Bacterial Contamination of Blood DNA Samples is Associated with Donor's Health Condition.

Abstract:

:Bacterial contamination often occurs in human blood DNA samples, possibly due to bacteremia or an inappropriate procedure during sample preparation. This study aimed at analyzing the clinical significance of bacterial DNA contamination in human blood DNA samples and to assess its influence on experimental data. DNA samples (N = 1359) were randomly selected from population-based cohort samples to determine bacterial DNA contamination by polymerase chain reaction and direct DNA sequencing. Bacterial DNA contaminated samples (N = 150) were then assessed for experimental quality of single nucleotide polymorphism (SNP) chip data, compared with uncontaminated DNA samples (N = 1209). DNA sequencing data showed that a major source of bacterial contaminants was derived from Alcaligenes species. The occurrence of bacterial DNA contaminations was significantly associated with some clinical variables including a postprandial glucose level at 60 min, % body fat, and waist-to-hip ratio. It was also found that there was no difference of SNP call rates between bacterial DNA contaminated samples and uncontaminated DNA samples. This study showed that bacterial DNA contamination in human blood samples was related to donor's health condition, suggesting that the occurrence of bacterial DNA contamination may provide useful health information of blood donors and a potential tool for human disease genomics.

journal_name

Biopreserv Biobank

authors

Lee JE,Hong EJ,Shim SM,Kim JW,Bae GR,Cho YS,Jeon JP,Han BG

doi

10.1089/bio.2010.0012

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

127-31

issue

3

eissn

1947-5535

issn

1947-5543

journal_volume

8

pub_type

杂志文章
  • Advancing Professionalization of Biobank Business Operations: Performance and Utilization.

    abstract::Biobanks are now in the spotlight as key enablers supporting preclinical, clinical, and environmental research. Awareness of their value has increased along with the need for these infrastructures to be sustained through business-focused practices. Following our 2017 pilot survey on biobank business planning, we initi...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2019.0005

    authors: Henderson MK,Goldring K,Simeon-Dubach D

    更新日期:2019-06-01 00:00:00

  • Impact of a Permission to Contact (PTC) platform on biobank enrollment and efficiency.

    abstract::The consent process involves three steps; referral for contact, preliminary interview, and informed consent discussion. We propose that the efficiency and frequency of the consent process for individual biobank related projects increases when the referral for contact is conducted by an independent "Permission to Conta...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2013.0004

    authors: LeBlanc J,Dee S,Braun L,Daudt H,Cheah S,Watson PH

    更新日期:2013-06-01 00:00:00

  • Challenges for the Sustainability of University-Run Biobanks.

    abstract::Most university biobanks begin like other university research projects, that is, with an idea conceived by an individual researcher in pursuit of his/her own research interests, publications, funding, and career. Some biobanks, however, come to have scientific value that goes beyond the projects that were initially re...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2018.0054

    authors: Kongsholm NCH,Christensen ST,Hermann JR,Larsen LA,Minssen T,Pedersen LB,Rajam N,Tommerup N,Tupasela A,Schovsbo J

    更新日期:2018-08-01 00:00:00

  • Following nature's lead: generating compounds for stabilizing biomolecules.

    abstract::We describe here a unique approach to identifying small molecules that are useful in stabilizing biomolecules in the dry and liquid states. Using biostability screens aided by in silico docking experiments and synthetic chemistry, libraries of biostability molecules are analyzed for their ability to protect important ...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2012.0022

    authors: Clement O,Whitney S,Muller-Cohn J,Muller R

    更新日期:2012-08-01 00:00:00

  • Phase Change Behavior of NaCl-H2O Binary Solution Under the Control of AC Electric Field.

    abstract::Cryopreservation, which refers to preservation of cells or tissues at subzero temperatures, inevitably involves the problem of cryoinjury caused by ice crystals. The application of an external electric field during the freezing process has been shown to be a promising approach to produce miniature ice grains and decre...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2020.0045

    authors: Ma Y,Fan X,Cai J,Tao J

    更新日期:2020-10-16 00:00:00

  • The influence of tissue procurement procedures on RNA integrity, gene expression, and morphology in porcine and human liver tissue.

    abstract::The advent of molecular characterization of tissues has brought an increasing emphasis on the quality of biospecimens, starting with the tissue procurement process. RNA levels are particularly affected by factors in the collection process, but the influence of different pre-analytical factors is not well understood. H...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2014.0076

    authors: Kap M,Sieuwerts AM,Kubista M,Oomen M,Arshad S,Riegman P

    更新日期:2015-06-01 00:00:00

  • The human face of biobank networks for translational research.

