Abstract：
:Suspicion of Brazilian spotted fever (BSF) should occur in endemic regions upon surveillance of the acute febrile icteric hemorrhagic syndrome (AFIHS). However, limitations associated with currently available laboratory tests pose a challenge to early diagnosis, especially in fatal cases. Two real-time PCR (qPCR) protocols were evaluated to diagnose BSF in 110 fatal AFIHS cases, collected in BSF-endemic regions in 2009-2010. Of these, 24 were positive and 86 negative by indirect immunofluorescence (IFA) assay (cut-off IgG and/or IgM ≥ 128). DNA from these samples was used in the qPCR protocols: one to detect Rickettsia spp. (citrate synthase gene) and another to determine spotted fever group (SFG) Rickettsia species (OmpA gene). Of the 24 IFA-positive samples, 5 (21%) were positive for OmpA and 9 (38%) for citrate synthase. In the IFA-negative group (n=86), OmpA and citrate synthase were positive in 23 (27%) and 27 (31%), respectively. These results showed that the 2 qPCR protocols were about twice as sensitive as the IFA test alone (93% concordance). In conclusion, qPCR is a sensitive method for the diagnosis of fatal BSF cases and should be considered for routine surveillance of AFIHS in places like Brazil, where spotted fever-related lethality is high and other endemic diseases like dengue and leptospirosis can mislead diagnosis.
journal_name
Ticks Tick Borne Disjournal_title
Ticks and tick-borne diseasesauthors
dos Santos FC,do Nascimento EM,Katz G,Angerami RN,Colombo S,de Souza ER,Labruna MB,da Silva MVdoi
10.1016/j.ttbdis.2012.10.027subject
Has Abstractpub_date
2012-12-01 00:00:00pages
312-4issue
5-6eissn
1877-959Xissn
1877-9603pii
S1877-959X(12)00112-4journal_volume
3pub_type
杂志文章abstract：:Due to the availability of adequate habitats in urban environments, e.g. city parks and recreational green areas, ticks from such settings may also carry pathogens of veterinary and public health concern. Thus, tick-borne infections may readily be identified in companion animals residing in urbanised areas. To investi...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2012.08.005
更新日期：2013-02-01 00:00:00
abstract：:Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum are two tick-borne pathogens of medical and/or veterinary importance which are distributed worldwide. Erinaceus roumanicus, the Northern white-breasted hedgehog, is a common synanthropic species that is known to carry not only the hedgehog tick, Ixodes hexa...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2012.11.010
更新日期：2013-04-01 00:00:00
abstract：:The current need of identification of a new acaricidal agent which is acceptable to public as environmentally safe is a daring task. Use of herbal acaricides is such an alternative. Most of the herbal extracts or fractions are dissolved in polar or non-polar solvents or detergents before tested for acaricidal activity...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2011.04.001
更新日期：2011-09-01 00:00:00
abstract：:The lone star tick, Amblyomma americanum (L.) (Acari: Ixodidae), is established in southeastern Nebraska yet the prevalence of tick-associated microorganisms is not known. An initial PCR-based analysis for Ehrlichia chaffeensis, Ehrlichia ewingii, and Borrelia infection in host-seeking adult ticks collected in southea...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2015.10.008
更新日期：2016-02-01 00:00:00
abstract：:For more than 50 years DEET (N,N-Diethyl-m-toluamide) has been considered the gold standard of repellents. It is applied to the skin or clothing to deter mosquitoes and other blood-sucking invertebrate pests from approaching and/or settling, and ultimately it provides temporary protection from bites. Despite rampant g...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.12.006
更新日期：2019-04-01 00:00:00
abstract：:Kyasanur Forest disease virus (KFDV) and Alkhurma hemorrhagic fever virus (AHFV) are enveloped, positive-stranded RNA viruses of clinical importance with complex enzootic life cycles involving hematophagous ticks which feed on small and large mammals. Humans and monkeys are dead-end hosts for these viruses. Recent tre...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2020.101381
更新日期：2020-05-01 00:00:00
abstract：:Equine piroplasmosis (EP) is an important tick-borne disease of equids, caused by Theileria equi and Babesia caballi. It is endemic in most parts of the world, including Israel, and has clinical and economic consequences. This study was set to evaluate the presence of EP parasites in domestic donkeys and in wild equid...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.101286
更新日期：2020-01-01 00:00:00
