Abstract:
:Biobanking biological samples involve multiple handling, processing, and labeling steps. Each step may be a source of error, which if unnoticed or uncorrected may have consequences for research. We aimed to develop a simple and inexpensive genotyping method that would be valuable to detect such errors and confirm sample identity. For this purpose, seven variable number of tandem repeat (VNTR) loci were selected, analyzed by polymerase chain reaction (PCR) amplification, and organized in a PCR-based DNA profiling algorithm that proved useful to minimize the number of steps required for the procedure. Match probability calculations suggest that this method/algorithm has the potential to discriminate every participant of a biobank. As a proof of concept, the algorithm was applied on samples taken from the PROCURE Prostate Cancer Biobank. It was applied on 403 DNA samples from 101 randomly chosen patients who provided prostate tissues at surgery and blood at two to three different time points over a period of up to 7 years. A unique DNA profile requiring the analysis of no more than four VNTR loci (D16S83, D17S5, D1S80, D19S20) was successfully obtained for each of the 101 cases studied and led to the identification of two mismatches among the 403 samples evaluated (0.5% error rate). Further investigations using the same genotyping method revealed that one of the errors was due to tissue mishandling and that the other was due to tissue mislabeling. These errors, typical to the complex biobanking process, highlight the importance to implement a routine genotyping method as part of quality assurance in biobanking.
journal_name
Biopreserv Biobankjournal_title
Biopreservation and biobankingauthors
Pellerin C,McKercher G,Aprikian AG,Saad F,Lacombe L,Carmel M,Chevalier Sdoi
10.1089/bio.2015.0113subject
Has Abstractpub_date
2016-10-01 00:00:00pages
383-389issue
5eissn
1947-5535issn
1947-5543journal_volume
14pub_type
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journal_title:Biopreservation and biobanking
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doi:10.1089/bio.2017.0115
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0049
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0043
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2019.0005
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0010
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0002
更新日期:2016-06-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0045
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2014.0009
更新日期:2014-08-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章,多中心研究
doi:10.1089/bio.2012.0030
更新日期:2012-12-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0076
更新日期:2015-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1089/bio.2016.0091
更新日期:2017-06-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
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journal_title:Biopreservation and biobanking
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journal_title:Biopreservation and biobanking
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更新日期:2014-12-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2015.0084
更新日期:2016-04-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0100
更新日期:2016-10-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0111
更新日期:2017-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1089/bio.2015.0087
更新日期:2015-12-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
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更新日期:2017-12-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2018.0067
更新日期:2019-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1089/bio.2010.0033
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