the most commonly reported vector-borne illness in the United States. Since the institution of Nationally Notifiable surveillance efforts for Lyme disease in the United States in 1991, there has been a consistent increase in the number of reported cases. Thus, the need for targeted prevention strategie...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2015.12.003

    authors: Butler AD,Sedghi T,Petrini JR,Ahmadi R

    更新日期:2016-03-01 00:00:00

  • A field survey for the seroprevalence of Theileria equi and Babesia caballi in donkeys from Nuu Division, Kenya.

    abstract::Equine piroplasmosis is one of the most significant tick-borne disease of equids. The prevalence of this disease in donkeys of semi-arid Kenya remains largely unexplored. The primary objective of this study was to demonstrate the extent to which donkeys in Nuu division, Kenya have been exposed to the haemoprotozoans B...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2015.05.015

    authors: Oduori DO,Onyango SC,Kimari JN,MacLeod ET

    更新日期:2015-07-01 00:00:00

  • Probability of Rhipicephalus microplus introduction into farms by cattle movement using a Bayesian Belief Network.

    abstract::Attempts to eliminate Rhipicephalus microplus from Uruguay have been unsuccessful, and, currently, the country is divided into two areas: a tick-free area and a tick-infested area. In the tick-infested area, different farms face different situations. Some farms are in regions where, due to environmental conditions or ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2019.04.009

    authors: Miraballes C,Riet-Correa F,Saporiti T,Lara S,Parodi P,Sanchez J

    更新日期:2019-06-01 00:00:00

  • Occupational risk for personnel working in veterinary clinics through exposure to vectors of rickettsial pathogens.

    abstract::Workers at veterinary clinics are exposed to zoonotic pathogens, which, though infrequently, can lead to serious consequences. The aim of the present study was to estimate the seroprevalence for rickettsial pathogens in veterinary clinic personnel in Ciudad Juárez, Chihuahua, Mexico. Voluntary participants included 10...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2018.10.012

    authors: Escárcega-Ávila AM,de la Mora-Covarrubias A,Quezada-Casasola A,Jiménez-Vega F

    更新日期:2019-02-01 00:00:00

  • A fatal case of tick-borne rickettsiosis caused by mixed Rickettsia sibirica subsp. sibirica and "Candidatus Rickettsia tarasevichiae" infection in Russia.

    abstract::North Asian tick-typhus (NATT), also known as Siberian tick typhus, is the main tick-borne rickettsiosis in Siberia, Russia. Recently, a fatal infection in a four-year-old girl with typical tick-borne rickettsiosis symptoms (fever, rash, eschar at the site of the tick bite, myalgia) and meningeal syndrome was register...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2019.101278

    authors: Rudakov N,Samoylenko I,Shtrek S,Igolkina Y,Rar V,Zhirakovskaia E,Tkachev S,Kostrykina T,Blokhina I,Lentz P,Tikunova N

    更新日期:2019-10-01 00:00:00

  • Genotyping acaricide resistance profiles of Rhipicephalus microplus tick populations from communal land areas of Zimbabwe.

    abstract::Acaricide resistance is one of the greatest threats towards the successful control of vector ticks worldwide. Communal farmers of Zimbabwe use amitraz as the most common acaricide with occasional usage of pyrethroids and organophosphates. As a strategy towards developing an effective acaricide resistance management sy...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2017.10.017

    authors: Sungirai M,Baron S,Moyo DZ,De Clercq P,Maritz-Olivier C,Madder M

    更新日期:2018-01-01 00:00:00

  • A comparative test of ixodid tick identification by a network of European researchers.

    abstract::This study reports the results of a comparative test of identification of ticks occurring in Western Europe and Northern Africa. A total of 14 laboratories were voluntarily enrolled in the test. Each participant received between 22 and 25 specimens of adult and nymphal ticks of 11 species: Dermacentor marginatus, D. r...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2017.03.001

    authors: Estrada-Peña A,D'Amico G,Palomar AM,Dupraz M,Fonville M,Heylen D,Habela MA,Hornok S,Lempereur L,Madder M,Núncio MS,Otranto D,Pfaffle M,Plantard O,Santos-Silva MM,Sprong H,Vatansever Z,Vial L,Mihalca AD

    更新日期:2017-06-01 00:00:00