Abstract:
:Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) ticks cause economic losses for cattle industries throughout tropical and subtropical regions of the world estimated at $US2.5 billion annually. Lack of access to efficacious long-lasting vaccination regimes and increases in tick acaricide resistance have led to the investigation of targets for the development of novel tick vaccines and treatments. In vitro tick feeding has been used for many tick species to study the effect of new acaricides on the transmission of tick-borne pathogens. Few studies have reported the use of in vitro feeding for functional genomic studies using RNA interference and/or the effect of specific anti-tick antibodies. In particular, in vitro feeding reports for the cattle tick are limited due to its relatively short hypostome. Previously published methods were further modified to broaden optimal tick sizes/weights, feeding sources including bovine and ovine serum, optimisation of commercially available blood anti-coagulant tubes, and IgG concentrations for effective antibody delivery. Ticks are fed overnight and monitored for ∼5-6 weeks to determine egg output and success of larval emergence using a humidified incubator. Lithium-heparin blood tubes provided the most reliable anti-coagulant for bovine blood feeding compared with commercial citrated (CPDA) and EDTA tubes. Although >30mg semi-engorged ticks fed more reliably, ticks as small as 15mg also fed to repletion to lay viable eggs. Ticks which gained less than ∼10mg during in vitro feeding typically did not lay eggs. One mg/ml IgG from Bm86-vaccinated cattle produced a potent anti-tick effect in vitro (83% efficacy) similar to that observed in vivo. Alternatively, feeding of dsRNA targeting Bm86 did not demonstrate anti-tick effects (11% efficacy) compared with the potent effects of ubiquitin dsRNA. This study optimises R. microplus tick in vitro feeding methods which support the development of cattle tick vaccines and treatments.
journal_name
Ticks Tick Borne Disjournal_title
Ticks and tick-borne diseasesauthors
Lew-Tabor AE,Bruyeres AG,Zhang B,Rodriguez Valle Mdoi
10.1016/j.ttbdis.2014.03.005subject
Has Abstractpub_date
2014-09-01 00:00:00pages
500-10issue
5eissn
1877-959Xissn
1877-9603pii
S1877-959X(14)00064-8journal_volume
5pub_type
杂志文章abstract::Acaricide resistance is one of the greatest threats to sustainable and effective control of vector ticks worldwide. The amitraz resistance status in cattle tick, Rhipicephalus microplus populations collected from 18 districts of Punjab in north-western India were characterized using bioassay and molecular assays. The ...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2020.101578
更新日期:2021-01-01 00:00:00
abstract::'Candidatus Rickettsia amblyommii' is a spotted fever group rickettsia that is not considered pathogenic, although there is serologic evidence of possible infection in animals and humans. The aim of this study was to evaluate the pathogenic potential of a Costa Rican strain of 'Candidatus R. amblyommii' in guinea pigs...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2015.07.008
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abstract::Rickettsia rickettsii is the agent of Brazilian spotted fever (BSF), the most lethal tick-borne disease in the western hemisphere. Amblyomma sculptum is the main vector of R. rickettsii in southeastern Brazil. Capybaras act as primary hosts for this tick species, and as amplifying hosts for R. rickettsii, creating new...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2020.101463
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abstract::This work was performed to evaluate the presence of Borrelia in different populations of Ixodes pararicinus from northwestern Argentina (Jujuy, Salta and Tucumán provinces). Questing adults and nymphs of I. pararicinus were collected from vegetation, and I. pararicinus nymphs were also collected on birds. Eighty-two t...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2017.02.008
更新日期:2017-06-01 00:00:00
abstract::Haemaphysalis longicornis Neumann is an invasive tick species that has recently been detected in the eastern United States. We designed field studies to monitor the population dynamics of H. longicornis over a year (2019) in New Jersey, and to assess the efficacy of a pyrethroid acaricide (lambda-cyhalothrin) in contr...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2020.101581
更新日期:2021-01-01 00:00:00
abstract::Theileriosis is a disease of domesticated animals in tropical and subtropical countries and causes significant reductions in livestock productivity. The arid region of Punjab in Pakistan is notorious for the presence of the vector tick (Acari: Ixodidae) and tick-borne diseases, such as theileriosis and babesiosis. The...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2018.03.028
更新日期:2018-05-01 00:00:00
