Increased Cell Survival of Human Primary Conjunctival Stem Cells in Dimethyl Sulfoxide-Based Cryopreservation Media.

Abstract:

:Glycerol and dimethyl sulfoxide (DMSO) are widely used cryoprotectants for freezing human cell cultures. During the manufacturing process of ocular stem cell-based autographs, ex vivo cultivated ocular cells are cryopreserved for quality control purposes in accordance with regulatory requirements. The efficiency of the cryopreservation methods is limited by their effect on cell survival and quality. We compared two cryopreservation reagents, glycerol and DMSO, for their influence on the survival and quality of human primary conjunctival cultures. We found increased cell viability after cryopreservation in DMSO compared to cryopreservation in glycerol. The clonogenic and proliferative capacity was unaffected by the cryopreservation reagents, as shown by the colony forming efficiency and cumulative cell doubling. Importantly, the percentage of p63α- and keratin 19 (K19)-positive cells following cryopreservation in DMSO or glycerol was comparable. Taken together, our results demonstrate that cryopreservation in DMSO improves cell survival compared to cryopreservation in glycerol, with no subsequent effect on cell proliferative-, clonogenic-, or differentiation capacity. Therefore, we advise the use of a 10% DMSO-based cryopreservation medium for the cryopreservation of human primary conjunctival cells, as it will improve the number of cells available for the manufacturing of conjunctival stem cell-based autografts for clinical use.

journal_name

Biopreserv Biobank

authors

van Velthoven AJH,Bertolin M,Barbaro V,Sthijns MMJPE,Nuijts RMMA,LaPointe VLS,Dickman MM,Ferrari S

doi

10.1089/bio.2020.0091

subject

Has Abstract

pub_date

2020-11-13 00:00:00

eissn

1947-5535

issn

1947-5543

pub_type

杂志文章
  • Don't Take It Personal: European Union Legal Aspects of Procuring and Protecting Environmental Exposure Data in Population Biobanks Through the Use of a Geo-Information-Systems Toolkit.

    abstract::Under European Union (EU) law, population-based cohort studies have the right to collect environmental data and to access geospatial data, at street level, on the web, from a host of public sources. As to geospatial information, they should be able to avail themselves of Member States' networks of services for geospat...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2016.0007

    authors: Bovenberg JA,de Hoogh K,Knoppers BM,Hveem K,Hansell AL

    更新日期:2016-06-01 00:00:00

  • Biobanking in the COVID-19 Era and Beyond: Part 2. A Set of Tool Implementation Case Studies.

    abstract::The COVID-19 era has brought about a number of novel challenges for the global biobanking community. An array of diverse tools (e.g., standards, best practices, and plans) exists to support quality and fitness-for-purpose in biobank operations. The International Society for Biological and Environmental Repositories (I...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2020.0083

    authors: Allocca CM,Snapes E,Albert M,Bledsoe MJ,Castelhano MG,De Wilde M,Furuta K,Kozlakidis Z,Martin D,Martins A,McCall SJ,Schacter B

    更新日期:2020-12-01 00:00:00

  • Targeting Future Customers: An Introductory Biobanking Course for Undergraduate Students of Life Sciences.

    abstract:INTRODUCTION:Biobanking is a relatively new concept in the Arab region. Targeting different stakeholders to introduce the concept of biobanking and develop an acceptance of it among them is important for the growth of biobanking in the region. Undergraduate students of life sciences represent an important segment of st...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2016.0111

    authors: Abdelhafiz AS,Fouda MA,El-Jaafary SI,Farghly MI,Salem M,Tammam A,Gabr H

    更新日期:2017-08-01 00:00:00

  • Ethical Legal and Social Issues of Biobanking: Past, Present, and Future.

    abstract::The past 15 years has seen considerable changes in the research environment. These changes include the development of new sophisticated genetic and genomic technologies, a proliferation of databases containing large amount of genotypic and phenotypic data, and wide-spread data sharing among many institutions, national...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章,评审

    doi:10.1089/bio.2017.0030

    authors: Bledsoe MJ

    更新日期:2017-04-01 00:00:00

  • Challenges for the Sustainability of University-Run Biobanks.

    abstract::Most university biobanks begin like other university research projects, that is, with an idea conceived by an individual researcher in pursuit of his/her own research interests, publications, funding, and career. Some biobanks, however, come to have scientific value that goes beyond the projects that were initially re...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2018.0054

    authors: Kongsholm NCH,Christensen ST,Hermann JR,Larsen LA,Minssen T,Pedersen LB,Rajam N,Tommerup N,Tupasela A,Schovsbo J

