Abstract：
:Tick-borne encephalitis virus (TBEV) is a zoonotic agent causing severe encephalitis in humans. A wide range of animal species could be infected with TBEV in endemic areas. A serological survey of wild animals is effective in identifying TBEV-endemic areas. Safe, simple, and reliable TBEV serodiagnostic tools are needed to test animals. In this study, ELISA was developed to detect anti-TBEV specific antibodies in multi-species of animals, using recombinant subviral particles (SPs) with an affinity tag and protein A/G. A Strep-tag was fused at the N terminus of the E protein of the plasmid coding TBEV prME. The E proteins with Strep-tag were secreted as SPs, of which Strep-tag was exposed on the surface. The tagged E proteins were associated with prM. The SPs with Strep-tag were applied as the antigen of ELISA, and TBEV-specific antibodies were detected by the protein A/G. Compared to neutralization test results, the ELISA showed 96.8% sensitivity and 97.7% specificity in rodents and 95.1% sensitivity and 96.0% specificity in humans, without cross-reactivity with antibodies to Japanese encephalitis virus. These results indicate that our ELISA would be useful to detect TBE-specific antibodies in a wide range of animal species.
journal_name
Ticks Tick Borne Disjournal_title
Ticks and tick-borne diseasesauthors
Inagaki E,Sakai M,Hirano M,Muto M,Kobayashi S,Kariwa H,Yoshii Kdoi
10.1016/j.ttbdis.2016.03.002subject
Has Abstractpub_date
2016-07-01 00:00:00pages
723-729issue
5eissn
1877-959Xissn
1877-9603pii
S1877-959X(16)30039-5journal_volume
7pub_type
杂志文章abstract：:The entomopathogenic fungus Metarhizium anisopliae is used as a biological pest control agent against various arthropod species, including ticks. However, the efficacy depends on tick species, tick stage and fungus strain. We studied the effect of M. anisopliae on engorged larvae and nymphs of Ixodes ricinus, the most...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2016.03.010
更新日期：2016-07-01 00:00:00
abstract：:Rodents are important reservoir hosts of tick-borne pathogens. Anaplasma phagocytophilum is the causative agent of granulocytic anaplasmosis of both medical and veterinary importance. In Europe, this pathogen is primarily transmitted by the Ixodes ricinus tick among a wide range of vertebrate hosts. However, to what d...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2014.07.012
更新日期：2014-10-01 00:00:00
abstract：:Human rickettsioses caused by Rickettsia parkeri strain Atlantic rainforest in Brazil motivated the analysis of Amblyomma ovale ticks in Misiones province, a similar ecological region in northeastern Argentina. During 2010-2017, 393 A. ovale ticks were collected from domestic and wild animals and from vegetation, and ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.05.007
更新日期：2018-07-01 00:00:00
abstract：:The raccoon (Procyon lotor) and the raccoon dog (Nyctereutes procyonoides) belong to a group of the invasive species. The introduced species as potential reservoirs for vector-borne pathogens have been the subject of recent research, though there are still no data with reference to the European population of the racco...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.03.018
更新日期：2018-05-01 00:00:00
abstract：:Sardinia is a hotspot for studying tick-borne diseases in the Mediterranean region, where cases of notifiable tick-borne diseases are increasing. The aim of this study was to determine the presence of tick-borne bacteria of medical and veterinary importance in ixodid ticks collected from domestic and wild animals, hum...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.02.008
更新日期：2018-03-01 00:00:00
abstract：:The aim of this study was to describe the dynamics of the long-legged bat tick Ixodes vespertilionis infestation on the lesser horseshoe bat Rhinolophus hipposideros in 2 nursery colonies roosting in attics. Out of a total of 810 lesser horseshoe bats examined, 217 (26.8%) were found to be infested with a total of 464...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2013.08.006
更新日期：2014-02-01 00:00:00
abstract：:The European polecat, Mustela putorius, occurs almost throughout Europe. However, there is a lack of data on the ectoparasite fauna and the potential role in the circulation of tick-borne pathogens (TBP) of this mustelid species. The aim of this study was to investigate whether M. putorius contributes to the maintenan...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.02.004
更新日期：2019-04-01 00:00:00
abstract：:Acaricide resistance is one of the greatest threats towards the successful control of vector ticks worldwide. Communal farmers of Zimbabwe use amitraz as the most common acaricide with occasional usage of pyrethroids and organophosphates. As a strategy towards developing an effective acaricide resistance management sy...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.10.017
