Short-term stability study of RNA at room temperature.

abstract：:The advent of molecular characterization of tissues has brought an increasing emphasis on the quality of biospecimens, starting with the tissue procurement process. RNA levels are particularly affected by factors in the collection process, but the influence of different pre-analytical factors is not well understood. H...

journal_title：Biopreservation and biobanking

authors： Kap M,Sieuwerts AM,Kubista M,Oomen M,Arshad S,Riegman P

更新日期：2015-06-01 00:00:00

abstract：:Cryopreservation is a recognized method for the maintenance of Leptospira collections. Although cryoprotectants are commonly used in order to prevent or reduce the adverse effects of freezing, there is no consensus regarding the protocols of cryopreservation. This study aimed to compare cryopreservation protocols for ...

journal_title：Biopreservation and biobanking

authors： Narduche L,Hamond C,Martins GM,Medeiros MA,Lilenbaum W

更新日期：2016-02-01 00:00:00

abstract：:The DNA barcoding technique is often used as a tool for validating species identity in biobanks. In the case of amphibians, the mitochondrial DNA (mtDNA) 16S ribosomal RNA (rRNA) gene is reported to fulfill the requirements of a universal DNA barcoding marker. The 16S primers are designed to specifically bind to the 1...

journal_title：Biopreservation and biobanking

authors： Maya-Soriano MJ,Holt WV,Lloyd RE

更新日期：2012-02-01 00:00:00

abstract：BACKGROUND:Biopsies extracted from brain cancer patients often display degraded ribosomal RNA, which makes them unusable in transcriptomic experiments. This has not been properly documented in previous works aimed at refining the molecular classification of brain cancer. OBJECTIVE:To determine RNA integrity in a large...

journal_title：Biopreservation and biobanking

authors： Castells Domingo X,Ferrer-Font L,Davila M,Candiota AP,Simões RV,Fernández-Coello A,Gabarrós A,Boluda S,Barceló A,Ariño J,Arús C

更新日期：2016-04-01 00:00:00

abstract：:This is a two-part review describing the planning, engineering, and design considerations for building a new repository for biological and environmental samples. Part I addresses the physical infrastructure requirements for a repository; Part II will cover equipment and costing. Planning for construction of a new repo...

journal_title：Biopreservation and biobanking

authors： Baird PM,Gunter EW

更新日期：2016-04-01 00:00:00

abstract：:Quality assurance and quality control (QA/QC) procedures are vital to good biorepository management. The National Eye Institute (NEI) core CLIA-certified laboratory of the eyeGENE(®) Network receives blood from individuals with inherited eye conditions and isolates DNA for clinical genetic diagnostic testing and resea...

journal_title：Biopreservation and biobanking

authors： Parrish RS,Garafalo AV,Ndifor V,Goetz KE,Reeves MJ,Yim A,Cooper RC,Iano-Fletcher J,Wang X,Tumminia SJ

更新日期：2016-04-01 00:00:00

abstract：:Sharing data in biomedical contexts has become increasingly relevant, but privacy concerns set constraints for free sharing of individual-level data. Data protection law protects only data relating to an identifiable individual, whereas "anonymous" data are free to be used by everybody. Usage of many terms related to ...

journal_title：Biopreservation and biobanking

authors： Sariyar M,Schlünder I

更新日期：2016-10-01 00:00:00

abstract：:Modern taxonomy requires the preservation of biospecimens for both morphological and molecular applications. The utility of a previously identified preservative, dimethyldimethylhydantoin hydantoin (Dekafald®), to retain both physical diagnostic traits and the DNA integrity of biological specimens remains unknown. Usi...

journal_title：Biopreservation and biobanking

authors： Bourque DA,Morey KC,Bradley DL,Fost B,Daley JM,Jacobson N,Naaum AM,Hanner RH

更新日期：2020-08-01 00:00:00

abstract：:The Beaumont Health System BioBank was established in 2008, not only to leverage the potential to collect biospecimens for translational research, but to undertake such research in a seamless facility that combined high quality biobanking with state-of-the-art laboratory platforms geared towards biospecimen-based rese...

journal_title：Biopreservation and biobanking

authors： Wilson GD,D'Angelo K,Pruetz BL,Geddes TJ,Larson DM,Akervall J

更新日期：2014-10-01 00:00:00

abstract：:Animal-derived products are widely used in sperm cryopreservation for their cryoprotective properties. These components, however, tend to be replaced because of sanitary risks. STEMALPHA.CRYO3 (Ref. 5617; Stem Alpha, Saint-Genis-l'Argentière, France), called "CRYO3," is a chemically defined preservation medium current...

