当前位置： SCI文献检索 > Ticks and Tick-Borne Diseases期刊下所有文献 > Studies of Anaplasma phagocytophilum in sheep experimentally infected with the human NY-18 isolate: characterization of tick feeding sites.

Studies of Anaplasma phagocytophilum in sheep experimentally infected with the human NY-18 isolate: characterization of tick feeding sites.

Abstract：

:Anaplasma phagocytophilum, transmitted by ticks of the genus Ixodes, was first described in Scotland as the agent of tick-borne fever in sheep and more recently as the cause of human granulocytic anaplasmosis in the U.S. and Europe. We previously reported sheep as an experimental host for the human NY-18 isolate of A. phagocytophilum. While clinical signs were not observed and infected granulocytes were not seen in stained blood smears, these sheep served as a good host for infection of ticks. In this research we characterized tick feeding sites to better understand tick/host/pathogen interactions. Ixodes scapularis adults were allowed to feed for 2 and 4 days on experimentally infected sheep, after which biopsies were taken beneath tick feeding sites for histopathology, PCR and immunohistochemistry (IHC) studies. In addition, the expression of selected immune response genes was studied in blood and feeding site biopsies. While necrosis was too advanced in 4-day biopsies for accurate cell counts, higher numbers of eosinophils and neutrophils were found in 2-day biopsies from infected sheep as compared with the uninfected controls. An unexpected result was the documentation of higher dermal inflammation in infected sheep at sites without ticks. A. phagocytophilum infected granulocytes were localized by immunohistochemistry (IHC) in skin biopsies using rabbit antibodies against the recombinant A. phagocytophilum major surface protein 4 as the primary antibody for indirect peroxidase-anti-peroxidase and fluorescent antibody IHC. These infected cells are likely to be the source of infection for ticks. Sheep therefore served as good hosts for studying host/pathogen/tick interactions of this human strain of A. phagocytophilum, and provided a means of producing infected ticks for future studies on tick/pathogen developmental and transmission cycles.

journal_name

Ticks Tick Borne Dis

journal_title

Ticks and tick-borne diseases

authors

Reppert E,Galindo RC,Ayllón N,Breshears MA,Kocan KM,Blouin EF,de la Fuente J

doi

10.1016/j.ttbdis.2014.05.014

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

744-52

issue

6

eissn

1877-959X

issn

1877-9603

pii

S1877-959X(14)00150-2

journal_volume

5

pub_type

杂志文章

相关文献

Ticks and Tick-Borne Diseases文献大全
  • Detection of a Borrelia species in questing Gulf Coast ticks, Amblyomma maculatum.

    abstract：:Borrelia spp. are agents of Lyme disease and relapsing fever, diseases which use Ixodes hard ticks and Ornithodoros soft ticks, respectively, as primary vectors. Some relapsing fever spirochetes, such as B. miyamotoi, are also found in hard ticks. To date, no Borrelia sp. is known to use the hard tick, Amblyomma macul...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2014.02.004

    authors： Lee JK,Smith WC,McIntosh C,Ferrari FG,Moore-Henderson B,Varela-Stokes A

    更新日期：2014-06-01 00:00:00

  • Seasonal activity patterns of the western black-legged tick, Ixodes pacificus, in relation to onset of human Lyme disease in northwestern California.

    abstract：:Seasonal activity patterns of questing western black-legged ticks, Ixodes pacificus were investigated in northwestern California. Adult I. pacificus became active in the fall (late October/early November) and their appearance was associated with the first rain of the season. Following a peak in January, the abundance ...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2014.05.002

    authors： Salkeld DJ,Castro MB,Bonilla D,Kjemtrup A,Kramer VL,Lane RS,Padgett KA

    更新日期：2014-10-01 00:00:00

  • Molecular identification and evaluation of Coxiella-like endosymbionts genetic diversity carried by cattle ticks in Algeria.

