当前位置： SCI文献检索 > Ticks and Tick-Borne Diseases期刊下所有文献 > Identification of tick species and disseminate pathogen using hemolymph by MALDI-TOF MS.

Identification of tick species and disseminate pathogen using hemolymph by MALDI-TOF MS.

Abstract：

BACKGROUND:Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is increasingly emerging tool for identification of arthropods including tick vectors using whole or body part of specimens. The challenges of the present study were to assess MALDI-TOF MS profiling for the both identification of tick species and Rickettsia spp. in infected ticks using hemolymph as protein mixture. METHODS:Firstly, hemolymph protein mixture from legs of 5 tick species, Rhipicephalus sanguineus, Rhipicephalus bursa, Dermacentor marginatus, Hyalomma marginatum rufipes and Amblyomma variegatum infected by Rickettsia africae were submitted to MALDI-TOF MS to assess tick species identification ability. Secondly, hemolymph MS spectra from Rh. sanguineus infected or not by Rickettsia c. conorii were compared to detect protein profiles changes. Finally, leg hemolymph MS spectra from new specimens of the 5 tick species were tested blindly including ticks infected by R. c. conorii. Discriminating mass peaks distinguishing the R. c. conorii infected and non-infected Rh sanguineus were determined. RESULTS:Consistent and reproducible MS profiles were obtained into each tick species. Comparison of MS spectra revealed distinct hemolymph protein profiles according to tick species. MS spectra changes were observed between hemolymphs from R. c. conorii-infected and non-infected Rh. sanguineus specimens, revealing 17 discriminating mass peaks. Clustering analysis based on MS protein profiles highlighted that hemolymph samples were grouped according to tick species. All tick hemolymph samples blindly tested against our home-made arthropod MS reference database were correctly identified at the species distinguishing also R. c. conorii-infected from Rickettsia-free Rh. sanguineus specimens. CONCLUSION:The present study demonstrated the use of hemolymph MS profiles for dual identification of tick species and associated pathogens. This concomitant identification could be helpful for tick entomological diagnosis, notably for specimens removed directly on patients.

journal_name

Ticks Tick Borne Dis

journal_title

Ticks and tick-borne diseases

authors

Yssouf A,Almeras L,Berenger JM,Laroche M,Raoult D,Parola P

doi

10.1016/j.ttbdis.2015.04.013

subject

Has Abstract

pub_date

2015-07-01 00:00:00

pages

579-86

issue

5

eissn

1877-959X

issn

1877-9603

pii

S1877-959X(15)00081-3

journal_volume

6

pub_type

杂志文章

相关文献

Ticks and Tick-Borne Diseases文献大全
  • Seroprevalence of severe fever with thrombocytopenia syndrome virus antibodies among inhabitants of Dachen Island, eastern China.

    abstract：:Severe fever with thrombocytopenia syndrome virus (SFTSV) has been emerging and has caused many human cases in China, Japan and Korea. Some studies speculated that SFTSV was transmitted with bird migration among these countries. Notably, SFTS cases have been identified in a Chinese island named Dachen which is situate...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2019.02.009

    authors： Shen W,Lin H,Weng J,Hu Y,Liu Y,Li J,Xu F,Guan C,Sun J

    更新日期：2019-04-01 00:00:00

  • Parasitism of mustelids by ixodid ticks (Acari: Ixodidae), Maine and New Hampshire, U.S.A.

    abstract：:Ticks collected from mustelids from four counties in Maine and one in New Hampshire were identified after harvest. Of the 18 fishers Martes pennanti Erxleben, two mink Neovison vison Schreber, and one long-tailed weasel Mustela frenata Lichtenstein, 589 ticks were collected and identified. They were identified as, in ...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2014.02.001

    authors： Lubelczyk C,Lacombe EH,Elias SP,Beati L,Rand PW,Smith RP Jr

    更新日期：2014-06-01 00:00:00

  • Response to letter regarding article "How far north are migrant birds transporting the tick Ixodes scapularis in Canada? Insights from stable hydrogen isotope analyses of feathers".

    abstract：:In this letter we respond to correspondence and clarify the intent and importance of our article. ...