abstract::A cross-sectional study combining different serological and molecular techniques for the detection of Ehrlichia species in dogs and their ticks was carried out with data from all regions of Costa Rica. A seroprevalence of 32.1% (131/408), and infection with E. canis of 3.2% (13/407) was found, whereas 6.9% (9/130) of ...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2016.07.006
更新日期:2016-10-01 00:00:00
abstract::Vector-borne pathogens are transmitted between vertebrate hosts and arthropod vectors, two immensely different environments for the pathogen. There is further differentiation among vertebrate hosts that often have complex, species-specific immunological responses to the pathogen. All this presents a heterogeneous envi...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2015.03.011
更新日期:2015-04-01 00:00:00
abstract::This study investigated the first outbreak of oriental theileriosis in a herd of beef cattle in South Australia using a newly established multiplexed tandem PCR (MT-PCR) to identify, differentiate and quantitate the four genotypes (buffeli, chitose, ikeda and type 5) of Theileria orientalis recognised to occur in Aust...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2015.04.012
更新日期:2015-07-01 00:00:00
abstract::Rickettsia amblyommatis is widely distributed in the Americas, and has been reported to infect different species of ticks within its distribution. In Panama, R. amblyommatis is the most common Rickettsia and its presence was molecularly detected in nine species of ticks and one flea species. This work described the is...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2020.101597
更新日期:2021-01-01 00:00:00
abstract::Tick-borne pathogens (TBPs) are common in livestock of sub-Saharan Africa. However, information regarding TBPs in sheep and goats in Sudan is limited. In this study, 178 blood samples of sheep and goats in Blue Nile and West Kordofan states were investigated for TBPs using PCR. Overall, 110 (61.8%) samples were found ...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2018.01.014
更新日期:2018-03-01 00:00:00
abstract::A total of 7364 ticks belonging to 13 species was collected from 64 game animals (belonging to 11 species) and from 64 livestock animals (cattle and sheep) living in close vicinity at 6 localities in 3 South African Provinces (Free State, Mpumalanga, and Limpopo). The geographic distribution of all tick species was co...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2013.10.003
更新日期:2014-03-01 00:00:00
abstract::The hard tick Ixodes ricinus is the principal vector of Lyme borreliosis (LB) group spirochaetes in Europe, but it also transmits a large number of other microbial pathogens that are of importance to animal and human health. Here, we characterise geographically distinct populations of this important ectoparasite based...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2013.10.001
更新日期:2014-03-01 00:00:00
abstract::The seasonal risk to humans of picking up Ixodes ricinus ticks in different habitats at 3 recreational sites in the UK was assessed. A comprehensive range of vegetation types was sampled at 3-weekly intervals for 2 years, using standard blanket-dragging complemented by woollen leggings and square 'heel flags'. Ticks w...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2011.03.002
更新日期:2011-06-01 00:00:00
abstract::Anaplasma phagocytophilum is an intracellular rickettsial pathogen transmitted by Ixodes spp. ticks, which causes granulocytic anaplasmosis in humans, horses and dogs and tick-borne fever (TBF) in ruminants. In the United States, human granulocytic anaplasmosis (HGA) is highly prevalent while TBF has not been reported...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2015.07.001
更新日期:2015-09-01 00:00:00
abstract::Animal production has a key role in global economic development and food security. Ticks, specifically Rhipicephalus microplus cause substantial economic and health impacts on more than eighty percent of the world cattle population. Though synthetic acaricides play a major role in tick management, their injudicious us...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2021.101655
更新日期:2021-01-19 00:00:00
abstract::In this study, we examine the current pattern of tick diversity and host use in French Guiana, South America, from 97 sampling localities encompassing peri-urban, rural and natural habitats. We collected 3395 ticks, including 1485 specimens from 45 vertebrate species (humans, domestic and wild animals) and 1910 questi...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2018.09.003
更新日期:2019-01-01 00:00:00
abstract::Ixodes affinis Neumann (1899) and Ixodes scapularis Say (1821) are tick vectors of the etiologic agent of Lyme disease, Borrelia burgdorferi sensu stricto. Ixodes affinis and I. scapularis are morphologically very similar, and as they are sympatric in the mid- and south-Atlantic U.S. coastal states, their accurate ide...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2013.08.003
更新日期:2014-02-01 00:00:00