更新日期：2018-01-01 00:00:00
abstract：:Amblyomma rotundatum is an ixodid tick strictly associated with cold-blooded animals, among them the toad Rhinella jimi. This work aimed to evaluate the seasonal dynamics of larvae, nymphs, and adults of A. rotundatum parasitizing R. jimi in an area within the semi-arid Caatinga Biome of northeastern Brazil. Monthly s...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.06.005
更新日期：2018-07-01 00:00:00
abstract：:Rodents are hosts of numerous pathogenic agents of public health importance globally. Their ability to harbor these pathogens without showing overt clinical signs of disease has epidemiologic consequences. In some rural settings in Nigeria, humans and rodents do not only share feeds and abode, but the latter may end u...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.10.004
更新日期：2018-01-01 00:00:00
abstract：:Rickettsia rickettsii is the agent of Brazilian spotted fever (BSF), the most lethal tick-borne disease in the western hemisphere. Amblyomma sculptum is the main vector of R. rickettsii in southeastern Brazil. Capybaras act as primary hosts for this tick species, and as amplifying hosts for R. rickettsii, creating new...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2020.101463
更新日期：2020-09-01 00:00:00
abstract：:The present study provides the first epidemiological data on infection with Babesia bovis in cattle raised in the southwestern Brazilian Amazon. Blood clot samples were filtered through nylon cloth before being submitted to DNA extraction. PCR and nested-PCR were applied to assess the frequency of infection with B. bo...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2012.08.001
更新日期：2013-02-01 00:00:00
abstract：:Canine vector borne diseases include a variety of illnesses affecting domestic dogs worldwide. Clinical abnormalities are often nonspecific during rickettsial infections, and coinfections caused by other tick-transmitted agents may be common. The aim of this study was to validate a differential serological assay for t...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2012.10.024
更新日期：2012-12-01 00:00:00
abstract：:Immature Ixodes scapularis infestation and Borrelia burgdorferi infection of wild small mammals were studied from June to October in 2007 and from May to October in 2008 at 71 study sites in a zone where I. scapularis populations and environmental Lyme disease risk are emerging in southwestern Quebec. Seasonal host-se...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2011.03.005
更新日期：2011-12-01 00:00:00
abstract：:Due to the availability of adequate habitats in urban environments, e.g. city parks and recreational green areas, ticks from such settings may also carry pathogens of veterinary and public health concern. Thus, tick-borne infections may readily be identified in companion animals residing in urbanised areas. To investi...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2012.08.005
更新日期：2013-02-01 00:00:00
abstract：:The current need of identification of a new acaricidal agent which is acceptable to public as environmentally safe is a daring task. Use of herbal acaricides is such an alternative. Most of the herbal extracts or fractions are dissolved in polar or non-polar solvents or detergents before tested for acaricidal activity...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2011.04.001
更新日期：2011-09-01 00:00:00
abstract：:The giant anteater (Myrmecophaga tridactyla) and the collared anteater (Tamandua tetradactyla) are widespread in Brazil and found in all Brazilian biomes. These hosts frequently use domestic animal environments such as pastures, where tick and related microorganism interchange may occur between hosts. Reports of tick ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.01.008
更新日期：2019-04-01 00:00:00
abstract：:Certain countries including the United States remain non-endemic for particular infectious diseases such as equine piroplasmosis through import restrictions and surveillance. Endemic regions often employ premunition as the primary method to control disease, however in non-endemic countries, chemosterilization combined...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.08.007
更新日期：2018-02-01 00:00:00
abstract：:Theileria parva is a tick-transmitted protozoan parasite that causes a disease called East Coast fever (ECF) in cattle. This important tick borne-disease (TBD) causes significant economic losses in cattle in many sub-Saharan countries, including Tanzania. Cattle immunization using Muguga cocktail has been recommended ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.05.007