    abstract::The biobanking literature frequently addresses donor and societal issues surrounding biobanking, but the biobanker's perspective is rarely highlighted. While not comprehensive, this article offers an overview of the human aspects of biobanking from the viewpoint of biobank personnel-from biobank formation, through the...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2011.0018

    authors: Meir K,Gaffney EF,Simeon-Dubach D,Ravid R,Watson PH,Schacter B,Morente And The Marble Arch International Working Group On Biobanking MM

    更新日期:2011-09-01 00:00:00

  • A Simple Variable Number of Tandem Repeat-Based Genotyping Strategy for the Detection of Handling Errors and Validation of Sample Identity in Biobanks.

    abstract::Biobanking biological samples involve multiple handling, processing, and labeling steps. Each step may be a source of error, which if unnoticed or uncorrected may have consequences for research. We aimed to develop a simple and inexpensive genotyping method that would be valuable to detect such errors and confirm samp...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2015.0113

    authors: Pellerin C,McKercher G,Aprikian AG,Saad F,Lacombe L,Carmel M,Chevalier S

    更新日期:2016-10-01 00:00:00

  • Standardized Serum-Free Cryomedia Maintain Peripheral Blood Mononuclear Cell Viability, Recovery, and Antigen-Specific T-Cell Response Compared to Fetal Calf Serum-Based Medium.

    abstract::The ability to analyze cryopreserved peripheral blood mononuclear cells (PBMCs) from biobanks for antigen-specific T-cell immunity is necessary to evaluate responses to immune-based therapies. Comprehensive studies have demonstrated that the quality of frozen PBMCs is critical and the maintenance of cell viability and...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2010.0033

    authors: Germann A,Schulz JC,Kemp-Kamke B,Zimmermann H,von Briesen H

    更新日期:2011-09-01 00:00:00

  • Biobanked Amphibian Samples Confirmed To Species Level Using 16S rRNA DNA Barcodes.

    abstract::The DNA barcoding technique is often used as a tool for validating species identity in biobanks. In the case of amphibians, the mitochondrial DNA (mtDNA) 16S ribosomal RNA (rRNA) gene is reported to fulfill the requirements of a universal DNA barcoding marker. The 16S primers are designed to specifically bind to the 1...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2011.0036

    authors: Maya-Soriano MJ,Holt WV,Lloyd RE

    更新日期:2012-02-01 00:00:00

  • Increased Cell Survival of Human Primary Conjunctival Stem Cells in Dimethyl Sulfoxide-Based Cryopreservation Media.

    abstract::Glycerol and dimethyl sulfoxide (DMSO) are widely used cryoprotectants for freezing human cell cultures. During the manufacturing process of ocular stem cell-based autographs, ex vivo cultivated ocular cells are cryopreserved for quality control purposes in accordance with regulatory requirements. The efficiency of th...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2020.0091

    authors: van Velthoven AJH,Bertolin M,Barbaro V,Sthijns MMJPE,Nuijts RMMA,LaPointe VLS,Dickman MM,Ferrari S

    更新日期:2020-11-13 00:00:00

  • Inactivation of Influenza A virus, Adenovirus, and Cytomegalovirus with PAXgene tissue fixative and formalin.

    abstract::Formalin fixation is known to inactivate most viruses in a vaccine production context, but nothing is published about virus activity in tissues treated with alternative, non-crosslinking fixatives. We used a model assay based on cell culture to test formalin and PAXgene Tissue fixative for their virus-inactivating abi...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2013.0010

    authors: Kap M,Arron GI,Loibner M,Hausleitner A,Siaulyte G,Zatloukal K,Murk JL,Riegman P

    更新日期:2013-08-01 00:00:00

  • Long-Term Room Temperature Storage of Dry Ribonucleic Acid for Use in RNA-Seq Analysis.

    abstract::RNA is an essential biological material for research in genomics and translational medicine. As such, its storage for biobanking is an important field of study. Traditionally, long-term storage in the cold (generally freezers or liquid nitrogen) is used to maintain high-quality (in terms of quantity and integrity) RNA...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2017.0024

    authors: Li Q,Wang X,Liu X,Liao Q,Sun J,He X,Yang T,Yin J,Jia J,Li X,Colotte M,Bonnet J

    更新日期:2017-12-01 00:00:00

  • Landscape analysis of registries and biobanks: a tool for disease advocacy organizations to enhance translational research systems.

    abstract::Disease advocacy organizations play an increased role in the development and management of registries and biorepositories. Genetic Alliance developed criteria to assist organizations in selecting vendors and conducted a technical assessment survey of potential vendors. More than half offered customizable solutions, an...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2010.0007

    authors: Horn EJ,Bialick JF,Terry SF

    更新日期:2010-06-01 00:00:00

  • Sample Confirmation Testing: A Short Tandem Repeat-Based Quality Assurance and Quality Control Procedure for the eyeGENE Biorepository.

