Abstract：
BACKGROUND/AIM:MicroRNAs (miRNAs) are closely associated with a number of cellular processes, including cell development, differentiation, proliferation, carcinogenesis, and apoptosis. The aim of the present study was to elucidate the molecular mechanisms underlying the tumor suppressor activity of miRNA-203 (miR-203) in YD-38 human oral cancer cells. MATERIALS AND METHODS:Polymerase chain reaction analysis, MTT assay, DNA fragmentation assay, fluorescence-activated cell-sorting analysis, gene array, immunoblotting, and luciferase assay were carried out in YD-38 cells. RESULTS:miR-203 expression was significantly down-regulated in YD-38 cells compared to expression levels in normal human oral keratinocytes. miR-203 decreased the viability of YD-38 cells in a time- and dose-dependent manner. In addition, over-expression of miR-203 significantly increased not only DNA segmentation, but also the apoptotic population of YD-38 cells. These results indicate that miR-203 overexpression induces apoptosis in YD-38 cells. Target gene array analysis revealed that the expression of the polycomb complex protein gene Bmi-1, a representative oncogene, was significantly down-regulated by miR-203 in YD-38 cells. Moreover, both mRNA and protein levels of Bmi-1 were significantly reduced in YD-38 cells transfected with miR-203. These results indicate that Bmi-1 is a target gene of miR-203. A luciferase reporter assay confirmed that miR-203 suppressed Bmi-1 expression by directly targeting the 3'-untranslated region. CONCLUSION:miR-203 induces apoptosis in YD-38 cells by directly targeting Bmi-1, which suggests its possible application as an anti-cancer therapeutic.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Kim JS,Choi DW,Kim CS,Yu SK,Kim HJ,Go DS,Lee SA,Moon SM,Kim SG,Chun HS,Kim J,Kim JK,Kim DKdoi
10.21873/anticanres.12618subject
Has Abstractpub_date
2018-06-01 00:00:00pages
3477-3485issue
6eissn
0250-7005issn
1791-7530pii
38/6/3477journal_volume
38pub_type
杂志文章abstract：:Two experiments were performed with a chemically induced hepatoma in the rat. In the first, tumors were inoculated subcutaneously into both flanks. After 10 days, when all rats had tumors, treatment was given intravenously on two successive days with 10 mg carmustine/kg body weight/day. All control rats displayed grow...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：AIM:To investigate the relationship between erythrocyte sedimentation rate (ESR) and postoperative survival of patients with colorectal cancer (CRC). MATERIALS AND METHODS:Relationships between clinical characteristics and overall survival (OS) of patients with CRC were investigated using multivariate analysis. Receiv...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13049
更新日期：2018-12-01 00:00:00
abstract：BACKGROUND:Multidrug resistance (MDR) is one of the major obstacles limiting the efficacy of cancer chemotherapy. Through screening a series of natural products, we have previously identified six naturally occurring bisbenzylisoquinoline alkaloids (BBIs) that possess potent activity to reverse P-glycoprotein (gp)-media...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：:The aim of our study was to investigate whether apoptin and e4orf4 pro-apoptotic genes, transferred by means of electroporation, were suitable for gene therapy of tumours. The two genes were chosen for our study because the proteins they encode induce apoptosis in transformed cells only. The apoptin gene was synthesis...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-03-01 00:00:00
abstract：BACKGROUND:It has been demonstrated that 2-Methoxyestradiol (2-ME), one of the estrogen metabolites, induces apoptosis in many different tumor cell lines. In the present study, the effects of 2-ME on human cervical cancer HeLaS3 cells and on normal cervical epithelial cells were evaluated. MATERIALS AND METHODS:Acridi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:Receptors for peanut agglutinin (PNA) were isolated from Lewis lung carcinoma cells (3LL) by detergent solubilization and affinity chromatography on PNA-agarose. The isolated receptors showed heterogeneous yet distinct molecular species when they were analyzed by SDS gel electrophoresis. In vivo inoculation of the iso...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：BACKGROUND/AIM:Extracts of Paeonia suffruticosa are traditionally used in Chinese medicine to increase blood flow. Recently, this extract has been shown to possess anti-tumor and anti-inflammatory properties, though this mechanism remains unknown. In the current work, we prepared extracts of P. suffruticosa and analyze...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11888