    abstract：:Coxiella-like bacteria are a large group of yet-to-isolate and characterize bacteria phylogenetically close to the agent of Q fever, Coxiella burnetii, and often associated with ixodid ticks worldwide. This study was designed to assess the presence of Coxiella-like endosymbionts (CLE) in ticks and to describe their ge...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2020.101493

    authors： Rahal M,Medkour H,Diarra AZ,Bitam I,Parola P,Mediannikov O

    更新日期：2020-09-01 00:00:00

  • The role of Ixodes scapularis, Borrelia burgdorferi and wildlife hosts in Lyme disease prevalence: A quantitative review.

    abstract：:Due to the ongoing expansion of Ixodes scapularis (blacklegged tick) throughout the northeastern and midwestern United States, there is need to identify the role wildlife hosts play in the establishment and maintenance of tick populations. To quantify and synthesize the patterns of I. scapularis and Borrelia burgdorfe...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.ttbdis.2018.04.006

    authors： Halsey SJ,Allan BF,Miller JR

    更新日期：2018-07-01 00:00:00

  • Brazilian spotted fever: real-time PCR for diagnosis of fatal cases.

    abstract：:Suspicion of Brazilian spotted fever (BSF) should occur in endemic regions upon surveillance of the acute febrile icteric hemorrhagic syndrome (AFIHS). However, limitations associated with currently available laboratory tests pose a challenge to early diagnosis, especially in fatal cases. Two real-time PCR (qPCR) prot...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2012.10.027

    authors： dos Santos FC,do Nascimento EM,Katz G,Angerami RN,Colombo S,de Souza ER,Labruna MB,da Silva MV

    更新日期：2012-12-01 00:00:00

  • The occurrence of Anaplasmataceae in European populations of invasive carnivores.

    abstract：:The raccoon (Procyon lotor) and the raccoon dog (Nyctereutes procyonoides) belong to a group of the invasive species. The introduced species as potential reservoirs for vector-borne pathogens have been the subject of recent research, though there are still no data with reference to the European population of the racco...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2018.03.018

    authors： Hildebrand J,Buńkowska-Gawlik K,Adamczyk M,Gajda E,Merta D,Popiołek M,Perec-Matysiak A

    更新日期：2018-05-01 00:00:00

  • Presence of Rickettsia aeschlimannii, 'Candidatus Rickettsia barbariae' and Coxiella burnetii in ticks from livestock in Northwestern Algeria.

    abstract：:Livestock and their ectoparasites are involved in the epidemiology of several zoonotic diseases. Studies regarding the molecular detection of infectious agents in ticks from Northwestern Algeria are scarce. Thus, the presence of spotted fever group Rickettsia spp., Anaplasmataceae microorganisms and Coxiella burnetii ...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2019.04.018

    authors： Abdelkadir K,Palomar AM,Portillo A,Oteo JA,Ait-Oudhia K,Khelef D

    更新日期：2019-06-01 00:00:00

  • Associations between Ixodes scapularis ticks and small mammal hosts in a newly endemic zone in southeastern Canada: implications for Borrelia burgdorferi transmission.

    abstract：:Immature Ixodes scapularis infestation and Borrelia burgdorferi infection of wild small mammals were studied from June to October in 2007 and from May to October in 2008 at 71 study sites in a zone where I. scapularis populations and environmental Lyme disease risk are emerging in southwestern Quebec. Seasonal host-se...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2011.03.005

    authors： Bouchard C,Beauchamp G,Nguon S,Trudel L,Milord F,Lindsay LR,Bélanger D,Ogden NH

    更新日期：2011-12-01 00:00:00

  • Comparative genetic diversity of Lyme disease bacteria in Northern Californian ticks and their vertebrate hosts.

    abstract：:Vector-borne pathogens are transmitted between vertebrate hosts and arthropod vectors, two immensely different environments for the pathogen. There is further differentiation among vertebrate hosts that often have complex, species-specific immunological responses to the pathogen. All this presents a heterogeneous envi...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2015.03.011

    authors： Swei A,Bowie VC,Bowie RC

    更新日期：2015-04-01 00:00:00

  • Babesia odocoilei and zoonotic pathogens identified from Ixodes scapularis ticks in southern Ontario, Canada.

