Abstract:
:The advent of molecular characterization of tissues has brought an increasing emphasis on the quality of biospecimens, starting with the tissue procurement process. RNA levels are particularly affected by factors in the collection process, but the influence of different pre-analytical factors is not well understood. Here we present the influence of tissue specimen size, as well as the transport and freezing protocols, on RNA quality. Large, medium, and smaller porcine liver samples were stored either dry, on moist gauze, or in salt solution for various times, and then frozen in either liquid nitrogen or in pre-cooled isopentane. Large and small human liver samples were frozen in pre-cooled isopentane either immediately or after one hour at room temperature. The small samples were stored dry, on moist gauze, or in salt solution. RNA was isolated and RIN values were measured. The RNA for six standard reference genes from human liver was analyzed by RT-qPCR, and tissue morphology was assessed for artifacts of freezing. Experiments using porcine liver samples showed that RNA derived from smaller samples was more degraded after one hour of cold ischemia, and that cooled transport is preferable. Human liver samples showed significant RNA degradation after 1 h of cold ischemia, which was more pronounced in smaller samples. RNA integrity was not significantly influenced by the transport or freezing method, but changes in gene expression were observed in samples either transported on gauze or in salt solution. Based on observations in liver samples, smaller samples are more subject to gene expression variability introduced by post-excision sample handling than are larger samples. Small biopsies should be transported on ice and snap frozen as soon as possible after acquisition from the patient.
journal_name
Biopreserv Biobankjournal_title
Biopreservation and biobankingauthors
Kap M,Sieuwerts AM,Kubista M,Oomen M,Arshad S,Riegman Pdoi
10.1089/bio.2014.0076subject
Has Abstractpub_date
2015-06-01 00:00:00pages
200-6issue
3eissn
1947-5535issn
1947-5543journal_volume
13pub_type
杂志文章abstract::Adipose tissue, which is harvested in large quantities during liposuction, has no blood supply and becomes necrotic within a few hours, if not immediately transplanted. Cryopreservation of adipose tissue allows these samples to be stored and used in diverse fundamental experiments, especially in fat-grafting animal te...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0038
更新日期:2020-10-09 00:00:00
abstract::Systematic cryo-banking of reproductive tissues could enhance reproductive management and ensure sustainability of rare mammalian genotypes. Testicular tissues contain a vast number of germ cells, including at early stages (spermatogonia and spermatocytes), that can potentially develop into viable spermatozoa after gr...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0010
更新日期:2020-06-01 00:00:00
abstract::Cryopreservation, which refers to preservation of cells or tissues at subzero temperatures, inevitably involves the problem of cryoinjury caused by ice crystals. The application of an external electric field during the freezing process has been shown to be a promising approach to produce miniature ice grains and decre...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0045
更新日期:2020-10-16 00:00:00
abstract::Quality assurance and quality control (QA/QC) procedures are vital to good biorepository management. The National Eye Institute (NEI) core CLIA-certified laboratory of the eyeGENE(®) Network receives blood from individuals with inherited eye conditions and isolates DNA for clinical genetic diagnostic testing and resea...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0098
更新日期:2016-04-01 00:00:00
abstract:: Introduction: Human biological specimen (biospecimen) donation is routinely requested for clinical care and research purposes. Successfully engaging patients and research participants in biospecimen donation depends on what they understand these initiatives entail, includi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0072
更新日期:2020-11-11 00:00:00
abstract::Due to the requirement for comprehensive clinical research efforts in China, the importance of biobanking in modern clinical research is outlined in this overview. Hospitals, universities, and research institutes have been well organized as fundamental resources for Chinese biobanking initiatives and the resulting bio...
journal_title:Biopreservation and biobanking
pub_type: 历史文章,杂志文章
doi:10.1089/bio.2014.0096
更新日期:2015-02-01 00:00:00
abstract::Cryopreservation includes a set of techniques aimed at storing biological samples and preserving their biochemical and functional features without any significant alterations. This study set out to investigate the effects induced by cryopreservation on cultured sheepskin fibroblasts (CSSF) through cryomicroscopy and g...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0100
更新日期:2017-08-01 00:00:00
abstract:INTRODUCTION:Biobanking is a relatively new concept in the Arab region. Targeting different stakeholders to introduce the concept of biobanking and develop an acceptance of it among them is important for the growth of biobanking in the region. Undergraduate students of life sciences represent an important segment of st...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0111
更新日期:2017-08-01 00:00:00
abstract::Biobank-suisse (BBS) is a collaborative network of biobanks in Switzerland. Since 2005, the network has worked with biobank managers towards a Swiss biobanking platform that harmonizes structures and procedures. The work with biobank managers has shown that long-term, sustainable financing is difficult to obtain. In t...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0057
更新日期:2014-12-01 00:00:00
abstract::Handling and processing of blood can significantly alter the molecular composition and consistency of biobank samples and can have a major impact on the identification of biomarkers. It is thus crucial to identify tools to determine the quality of samples to be used in biomarker discovery studies. In this study, a non...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0092
更新日期:2016-10-01 00:00:00
abstract::Bacterial contamination often occurs in human blood DNA samples, possibly due to bacteremia or an inappropriate procedure during sample preparation. This study aimed at analyzing the clinical significance of bacterial DNA contamination in human blood DNA samples and to assess its influence on experimental data. DNA sa...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2010.0012
更新日期:2010-09-01 00:00:00
abstract::Over the past two decades, researchers have increasingly used human biospecimens to evaluate hypotheses related to disease risk, outcomes and treatment. We conducted an analysis of population-science cancer research grants funded by the National Cancer Institute (NCI) to gain a more comprehensive understanding of bios...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2014.0009
更新日期:2014-08-01 00:00:00
