Abstract:
:Cryopreservation (CP) is a critical component in enabling on-demand access to biological material (macromolecules, cells, and tissues), yet CP has evolved little over the last several decades. Today's CP processes often yield a suboptimal "product," which has slowed progression in areas such as cell therapy and stem cell research. Recent discoveries focusing on molecular control and buffering of cell stress responses to CP as well as the development of new devices that improve sample freezing and thawing are now providing a path to improve sample quality. Numerous reports now identify the problems associated with CP-induced delayed-onset cell death (CIDOCD), with a focus on the mitigation of cell stress responses through freeze media formulation to optimize survival during "cold chain" sample management. Importantly, these new approaches that manage cell stress response are now providing a basis for advancements in cell therapy development. Herein, we provide an overview of the molecular stress responses of cells to CP, including the impact of hypothermic and cell death continuums and the strategies for improving preservation outcome.
journal_name
Biopreserv Biobankjournal_title
Biopreservation and biobankingauthors
Baust JG,Snyder KK,Van Buskirk R,Baust JMdoi
10.1089/bio.2016.0119subject
Has Abstractpub_date
2017-04-01 00:00:00pages
134-141issue
2eissn
1947-5535issn
1947-5543journal_volume
15pub_type
杂志文章,评审abstract::The past 15 years has seen considerable changes in the research environment. These changes include the development of new sophisticated genetic and genomic technologies, a proliferation of databases containing large amount of genotypic and phenotypic data, and wide-spread data sharing among many institutions, national...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2017.0030
更新日期:2017-04-01 00:00:00
abstract::Systematic cryo-banking of reproductive tissues could enhance reproductive management and ensure sustainability of rare mammalian genotypes. Testicular tissues contain a vast number of germ cells, including at early stages (spermatogonia and spermatocytes), that can potentially develop into viable spermatozoa after gr...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0010
更新日期:2020-06-01 00:00:00
abstract::The social structures that govern data/sample release aim to safeguard the confidentiality and privacy of cohort research participants (without whom there would be no data or samples) and enable the realization of societal benefit through optimizing the scientific use of those cohorts. Within collaborations involving ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0002
更新日期:2016-06-01 00:00:00
abstract::Biobanking biological samples involve multiple handling, processing, and labeling steps. Each step may be a source of error, which if unnoticed or uncorrected may have consequences for research. We aimed to develop a simple and inexpensive genotyping method that would be valuable to detect such errors and confirm samp...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0113
更新日期:2016-10-01 00:00:00
abstract::Under European Union (EU) law, population-based cohort studies have the right to collect environmental data and to access geospatial data, at street level, on the web, from a host of public sources. As to geospatial information, they should be able to avail themselves of Member States' networks of services for geospat...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0007
更新日期:2016-06-01 00:00:00
abstract::Bacterial contamination often occurs in human blood DNA samples, possibly due to bacteremia or an inappropriate procedure during sample preparation. This study aimed at analyzing the clinical significance of bacterial DNA contamination in human blood DNA samples and to assess its influence on experimental data. DNA sa...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2010.0012
更新日期:2010-09-01 00:00:00
abstract::Most university biobanks begin like other university research projects, that is, with an idea conceived by an individual researcher in pursuit of his/her own research interests, publications, funding, and career. Some biobanks, however, come to have scientific value that goes beyond the projects that were initially re...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2018.0054
更新日期:2018-08-01 00:00:00
abstract::l-Carnitine (LC) plays a key role in sperm metabolism, easily providing energy through β-oxidation, which positively affects motility. The objective of this study was to investigate the association between blood plasma and seminal plasma LC levels, as well as the effect of LC as an additive in a skimmed milk-based ext...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0025
更新日期:2020-10-01 00:00:00
abstract::Biobanks are now in the spotlight as key enablers supporting preclinical, clinical, and environmental research. Awareness of their value has increased along with the need for these infrastructures to be sustained through business-focused practices. Following our 2017 pilot survey on biobank business planning, we initi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2019.0005
更新日期:2019-06-01 00:00:00
abstract::Sharing data in biomedical contexts has become increasingly relevant, but privacy concerns set constraints for free sharing of individual-level data. Data protection law protects only data relating to an identifiable individual, whereas "anonymous" data are free to be used by everybody. Usage of many terms related to ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0100
更新日期:2016-10-01 00:00:00
abstract::The consent process involves three steps; referral for contact, preliminary interview, and informed consent discussion. We propose that the efficiency and frequency of the consent process for individual biobank related projects increases when the referral for contact is conducted by an independent "Permission to Conta...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0004
更新日期:2013-06-01 00:00:00
abstract::Cryopreservation includes a set of techniques aimed at storing biological samples and preserving their biochemical and functional features without any significant alterations. This study set out to investigate the effects induced by cryopreservation on cultured sheepskin fibroblasts (CSSF) through cryomicroscopy and g...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0100
更新日期:2017-08-01 00:00:00
abstract:: Introduction: Human biological specimen (biospecimen) donation is routinely requested for clinical care and research purposes. Successfully engaging patients and research participants in biospecimen donation depends on what they understand these initiatives entail, includi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0072
更新日期:2020-11-11 00:00:00