    abstract：:Cervid babesiosis, caused by the protozoan hemoparasite Babesia odocoilei and transmitted by the blacklegged tick Ixodes scapularis, is an emerging disease of Canadian cervids. This pathogen has not yet been described in humans. Data are lacking on the role of migratory birds in the adventitious spread of Ba. odocoile...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2019.02.016

    authors： Milnes EL,Thornton G,Léveillé AN,Delnatte P,Barta JR,Smith DA,Nemeth N

    更新日期：2019-04-01 00:00:00

  • Genotyping acaricide resistance profiles of Rhipicephalus microplus tick populations from communal land areas of Zimbabwe.

    abstract：:Acaricide resistance is one of the greatest threats towards the successful control of vector ticks worldwide. Communal farmers of Zimbabwe use amitraz as the most common acaricide with occasional usage of pyrethroids and organophosphates. As a strategy towards developing an effective acaricide resistance management sy...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2017.10.017

    authors： Sungirai M,Baron S,Moyo DZ,De Clercq P,Maritz-Olivier C,Madder M

    更新日期：2018-01-01 00:00:00

  • Borrelia spp. in ticks and birds from a protected urban area in Buenos Aires city, Argentina.

    abstract：:This study was aimed to know epidemiological aspects of Borrelia spp. in a protected urban area of Buenos Aires city, Argentina, where thousands of people visit this area for recreational purposes. Ticks were collected from vegetation, birds and dogs. Three hundred and forty birds belonging to 43 species, 41 genera, 1...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2019.101282

    authors： Cicuttin GL,De Salvo MN,Venzal JM,Nava S

    更新日期：2019-10-01 00:00:00

  • A fatal case of tick-borne rickettsiosis caused by mixed Rickettsia sibirica subsp. sibirica and "Candidatus Rickettsia tarasevichiae" infection in Russia.

    abstract：:North Asian tick-typhus (NATT), also known as Siberian tick typhus, is the main tick-borne rickettsiosis in Siberia, Russia. Recently, a fatal infection in a four-year-old girl with typical tick-borne rickettsiosis symptoms (fever, rash, eschar at the site of the tick bite, myalgia) and meningeal syndrome was register...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2019.101278

    authors： Rudakov N,Samoylenko I,Shtrek S,Igolkina Y,Rar V,Zhirakovskaia E,Tkachev S,Kostrykina T,Blokhina I,Lentz P,Tikunova N

    更新日期：2019-10-01 00:00:00

  • An instance of parasitism on a human by a nymph of the kangaroo soft tick, Ornithodoros gurneyi Warburton, 1926 (Acari: Argasidae) in South Australia.

    abstract：:The kangaroo soft tick, Ornithodoros gurneyi Warburton, 1926, is a poorly studied argasid of the more arid regions of Australia. Anecdotal accounts have stated that this tick species will bite humans, and yet bites have not been described. Herein we report on an instance of parasitism by a nymph of O. gurneyi on a hum...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2020.101632

    authors： Norval G,Sharrad RD,Ross KE,Gardner MG

    更新日期：2021-03-01 00:00:00

  • Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation.

    abstract：:Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potent...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2017.01.001

    authors： Mihaljica D,Marković D,Radulović Ž,Mulenga A,Ćakić S,Sukara R,Samardžić J,Tomanović S

    更新日期：2017-03-01 00:00:00

  • Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis.

    abstract：:With the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2016.11.008

    authors： Giglioti R,Oliveira HN,Ibelli AMG,Bilhassi TB,Néo TA,Santana CH,Rabelo MD,Machado RZ,de Souza Chagas AC,de Sena Oliveira MC

    更新日期：2017-03-01 00:00:00

  • Description of all the stages of Ixodes inopinatus n. sp. (Acari: Ixodidae).