更新日期：2017-09-01 00:00:00
abstract：:Mutations in the p53 gene occur frequently in bladder cancers. Better prognostic factors are needed to help select appropriate treatment for patients with TCC stage T1. Paraffin-embedded tumors from 73 patients with TCC stage T1 were processed for two-parameter flow cytometry, measuring both p53 protein and DNA. There...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：BACKGROUND:Breast cancer (BC) is the most common cancer in women, and the hormone receptor status is one of the most important prognostic factors in patients with BC. The aim of this study was to establish whether a relationship exists between the hormone receptor rate and the main classic risk factors in patients with...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：UNLABELLED:Adenomatous polyps are precursor lesions for colorectal carcinoma. The risk of cancer development has been associated with age and size, amount of villous component and high-grade dysplasia of adenomas. The subject-related and adenoma-related risk factors for severely dysplastic lesions were further investig...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
abstract：BACKGROUND:The carcinoembryonic antigen (CEA) is a tumor marker largely utilized for the detection of minimal disease or as a target of immunotherapeutic approaches. In preclinical models CEA has been found to be up-regulated after exposure of cancer cells to 5-fluorouracil (5-FU). In the present study, the clonal dist...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:MDL 103,323, an enclomiphene analog, was tested for binding to the estrogen receptor, inhibition of human tumor xenografts and prevention of carcinogen-induced mammary tumors. MDL 103,323 had 5-6 fold greater affinity for the human estrogen receptor than did either tamoxifen or enclomiphene. Consistent with enhanced b...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:The relationship between serum and cytosolic levels of carcinoembryonic (CEA), squamous-cell carcinoma (SCC) and CA125 antigens was determined in 122 patients with non-small cell lung cancer. A pronounced serum-cytosol gradient and a high degree of dispersion in the distribution of serum and cytosol marker concentrati...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：BACKGROUND:For advanced gastric cancer (AGC) with peritoneal metastasis, decision-making regarding treatment change is often challenging because of the absence of measurable lesions. We attempted to clarify which criterion for treatment change contributes more to longer survival. PATIENTS AND METHODS:We retrospectivel...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：:It was the aim of this study to compare drug-resistant sublines of the murine P388 in relation to resistance markers, the resistant phenotype and immunogenicity. Resistance to drugs either belonging to the MDR type (Doxorubicin, Vincristine, Mitoxantrone) or to the non-MDR type (Methotrexate) was generated in vivo in ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：PURPOSE:We examined the anti-tumor effect of 5-S-GAD, a novel potent inhibitor of protein tyrosine kinases, isolated from the flesh fly in order to investigate the potential use of this compound as an anti-tumor agent. METHODS:In vitro growth inhibition was evaluated using the alamarBlue assay kit. In vivo anti-tumor ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND:Angiogenesis is involved in the development of cancer, promoting tumor growth, invasiveness and metastasis. Vascular endothelial growth factor (VEGF) is a potent angiogenic factor. The present case control study was carried out, to determine whether there is an association between the VEGF -2578C > A polymor...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-07-01 00:00:00
abstract：OBJECTIVE:Breast cancer cells can invade and generate metastasis via either lymphatic or blood vessels. Sialyl Lewis X (SLeX) and Sialyl Lewis a (SLea) are carbohydrate molecules that mediate the adhesion between tumor cells and the endothelium. These antigens are not expressed on normal breast tissue. Overexpression o...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：BACKGROUND:The potential of MTp53 knockout by oligodesoxyribonucleotide phosphothioates (ODNs) to affect proliferation, apoptosis and chemosensitivity in undifferentiated thyroid cancer (UTC) cells with a recessive MTp53 mutation was evaluated. MATERIALS AND METHODS:Transient transfections with ODNs complementary to p...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-03-01 00:00:00