abstract::Canine hepatozoonosis is a tick-borne protozoal disease caused by Hepatozoon spp. Two species of Hepatozoon are currently known to infect dogs as Hepatozoon canis and H. americanum. Although H. canis generally causes a chronic infection with relatively mild clinical alterations compared to H. americanum, infection by ...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2015.03.004
更新日期:2015-04-01 00:00:00
abstract::Previous studies revealed that the Pacific Coast tick (Dermacentor occidentalis) is infected occasionally with the agents of Lyme disease (Borrelia burgdorferi) or human granulocytic anaplasmosis (Anaplasma phagocytophilum) and that it is an inefficient experimental vector of B. burgdorferi. The relationship of the pa...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2010.09.004
更新日期:2010-12-01 00:00:00
abstract::Lumpy skin disease (LSD) is caused by lumpy skin disease virus (LSDV), a member of the genus Capripoxvirus. Transmission of the virus has been associated with haematophagous insects such as Stomoxys calcitrans as well as Aedes and Culex species of mosquitoes. Recent studies have reported the transmission of the virus ...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2013.09.010
更新日期:2014-03-01 00:00:00
abstract::This is the first reported case of Babesia sp. in Antarctic penguins, specifically a population of Chinstrap penguins (Pygoscelis antarctica) in the Vapour Col penguin rookery in Deception Island, South Shetlands, Antarctica. We collected peripheral blood from 50 adult and 30 chick Chinstrap penguins. Examination of t...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2016.02.006
更新日期:2016-04-01 00:00:00
abstract::Anaplasma phagocytophilum is an emerging zoonotic tick-borne pathogen affecting a wide range of mammals. Rodents are suspected to be natural reservoirs for this bacterium, but their role in the epidemiologic cycles affecting domestic animals and wild ungulates has not been demonstrated. This study aimed to improve our...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2016.05.005
更新日期:2016-07-01 00:00:00
abstract::Kyasanur Forest disease virus (KFDV) and Alkhurma hemorrhagic fever virus (AHFV) are enveloped, positive-stranded RNA viruses of clinical importance with complex enzootic life cycles involving hematophagous ticks which feed on small and large mammals. Humans and monkeys are dead-end hosts for these viruses. Recent tre...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2020.101381
更新日期:2020-05-01 00:00:00
abstract::Ehrlichiosis is a tick-borne bacterial disease caused by pathogens of the Ehrlichia genus. Although human ehrlichiosis has not been reported in Japan, Ehrlichia spp., which are closely related to Ehrlichia chaffeensis, were detected in several species of ixodid ticks. In this study, the presence of Ehrlichia spp. in t...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2018.11.010
更新日期:2019-02-01 00:00:00
abstract::Lyme disease is the most commonly reported vector-borne illness in the United States. Since the institution of Nationally Notifiable surveillance efforts for Lyme disease in the United States in 1991, there has been a consistent increase in the number of reported cases. Thus, the need for targeted prevention strategie...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2015.12.003
更新日期:2016-03-01 00:00:00
abstract::Only a few reported cases indicate that Rickettsia helvetica and Rickettsia monacensis can cause disease in humans. Exposure to these two spotted fever group (SFG) rickettsiae occurs through bites of Ixodes ricinus, also the primary vector of Lyme borreliosis in Europe. To date, it is unclear how often exposure to the...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2015.12.010
更新日期:2016-03-01 00:00:00
abstract::Lyme borreliosis (LB) is the most frequent vector-borne disease in Germany. For more than 10 years, data from mandatory notifications have been available from 6 federal states in the eastern part of Germany. A common case definition was applied. Clinical manifestations of erythema migrans, neuroborreliosis (radiculone...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2013.10.010
更新日期:2014-04-01 00:00:00
abstract::Cytauxzoonosis is an emerging, tick-transmitted, protozoan disease affecting domestic and wild felids and caused by Cytauxzoon felis, Cytauxzoon manul and Cytauxzoon spp. This study aimed to determine the presence of infection with Cytauxzoon spp. in Felis silvestris silvestris in Italy, in order to enhance the compre...
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2016.04.003
更新日期:2016-07-01 00:00:00
abstract::Anaplasma phagocytophilum, transmitted by ticks of the genus Ixodes, was first described in Scotland as the agent of tick-borne fever in sheep and more recently as the cause of human granulocytic anaplasmosis in the U.S. and Europe. We previously reported sheep as an experimental host for the human NY-18 isolate of A....
journal_title:Ticks and tick-borne diseases
pub_type: 杂志文章
doi:10.1016/j.ttbdis.2014.05.014
更新日期:2014-10-01 00:00:00