abstract::Cryopreservation is a recognized method for the maintenance of Leptospira collections. Although cryoprotectants are commonly used in order to prevent or reduce the adverse effects of freezing, there is no consensus regarding the protocols of cryopreservation. This study aimed to compare cryopreservation protocols for ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0046
更新日期:2016-02-01 00:00:00
abstract::The social structures that govern data/sample release aim to safeguard the confidentiality and privacy of cohort research participants (without whom there would be no data or samples) and enable the realization of societal benefit through optimizing the scientific use of those cohorts. Within collaborations involving ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0002
更新日期:2016-06-01 00:00:00
abstract::In Poland storage of human biological samples takes place at most universities and scientific institutions conducting research projects in the field of biomedicine. The First International Biobanking Conference organized by the Ministry of Science and Higher Education in 2014 shed a light on the situation of Polish bi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0091
更新日期:2017-06-01 00:00:00
abstract::The challenges facing biobanks are changing from simple collections of materials to quality-assured fit-for-purpose clinically annotated samples. As a result, informatics awareness and capabilities of a biobank are now intrinsically related to quality. A biobank may be considered a data repository, in the form of raw ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0099
更新日期:2015-10-01 00:00:00
abstract::The aim of this study was to evaluate the efficiency of different media in the in vitro culture of bovine preantral follicles that were used either fresh or following slow freezing treatment. Frozen and fresh noncultured or cultured ovarian fragments were processed for histological, viability, and cell proliferation a...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0020
更新日期:2014-10-01 00:00:00
abstract::This is a two-part review describing the planning, engineering, and design considerations for building a new repository for biological and environmental samples. Part I addresses the physical infrastructure requirements for a repository; Part II will cover equipment and costing. Planning for construction of a new repo...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2015.0084
更新日期:2016-04-01 00:00:00
abstract::Glycerol and dimethyl sulfoxide (DMSO) are widely used cryoprotectants for freezing human cell cultures. During the manufacturing process of ocular stem cell-based autographs, ex vivo cultivated ocular cells are cryopreserved for quality control purposes in accordance with regulatory requirements. The efficiency of th...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0091
更新日期:2020-11-13 00:00:00
abstract::The consent process involves three steps; referral for contact, preliminary interview, and informed consent discussion. We propose that the efficiency and frequency of the consent process for individual biobank related projects increases when the referral for contact is conducted by an independent "Permission to Conta...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0004
更新日期:2013-06-01 00:00:00
abstract::RNA is an essential biological material for research in genomics and translational medicine. As such, its storage for biobanking is an important field of study. Traditionally, long-term storage in the cold (generally freezers or liquid nitrogen) is used to maintain high-quality (in terms of quantity and integrity) RNA...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2017.0024
更新日期:2017-12-01 00:00:00
abstract::It is particularly necessary for biomedical researchers to obtain applicable biosamples accurately and efficiently, especially from a biobank with multiple-disease catalogs. To optimize the retrieval procedure, especially in the early stages of a non-automatic biobank, we developed a procedure that combined the electr...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0102
更新日期:2015-02-01 00:00:00
abstract::We report the development of a laboratory collection of Leishmania that was initiated in 1975 and, after 39 years, has become an international Biological Resource Center (BRC-Leish, Montpellier, France, BioBank No. BB-0033-00052), which includes 6353 strains belonging to 36 Leishmania taxa. This is a retrospective ana...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0101
更新日期:2016-12-01 00:00:00
abstract::Formalin fixation is known to inactivate most viruses in a vaccine production context, but nothing is published about virus activity in tissues treated with alternative, non-crosslinking fixatives. We used a model assay based on cell culture to test formalin and PAXgene Tissue fixative for their virus-inactivating abi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0010
更新日期:2013-08-01 00:00:00
abstract:BACKGROUND:Conducting high throughput -omics research requires high quality, data-rich biospecimens to unravel factors underlying childhood cancers; this is an extra burden in a limited resources country. For this purpose, Children's Cancer Hospital (CCHE), the largest pediatric cancer hospital worldwide, established a...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0004
更新日期:2016-02-01 00:00:00
abstract::The Beaumont Health System BioBank was established in 2008, not only to leverage the potential to collect biospecimens for translational research, but to undertake such research in a seamless facility that combined high quality biobanking with state-of-the-art laboratory platforms geared towards biospecimen-based rese...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0049
更新日期:2014-10-01 00:00:00
abstract::Disease advocacy organizations play an increased role in the development and management of registries and biorepositories. Genetic Alliance developed criteria to assist organizations in selecting vendors and conducted a technical assessment survey of potential vendors. More than half offered customizable solutions, an...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2010.0007
更新日期:2010-06-01 00:00:00
abstract::Biobanking at Duke University has existed for decades and has grown over time in silos and based on specialized needs, as is true with most biomedical research centers. These silos developed informatics systems to support their own individual requirements, with no regard for semantic or syntactic interoperability. Duk...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0092
更新日期:2017-04-01 00:00:00
abstract::Following its emergence in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused what rapidly became a global pandemic. The precise origin and subsequent path of transmission have not yet been established-but like the other novel coronaviruses that it closely resembles, it appears to have ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2020.0066
更新日期:2020-12-01 00:00:00
abstract::We examined whether the introduction of an audit and feedback strategy applied to specimens dissected at the John Hunter Hospital anatomical pathology department improved the number of formalin fixed tumor specimens sampled by anatomical pathology registrars for the Hunter Cancer Biobank. During the audit period (1/7/...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2018.0100
更新日期:2019-01-01 00:00:00