Abstract:
:Formalin fixation is known to inactivate most viruses in a vaccine production context, but nothing is published about virus activity in tissues treated with alternative, non-crosslinking fixatives. We used a model assay based on cell culture to test formalin and PAXgene Tissue fixative for their virus-inactivating abilities. MDCK, A549, and MRC-5 cells were infected with Influenza A virus, Adenovirus, and Cytomegalovirus, respectively. When 75% of the cells showed a cytopathic effect (CPE), the cells were harvested and incubated for 15 min, or 1, 3, 6, or 24 hours, with PBS (positive control), 4% formalin, or PAXgene Tissue Fix. The cells were disrupted and the released virus was used to infect fresh MDCK, A549, and MRC-5 cells cultured on cover slips in 24-well plates. The viral cultures were monitored for CPE and by immunocytochemistry (ICC) to record viral replication and infectivity. Inactivation of Adenovirus by formalin occurred after 3 h, while Influenza A virus as well as Cytomegalovirus were inactivated by formalin after 15 min. All three virus strains were inactivated by PAXgene Tissue fixative after 15 min. We conclude that PAXgene Tissue fixative is at least as effective as formalin in inactivating infectivity of Influenza A virus, Adenovirus, and Cytomegalovirus.
journal_name
Biopreserv Biobankjournal_title
Biopreservation and biobankingauthors
Kap M,Arron GI,Loibner M,Hausleitner A,Siaulyte G,Zatloukal K,Murk JL,Riegman Pdoi
10.1089/bio.2013.0010subject
Has Abstractpub_date
2013-08-01 00:00:00pages
229-34issue
4eissn
1947-5535issn
1947-5543journal_volume
11pub_type
杂志文章abstract:: Introduction: Human biological specimen (biospecimen) donation is routinely requested for clinical care and research purposes. Successfully engaging patients and research participants in biospecimen donation depends on what they understand these initiatives entail, includi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0072
更新日期:2020-11-11 00:00:00
abstract::The aim of this study was to evaluate the efficiency of different media in the in vitro culture of bovine preantral follicles that were used either fresh or following slow freezing treatment. Frozen and fresh noncultured or cultured ovarian fragments were processed for histological, viability, and cell proliferation a...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0020
更新日期:2014-10-01 00:00:00
abstract::Over the past two decades, researchers have increasingly used human biospecimens to evaluate hypotheses related to disease risk, outcomes and treatment. We conducted an analysis of population-science cancer research grants funded by the National Cancer Institute (NCI) to gain a more comprehensive understanding of bios...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2014.0009
更新日期:2014-08-01 00:00:00
abstract::Tissues from postmortem transplantation donors are a viable and productive option for genomic research. This entails obtaining authorization from the family decision makers (FDMs) of deceased donors. This study examined best practices for making such requests within the context of the Genotype-Tissue Expression (GTEx)...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2017.0115
更新日期:2018-06-01 00:00:00
abstract::Oocyte vitrification, as a vital step in reproductive medicine, is strongly associated with lower development caused by cryodamaging factors, such as oxidative stress. In this study, we evaluated the antioxidative synergistic effects of Melatonin (Mel) and Resveratrol (RES) coencapsulated by solid lipid nanocarriers (...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0102
更新日期:2020-12-22 00:00:00
abstract::We describe here a unique approach to identifying small molecules that are useful in stabilizing biomolecules in the dry and liquid states. Using biostability screens aided by in silico docking experiments and synthetic chemistry, libraries of biostability molecules are analyzed for their ability to protect important ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2012.0022
更新日期:2012-08-01 00:00:00
abstract::Handling and processing of blood can significantly alter the molecular composition and consistency of biobank samples and can have a major impact on the identification of biomarkers. It is thus crucial to identify tools to determine the quality of samples to be used in biomarker discovery studies. In this study, a non...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0092
更新日期:2016-10-01 00:00:00
abstract::Cryopreservation includes a set of techniques aimed at storing biological samples and preserving their biochemical and functional features without any significant alterations. This study set out to investigate the effects induced by cryopreservation on cultured sheepskin fibroblasts (CSSF) through cryomicroscopy and g...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0100
更新日期:2017-08-01 00:00:00
abstract::The COVID-19 era has brought about a number of novel challenges for the global biobanking community. An array of diverse tools (e.g., standards, best practices, and plans) exists to support quality and fitness-for-purpose in biobank operations. The International Society for Biological and Environmental Repositories (I...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0083
更新日期:2020-12-01 00:00:00
abstract::Adipose tissue, which is harvested in large quantities during liposuction, has no blood supply and becomes necrotic within a few hours, if not immediately transplanted. Cryopreservation of adipose tissue allows these samples to be stored and used in diverse fundamental experiments, especially in fat-grafting animal te...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0038
更新日期:2020-10-09 00:00:00
abstract::Biobank-suisse (BBS) is a collaborative network of biobanks in Switzerland. Since 2005, the network has worked with biobank managers towards a Swiss biobanking platform that harmonizes structures and procedures. The work with biobank managers has shown that long-term, sustainable financing is difficult to obtain. In t...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2014.0057
更新日期:2014-12-01 00:00:00
abstract::We report the development of a laboratory collection of Leishmania that was initiated in 1975 and, after 39 years, has become an international Biological Resource Center (BRC-Leish, Montpellier, France, BioBank No. BB-0033-00052), which includes 6353 strains belonging to 36 Leishmania taxa. This is a retrospective ana...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0101
更新日期:2016-12-01 00:00:00
abstract::Biobanking at Duke University has existed for decades and has grown over time in silos and based on specialized needs, as is true with most biomedical research centers. These silos developed informatics systems to support their own individual requirements, with no regard for semantic or syntactic interoperability. Duk...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0092
更新日期:2017-04-01 00:00:00