    journal_title：Ticks and tick-borne diseases

    pub_type： 评论,信件

    doi：10.1016/j.ttbdis.2015.12.002

    authors： Ogden NH,Barker IK,Francis CM,Heagy A,Lindsay LR,Hobson KA

    更新日期：2016-03-01 00:00:00

  • Detection of Rickettsia helvetica and Candidatus R. tarasevichiae DNA in Ixodes persulcatus ticks collected in Northeastern European Russia (Komi Republic).

    abstract：:The number of tick-borne infections in the northern European regions of Russia has increased considerably in the last years. In the present study, 676 unfed adult Ixodes persulcatus ticks were collected in the Komi Republic from 2011 to 2013 to study tick-borne rickettsioses. Rickettsia spp. DNA was detected by PCR in...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2017.04.001

    authors： Kartashov MY,Glushkova LI,Mikryukova TP,Korabelnikov IV,Egorova YI,Tupota NL,Protopopova EV,Konovalova SN,Ternovoi VA,Loktev VB

    更新日期：2017-06-01 00:00:00

  • A putative novel strain of Ehrlichia infecting Amblyomma tigrinum associated with Pampas fox (Lycalopex gymnocercus) in Esteros del Iberá ecoregion, Argentina.

    abstract：:The current work evaluated road-killed Pampas foxes (Lycalopex gymnocercus) and their ticks for the presence of vector-borne agents in the ecoregion Esteros del Iberá in northeastern Argentina. Spleen, lung and blood samples and Amblyomma tigrinum adult ticks collected from the foxes were tested by polymerase chain re...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2019.101318

    authors： Eberhardt AT,Fernandez C,Fargnoli L,Beldomenico PM,Monje LD

    更新日期：2020-03-01 00:00:00

  • An individual-based model for the dispersal of Ixodes scapularis by ovenbirds and wood thrushes during fall migration.

    abstract：:Ixodes scapularis is responsible for the transmission of a variety of pathogens in North America, including Borrelia burgdorferi sensu stricto, Anaplasma phagocytophilum and Babesia microti. Songbirds have previously been described as agents of tick dispersal, and a combination of empirical data and modeling efforts h...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2019.05.012

    authors： Tonelli BA,Dearborn DC

    更新日期：2019-08-01 00:00:00

  • Multilocus sequence typing using mitochondrial genes (mtMLST) reveals geographic population structure of Ixodes ricinus ticks.

    abstract：:The hard tick Ixodes ricinus is the principal vector of Lyme borreliosis (LB) group spirochaetes in Europe, but it also transmits a large number of other microbial pathogens that are of importance to animal and human health. Here, we characterise geographically distinct populations of this important ectoparasite based...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2013.10.001

    authors： Dinnis RE,Seelig F,Bormane A,Donaghy M,Vollmer SA,Feil EJ,Kurtenbach K,Margos G

    更新日期：2014-03-01 00:00:00

  • Investigating the first outbreak of oriental theileriosis in cattle in South Australia using multiplexed tandem PCR (MT-PCR).

    abstract：:This study investigated the first outbreak of oriental theileriosis in a herd of beef cattle in South Australia using a newly established multiplexed tandem PCR (MT-PCR) to identify, differentiate and quantitate the four genotypes (buffeli, chitose, ikeda and type 5) of Theileria orientalis recognised to occur in Aust...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2015.04.012

    authors： Gebrekidan H,Gasser RB,Perera PK,McGrath S,McGrath S,Stevenson MA,Jabbar A

    更新日期：2015-07-01 00:00:00

  • First molecular detection and characterization of tick-borne pathogens in water buffaloes in Bohol, Philippines.

    abstract：:The water buffalo industry is a vital part of the Philippine livestock economy and is an essential contributor to the developing local dairy industry. Although relatively less susceptible to diseases, water buffaloes can still be infected and can act as reservoirs of tick-borne pathogens (TBPs). However, limited infor...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2019.03.016

    authors： Galon EMS,Adjou Moumouni PF,Ybañez RHD,Ringo AE,Efstratiou A,Lee SH,Liu M,Guo H,Gao Y,Li J,Salces CB,Maurillo BCA,Boldbaatar D,Ybañez AP,Xuan X

    更新日期：2019-06-01 00:00:00

  • Isolation and molecular detection of Ehrlichia species from ticks in western, central, and eastern Japan.

