Abstract:
:Insulin-dependent diabetes mellitus is one of the leading causes of death world-wide. Donor-derived pancreas and Islet of Langerhans transplantation are potential cures; however, postmortem ischemia impacts islet quality. The murine βt3 cell line was employed as a model to study cell viability and proliferation after hypothermic storage by comparing Belzer's Machine Perfusion Solution with Unisol™ Solution. The objective was to determine which of these solutions provided the best base line support for βt3 cells and to screen potential cytoprotective additives to the solutions. Initial βt3 cell viability was similar in the two storage solutions; however, better proliferation was observed after storage in Unisol Solution. The caspase inhibitor, Q-VD-OPH, and α-tocopherol improved viability in both storage solutions, suggesting that apoptotic pathways may be responsible for cell death during hypothermic storage of βt3 cells. Analysis of apoptosis markers, caspase activity, and DNA laddering showed a reduction in apoptosis when these additives were included. The effects of Q-VD-OPH and α-tocopherol were also synergistic when employed together during either hypothermic exposure, post-hypothermic physiologic incubation, or combinations of hypothermic exposure and physiologic incubation. These results suggest that both supplements should be included in pancreas hypothermic storage solutions and in islet culture media during post-isolation culture prior to transplantation.
journal_name
Biopreserv Biobankjournal_title
Biopreservation and biobankingauthors
Campbell LH,Taylor MJ,Brockbank KGdoi
10.1089/bio.2012.0023subject
Has Abstractpub_date
2013-02-01 00:00:00pages
12-8issue
1eissn
1947-5535issn
1947-5543journal_volume
11pub_type
杂志文章abstract::The consent process involves three steps; referral for contact, preliminary interview, and informed consent discussion. We propose that the efficiency and frequency of the consent process for individual biobank related projects increases when the referral for contact is conducted by an independent "Permission to Conta...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0004
更新日期:2013-06-01 00:00:00
abstract::Bacterial contamination often occurs in human blood DNA samples, possibly due to bacteremia or an inappropriate procedure during sample preparation. This study aimed at analyzing the clinical significance of bacterial DNA contamination in human blood DNA samples and to assess its influence on experimental data. DNA sa...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2010.0012
更新日期:2010-09-01 00:00:00
abstract::Biobanks and biobanking networks are involved in varying degrees in the collection, processing, storage, and dissemination of biological specimens. This review outlines the approaches that 16 of the largest biobanks and biobanking networks in Europe, North America, Australia, and Asia have taken to collecting and dist...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2010.0003
更新日期:2009-09-01 00:00:00
abstract:: Introduction: Human biological specimen (biospecimen) donation is routinely requested for clinical care and research purposes. Successfully engaging patients and research participants in biospecimen donation depends on what they understand these initiatives entail, includi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0072
更新日期:2020-11-11 00:00:00
abstract::Tissues from postmortem transplantation donors are a viable and productive option for genomic research. This entails obtaining authorization from the family decision makers (FDMs) of deceased donors. This study examined best practices for making such requests within the context of the Genotype-Tissue Expression (GTEx)...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2017.0115
更新日期:2018-06-01 00:00:00
abstract:INTRODUCTION:Biobanking is a relatively new concept in the Arab region. Targeting different stakeholders to introduce the concept of biobanking and develop an acceptance of it among them is important for the growth of biobanking in the region. Undergraduate students of life sciences represent an important segment of st...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0111
更新日期:2017-08-01 00:00:00
abstract::The Beaumont Health System BioBank was established in 2008, not only to leverage the potential to collect biospecimens for translational research, but to undertake such research in a seamless facility that combined high quality biobanking with state-of-the-art laboratory platforms geared towards biospecimen-based rese...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0049
更新日期:2014-10-01 00:00:00
abstract::We examined whether the introduction of an audit and feedback strategy applied to specimens dissected at the John Hunter Hospital anatomical pathology department improved the number of formalin fixed tumor specimens sampled by anatomical pathology registrars for the Hunter Cancer Biobank. During the audit period (1/7/...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2018.0100
更新日期:2019-01-01 00:00:00
abstract::Human tissue banks are a potential source of cellular material for the nascent cell-based therapy industry; umbilical cord (UC) tissue is increasingly privately banked in such facilities as a source of mesenchymal stem cells for future therapeutic use. However, early handling of UC tissue is relatively uncontrolled du...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0027
更新日期:2013-10-01 00:00:00
abstract::Glycerol and dimethyl sulfoxide (DMSO) are widely used cryoprotectants for freezing human cell cultures. During the manufacturing process of ocular stem cell-based autographs, ex vivo cultivated ocular cells are cryopreserved for quality control purposes in accordance with regulatory requirements. The efficiency of th...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0091
更新日期:2020-11-13 00:00:00
abstract::Biobanking at Duke University has existed for decades and has grown over time in silos and based on specialized needs, as is true with most biomedical research centers. These silos developed informatics systems to support their own individual requirements, with no regard for semantic or syntactic interoperability. Duk...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0092
更新日期:2017-04-01 00:00:00
abstract::Modern taxonomy requires the preservation of biospecimens for both morphological and molecular applications. The utility of a previously identified preservative, dimethyldimethylhydantoin hydantoin (Dekafald®), to retain both physical diagnostic traits and the DNA integrity of biological specimens remains unknown. Usi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0001
更新日期:2020-08-01 00:00:00
abstract::The aim of this study was to evaluate the efficiency of different media in the in vitro culture of bovine preantral follicles that were used either fresh or following slow freezing treatment. Frozen and fresh noncultured or cultured ovarian fragments were processed for histological, viability, and cell proliferation a...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0020
更新日期:2014-10-01 00:00:00
