Abstract:
:Animal-derived products are widely used in sperm cryopreservation for their cryoprotective properties. These components, however, tend to be replaced because of sanitary risks. STEMALPHA.CRYO3 (Ref. 5617; Stem Alpha, Saint-Genis-l'Argentière, France), called "CRYO3," is a chemically defined preservation medium currently used for freezing human tissue and adult stem cells. The aim of this study was to evaluate the effect of a CRYO3-based medium on ram sperm freezing regarding in vitro parameters and in vivo fertility. Semen from nine Charolais rams was collected using an artificial vagina, then split and frozen using two media: a CRYO3-based medium or a control medium containing egg yolk (10%) and milk (45%). Sperm membrane integrity (propidium iodide [PI]/SYBR-14 and calcein AM/ethidium homodimer-1), acrosome integrity (FITC-PNA/PI), and mitochondrial membrane potential (JC-1) were assessed using flow cytometry, while functional membrane integrity was assessed using a hypo-osmotic swelling test and motility parameters, evaluated by computer-assisted sperm analysis. Pregnancy rates, prolificacy, and the average daily weight gain (DWG) of lambs were evaluated after performing 195 laparoscopic inseminations. The control medium showed significantly higher results than CRYO-based medium for all in vitro parameters, except for linearity and straightness (motions parameters). Conversely, field trials showed no significant difference between the control medium and the CRYO3-based medium for pregnancy rates (72.2% and 67.9%, respectively), prolificacy (1.8 and 1.6, respectively), and the DWG (0.34 and 0.35 kg/d, respectively). This preliminary study showed that CRYO3 cannot replace egg yolk and milk in freezing extenders for commercial purposes. However, as laparoscopic inseminations allowed a 67% pregnancy rate, CRYO3-based medium remains an option for international transport or long-term storage of genetic diversity.
journal_name
Biopreserv Biobankjournal_title
Biopreservation and biobankingauthors
Gavin-Plagne L,Commin L,Bruyère P,Buff S,Joly Tdoi
10.1089/bio.2018.0067subject
Has Abstractpub_date
2019-01-01 00:00:00pages
11-17issue
1eissn
1947-5535issn
1947-5543journal_volume
17pub_type
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journal_title:Biopreservation and biobanking
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journal_title:Biopreservation and biobanking
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journal_title:Biopreservation and biobanking
pub_type: 历史文章,杂志文章
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
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更新日期:2016-04-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0046
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章,多中心研究
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更新日期:2012-12-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00
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journal_title:Biopreservation and biobanking
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更新日期:2016-02-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0113
更新日期:2016-10-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2018.0054
更新日期:2018-08-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0010
更新日期:2020-06-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2012.0022
更新日期:2012-08-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2016-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
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更新日期:2017-08-01 00:00:00
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journal_title:Biopreservation and biobanking
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2019.0120
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
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journal_title:Biopreservation and biobanking
pub_type: 杂志文章
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