    abstract：:Ehrlichiosis is a tick-borne bacterial disease caused by pathogens of the Ehrlichia genus. Although human ehrlichiosis has not been reported in Japan, Ehrlichia spp., which are closely related to Ehrlichia chaffeensis, were detected in several species of ixodid ticks. In this study, the presence of Ehrlichia spp. in t...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2018.11.010

    authors： Taira M,Ando S,Kawabata H,Fujita H,Kadosaka T,Sato H,Monma N,Ohashi N,Saijo M

    更新日期：2019-02-01 00:00:00

  • Phylogenetic analysis of the mitochondrial genomes and nuclear rRNA genes of ticks reveals a deep phylogenetic structure within the genus Haemaphysalis and further elucidates the polyphyly of the genus Amblyomma with respect to Amblyomma sphenodonti and A

    abstract：:We sequenced the entire mitochondrial genomes of 3 species of metastriate ticks: Haemaphysalis formosensis, H. parva, and Amblyomma cajennense. We also sequenced two thirds (ca. 9500bp) of the mitochondrial genomes of H. humerosa and H. hystricis. We used these 5 mitochondrial genome sequences together with the 13 tic...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2013.02.002

    authors： Burger TD,Shao R,Barker SC

    更新日期：2013-06-01 00:00:00

  • Vector-borne pathogens in ticks and EDTA-blood samples collected from client-owned dogs, Kiev, Ukraine.

    abstract：:Due to the availability of adequate habitats in urban environments, e.g. city parks and recreational green areas, ticks from such settings may also carry pathogens of veterinary and public health concern. Thus, tick-borne infections may readily be identified in companion animals residing in urbanised areas. To investi...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2012.08.005

    authors： Hamel D,Silaghi C,Zapadynska S,Kudrin A,Pfister K

    更新日期：2013-02-01 00:00:00

  • Serology, molecular detection and risk factors of Ehrlichia canis infection in dogs in Costa Rica.

    abstract：:A cross-sectional study combining different serological and molecular techniques for the detection of Ehrlichia species in dogs and their ticks was carried out with data from all regions of Costa Rica. A seroprevalence of 32.1% (131/408), and infection with E. canis of 3.2% (13/407) was found, whereas 6.9% (9/130) of ...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2016.07.006

    authors： Barrantes-González AV,Jiménez-Rocha AE,Romero-Zuñiga JJ,Dolz G

    更新日期：2016-10-01 00:00:00

  • Studies of Anaplasma phagocytophilum in sheep experimentally infected with the human NY-18 isolate: characterization of tick feeding sites.

    abstract：:Anaplasma phagocytophilum, transmitted by ticks of the genus Ixodes, was first described in Scotland as the agent of tick-borne fever in sheep and more recently as the cause of human granulocytic anaplasmosis in the U.S. and Europe. We previously reported sheep as an experimental host for the human NY-18 isolate of A....

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2014.05.014

    authors： Reppert E,Galindo RC,Ayllón N,Breshears MA,Kocan KM,Blouin EF,de la Fuente J

    更新日期：2014-10-01 00:00:00

  • Molecular identification and evaluation of Coxiella-like endosymbionts genetic diversity carried by cattle ticks in Algeria.

    abstract：:Coxiella-like bacteria are a large group of yet-to-isolate and characterize bacteria phylogenetically close to the agent of Q fever, Coxiella burnetii, and often associated with ixodid ticks worldwide. This study was designed to assess the presence of Coxiella-like endosymbionts (CLE) in ticks and to describe their ge...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2020.101493

    authors： Rahal M,Medkour H,Diarra AZ,Bitam I,Parola P,Mediannikov O

    更新日期：2020-09-01 00:00:00

  • Anaplasma marginale outer membrane protein vaccine candidates are conserved in North American and South African strains.

    abstract：:Bovine anaplasmosis is a globally economically important tick-borne disease caused by the obligate intraerythrocytic rickettsia, Anaplasma marginale. A live Anaplasma centrale blood-based vaccine is available, but it does not protect against all A. marginale field strains and may also transmit other blood-borne pathog...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2020.101444

    authors： Hove P,Brayton KA,Liebenberg J,Pretorius A,Oosthuizen MC,Noh SM,Collins NE

    更新日期：2020-07-01 00:00:00

  • Rickettsia amblyommatis isolated from Amblyomma mixtum (Acari: Ixodida) from two sites in Panama.

