Abstract:
:Adipose tissue, which is harvested in large quantities during liposuction, has no blood supply and becomes necrotic within a few hours, if not immediately transplanted. Cryopreservation of adipose tissue allows these samples to be stored and used in diverse fundamental experiments, especially in fat-grafting animal tests that could provide a theoretical basis for clinical applications. Traditionally, fetal bovine serum (FBS) has been added as a cryoprotectant (CPA) to maintain the maximum viability of different tissues after freezing and thawing. Calf serum (CS) comes from the same species as FBS but is more economical compared with FBS-containing medium. The optimal concentration of CS in CPA for banking adipose tissue has not been studied. Here, we studied the cell survival rate, cell viability, tissue structural integrity, number of adipose-derived stem cells and blood vessels, and survival after transplantation into nude mice via ultrastructural evaluation of adipose tissue cryopreserved for 6 months in condition A (60% CS, 15% dimethyl sulfoxide [DMSO], 25% Dulbecco's modified Eagle's medium [DMEM]) and condition B (30% CS, 15% DMSO, 55% DMEM). Our results indicate that CS in addition to CPA results in adequate preservation of adipose tissue, especially when a higher concentration of CS (60%) is used in the CPA.
journal_name
Biopreserv Biobankjournal_title
Biopreservation and biobankingauthors
Gu L,Sun Y,Wang P,Liu L,Gu Jdoi
10.1089/bio.2020.0038subject
Has Abstractpub_date
2020-10-09 00:00:00eissn
1947-5535issn
1947-5543pub_type
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journal_title:Biopreservation and biobanking
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journal_title:Biopreservation and biobanking
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journal_title:Biopreservation and biobanking
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journal_title:Biopreservation and biobanking
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journal_title:Biopreservation and biobanking
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
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更新日期:2016-04-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0091
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0099
更新日期:2015-10-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2019.0005
更新日期:2019-06-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0049
更新日期:2014-10-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0076
更新日期:2015-06-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0043
更新日期:2014-02-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0092
更新日期:2016-10-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2020.0066
更新日期:2020-12-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0102
更新日期:2020-12-22 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2011.0024
更新日期:2011-12-01 00:00:00
abstract::Biobank-suisse (BBS) is a collaborative network of biobanks in Switzerland. Since 2005, the network has worked with biobank managers towards a Swiss biobanking platform that harmonizes structures and procedures. The work with biobank managers has shown that long-term, sustainable financing is difficult to obtain. In t...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0057
更新日期:2014-12-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2011.0010
更新日期:2011-06-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0111
更新日期:2017-08-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0072
更新日期:2020-11-11 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2010.0012
更新日期:2010-09-01 00:00:00
abstract::Cryopreservation (CP) is a critical component in enabling on-demand access to biological material (macromolecules, cells, and tissues), yet CP has evolved little over the last several decades. Today's CP processes often yield a suboptimal "product," which has slowed progression in areas such as cell therapy and stem c...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2016.0119
更新日期:2017-04-01 00:00:00
abstract::l-Carnitine (LC) plays a key role in sperm metabolism, easily providing energy through β-oxidation, which positively affects motility. The objective of this study was to investigate the association between blood plasma and seminal plasma LC levels, as well as the effect of LC as an additive in a skimmed milk-based ext...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0025
更新日期:2020-10-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2012.0022
更新日期:2012-08-01 00:00:00
abstract::Systematic cryo-banking of reproductive tissues could enhance reproductive management and ensure sustainability of rare mammalian genotypes. Testicular tissues contain a vast number of germ cells, including at early stages (spermatogonia and spermatocytes), that can potentially develop into viable spermatozoa after gr...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0010
更新日期:2020-06-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0027
更新日期:2013-10-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2018.0067
更新日期:2019-01-01 00:00:00