    abstract::Quality assurance and quality control (QA/QC) procedures are vital to good biorepository management. The National Eye Institute (NEI) core CLIA-certified laboratory of the eyeGENE(®) Network receives blood from individuals with inherited eye conditions and isolates DNA for clinical genetic diagnostic testing and resea...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2015.0098

    authors: Parrish RS,Garafalo AV,Ndifor V,Goetz KE,Reeves MJ,Yim A,Cooper RC,Iano-Fletcher J,Wang X,Tumminia SJ

    更新日期:2016-04-01 00:00:00

  • Targeting Future Customers: An Introductory Biobanking Course for Undergraduate Students of Life Sciences.

    abstract:INTRODUCTION:Biobanking is a relatively new concept in the Arab region. Targeting different stakeholders to introduce the concept of biobanking and develop an acceptance of it among them is important for the growth of biobanking in the region. Undergraduate students of life sciences represent an important segment of st...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2016.0111

    authors: Abdelhafiz AS,Fouda MA,El-Jaafary SI,Farghly MI,Salem M,Tammam A,Gabr H

    更新日期:2017-08-01 00:00:00

  • Integrating Molecular Control to Improve Cryopreservation Outcome.

    abstract::Cryopreservation (CP) is a critical component in enabling on-demand access to biological material (macromolecules, cells, and tissues), yet CP has evolved little over the last several decades. Today's CP processes often yield a suboptimal "product," which has slowed progression in areas such as cell therapy and stem c...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章,评审

    doi:10.1089/bio.2016.0119

    authors: Baust JG,Snyder KK,Van Buskirk R,Baust JM

    更新日期:2017-04-01 00:00:00

  • Cryopreservation and Culture of Testicular Tissues: An Essential Tool for Biodiversity Preservation.

    abstract::Systematic cryo-banking of reproductive tissues could enhance reproductive management and ensure sustainability of rare mammalian genotypes. Testicular tissues contain a vast number of germ cells, including at early stages (spermatogonia and spermatocytes), that can potentially develop into viable spermatozoa after gr...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2020.0010

    authors: Silva AMD,Pereira AF,Comizzoli P,Silva AR

    更新日期:2020-06-01 00:00:00

  • Effect of Synergism Between Carboxylated Poly-l-Lysine and Glycerol on Freezability of Nili-Ravi Buffalo (Bubalus bubalis) Semen.

    abstract::Carboxylated poly-l-lysine (CPLL), an ampholytic polymer, has remarkable cryoprotective properties. It was hypothesized that CPLL will reduce/replace glycerol in extender and improve the freezability of buffalo semen. The objective was to evaluate various combinations of CPLL and glycerol in extenders for any synergis...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2019.0120

    authors: Akhter S,Awan MA,Arshad J,Rakha BA,Ansari MS,Iqbal S

    更新日期:2020-10-01 00:00:00

  • Don't Take It Personal: European Union Legal Aspects of Procuring and Protecting Environmental Exposure Data in Population Biobanks Through the Use of a Geo-Information-Systems Toolkit.

    abstract::Under European Union (EU) law, population-based cohort studies have the right to collect environmental data and to access geospatial data, at street level, on the web, from a host of public sources. As to geospatial information, they should be able to avail themselves of Member States' networks of services for geospat...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2016.0007

    authors: Bovenberg JA,de Hoogh K,Knoppers BM,Hveem K,Hansell AL

    更新日期:2016-06-01 00:00:00

  • How Semantics Connotations May Influence Concerns About Donation of Biospecimens.

    abstract:: Introduction: Human biological specimen (biospecimen) donation is routinely requested for clinical care and research purposes. Successfully engaging patients and research participants in biospecimen donation depends on what they understand these initiatives entail, includi...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2020.0072

    authors: Page SA,Collisson BA,Godley J,Nguyen D,Metz L,Muruve D

    更新日期:2020-11-11 00:00:00

  • Models of biobanking and tissue preservation: RNA quality in archival samples in pathology laboratories and "in vivo biobanking" by tumor xenografts in nude mice-two models of quality assurance in pathology.

    abstract::Tissue banks represent essential resources and platforms for biomedical research serving basic, translational, and clinical research projects. In this article, we describe 2 models of biobanking and tissue preservation with different approaches and aims. Archive tissue biobanking is described here as a resource of res...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2011.0010

    authors: Stanta G,Bonin S,Machado I,Llombart-Bosch A

    更新日期:2011-06-01 00:00:00

  • Enhanced Cryoprotective Effect of Melatonin and Resveratrol by Coencapsulation: Improved In Vitro Development of Vitrified-Warmed Mouse Germinal Vesicle Oocytes.