    abstract：:All of the parasitic stages of Ixodes inopinatus n. sp. are described from specimens collected by flagging and on lizards and foxes. The new species replaces I. ricinus in dry areas of the Mediterranean region in Spain, Portugal, Morocco, Algeria and Tunisia. It has also been collected in areas of western Germany in s...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2014.05.003

    authors： Estrada-Peña A,Nava S,Petney T

    更新日期：2014-10-01 00:00:00

  • First detection of Cytauxzoon spp. infection in European wildcats (Felis silvestris silvestris) of Italy.

    abstract：:Cytauxzoonosis is an emerging, tick-transmitted, protozoan disease affecting domestic and wild felids and caused by Cytauxzoon felis, Cytauxzoon manul and Cytauxzoon spp. This study aimed to determine the presence of infection with Cytauxzoon spp. in Felis silvestris silvestris in Italy, in order to enhance the compre...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2016.04.003

    authors： Veronesi F,Ravagnan S,Cerquetella M,Carli E,Olivieri E,Santoro A,Pesaro S,Berardi S,Rossi G,Ragni B,Beraldo P,Capelli G

    更新日期：2016-07-01 00:00:00

  • Population dynamics of multiple symbionts in the hard tick, Dermacentor silvarum Olenev (Acari: Ixodidae).

    abstract：:Previously, we reported that Coxiella-like, Rickettsia-like and Arsenophonus-like symbionts could simultaneously coexist in Dermacentor silvarum. In this study, we examined their burdens and population dynamics in a single host during the host life cycle using quantitative PCR. Our results showed that multiple symbion...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2015.10.002

    authors： Liu L,Li L,Liu J,Yu Z,Yang X,Liu J

    更新日期：2016-02-01 00:00:00

  • Molecular evidence for the inhibition of cytochrome p450s and cholinesterases in ticks by the repellent DEET.

    abstract：:For more than 50 years DEET (N,N-Diethyl-m-toluamide) has been considered the gold standard of repellents. It is applied to the skin or clothing to deter mosquitoes and other blood-sucking invertebrate pests from approaching and/or settling, and ultimately it provides temporary protection from bites. Despite rampant g...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2018.12.006

    authors： Koloski CW,LeMoine CMR,Klonowski AR,Smith CM,Cassone BJ

    更新日期：2019-04-01 00:00:00

  • Protozoan and bacterial pathogens in tick salivary glands in wild and domestic animal environments in South Africa.

    abstract：:A total of 7364 ticks belonging to 13 species was collected from 64 game animals (belonging to 11 species) and from 64 livestock animals (cattle and sheep) living in close vicinity at 6 localities in 3 South African Provinces (Free State, Mpumalanga, and Limpopo). The geographic distribution of all tick species was co...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2013.10.003

    authors： Berggoetz M,Schmid M,Ston D,Wyss V,Chevillon C,Pretorius AM,Gern L

    更新日期：2014-03-01 00:00:00

  • Detection of Rickettsia helvetica and Candidatus R. tarasevichiae DNA in Ixodes persulcatus ticks collected in Northeastern European Russia (Komi Republic).

    abstract：:The number of tick-borne infections in the northern European regions of Russia has increased considerably in the last years. In the present study, 676 unfed adult Ixodes persulcatus ticks were collected in the Komi Republic from 2011 to 2013 to study tick-borne rickettsioses. Rickettsia spp. DNA was detected by PCR in...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2017.04.001

    authors： Kartashov MY,Glushkova LI,Mikryukova TP,Korabelnikov IV,Egorova YI,Tupota NL,Protopopova EV,Konovalova SN,Ternovoi VA,Loktev VB

    更新日期：2017-06-01 00:00:00

  • Anaplasma marginale outer membrane protein vaccine candidates are conserved in North American and South African strains.

    abstract：:Bovine anaplasmosis is a globally economically important tick-borne disease caused by the obligate intraerythrocytic rickettsia, Anaplasma marginale. A live Anaplasma centrale blood-based vaccine is available, but it does not protect against all A. marginale field strains and may also transmit other blood-borne pathog...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2020.101444

    authors： Hove P,Brayton KA,Liebenberg J,Pretorius A,Oosthuizen MC,Noh SM,Collins NE