    更新日期:2018-08-01 00:00:00

  • Enhanced Cryoprotective Effect of Melatonin and Resveratrol by Coencapsulation: Improved In Vitro Development of Vitrified-Warmed Mouse Germinal Vesicle Oocytes.

    abstract::Oocyte vitrification, as a vital step in reproductive medicine, is strongly associated with lower development caused by cryodamaging factors, such as oxidative stress. In this study, we evaluated the antioxidative synergistic effects of Melatonin (Mel) and Resveratrol (RES) coencapsulated by solid lipid nanocarriers (...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2020.0102

    authors: Aghaz F,Vaisi-Raygani A,Khazaei M,Arkan E

    更新日期:2020-12-22 00:00:00

  • The Montpellier Leishmania Collection, from a Laboratory Collection to a Biological Resource Center: A 39-Year-Long Story.

    abstract::We report the development of a laboratory collection of Leishmania that was initiated in 1975 and, after 39 years, has become an international Biological Resource Center (BRC-Leish, Montpellier, France, BioBank No. BB-0033-00052), which includes 6353 strains belonging to 36 Leishmania taxa. This is a retrospective ana...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2015.0101

    authors: Pratlong F,Balard Y,Lami P,Talignani L,Ravel C,Dereure J,Lefebvre M,Serres G,Bastien P,Dedet JP

    更新日期:2016-12-01 00:00:00

  • Consensus-Driven Development of a Terminology for Biobanking, the Duke Experience.

    abstract::Biobanking at Duke University has existed for decades and has grown over time in silos and based on specialized needs, as is true with most biomedical research centers. These silos developed informatics systems to support their own individual requirements, with no regard for semantic or syntactic interoperability. Duk...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2016.0092

    authors: Ellis H,Joshi MB,Lynn AJ,Walden A

    更新日期:2017-04-01 00:00:00

  • Effect of Synergism Between Carboxylated Poly-l-Lysine and Glycerol on Freezability of Nili-Ravi Buffalo (Bubalus bubalis) Semen.

    abstract::Carboxylated poly-l-lysine (CPLL), an ampholytic polymer, has remarkable cryoprotective properties. It was hypothesized that CPLL will reduce/replace glycerol in extender and improve the freezability of buffalo semen. The objective was to evaluate various combinations of CPLL and glycerol in extenders for any synergis...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2019.0120

    authors: Akhter S,Awan MA,Arshad J,Rakha BA,Ansari MS,Iqbal S

    更新日期:2020-10-01 00:00:00

  • Archived formalin-fixed paraffin-embedded (FFPE) blocks: A valuable underexploited resource for extraction of DNA, RNA, and protein.

    abstract::Formalin-fixed paraffin-embedded (FFPE) material presents a readily available resource in the study of various biomarkers. There has been interest in whether the storage period has significant effect on the extracted macromolecules. Thus, in this study, we investigated if the storage period had an effect on the quanti...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2012.0052

    authors: Kokkat TJ,Patel MS,McGarvey D,LiVolsi VA,Baloch ZW

    更新日期:2013-04-01 00:00:00

  • Biobanked Amphibian Samples Confirmed To Species Level Using 16S rRNA DNA Barcodes.

    abstract::The DNA barcoding technique is often used as a tool for validating species identity in biobanks. In the case of amphibians, the mitochondrial DNA (mtDNA) 16S ribosomal RNA (rRNA) gene is reported to fulfill the requirements of a universal DNA barcoding marker. The 16S primers are designed to specifically bind to the 1...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2011.0036

    authors: Maya-Soriano MJ,Holt WV,Lloyd RE

    更新日期:2012-02-01 00:00:00

  • Following nature's lead: generating compounds for stabilizing biomolecules.

    abstract::We describe here a unique approach to identifying small molecules that are useful in stabilizing biomolecules in the dry and liquid states. Using biostability screens aided by in silico docking experiments and synthetic chemistry, libraries of biostability molecules are analyzed for their ability to protect important ...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2012.0022

    authors: Clement O,Whitney S,Muller-Cohn J,Muller R

    更新日期:2012-08-01 00:00:00

  • Factors associated with willingness to participate in biospecimen research among Chinese Americans.

    abstract::A paucity of information exists on the recruitment of Asian Americans for biospecimen research. Although studies show that Chinese Americans are at high risk for hepatitis B virus (HBV) infection, little is known about their willingness to participate in HBV-related biospecimen research and how knowledge, attitudes, a...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2013.0081

    authors: Gao W,Ma GX,Tan Y,Fang C,Weaver J,Jin M,Lai P

    更新日期:2014-04-01 00:00:00

  • Construction of a business model to assure financial sustainability of biobanks.