abstract：:Chloroform (CHCl3) is an established rodent carcinogen and a prevalent contaminant of chlorine-disinfected drinking water. Thus in the United States CHCl3, along with other trihalomethanes, is regulated not to exceed 100 ppb in potable water. Recently, several studies have shown that CHCl3 also has anti-cancer propert...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：:During intercellular induction of apoptosis, TGF-beta-treated nontransformed fibroblasts induce apoptosis specifically in transformed fibroblasts. To test whether sensitivity to intercellular induction of apoptosis is causally related to the expression of the transformed phenotype, NRK 536 cells were reversibly transf...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：BACKGROUND/AIM:The European MRI and Rectal Cancer Surgery (EuMaRCS) score was proposed to identify preoperatively difficult laparoscopic total mesorectal excision (L-TME) for locally advanced rectal cancer (LARC). This study aimed to test EuMaRCS's validity. PATIENTS AND METHODS:Data were retrieved from a European mul...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14166
更新日期：2020-04-01 00:00:00
abstract：BACKGROUND/AIM:We have previously reported that doxorubicin (DXR) showed much higher cytotoxicity against human oral squamous cell carcinoma cell lines compared to normal human mesenchymal normal oral cells (gingival fibroblast, periodontal ligament fibroblast, pulp cell), yielding high tumor-specificity. However, we u...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11412
更新日期：2017-03-01 00:00:00
abstract：BACKGROUND/AIM:A prospective study was designed to investigate the effects of anesthesia, particularly that of the one-lung ventilation procedure (OLV), on the expression of hypoxia-inducible factor 1alpha (HIF1alpha) in patients with lung carcinomas and pneumothorax. MATERIALS AND METHODS:The immunohistochemical expr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
abstract：BACKGROUND:The nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway is critical in inflammation, proliferation and carcinogenesis. There exist three main players in this pathway. The inhibitor of NF-κB (IκB), IκB kinase (IκK)- NF-κB essential modulator (NEMO) complex and NF-κB. The IkK-NEMO co...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-05-01 00:00:00
abstract：BACKGROUND:Wilms' tumor 1 (WT1) gene has different functional properties depending on the isoform type. This gene correlates with cell proliferation in various types of cancer. Here, we investigated the expression of WT1 isoforms in breast cancer tissues, and focused on the oncogenic role through estrogen receptor-alph...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
abstract：:Previous studies have demonstrated that berberine decreases N-acetyltransferase (NAT) activity in human leukemia HL-60 cells, however, NAT gene expression has not been investigated. In this study, berberine was selected for testing the inhibition of N-acetylation of 2-aminofluorene (AF) and NAT gene expression in huma...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：BACKGROUND:Warthin-like mucoepidermoid carcinoma is a newly recognized rare entity and could be misdiagnosed as a benign Warthin tumor. We report such a case of a 36-year-old male who presented with a left parotid gland mass. CASE REPORT:Fine-needle aspiration showed features suggestive of Warthin tumor. Following par...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.13461
更新日期：2019-06-01 00:00:00
abstract：:Anaplastic lymphoma kinase (ALK) gene re-arrangements are present in approximately 4% of patients with non-small cell lung cancer (NSCLC), mostly in non-smokers with adenocarcinoma. V-KI-RAS2 Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations are more common in smokers. These molecular lesions were usually de...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：BACKGROUND:The rat model of breast cancer induced by the administration of N-methyl-nitrosourea (NMU) constitutes a useful tool for dissecting the initiation, promotion and progression process of carcinogenesis. Angiogenesis, the recruitment of new blood vessels, is an essential component of the metastatic pathway. Tum...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