    abstract::Oocyte vitrification, as a vital step in reproductive medicine, is strongly associated with lower development caused by cryodamaging factors, such as oxidative stress. In this study, we evaluated the antioxidative synergistic effects of Melatonin (Mel) and Resveratrol (RES) coencapsulated by solid lipid nanocarriers (...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2020.0102

    authors: Aghaz F,Vaisi-Raygani A,Khazaei M,Arkan E

    更新日期:2020-12-22 00:00:00

  • A review of international biobanks and networks: success factors and key benchmarks.

    abstract::Biobanks and biobanking networks are involved in varying degrees in the collection, processing, storage, and dissemination of biological specimens. This review outlines the approaches that 16 of the largest biobanks and biobanking networks in Europe, North America, Australia, and Asia have taken to collecting and dist...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2010.0003

    authors: Vaught J,Kelly A,Hewitt R

    更新日期:2009-09-01 00:00:00

  • Mesenchymal stem cell isolation from human umbilical cord tissue: understanding and minimizing variability in cell yield for process optimization.

    abstract::Human tissue banks are a potential source of cellular material for the nascent cell-based therapy industry; umbilical cord (UC) tissue is increasingly privately banked in such facilities as a source of mesenchymal stem cells for future therapeutic use. However, early handling of UC tissue is relatively uncontrolled du...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2013.0027

    authors: Iftimia-Mander A,Hourd P,Dainty R,Thomas RJ

    更新日期:2013-10-01 00:00:00

  • The Montpellier Leishmania Collection, from a Laboratory Collection to a Biological Resource Center: A 39-Year-Long Story.

    abstract::We report the development of a laboratory collection of Leishmania that was initiated in 1975 and, after 39 years, has become an international Biological Resource Center (BRC-Leish, Montpellier, France, BioBank No. BB-0033-00052), which includes 6353 strains belonging to 36 Leishmania taxa. This is a retrospective ana...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2015.0101

    authors: Pratlong F,Balard Y,Lami P,Talignani L,Ravel C,Dereure J,Lefebvre M,Serres G,Bastien P,Dedet JP

    更新日期:2016-12-01 00:00:00

  • Biorepository for Pediatric Cancer with Minimal Resources: Meeting the Challenges.

    abstract:BACKGROUND:Conducting high throughput -omics research requires high quality, data-rich biospecimens to unravel factors underlying childhood cancers; this is an extra burden in a limited resources country. For this purpose, Children's Cancer Hospital (CCHE), the largest pediatric cancer hospital worldwide, established a...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2015.0004

    authors: Labib RM,Mostafa MM,Alfaar AS,Yehia D,Alaa M,Elzayat MG,Adel M,Ezzat S,Abo El-Naga S

    更新日期:2016-02-01 00:00:00

  • Development of pancreas storage solutions: Initial screening of cytoprotective supplements for β-cell survival and metabolic status after hypothermic storage.

    abstract::Insulin-dependent diabetes mellitus is one of the leading causes of death world-wide. Donor-derived pancreas and Islet of Langerhans transplantation are potential cures; however, postmortem ischemia impacts islet quality. The murine βt3 cell line was employed as a model to study cell viability and proliferation after ...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2012.0023

    authors: Campbell LH,Taylor MJ,Brockbank KG

    更新日期:2013-02-01 00:00:00

  • The use of biospecimens in population-based research: a review of the National Cancer Institute's Division of Cancer Control and Population Sciences grant portfolio.

    abstract::Over the past two decades, researchers have increasingly used human biospecimens to evaluate hypotheses related to disease risk, outcomes and treatment. We conducted an analysis of population-science cancer research grants funded by the National Cancer Institute (NCI) to gain a more comprehensive understanding of bios...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章,评审

    doi:10.1089/bio.2014.0009

    authors: Carrick DM,Mette E,Hoyle B,Rogers SD,Gillanders EM,Schully SD,Mechanic LE

    更新日期:2014-08-01 00:00:00

  • Changing the Culture of Tumor Tissue Biobanking in a Tertiary Referral Hospital Using an Audit and Feedback Strategy.

    abstract::We examined whether the introduction of an audit and feedback strategy applied to specimens dissected at the John Hunter Hospital anatomical pathology department improved the number of formalin fixed tumor specimens sampled by anatomical pathology registrars for the Hunter Cancer Biobank. During the audit period (1/7/...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2018.0100

    authors: King S,Walker M,Neilsen S,O'Brien M

    更新日期:2019-01-01 00:00:00