abstract：:Cervid babesiosis, caused by the protozoan hemoparasite Babesia odocoilei and transmitted by the blacklegged tick Ixodes scapularis, is an emerging disease of Canadian cervids. This pathogen has not yet been described in humans. Data are lacking on the role of migratory birds in the adventitious spread of Ba. odocoile...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.02.016
更新日期：2019-04-01 00:00:00
abstract：:All of the parasitic stages of Ixodes inopinatus n. sp. are described from specimens collected by flagging and on lizards and foxes. The new species replaces I. ricinus in dry areas of the Mediterranean region in Spain, Portugal, Morocco, Algeria and Tunisia. It has also been collected in areas of western Germany in s...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2014.05.003
更新日期：2014-10-01 00:00:00
abstract：:Rickettsia parkei is the etiological agent of spotted fever in Uruguay, where is transmitted to humans by the tick Amblyomma triste. In the present study, ticks were collected from capybaras (Hydrochoerus hydrochaeris) and domestic dogs during 2011-2012 in different parts of Uruguay. Three out of 11 (27.3%) Amblyomma ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2014.04.021
更新日期：2014-10-01 00:00:00
abstract：:Surveillance indicates that tick-borne diseases are a common problem in the United States. Nevertheless, little is known regarding the experience or management practices of healthcare providers who treat these conditions. The purpose of the present study was to characterize the frequency of tick-borne diseases in clin...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2014.01.008
更新日期：2014-06-01 00:00:00
abstract：:The progression of infectious diseases depends on causative agents, the environment and the host's genetic susceptibility. To date, human genetic susceptibility to tick-borne encephalitis (TBE) virus-induced disease has not been sufficiently studied. We have combined whole-exome sequencing with a candidate gene approa...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.02.010
更新日期：2018-05-01 00:00:00
abstract：:The hard tick Ixodes ricinus is the principal vector of Lyme borreliosis (LB) group spirochaetes in Europe, but it also transmits a large number of other microbial pathogens that are of importance to animal and human health. Here, we characterise geographically distinct populations of this important ectoparasite based...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2013.10.001
更新日期：2014-03-01 00:00:00
abstract：:Rodents are important reservoir hosts of tick-borne pathogens. Anaplasma phagocytophilum is the causative agent of granulocytic anaplasmosis of both medical and veterinary importance. In Europe, this pathogen is primarily transmitted by the Ixodes ricinus tick among a wide range of vertebrate hosts. However, to what d...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2014.07.012
更新日期：2014-10-01 00:00:00
abstract：:The raccoon (Procyon lotor) and the raccoon dog (Nyctereutes procyonoides) belong to a group of the invasive species. The introduced species as potential reservoirs for vector-borne pathogens have been the subject of recent research, though there are still no data with reference to the European population of the racco...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.03.018
更新日期：2018-05-01 00:00:00
abstract：:Anaplasma species are obligate intracellular rickettsial pathogens that affect the health of humans and animals. In this study, we investigated the presence and frequency of Anaplasma species by 16S rRNA PCR-RLB, RFLP, and DNA sequencing in 200 apparently healthy cattle. Anaplasma spp. overall infection rate was 38.5 ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2020.101604
更新日期：2021-01-01 00:00:00
abstract：:There are 4 major human-biting tick species in the northeastern United States, which include: Amblyomma americanum, Amblyomma maculatum, Dermacentor variabilis, and Ixodes scapularis. The black bear is a large mammal that has been shown to be parasitized by all the aforementioned ticks. We investigated the bacterial i...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2012.12.002
更新日期：2013-04-01 00:00:00
abstract：:Anaplasma phagocytophilum is an obligate intracellular and tick-transmitted bacterium, which causes granulocytic anaplasmosis in animals and humans. Although infection with A. phagocytophilum in domestic animals and vector ticks is documented, there is sparse information on the occurrence of A. phagocytophilum in wild...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2013.11.001
更新日期：2014-04-01 00:00:00
abstract：:Sardinia is a hotspot for studying tick-borne diseases in the Mediterranean region, where cases of notifiable tick-borne diseases are increasing. The aim of this study was to determine the presence of tick-borne bacteria of medical and veterinary importance in ixodid ticks collected from domestic and wild animals, hum...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.02.008