    更新日期：2020-07-01 00:00:00

  • Serological and molecular evidence for spotted fever group Rickettsia and Borrelia burgdorferi sensu lato co-infections in The Netherlands.

    abstract：:Only a few reported cases indicate that Rickettsia helvetica and Rickettsia monacensis can cause disease in humans. Exposure to these two spotted fever group (SFG) rickettsiae occurs through bites of Ixodes ricinus, also the primary vector of Lyme borreliosis in Europe. To date, it is unclear how often exposure to the...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2015.12.010

    authors： Koetsveld J,Tijsse-Klasen E,Herremans T,Hovius JW,Sprong H

    更新日期：2016-03-01 00:00:00

  • Rickettsia amblyommatis isolated from Amblyomma mixtum (Acari: Ixodida) from two sites in Panama.

    abstract：:Rickettsia amblyommatis is widely distributed in the Americas, and has been reported to infect different species of ticks within its distribution. In Panama, R. amblyommatis is the most common Rickettsia and its presence was molecularly detected in nine species of ticks and one flea species. This work described the is...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2020.101597

    authors： Bermúdez C S,Zaldívar Y,Domínguez A L,Hernández M,de Antinori MEB,Krawczak FS

    更新日期：2021-01-01 00:00:00

  • Parasitism of mustelids by ixodid ticks (Acari: Ixodidae), Maine and New Hampshire, U.S.A.

    abstract：:Ticks collected from mustelids from four counties in Maine and one in New Hampshire were identified after harvest. Of the 18 fishers Martes pennanti Erxleben, two mink Neovison vison Schreber, and one long-tailed weasel Mustela frenata Lichtenstein, 589 ticks were collected and identified. They were identified as, in ...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2014.02.001

    authors： Lubelczyk C,Lacombe EH,Elias SP,Beati L,Rand PW,Smith RP Jr

    更新日期：2014-06-01 00:00:00

  • Molecular detection of tick-borne pathogens in Ixodes ricinus from Moldova collected in 1960.

    abstract：:This study is the first report about the prevalence of tick-borne pathogens, as well as their (co-)infection rates, in the museum-archived I. ricinus female ticks collected in Moldova in 1960. A total of 16.7% (21/126) ticks was mono-infected. Borrelia burgdorferi sensu stricto was revealed as the most abundant specie...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2012.12.004

    authors： Movila A,Toderas I,Uspenskaia I,Conovalov J

    更新日期：2013-06-01 00:00:00

  • Detection of human bacterial pathogens in ticks collected from Louisiana black bears (Ursus americanus luteolus).

    abstract：:There are 4 major human-biting tick species in the northeastern United States, which include: Amblyomma americanum, Amblyomma maculatum, Dermacentor variabilis, and Ixodes scapularis. The black bear is a large mammal that has been shown to be parasitized by all the aforementioned ticks. We investigated the bacterial i...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2012.12.002

    authors： Leydet BF Jr,Liang FT

    更新日期：2013-04-01 00:00:00

  • Probability of Rhipicephalus microplus introduction into farms by cattle movement using a Bayesian Belief Network.

    abstract：:Attempts to eliminate Rhipicephalus microplus from Uruguay have been unsuccessful, and, currently, the country is divided into two areas: a tick-free area and a tick-infested area. In the tick-infested area, different farms face different situations. Some farms are in regions where, due to environmental conditions or ...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2019.04.009

    authors： Miraballes C,Riet-Correa F,Saporiti T,Lara S,Parodi P,Sanchez J

    更新日期：2019-06-01 00:00:00

  • An individual-based model for the dispersal of Ixodes scapularis by ovenbirds and wood thrushes during fall migration.

    abstract：:Ixodes scapularis is responsible for the transmission of a variety of pathogens in North America, including Borrelia burgdorferi sensu stricto, Anaplasma phagocytophilum and Babesia microti. Songbirds have previously been described as agents of tick dispersal, and a combination of empirical data and modeling efforts h...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2019.05.012

    authors： Tonelli BA,Dearborn DC

    更新日期：2019-08-01 00:00:00