abstract:BACKGROUND:Conducting high throughput -omics research requires high quality, data-rich biospecimens to unravel factors underlying childhood cancers; this is an extra burden in a limited resources country. For this purpose, Children's Cancer Hospital (CCHE), the largest pediatric cancer hospital worldwide, established a...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0004
更新日期:2016-02-01 00:00:00
abstract::Following its emergence in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused what rapidly became a global pandemic. The precise origin and subsequent path of transmission have not yet been established-but like the other novel coronaviruses that it closely resembles, it appears to have ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2020.0066
更新日期:2020-12-01 00:00:00
abstract::Glycerol and dimethyl sulfoxide (DMSO) are widely used cryoprotectants for freezing human cell cultures. During the manufacturing process of ocular stem cell-based autographs, ex vivo cultivated ocular cells are cryopreserved for quality control purposes in accordance with regulatory requirements. The efficiency of th...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0091
更新日期:2020-11-13 00:00:00
abstract::The quality of RNA preserved in different stabilization matrices was investigated after 2 weeks of storage at room temperature. RNA samples in RNAstable (Biomatrica), GenTegra (IntegenX), and RNAshell (Imagene) were compared to RNA stored at -80°C (the current gold standard for RNA preservation) and with liquid or dri...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,多中心研究
doi:10.1089/bio.2012.0030
更新日期:2012-12-01 00:00:00
abstract::Disease advocacy organizations play an increased role in the development and management of registries and biorepositories. Genetic Alliance developed criteria to assist organizations in selecting vendors and conducted a technical assessment survey of potential vendors. More than half offered customizable solutions, an...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2010.0007
更新日期:2010-06-01 00:00:00
abstract::Cryopreservation, which refers to preservation of cells or tissues at subzero temperatures, inevitably involves the problem of cryoinjury caused by ice crystals. The application of an external electric field during the freezing process has been shown to be a promising approach to produce miniature ice grains and decre...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0045
更新日期:2020-10-16 00:00:00
abstract:BACKGROUND:Biopsies extracted from brain cancer patients often display degraded ribosomal RNA, which makes them unusable in transcriptomic experiments. This has not been properly documented in previous works aimed at refining the molecular classification of brain cancer. OBJECTIVE:To determine RNA integrity in a large...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0086
更新日期:2016-04-01 00:00:00
abstract::The Beaumont Health System BioBank was established in 2008, not only to leverage the potential to collect biospecimens for translational research, but to undertake such research in a seamless facility that combined high quality biobanking with state-of-the-art laboratory platforms geared towards biospecimen-based rese...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0049
更新日期:2014-10-01 00:00:00
abstract::Tissue banks represent essential resources and platforms for biomedical research serving basic, translational, and clinical research projects. In this article, we describe 2 models of biobanking and tissue preservation with different approaches and aims. Archive tissue biobanking is described here as a resource of res...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2011.0010
更新日期:2011-06-01 00:00:00
abstract::It is particularly necessary for biomedical researchers to obtain applicable biosamples accurately and efficiently, especially from a biobank with multiple-disease catalogs. To optimize the retrieval procedure, especially in the early stages of a non-automatic biobank, we developed a procedure that combined the electr...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0102
更新日期:2015-02-01 00:00:00
abstract::The ability to analyze cryopreserved peripheral blood mononuclear cells (PBMCs) from biobanks for antigen-specific T-cell immunity is necessary to evaluate responses to immune-based therapies. Comprehensive studies have demonstrated that the quality of frozen PBMCs is critical and the maintenance of cell viability and...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2010.0033
更新日期:2011-09-01 00:00:00
abstract::Modern taxonomy requires the preservation of biospecimens for both morphological and molecular applications. The utility of a previously identified preservative, dimethyldimethylhydantoin hydantoin (Dekafald®), to retain both physical diagnostic traits and the DNA integrity of biological specimens remains unknown. Usi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0001
更新日期:2020-08-01 00:00:00
abstract::Formalin-fixed paraffin-embedded (FFPE) material presents a readily available resource in the study of various biomarkers. There has been interest in whether the storage period has significant effect on the extracted macromolecules. Thus, in this study, we investigated if the storage period had an effect on the quanti...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2012.0052
更新日期:2013-04-01 00:00:00
abstract::The biobanking literature frequently addresses donor and societal issues surrounding biobanking, but the biobanker's perspective is rarely highlighted. While not comprehensive, this article offers an overview of the human aspects of biobanking from the viewpoint of biobank personnel-from biobank formation, through the...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2011.0018
更新日期:2011-09-01 00:00:00
abstract::We describe here a unique approach to identifying small molecules that are useful in stabilizing biomolecules in the dry and liquid states. Using biostability screens aided by in silico docking experiments and synthetic chemistry, libraries of biostability molecules are analyzed for their ability to protect important ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2012.0022
更新日期:2012-08-01 00:00:00
abstract::Formalin fixation is known to inactivate most viruses in a vaccine production context, but nothing is published about virus activity in tissues treated with alternative, non-crosslinking fixatives. We used a model assay based on cell culture to test formalin and PAXgene Tissue fixative for their virus-inactivating abi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0010
更新日期:2013-08-01 00:00:00
abstract::We examined whether the introduction of an audit and feedback strategy applied to specimens dissected at the John Hunter Hospital anatomical pathology department improved the number of formalin fixed tumor specimens sampled by anatomical pathology registrars for the Hunter Cancer Biobank. During the audit period (1/7/...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2018.0100
更新日期:2019-01-01 00:00:00