更新日期：2019-08-01 00:00:00
abstract：:Thymol is a monoterpene present in plants of the families Lamiaceae, Verbenaceae and Apiaceae. Despite its proven acaricidal activity, little is known about the mechanism of action of thymol in ticks. Thus, the aim of this study was to perform a morpho-histochemical analysis of the synganglion and salivary glands of p...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.11.003
更新日期：2019-02-01 00:00:00
abstract：:Lyme borreliosis (LB) is the most important human tick-borne disease, but Borrelia genospecies cause different clinical manifestations. Ticks of the genus Ixodes removed from humans between 2006 and 2012 were analysed for Borrelia burgdorferi sensu lato (sl) infections. The majority of ticks originated from the Greate...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.05.003
更新日期：2017-08-01 00:00:00
abstract：:The kangaroo soft tick, Ornithodoros gurneyi Warburton, 1926, is a poorly studied argasid of the more arid regions of Australia. Anecdotal accounts have stated that this tick species will bite humans, and yet bites have not been described. Herein we report on an instance of parasitism by a nymph of O. gurneyi on a hum...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2020.101632
更新日期：2021-03-01 00:00:00
abstract：:Complement is a key first line innate host defense system in the blood of vertebrates. Upon activation, this powerful defense mechanism can elicit inflammatory responses, lyse non-self-cells, or mark them for opsonophagocytic removal. Blood-feeding arthropods thus require the ability to block host complement activatio...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.101354
更新日期：2020-03-01 00:00:00
abstract：:Ixodes scapularis is responsible for the transmission of a variety of pathogens in North America, including Borrelia burgdorferi sensu stricto, Anaplasma phagocytophilum and Babesia microti. Songbirds have previously been described as agents of tick dispersal, and a combination of empirical data and modeling efforts h...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.05.012
更新日期：2019-08-01 00:00:00
abstract：:Tick-borne diseases (TBD) occur in many temperate countries and are economically important in most tropical and subtropical areas, affecting dairy and beef cattle, as well as small ruminants. Four major tick-borne diseases have been detected in eastern and southern Africa: East Coast fever (ECF) caused by Theileria pa...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.101283
更新日期：2020-01-01 00:00:00
abstract：:Acaricide resistance is one of the greatest threats to sustainable and effective control of vector ticks worldwide. The amitraz resistance status in cattle tick, Rhipicephalus microplus populations collected from 18 districts of Punjab in north-western India were characterized using bioassay and molecular assays. The ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2020.101578
更新日期：2021-01-01 00:00:00
abstract：:Due to the ongoing expansion of Ixodes scapularis (blacklegged tick) throughout the northeastern and midwestern United States, there is need to identify the role wildlife hosts play in the establishment and maintenance of tick populations. To quantify and synthesize the patterns of I. scapularis and Borrelia burgdorfe...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.ttbdis.2018.04.006
更新日期：2018-07-01 00:00:00
abstract：:Livestock and their ectoparasites are involved in the epidemiology of several zoonotic diseases. Studies regarding the molecular detection of infectious agents in ticks from Northwestern Algeria are scarce. Thus, the presence of spotted fever group Rickettsia spp., Anaplasmataceae microorganisms and Coxiella burnetii ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.04.018
更新日期：2019-06-01 00:00:00
abstract：:The seasonal risk to humans of picking up Ixodes ricinus ticks in different habitats at 3 recreational sites in the UK was assessed. A comprehensive range of vegetation types was sampled at 3-weekly intervals for 2 years, using standard blanket-dragging complemented by woollen leggings and square 'heel flags'. Ticks w...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2011.03.002
更新日期：2011-06-01 00:00:00
abstract：:A cross-sectional study combining different serological and molecular techniques for the detection of Ehrlichia species in dogs and their ticks was carried out with data from all regions of Costa Rica. A seroprevalence of 32.1% (131/408), and infection with E. canis of 3.2% (13/407) was found, whereas 6.9% (9/130) of ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2016.07.006
更新日期：2016-10-01 00:00:00
