Abstract:
:We examined whether the introduction of an audit and feedback strategy applied to specimens dissected at the John Hunter Hospital anatomical pathology department improved the number of formalin fixed tumor specimens sampled by anatomical pathology registrars for the Hunter Cancer Biobank. During the audit period (1/7/16 to 30/6/17) a total of 949 tumor specimens were sampled for the biobank compared with 393 of the previous year (1/7/15 to 30/6/16) resulting in a 141% increase in specimens biobanked. A targeted group of previously underrepresented specimen types, including brain, lung, and lymph node tumors were studied in depth to establish which specimens were sampled and which potentially biobankable specimens were "missed". In this targeted group there was a 285% increase in the number of specimens biobanked and a statistically significant (p < 0.001) increase in tumor sampling in all three specimen types over the audit period compared with the previous year. In conclusion, the introduction of an audit and feedback strategy improved tumor tissue collection for biobanking. A potential drawback of email feedback is that overfixation may occur due to administrative time lag and these specimens must be documented accordingly because some tests are dependent on an optimal fixation time. Taking extra blocks for biobanking on all potential tumor specimens with excess tumor tissue at the time of cut-up can alleviate this problem.
journal_name
Biopreserv Biobankjournal_title
Biopreservation and biobankingauthors
King S,Walker M,Neilsen S,O'Brien Mdoi
10.1089/bio.2018.0100subject
Has Abstractpub_date
2019-01-01 00:00:00pages
64-70issue
1eissn
1947-5535issn
1947-5543journal_volume
17pub_type
杂志文章abstract::Tissues from postmortem transplantation donors are a viable and productive option for genomic research. This entails obtaining authorization from the family decision makers (FDMs) of deceased donors. This study examined best practices for making such requests within the context of the Genotype-Tissue Expression (GTEx)...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2017.0115
更新日期:2018-06-01 00:00:00
abstract:: Introduction: Human biological specimen (biospecimen) donation is routinely requested for clinical care and research purposes. Successfully engaging patients and research participants in biospecimen donation depends on what they understand these initiatives entail, includi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0072
更新日期:2020-11-11 00:00:00
abstract::Formalin fixation is known to inactivate most viruses in a vaccine production context, but nothing is published about virus activity in tissues treated with alternative, non-crosslinking fixatives. We used a model assay based on cell culture to test formalin and PAXgene Tissue fixative for their virus-inactivating abi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0010
更新日期:2013-08-01 00:00:00
abstract::Formalin-fixed paraffin-embedded (FFPE) material presents a readily available resource in the study of various biomarkers. There has been interest in whether the storage period has significant effect on the extracted macromolecules. Thus, in this study, we investigated if the storage period had an effect on the quanti...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2012.0052
更新日期:2013-04-01 00:00:00
abstract::Biobanking biological samples involve multiple handling, processing, and labeling steps. Each step may be a source of error, which if unnoticed or uncorrected may have consequences for research. We aimed to develop a simple and inexpensive genotyping method that would be valuable to detect such errors and confirm samp...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0113
更新日期:2016-10-01 00:00:00
abstract::Cryopreservation, which refers to preservation of cells or tissues at subzero temperatures, inevitably involves the problem of cryoinjury caused by ice crystals. The application of an external electric field during the freezing process has been shown to be a promising approach to produce miniature ice grains and decre...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0045
更新日期:2020-10-16 00:00:00
abstract::Insulin-dependent diabetes mellitus is one of the leading causes of death world-wide. Donor-derived pancreas and Islet of Langerhans transplantation are potential cures; however, postmortem ischemia impacts islet quality. The murine βt3 cell line was employed as a model to study cell viability and proliferation after ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2012.0023
更新日期:2013-02-01 00:00:00
abstract::The DNA barcoding technique is often used as a tool for validating species identity in biobanks. In the case of amphibians, the mitochondrial DNA (mtDNA) 16S ribosomal RNA (rRNA) gene is reported to fulfill the requirements of a universal DNA barcoding marker. The 16S primers are designed to specifically bind to the 1...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2011.0036
更新日期:2012-02-01 00:00:00
abstract::Cryopreservation includes a set of techniques aimed at storing biological samples and preserving their biochemical and functional features without any significant alterations. This study set out to investigate the effects induced by cryopreservation on cultured sheepskin fibroblasts (CSSF) through cryomicroscopy and g...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0100
更新日期:2017-08-01 00:00:00
abstract::Cryopreservation is a recognized method for the maintenance of Leptospira collections. Although cryoprotectants are commonly used in order to prevent or reduce the adverse effects of freezing, there is no consensus regarding the protocols of cryopreservation. This study aimed to compare cryopreservation protocols for ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0046
更新日期:2016-02-01 00:00:00
abstract::The establishment of a cryopreservation procedure for sheep oocytes will enhance the development of long-term conservation of sheep female genetic resources and the advancement of sheep embryonic biotechnology. However, higher cytoplasmic lipid content and complex subcellular structures affect the relatively higher se...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2017.0074
更新日期:2017-12-01 00:00:00
abstract::Handling and processing of blood can significantly alter the molecular composition and consistency of biobank samples and can have a major impact on the identification of biomarkers. It is thus crucial to identify tools to determine the quality of samples to be used in biomarker discovery studies. In this study, a non...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0092
更新日期:2016-10-01 00:00:00
abstract::Bacterial contamination often occurs in human blood DNA samples, possibly due to bacteremia or an inappropriate procedure during sample preparation. This study aimed at analyzing the clinical significance of bacterial DNA contamination in human blood DNA samples and to assess its influence on experimental data. DNA sa...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2010.0012
更新日期:2010-09-01 00:00:00