    abstract：:Rickettsia amblyommatis is widely distributed in the Americas, and has been reported to infect different species of ticks within its distribution. In Panama, R. amblyommatis is the most common Rickettsia and its presence was molecularly detected in nine species of ticks and one flea species. This work described the is...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2020.101597

    authors： Bermúdez C S,Zaldívar Y,Domínguez A L,Hernández M,de Antinori MEB,Krawczak FS

    更新日期：2021-01-01 00:00:00

  • Associations between Ixodes scapularis ticks and small mammal hosts in a newly endemic zone in southeastern Canada: implications for Borrelia burgdorferi transmission.

    abstract：:Immature Ixodes scapularis infestation and Borrelia burgdorferi infection of wild small mammals were studied from June to October in 2007 and from May to October in 2008 at 71 study sites in a zone where I. scapularis populations and environmental Lyme disease risk are emerging in southwestern Quebec. Seasonal host-se...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2011.03.005

    authors： Bouchard C,Beauchamp G,Nguon S,Trudel L,Milord F,Lindsay LR,Bélanger D,Ogden NH

    更新日期：2011-12-01 00:00:00

  • Infection of Ixodes spp. tick cells with different Anaplasma phagocytophilum isolates induces the inhibition of apoptotic cell death.

    abstract：:Anaplasma phagocytophilum is an intracellular rickettsial pathogen transmitted by Ixodes spp. ticks, which causes granulocytic anaplasmosis in humans, horses and dogs and tick-borne fever (TBF) in ruminants. In the United States, human granulocytic anaplasmosis (HGA) is highly prevalent while TBF has not been reported...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2015.07.001

    authors： Alberdi P,Ayllón N,Cabezas-Cruz A,Bell-Sakyi L,Zweygarth E,Stuen S,de la Fuente J

    更新日期：2015-09-01 00:00:00

  • Babesia bovis infection in cattle in the southwestern Brazilian Amazon.

    abstract：:The present study provides the first epidemiological data on infection with Babesia bovis in cattle raised in the southwestern Brazilian Amazon. Blood clot samples were filtered through nylon cloth before being submitted to DNA extraction. PCR and nested-PCR were applied to assess the frequency of infection with B. bo...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2012.08.001

    authors： Brito LG,Rocha RB,Barbieri Fda S,Ribeiro ES,Vendrami FB,Souza GC,Giglioti R,Regitano LC,Falcoski TO,Tizioto PC,Oliveira MC

    更新日期：2013-02-01 00:00:00

  • Rhipicephalus (Boophilus) microplus tick in vitro feeding methods for functional (dsRNA) and vaccine candidate (antibody) screening.

    abstract：:Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) ticks cause economic losses for cattle industries throughout tropical and subtropical regions of the world estimated at $US2.5 billion annually. Lack of access to efficacious long-lasting vaccination regimes and increases in tick acaricide resistance have led to th...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2014.03.005

    authors： Lew-Tabor AE,Bruyeres AG,Zhang B,Rodriguez Valle M

    更新日期：2014-09-01 00:00:00

  • Preventing tick-bites among children in Indiana, USA: An analysis of factors associated with parental protective behaviors.

    abstract：:Despite evidence to the effect that there is low parental adoption of tick-bite personal protective behavior (PPB) for their children - a population at high risk for tick exposure, very limited information is available on factors associated with parental adoption of PPB. The objective of this study was to identify the...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2020.101647

    authors： Omodior O,Anderson KR,Clark W,Eze P,Donohoe H

    更新日期：2021-03-01 00:00:00

  • Identical 18S rRNA haplotypes of Hepatozoon canis in dogs and foxes in Brandenburg, Germany.

    abstract：:Hepatozoon canis is a blood parasite of the suborder Adeleorina infecting wild and domestic canids. Transmission occurs by oral uptake of Rhipicephalus sanguineus sensu lato vector ticks infected with H. canis, but vertical transmission is also assumed to be possible. In German foxes, a high prevalence of H. canis has...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2020.101520

    authors： Helm CS,Samson-Himmelstjerna GV,Liesner JM,Kohn B,Müller E,Schaper R,Pachnicke S,Schulze C,Krücken J

    更新日期：2020-11-01 00:00:00

  • The role of Ixodes scapularis, Borrelia burgdorferi and wildlife hosts in Lyme disease prevalence: A quantitative review.