    abstract::Biobank-suisse (BBS) is a collaborative network of biobanks in Switzerland. Since 2005, the network has worked with biobank managers towards a Swiss biobanking platform that harmonizes structures and procedures. The work with biobank managers has shown that long-term, sustainable financing is difficult to obtain. In t...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2014.0057

    authors: Warth R,Perren A

    更新日期:2014-12-01 00:00:00

  • The challenge of sustaining a hospital-based biobank and core molecular laboratory: the Beaumont experience.

    abstract::The Beaumont Health System BioBank was established in 2008, not only to leverage the potential to collect biospecimens for translational research, but to undertake such research in a seamless facility that combined high quality biobanking with state-of-the-art laboratory platforms geared towards biospecimen-based rese...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2014.0049

    authors: Wilson GD,D'Angelo K,Pruetz BL,Geddes TJ,Larson DM,Akervall J

    更新日期:2014-10-01 00:00:00

  • Improving Ribosomal RNA Integrity in Surgically Resected Human Brain Tumor Biopsies.

    abstract:BACKGROUND:Biopsies extracted from brain cancer patients often display degraded ribosomal RNA, which makes them unusable in transcriptomic experiments. This has not been properly documented in previous works aimed at refining the molecular classification of brain cancer. OBJECTIVE:To determine RNA integrity in a large...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2015.0086

    authors: Castells Domingo X,Ferrer-Font L,Davila M,Candiota AP,Simões RV,Fernández-Coello A,Gabarrós A,Boluda S,Barceló A,Ariño J,Arús C

    更新日期:2016-04-01 00:00:00

  • Metabolomic Quality Assessment of EDTA Plasma and Serum Samples.

    abstract::Handling and processing of blood can significantly alter the molecular composition and consistency of biobank samples and can have a major impact on the identification of biomarkers. It is thus crucial to identify tools to determine the quality of samples to be used in biomarker discovery studies. In this study, a non...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2015.0092

    authors: Malm L,Tybring G,Moritz T,Landin B,Galli J

    更新日期:2016-10-01 00:00:00

  • The use of biospecimens in population-based research: a review of the National Cancer Institute's Division of Cancer Control and Population Sciences grant portfolio.

    abstract::Over the past two decades, researchers have increasingly used human biospecimens to evaluate hypotheses related to disease risk, outcomes and treatment. We conducted an analysis of population-science cancer research grants funded by the National Cancer Institute (NCI) to gain a more comprehensive understanding of bios...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章,评审

    doi:10.1089/bio.2014.0009

    authors: Carrick DM,Mette E,Hoyle B,Rogers SD,Gillanders EM,Schully SD,Mechanic LE

    更新日期:2014-08-01 00:00:00

  • The Informatics Challenges Facing Biobanks: A Perspective from a United Kingdom Biobanking Network.

    abstract::The challenges facing biobanks are changing from simple collections of materials to quality-assured fit-for-purpose clinically annotated samples. As a result, informatics awareness and capabilities of a biobank are now intrinsically related to quality. A biobank may be considered a data repository, in the form of raw ...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2014.0099

    authors: Quinlan PR,Groves M,Jordan LB,Stobart H,Purdie CA,Thompson AM

    更新日期:2015-10-01 00:00:00

  • Short-term stability study of RNA at room temperature.

    abstract::The quality of RNA preserved in different stabilization matrices was investigated after 2 weeks of storage at room temperature. RNA samples in RNAstable (Biomatrica), GenTegra (IntegenX), and RNAshell (Imagene) were compared to RNA stored at -80°C (the current gold standard for RNA preservation) and with liquid or dri...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章,多中心研究

    doi:10.1089/bio.2012.0030

    authors: Mathay C,Yan W,Chuaqui R,Skubitz AP,Jeon JP,Fall N,Betsou F,Barnes M,ISBER Biospecimen Science Working Group.

    更新日期:2012-12-01 00:00:00

  • Models of biobanking and tissue preservation: RNA quality in archival samples in pathology laboratories and "in vivo biobanking" by tumor xenografts in nude mice-two models of quality assurance in pathology.

    abstract::Tissue banks represent essential resources and platforms for biomedical research serving basic, translational, and clinical research projects. In this article, we describe 2 models of biobanking and tissue preservation with different approaches and aims. Archive tissue biobanking is described here as a resource of res...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2011.0010

    authors: Stanta G,Bonin S,Machado I,Llombart-Bosch A

    更新日期:2011-06-01 00:00:00

  • Changing the Culture of Tumor Tissue Biobanking in a Tertiary Referral Hospital Using an Audit and Feedback Strategy.