abstract::Adipose tissue, which is harvested in large quantities during liposuction, has no blood supply and becomes necrotic within a few hours, if not immediately transplanted. Cryopreservation of adipose tissue allows these samples to be stored and used in diverse fundamental experiments, especially in fat-grafting animal te...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0038
更新日期:2020-10-09 00:00:00
abstract::We describe here a unique approach to identifying small molecules that are useful in stabilizing biomolecules in the dry and liquid states. Using biostability screens aided by in silico docking experiments and synthetic chemistry, libraries of biostability molecules are analyzed for their ability to protect important ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2012.0022
更新日期:2012-08-01 00:00:00
abstract::Following its emergence in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused what rapidly became a global pandemic. The precise origin and subsequent path of transmission have not yet been established-but like the other novel coronaviruses that it closely resembles, it appears to have ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2020.0066
更新日期:2020-12-01 00:00:00
abstract::Over the past two decades, researchers have increasingly used human biospecimens to evaluate hypotheses related to disease risk, outcomes and treatment. We conducted an analysis of population-science cancer research grants funded by the National Cancer Institute (NCI) to gain a more comprehensive understanding of bios...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2014.0009
更新日期:2014-08-01 00:00:00
abstract::Sharing data in biomedical contexts has become increasingly relevant, but privacy concerns set constraints for free sharing of individual-level data. Data protection law protects only data relating to an identifiable individual, whereas "anonymous" data are free to be used by everybody. Usage of many terms related to ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0100
更新日期:2016-10-01 00:00:00
abstract::The quality of RNA preserved in different stabilization matrices was investigated after 2 weeks of storage at room temperature. RNA samples in RNAstable (Biomatrica), GenTegra (IntegenX), and RNAshell (Imagene) were compared to RNA stored at -80°C (the current gold standard for RNA preservation) and with liquid or dri...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,多中心研究
doi:10.1089/bio.2012.0030
更新日期:2012-12-01 00:00:00
abstract::RNA is an essential biological material for research in genomics and translational medicine. As such, its storage for biobanking is an important field of study. Traditionally, long-term storage in the cold (generally freezers or liquid nitrogen) is used to maintain high-quality (in terms of quantity and integrity) RNA...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2017.0024
更新日期:2017-12-01 00:00:00
abstract::A paucity of information exists on the recruitment of Asian Americans for biospecimen research. Although studies show that Chinese Americans are at high risk for hepatitis B virus (HBV) infection, little is known about their willingness to participate in HBV-related biospecimen research and how knowledge, attitudes, a...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0081
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND:Conducting high throughput -omics research requires high quality, data-rich biospecimens to unravel factors underlying childhood cancers; this is an extra burden in a limited resources country. For this purpose, Children's Cancer Hospital (CCHE), the largest pediatric cancer hospital worldwide, established a...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0004
更新日期:2016-02-01 00:00:00
abstract::Biobank-suisse (BBS) is a collaborative network of biobanks in Switzerland. Since 2005, the network has worked with biobank managers towards a Swiss biobanking platform that harmonizes structures and procedures. The work with biobank managers has shown that long-term, sustainable financing is difficult to obtain. In t...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0057
更新日期:2014-12-01 00:00:00
abstract::Animal-derived products are widely used in sperm cryopreservation for their cryoprotective properties. These components, however, tend to be replaced because of sanitary risks. STEMALPHA.CRYO3 (Ref. 5617; Stem Alpha, Saint-Genis-l'Argentière, France), called "CRYO3," is a chemically defined preservation medium current...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2018.0067
更新日期:2019-01-01 00:00:00
abstract::Glycerol and dimethyl sulfoxide (DMSO) are widely used cryoprotectants for freezing human cell cultures. During the manufacturing process of ocular stem cell-based autographs, ex vivo cultivated ocular cells are cryopreserved for quality control purposes in accordance with regulatory requirements. The efficiency of th...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0091
更新日期:2020-11-13 00:00:00
abstract::Disease advocacy organizations play an increased role in the development and management of registries and biorepositories. Genetic Alliance developed criteria to assist organizations in selecting vendors and conducted a technical assessment survey of potential vendors. More than half offered customizable solutions, an...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2010.0007
更新日期:2010-06-01 00:00:00
abstract:BACKGROUND:Biopsies extracted from brain cancer patients often display degraded ribosomal RNA, which makes them unusable in transcriptomic experiments. This has not been properly documented in previous works aimed at refining the molecular classification of brain cancer. OBJECTIVE:To determine RNA integrity in a large...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0086
更新日期:2016-04-01 00:00:00
abstract::Cryopreservation (CP) is a critical component in enabling on-demand access to biological material (macromolecules, cells, and tissues), yet CP has evolved little over the last several decades. Today's CP processes often yield a suboptimal "product," which has slowed progression in areas such as cell therapy and stem c...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2016.0119
更新日期:2017-04-01 00:00:00
abstract::Oocyte vitrification, as a vital step in reproductive medicine, is strongly associated with lower development caused by cryodamaging factors, such as oxidative stress. In this study, we evaluated the antioxidative synergistic effects of Melatonin (Mel) and Resveratrol (RES) coencapsulated by solid lipid nanocarriers (...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0102
更新日期:2020-12-22 00:00:00
abstract::The ability to analyze cryopreserved peripheral blood mononuclear cells (PBMCs) from biobanks for antigen-specific T-cell immunity is necessary to evaluate responses to immune-based therapies. Comprehensive studies have demonstrated that the quality of frozen PBMCs is critical and the maintenance of cell viability and...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2010.0033
更新日期:2011-09-01 00:00:00