更新日期：2018-03-01 00:00:00
abstract：:Workers at veterinary clinics are exposed to zoonotic pathogens, which, though infrequently, can lead to serious consequences. The aim of the present study was to estimate the seroprevalence for rickettsial pathogens in veterinary clinic personnel in Ciudad Juárez, Chihuahua, Mexico. Voluntary participants included 10...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.10.012
更新日期：2019-02-01 00:00:00
abstract：:Rickettsial pathogens cause diseases that vary in severity and clinical presentation. Rickettsia species transmitted by ticks are mostly classified within the spotted fever group of rickettsiae (SFGR) and are often associated with febrile diseases. Preliminary studies have detected three human-pathogenic SFGR from tic...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2020.101470
更新日期：2020-09-01 00:00:00
abstract：:Anaplasma phagocytophilum is an emerging zoonotic tick-borne pathogen affecting a wide range of mammals. Rodents are suspected to be natural reservoirs for this bacterium, but their role in the epidemiologic cycles affecting domestic animals and wild ungulates has not been demonstrated. This study aimed to improve our...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2016.05.005
更新日期：2016-07-01 00:00:00
abstract：:Ticks and tick-borne disease (TBD) are major challenges to cattle production in the tropics and subtropics. Economic losses associated with ticks amount to billions of dollars annually. Although efforts to eradicate ticks and TBD using chemical control strategies have been implemented in many developing countries for ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章,评审
doi：10.1016/j.ttbdis.2014.04.006
更新日期：2014-09-01 00:00:00
abstract：:Anaplasma phagocytophilum is an intracellular rickettsial pathogen transmitted by Ixodes spp. ticks, which causes granulocytic anaplasmosis in humans, horses and dogs and tick-borne fever (TBF) in ruminants. In the United States, human granulocytic anaplasmosis (HGA) is highly prevalent while TBF has not been reported...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2015.07.001
更新日期：2015-09-01 00:00:00
abstract：:Previously, we reported that Coxiella-like, Rickettsia-like and Arsenophonus-like symbionts could simultaneously coexist in Dermacentor silvarum. In this study, we examined their burdens and population dynamics in a single host during the host life cycle using quantitative PCR. Our results showed that multiple symbion...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2015.10.002
更新日期：2016-02-01 00:00:00
abstract：:A marked increase in tick-borne encephalitis (TBE) incidence has been observed in Europe during the last 2 decades. Hypothetical causes include global climatic fluctuations, human-induced environmental changes, and socio-economic changes. These factors are thought to be disproportionately relevant in different geograp...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2010.05.003
更新日期：2010-09-01 00:00:00
abstract：:This study is the first report about the prevalence of tick-borne pathogens, as well as their (co-)infection rates, in the museum-archived I. ricinus female ticks collected in Moldova in 1960. A total of 16.7% (21/126) ticks was mono-infected. Borrelia burgdorferi sensu stricto was revealed as the most abundant specie...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2012.12.004
更新日期：2013-06-01 00:00:00
abstract：:Tick-borne encephalitis (TBE) is the most important viral infection transmitted by ticks in Central Europe. In Germany, where TBE was classified as a notifiable disease in 2001, a highly variable number of clinically apparent human cases was reported in the last few years, ranging from the lowest number of 238 in 2007...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2009.11.002
更新日期：2010-03-01 00:00:00
abstract：:Amblyomma mixtum Koch, 1844 parasitizes livestock, humans, and wildlife in Mexico. However, information on population genetics for this tick species in the country is missing. Tick samples were collected from livestock in ten regions across the state of Veracruz (22°28'N, 17°09'S, 93°36'E, 98°39'W) to analyze the gene...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.09.004
更新日期：2019-01-01 00:00:00
abstract：:Despite evidence to the effect that there is low parental adoption of tick-bite personal protective behavior (PPB) for their children - a population at high risk for tick exposure, very limited information is available on factors associated with parental adoption of PPB. The objective of this study was to identify the...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2020.101647
更新日期：2021-03-01 00:00:00