    abstract：:Due to the ongoing expansion of Ixodes scapularis (blacklegged tick) throughout the northeastern and midwestern United States, there is need to identify the role wildlife hosts play in the establishment and maintenance of tick populations. To quantify and synthesize the patterns of I. scapularis and Borrelia burgdorfe...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.ttbdis.2018.04.006

    authors： Halsey SJ,Allan BF,Miller JR

    更新日期：2018-07-01 00:00:00

  • Huge diversity of phleboviruses in ticks from Strandja Nature Park, Bulgaria.

    abstract：:The discovery of the first tick-borne phleboviruses associated with severe disease in humans stimulated studies searching for further previously unknown tick-associated viruses. Novel phleboviruses have subsequently been identified in ticks from the USA, Japan and China and recently also from Europe. Here, we investig...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2019.03.001

    authors： Ohlendorf V,Marklewitz M,Kopp A,Yordanov S,Drosten C,Junglen S

    更新日期：2019-04-01 00:00:00

  • A life stage-targeted acaricide application approach for the control of Haemaphysalis longicornis.

    abstract：:Haemaphysalis longicornis Neumann is an invasive tick species that has recently been detected in the eastern United States. We designed field studies to monitor the population dynamics of H. longicornis over a year (2019) in New Jersey, and to assess the efficacy of a pyrethroid acaricide (lambda-cyhalothrin) in contr...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2020.101581

    authors： Bickerton M,McSorley K,Toledo A

    更新日期：2021-01-01 00:00:00

  • A matrix metalloproteinase 9 (MMP9) gene single nucleotide polymorphism is associated with predisposition to tick-borne encephalitis virus-induced severe central nervous system disease.

    abstract：:The progression of infectious diseases depends on causative agents, the environment and the host's genetic susceptibility. To date, human genetic susceptibility to tick-borne encephalitis (TBE) virus-induced disease has not been sufficiently studied. We have combined whole-exome sequencing with a candidate gene approa...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2018.02.010

    authors： Barkhash AV,Yurchenko AA,Yudin NS,Ignatieva EV,Kozlova IV,Borishchuk IA,Pozdnyakova LL,Voevoda MI,Romaschenko AG

    更新日期：2018-05-01 00:00:00

  • Ticks and Rickettsia on anteaters from Southeast and Central-West Brazil.

    abstract：:The giant anteater (Myrmecophaga tridactyla) and the collared anteater (Tamandua tetradactyla) are widespread in Brazil and found in all Brazilian biomes. These hosts frequently use domestic animal environments such as pastures, where tick and related microorganism interchange may occur between hosts. Reports of tick ...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2019.01.008

    authors： Szabó MPJ,Pascoal JO,Martins MM,Ramos VDN,Osava CF,Santos ALQ,Yokosawa J,Rezende LM,Tolesano-Pascoli GV,Torga K,de Castro MB,Suzin A,Barbieri ARM,Werther K,Silva JMM,Labruna MB

    更新日期：2019-04-01 00:00:00

  • Coxiella symbiont in the tick Ornithodoros rostratus (Acari: Argasidae).

    abstract：:In the present study, the presence of tick-associated bacteria and protozoa in Ornithodoros rostratus ticks (adults, nymphs, and eggs) from the Pantanal region of Brazil were determined by molecular detection. In these ticks, DNA from protozoa in the genera Babesia and Hepatozoon, and bacteria from the genera Ricketts...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2012.02.003

    authors： Almeida AP,Marcili A,Leite RC,Nieri-Bastos FA,Domingues LN,Martins JR,Labruna MB

    更新日期：2012-09-01 00:00:00

  • Tick-borne pathogens in the European polecat, Mustela putorius and in attached Ixodes hexagonus ticks from Germany.

    abstract：:The European polecat, Mustela putorius, occurs almost throughout Europe. However, there is a lack of data on the ectoparasite fauna and the potential role in the circulation of tick-borne pathogens (TBP) of this mustelid species. The aim of this study was to investigate whether M. putorius contributes to the maintenan...

    journal_title：Ticks and tick-borne diseases

    pub_type： 杂志文章

    doi：10.1016/j.ttbdis.2019.02.004

    authors： Król N,Obiegala A,Kretschmar FM,Hamel D,Pfeffer M

    更新日期：2019-04-01 00:00:00