journal_title：Biopreservation and biobanking

authors： Gavin-Plagne L,Commin L,Bruyère P,Buff S,Joly T

更新日期：2019-01-01 00:00:00

abstract：:In Poland storage of human biological samples takes place at most universities and scientific institutions conducting research projects in the field of biomedicine. The First International Biobanking Conference organized by the Ministry of Science and Higher Education in 2014 shed a light on the situation of Polish bi...

journal_title：Biopreservation and biobanking

authors： Witoń M,Strapagiel D,Gleńska-Olender J,Chróścicka A,Ferdyn K,Skokowski J,Kalinowski L,Pawlikowski J,Marciniak B,Pasterk M,Matera-Witkiewicz A,Kozera Ł

更新日期：2017-06-01 00:00:00

abstract：:The COVID-19 era has brought about a number of novel challenges for the global biobanking community. An array of diverse tools (e.g., standards, best practices, and plans) exists to support quality and fitness-for-purpose in biobank operations. The International Society for Biological and Environmental Repositories (I...

journal_title：Biopreservation and biobanking

authors： Allocca CM,Snapes E,Albert M,Bledsoe MJ,Castelhano MG,De Wilde M,Furuta K,Kozlakidis Z,Martin D,Martins A,McCall SJ,Schacter B

更新日期：2020-12-01 00:00:00

abstract：:Over the past two decades, researchers have increasingly used human biospecimens to evaluate hypotheses related to disease risk, outcomes and treatment. We conducted an analysis of population-science cancer research grants funded by the National Cancer Institute (NCI) to gain a more comprehensive understanding of bios...

journal_title：Biopreservation and biobanking

authors： Carrick DM,Mette E,Hoyle B,Rogers SD,Gillanders EM,Schully SD,Mechanic LE

更新日期：2014-08-01 00:00:00

abstract：:Most university biobanks begin like other university research projects, that is, with an idea conceived by an individual researcher in pursuit of his/her own research interests, publications, funding, and career. Some biobanks, however, come to have scientific value that goes beyond the projects that were initially re...

journal_title：Biopreservation and biobanking

authors： Kongsholm NCH,Christensen ST,Hermann JR,Larsen LA,Minssen T,Pedersen LB,Rajam N,Tommerup N,Tupasela A,Schovsbo J

更新日期：2018-08-01 00:00:00

abstract：:Biobanking at Duke University has existed for decades and has grown over time in silos and based on specialized needs, as is true with most biomedical research centers. These silos developed informatics systems to support their own individual requirements, with no regard for semantic or syntactic interoperability. Duk...

journal_title：Biopreservation and biobanking

authors： Ellis H,Joshi MB,Lynn AJ,Walden A

更新日期：2017-04-01 00:00:00

abstract：:Biobanks and biobanking networks are involved in varying degrees in the collection, processing, storage, and dissemination of biological specimens. This review outlines the approaches that 16 of the largest biobanks and biobanking networks in Europe, North America, Australia, and Asia have taken to collecting and dist...

journal_title：Biopreservation and biobanking

authors： Vaught J,Kelly A,Hewitt R

更新日期：2009-09-01 00:00:00

abstract：:Formalin-fixed paraffin-embedded (FFPE) material presents a readily available resource in the study of various biomarkers. There has been interest in whether the storage period has significant effect on the extracted macromolecules. Thus, in this study, we investigated if the storage period had an effect on the quanti...

journal_title：Biopreservation and biobanking

authors： Kokkat TJ,Patel MS,McGarvey D,LiVolsi VA,Baloch ZW

更新日期：2013-04-01 00:00:00

abstract：:Adipose tissue, which is harvested in large quantities during liposuction, has no blood supply and becomes necrotic within a few hours, if not immediately transplanted. Cryopreservation of adipose tissue allows these samples to be stored and used in diverse fundamental experiments, especially in fat-grafting animal te...

journal_title：Biopreservation and biobanking

authors： Gu L,Sun Y,Wang P,Liu L,Gu J

更新日期：2020-10-09 00:00:00

abstract：:The ability to analyze cryopreserved peripheral blood mononuclear cells (PBMCs) from biobanks for antigen-specific T-cell immunity is necessary to evaluate responses to immune-based therapies. Comprehensive studies have demonstrated that the quality of frozen PBMCs is critical and the maintenance of cell viability and...

journal_title：Biopreservation and biobanking

authors： Germann A,Schulz JC,Kemp-Kamke B,Zimmermann H,von Briesen H

更新日期：2011-09-01 00:00:00

abstract：:Biobanks are now in the spotlight as key enablers supporting preclinical, clinical, and environmental research. Awareness of their value has increased along with the need for these infrastructures to be sustained through business-focused practices. Following our 2017 pilot survey on biobank business planning, we initi...