abstract::The advent of molecular characterization of tissues has brought an increasing emphasis on the quality of biospecimens, starting with the tissue procurement process. RNA levels are particularly affected by factors in the collection process, but the influence of different pre-analytical factors is not well understood. H...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0076
更新日期:2015-06-01 00:00:00
abstract::The COVID-19 era has brought about a number of novel challenges for the global biobanking community. An array of diverse tools (e.g., standards, best practices, and plans) exists to support quality and fitness-for-purpose in biobank operations. The International Society for Biological and Environmental Repositories (I...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0083
更新日期:2020-12-01 00:00:00
abstract::The social structures that govern data/sample release aim to safeguard the confidentiality and privacy of cohort research participants (without whom there would be no data or samples) and enable the realization of societal benefit through optimizing the scientific use of those cohorts. Within collaborations involving ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0002
更新日期:2016-06-01 00:00:00
abstract:BACKGROUND:The preanalytical phase is considered the most vulnerable phase in biopreservation, biobanking, and laboratory diagnostics. Accurate mixing after blood collection is claimed to be important and recommended by the manufacturers. OBJECTIVE:To evaluate whether it is really necessary to mix the primary blood tu...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0043
更新日期:2014-02-01 00:00:00
abstract::Formalin fixation is known to inactivate most viruses in a vaccine production context, but nothing is published about virus activity in tissues treated with alternative, non-crosslinking fixatives. We used a model assay based on cell culture to test formalin and PAXgene Tissue fixative for their virus-inactivating abi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0010
更新日期:2013-08-01 00:00:00
abstract::Oocyte vitrification, as a vital step in reproductive medicine, is strongly associated with lower development caused by cryodamaging factors, such as oxidative stress. In this study, we evaluated the antioxidative synergistic effects of Melatonin (Mel) and Resveratrol (RES) coencapsulated by solid lipid nanocarriers (...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0102
更新日期:2020-12-22 00:00:00
abstract::Research infrastructures remain the key for state-of-the-art and successful research. In the last few decades, biobanks have become increasingly important in this field through standardization of biospecimen processing, sample storage, and standardized data management. Research infrastructure in cohort studies and oth...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0087
更新日期:2015-12-01 00:00:00
abstract::Due to the requirement for comprehensive clinical research efforts in China, the importance of biobanking in modern clinical research is outlined in this overview. Hospitals, universities, and research institutes have been well organized as fundamental resources for Chinese biobanking initiatives and the resulting bio...
journal_title:Biopreservation and biobanking
pub_type: 历史文章,杂志文章
doi:10.1089/bio.2014.0096
更新日期:2015-02-01 00:00:00
abstract::Tissue banks represent essential resources and platforms for biomedical research serving basic, translational, and clinical research projects. In this article, we describe 2 models of biobanking and tissue preservation with different approaches and aims. Archive tissue biobanking is described here as a resource of res...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2011.0010
更新日期:2011-06-01 00:00:00
abstract::The biobanking literature frequently addresses donor and societal issues surrounding biobanking, but the biobanker's perspective is rarely highlighted. While not comprehensive, this article offers an overview of the human aspects of biobanking from the viewpoint of biobank personnel-from biobank formation, through the...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2011.0018
更新日期:2011-09-01 00:00:00
abstract::Quality assurance and quality control (QA/QC) procedures are vital to good biorepository management. The National Eye Institute (NEI) core CLIA-certified laboratory of the eyeGENE(®) Network receives blood from individuals with inherited eye conditions and isolates DNA for clinical genetic diagnostic testing and resea...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0098
更新日期:2016-04-01 00:00:00
abstract::Biobanking biological samples involve multiple handling, processing, and labeling steps. Each step may be a source of error, which if unnoticed or uncorrected may have consequences for research. We aimed to develop a simple and inexpensive genotyping method that would be valuable to detect such errors and confirm samp...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0113
更新日期:2016-10-01 00:00:00
abstract::The quality of RNA preserved in different stabilization matrices was investigated after 2 weeks of storage at room temperature. RNA samples in RNAstable (Biomatrica), GenTegra (IntegenX), and RNAshell (Imagene) were compared to RNA stored at -80°C (the current gold standard for RNA preservation) and with liquid or dri...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,多中心研究
doi:10.1089/bio.2012.0030
更新日期:2012-12-01 00:00:00
abstract::Most university biobanks begin like other university research projects, that is, with an idea conceived by an individual researcher in pursuit of his/her own research interests, publications, funding, and career. Some biobanks, however, come to have scientific value that goes beyond the projects that were initially re...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2018.0054
更新日期:2018-08-01 00:00:00
abstract::A paucity of information exists on the recruitment of Asian Americans for biospecimen research. Although studies show that Chinese Americans are at high risk for hepatitis B virus (HBV) infection, little is known about their willingness to participate in HBV-related biospecimen research and how knowledge, attitudes, a...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0081
更新日期:2014-04-01 00:00:00
abstract::Adipose tissue, which is harvested in large quantities during liposuction, has no blood supply and becomes necrotic within a few hours, if not immediately transplanted. Cryopreservation of adipose tissue allows these samples to be stored and used in diverse fundamental experiments, especially in fat-grafting animal te...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0038
更新日期:2020-10-09 00:00:00
abstract::The establishment of a cryopreservation procedure for sheep oocytes will enhance the development of long-term conservation of sheep female genetic resources and the advancement of sheep embryonic biotechnology. However, higher cytoplasmic lipid content and complex subcellular structures affect the relatively higher se...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2017.0074
更新日期:2017-12-01 00:00:00