    abstract::We examined whether the introduction of an audit and feedback strategy applied to specimens dissected at the John Hunter Hospital anatomical pathology department improved the number of formalin fixed tumor specimens sampled by anatomical pathology registrars for the Hunter Cancer Biobank. During the audit period (1/7/...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2018.0100

    authors: King S,Walker M,Neilsen S,O'Brien M

    更新日期:2019-01-01 00:00:00

  • The influence of tissue procurement procedures on RNA integrity, gene expression, and morphology in porcine and human liver tissue.

    abstract::The advent of molecular characterization of tissues has brought an increasing emphasis on the quality of biospecimens, starting with the tissue procurement process. RNA levels are particularly affected by factors in the collection process, but the influence of different pre-analytical factors is not well understood. H...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2014.0076

    authors: Kap M,Sieuwerts AM,Kubista M,Oomen M,Arshad S,Riegman P

    更新日期:2015-06-01 00:00:00

  • Reconsidering Anonymization-Related Concepts and the Term "Identification" Against the Backdrop of the European Legal Framework.

    abstract::Sharing data in biomedical contexts has become increasingly relevant, but privacy concerns set constraints for free sharing of individual-level data. Data protection law protects only data relating to an identifiable individual, whereas "anonymous" data are free to be used by everybody. Usage of many terms related to ...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2015.0100

    authors: Sariyar M,Schlünder I

    更新日期:2016-10-01 00:00:00

  • Cryopreservation and storage effects on cell numbers and DNA damage in human lymphocytes.

    abstract::The comet assay measures DNA damage in individual cells (usually lymphocytes) and is widely used in biomonitoring studies. Lymphocytes are harvested and are usually cryopreserved for batch testing. We investigated cell loss during harvesting, cryopreservation, thawing, and washing of human peripheral lymphocytes and c...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2011.0024

    authors: Ho CK,Choi SW,Siu PM,Benzie IF

    更新日期:2011-12-01 00:00:00

  • Repository Planning, Design, and Engineering: Part I--Infrastructure.

    abstract::This is a two-part review describing the planning, engineering, and design considerations for building a new repository for biological and environmental samples. Part I addresses the physical infrastructure requirements for a repository; Part II will cover equipment and costing. Planning for construction of a new repo...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章,评审

    doi:10.1089/bio.2015.0084

    authors: Baird PM,Gunter EW

    更新日期:2016-04-01 00:00:00

  • Severe Acute Respiratory Syndrome Coronavirus 2 and Coronavirus Disease 2019: A Clinical Overview and Primer.

    abstract::Following its emergence in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused what rapidly became a global pandemic. The precise origin and subsequent path of transmission have not yet been established-but like the other novel coronaviruses that it closely resembles, it appears to have ...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章,评审

    doi:10.1089/bio.2020.0066

    authors: Knight TE

    更新日期:2020-12-01 00:00:00

  • Development of pancreas storage solutions: Initial screening of cytoprotective supplements for β-cell survival and metabolic status after hypothermic storage.

    abstract::Insulin-dependent diabetes mellitus is one of the leading causes of death world-wide. Donor-derived pancreas and Islet of Langerhans transplantation are potential cures; however, postmortem ischemia impacts islet quality. The murine βt3 cell line was employed as a model to study cell viability and proliferation after ...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2012.0023

    authors: Campbell LH,Taylor MJ,Brockbank KG

    更新日期:2013-02-01 00:00:00

  • Biorepository for Pediatric Cancer with Minimal Resources: Meeting the Challenges.

    abstract:BACKGROUND:Conducting high throughput -omics research requires high quality, data-rich biospecimens to unravel factors underlying childhood cancers; this is an extra burden in a limited resources country. For this purpose, Children's Cancer Hospital (CCHE), the largest pediatric cancer hospital worldwide, established a...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2015.0004

    authors: Labib RM,Mostafa MM,Alfaar AS,Yehia D,Alaa M,Elzayat MG,Adel M,Ezzat S,Abo El-Naga S

    更新日期:2016-02-01 00:00:00

  • Human Adipose Tissue Cryopreservation: Impact of Different Calf Serum Concentrations on Tissue Viability.

    abstract::Adipose tissue, which is harvested in large quantities during liposuction, has no blood supply and becomes necrotic within a few hours, if not immediately transplanted. Cryopreservation of adipose tissue allows these samples to be stored and used in diverse fundamental experiments, especially in fat-grafting animal te...

    journal_title:Biopreservation and biobanking

    pub_type: 杂志文章

    doi:10.1089/bio.2020.0038

    authors: Gu L,Sun Y,Wang P,Liu L,Gu J

    更新日期:2020-10-09 00:00:00