journal_title：Biopreservation and biobanking

authors： Henderson MK,Goldring K,Simeon-Dubach D

更新日期：2019-06-01 00:00:00

abstract：:Tissue banks represent essential resources and platforms for biomedical research serving basic, translational, and clinical research projects. In this article, we describe 2 models of biobanking and tissue preservation with different approaches and aims. Archive tissue biobanking is described here as a resource of res...

journal_title：Biopreservation and biobanking

authors： Stanta G,Bonin S,Machado I,Llombart-Bosch A

更新日期：2011-06-01 00:00:00

abstract：:l-Carnitine (LC) plays a key role in sperm metabolism, easily providing energy through β-oxidation, which positively affects motility. The objective of this study was to investigate the association between blood plasma and seminal plasma LC levels, as well as the effect of LC as an additive in a skimmed milk-based ext...

journal_title：Biopreservation and biobanking

authors： Nery IHAV,Araújo Silva RAJ,Souza HM,Arruda LCP,Monteiro MM,Seal DCM,Silva GR,Silva TMS,Carneiro GF,Batista AM,Câmara DR,Guerra MMP

更新日期：2020-10-01 00:00:00

abstract：:Cryopreservation, which refers to preservation of cells or tissues at subzero temperatures, inevitably involves the problem of cryoinjury caused by ice crystals. The application of an external electric field during the freezing process has been shown to be a promising approach to produce miniature ice grains and decre...

journal_title：Biopreservation and biobanking

authors： Ma Y,Fan X,Cai J,Tao J

更新日期：2020-10-16 00:00:00

abstract：:Glycerol and dimethyl sulfoxide (DMSO) are widely used cryoprotectants for freezing human cell cultures. During the manufacturing process of ocular stem cell-based autographs, ex vivo cultivated ocular cells are cryopreserved for quality control purposes in accordance with regulatory requirements. The efficiency of th...

journal_title：Biopreservation and biobanking

authors： van Velthoven AJH,Bertolin M,Barbaro V,Sthijns MMJPE,Nuijts RMMA,LaPointe VLS,Dickman MM,Ferrari S

更新日期：2020-11-13 00:00:00

abstract：:We examined whether the introduction of an audit and feedback strategy applied to specimens dissected at the John Hunter Hospital anatomical pathology department improved the number of formalin fixed tumor specimens sampled by anatomical pathology registrars for the Hunter Cancer Biobank. During the audit period (1/7/...

journal_title：Biopreservation and biobanking

authors： King S,Walker M,Neilsen S,O'Brien M

更新日期：2019-01-01 00:00:00

abstract：:It is particularly necessary for biomedical researchers to obtain applicable biosamples accurately and efficiently, especially from a biobank with multiple-disease catalogs. To optimize the retrieval procedure, especially in the early stages of a non-automatic biobank, we developed a procedure that combined the electr...

journal_title：Biopreservation and biobanking

authors： Gao Z,Gu Y,Lv Z,Yu G,Zhou J

更新日期：2015-02-01 00:00:00

abstract：:Bacterial contamination often occurs in human blood DNA samples, possibly due to bacteremia or an inappropriate procedure during sample preparation. This study aimed at analyzing the clinical significance of bacterial DNA contamination in human blood DNA samples and to assess its influence on experimental data. DNA sa...

journal_title：Biopreservation and biobanking

authors： Lee JE,Hong EJ,Shim SM,Kim JW,Bae GR,Cho YS,Jeon JP,Han BG

更新日期：2010-09-01 00:00:00

abstract：:Oocyte vitrification, as a vital step in reproductive medicine, is strongly associated with lower development caused by cryodamaging factors, such as oxidative stress. In this study, we evaluated the antioxidative synergistic effects of Melatonin (Mel) and Resveratrol (RES) coencapsulated by solid lipid nanocarriers (...

journal_title：Biopreservation and biobanking

authors： Aghaz F,Vaisi-Raygani A,Khazaei M,Arkan E

更新日期：2020-12-22 00:00:00

abstract：:RNA is an essential biological material for research in genomics and translational medicine. As such, its storage for biobanking is an important field of study. Traditionally, long-term storage in the cold (generally freezers or liquid nitrogen) is used to maintain high-quality (in terms of quantity and integrity) RNA...

journal_title：Biopreservation and biobanking

authors： Li Q,Wang X,Liu X,Liao Q,Sun J,He X,Yang T,Yin J,Jia J,Li X,Colotte M,Bonnet J

更新日期：2017-12-01 00:00:00

abstract：:The aim of this study was to evaluate the efficiency of different media in the in vitro culture of bovine preantral follicles that were used either fresh or following slow freezing treatment. Frozen and fresh noncultured or cultured ovarian fragments were processed for histological, viability, and cell proliferation a...

journal_title：Biopreservation and biobanking

authors： Castro SV,Carvalho AA,Silva CM,Santos FW,Campello CC,de Figueiredo JR,Rodrigues AP

更新日期：2014-10-01 00:00:00