abstract::Formalin-fixed paraffin-embedded (FFPE) material presents a readily available resource in the study of various biomarkers. There has been interest in whether the storage period has significant effect on the extracted macromolecules. Thus, in this study, we investigated if the storage period had an effect on the quanti...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2012.0052
更新日期:2013-04-01 00:00:00
abstract::Disease advocacy organizations play an increased role in the development and management of registries and biorepositories. Genetic Alliance developed criteria to assist organizations in selecting vendors and conducted a technical assessment survey of potential vendors. More than half offered customizable solutions, an...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2010.0007
更新日期:2010-06-01 00:00:00
abstract::Biobanks and biobanking networks are involved in varying degrees in the collection, processing, storage, and dissemination of biological specimens. This review outlines the approaches that 16 of the largest biobanks and biobanking networks in Europe, North America, Australia, and Asia have taken to collecting and dist...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2010.0003
更新日期:2009-09-01 00:00:00
abstract::Carboxylated poly-l-lysine (CPLL), an ampholytic polymer, has remarkable cryoprotective properties. It was hypothesized that CPLL will reduce/replace glycerol in extender and improve the freezability of buffalo semen. The objective was to evaluate various combinations of CPLL and glycerol in extenders for any synergis...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2019.0120
更新日期:2020-10-01 00:00:00
abstract::Under European Union (EU) law, population-based cohort studies have the right to collect environmental data and to access geospatial data, at street level, on the web, from a host of public sources. As to geospatial information, they should be able to avail themselves of Member States' networks of services for geospat...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0007
更新日期:2016-06-01 00:00:00
abstract::Human tissue banks are a potential source of cellular material for the nascent cell-based therapy industry; umbilical cord (UC) tissue is increasingly privately banked in such facilities as a source of mesenchymal stem cells for future therapeutic use. However, early handling of UC tissue is relatively uncontrolled du...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2013.0027
更新日期:2013-10-01 00:00:00
abstract::The establishment of a cryopreservation procedure for sheep oocytes will enhance the development of long-term conservation of sheep female genetic resources and the advancement of sheep embryonic biotechnology. However, higher cytoplasmic lipid content and complex subcellular structures affect the relatively higher se...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,评审
doi:10.1089/bio.2017.0074
更新日期:2017-12-01 00:00:00
abstract::Modern taxonomy requires the preservation of biospecimens for both morphological and molecular applications. The utility of a previously identified preservative, dimethyldimethylhydantoin hydantoin (Dekafald®), to retain both physical diagnostic traits and the DNA integrity of biological specimens remains unknown. Usi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2020.0001
更新日期:2020-08-01 00:00:00
abstract::In Poland storage of human biological samples takes place at most universities and scientific institutions conducting research projects in the field of biomedicine. The First International Biobanking Conference organized by the Ministry of Science and Higher Education in 2014 shed a light on the situation of Polish bi...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2016.0091
更新日期:2017-06-01 00:00:00
abstract::RNA is an essential biological material for research in genomics and translational medicine. As such, its storage for biobanking is an important field of study. Traditionally, long-term storage in the cold (generally freezers or liquid nitrogen) is used to maintain high-quality (in terms of quantity and integrity) RNA...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2017.0024
更新日期:2017-12-01 00:00:00
abstract::Most university biobanks begin like other university research projects, that is, with an idea conceived by an individual researcher in pursuit of his/her own research interests, publications, funding, and career. Some biobanks, however, come to have scientific value that goes beyond the projects that were initially re...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2018.0054
更新日期:2018-08-01 00:00:00
abstract::Insulin-dependent diabetes mellitus is one of the leading causes of death world-wide. Donor-derived pancreas and Islet of Langerhans transplantation are potential cures; however, postmortem ischemia impacts islet quality. The murine βt3 cell line was employed as a model to study cell viability and proliferation after ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2012.0023
更新日期:2013-02-01 00:00:00
abstract::The quality of RNA preserved in different stabilization matrices was investigated after 2 weeks of storage at room temperature. RNA samples in RNAstable (Biomatrica), GenTegra (IntegenX), and RNAshell (Imagene) were compared to RNA stored at -80°C (the current gold standard for RNA preservation) and with liquid or dri...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章,多中心研究
doi:10.1089/bio.2012.0030
更新日期:2012-12-01 00:00:00
abstract::The biobanking literature frequently addresses donor and societal issues surrounding biobanking, but the biobanker's perspective is rarely highlighted. While not comprehensive, this article offers an overview of the human aspects of biobanking from the viewpoint of biobank personnel-from biobank formation, through the...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2011.0018
更新日期:2011-09-01 00:00:00
abstract::We examined whether the introduction of an audit and feedback strategy applied to specimens dissected at the John Hunter Hospital anatomical pathology department improved the number of formalin fixed tumor specimens sampled by anatomical pathology registrars for the Hunter Cancer Biobank. During the audit period (1/7/...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2018.0100
更新日期:2019-01-01 00:00:00
abstract::Sharing data in biomedical contexts has become increasingly relevant, but privacy concerns set constraints for free sharing of individual-level data. Data protection law protects only data relating to an identifiable individual, whereas "anonymous" data are free to be used by everybody. Usage of many terms related to ...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0100
更新日期:2016-10-01 00:00:00
abstract::Quality assurance and quality control (QA/QC) procedures are vital to good biorepository management. The National Eye Institute (NEI) core CLIA-certified laboratory of the eyeGENE(®) Network receives blood from individuals with inherited eye conditions and isolates DNA for clinical genetic diagnostic testing and resea...
journal_title:Biopreservation and biobanking
pub_type: 杂志文章
doi:10.1089/bio.2015.0098
更新日期:2016-04-01 00:00:00