Abstract：
:The aim of our study was to investigate whether apoptin and e4orf4 pro-apoptotic genes, transferred by means of electroporation, were suitable for gene therapy of tumours. The two genes were chosen for our study because the proteins they encode induce apoptosis in transformed cells only. The apoptin gene was synthesised based on a published nucleotide sequence. MTT and TUNEL tests confirmed that both the synthesised apoptin gene and the e4orf4 gene indeed induced apoptosis in COS-7, Renca and B16(F10) cell lines. Therapeutic DNA was then administered via electroporation directly into murine B16(F10) tumours. Distinct tumour growth inhibition was seen only during the treatment. The cessation of therapy caused tumour re-growth. Obviously, the efficiency of gene transfer using electroporation is low and did not induce a permanent therapeutic effect.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Mitrus I,Missol-Kolka E,Plucienniczak A,Szala Ssubject
Has Abstractpub_date
2005-03-01 00:00:00pages
1087-90issue
2Aeissn
0250-7005issn
1791-7530journal_volume
25pub_type
杂志文章abstract：BACKGROUND/AIM:The associations between emotional personality, proximity and authenticity in patient-physician communication during breast cancer (BC) consultations are rarely considered together in a prospective study. We, therefore, investigated emotional personality/proximity versus authenticity in patient-physician...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
abstract：PURPOSE:The present project studied the implications of using the irregular surface compensator (ISC) technique in comparison to three-dimensional conformal radiation therapy (3D-CRT) for breast cancer treatment. ISC is an electronic compensation algorithm that modulates the fluence across the radiation fields to compe...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：BACKGROUND:The concentration of Des-gamma-carboxy-prothrombin (DCP) or PIVKA-II has been described to be increased in patients with hepatocellular cancer, along with its elevation in vitamin K deficient states by warfarin or dicoumarol treatment. The aim of the study was to investigate its clinical value in HCC in comp...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：BACKGROUND:Measurement of cell survival as performed by the differential staining cytotoxicity (DiSC) assay allows for a comprehensive prediction of individual chemotherapy response. This assay has not reached routine clinical application because of its several limitations. In order to improve the DiSC assay, we develo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-06-01 00:00:00
abstract：:The immunomodulatory and antimetastatic/antitumor activity of thymic humoral factor-gamma 2 (THF-gamma 2) was evaluated in BALB/c-mice. Daily subcutaneous applications (7 consecutive days, 20, 200 ng of THF-gamma 2 per injection/mouse) upregulated counts of thymocytes and peripheral blood cells in tumor bearing mice. ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：BACKGROUND/AIM:The purpose of this study was to consider appropriate application of liquid and re-biopsy through analysis of current status in practice. PATIENTS AND METHODS:We performed a retrospective analysis of 22 patients with epidermal growth factor receptor (EGFR) mutation-positive non-small cell lung cancer wh...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12628
更新日期：2018-06-01 00:00:00
abstract：:Important strategies against cancer are based on the understanding of the mechanisms of tumor progression. To elucidate alterations regarding tumor progression, we have performed proteomic differential display analysis for the expression of intracellular proteins in the regressive murine fibrosarcoma cell clone QR-32 ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：:The present study was undertaken to investigate cell death (particularly apoptosis) induced by etoposide, radiation, and both, and to examine p53 protein expression in relation to cell death. Nude mice transplanted with a human tumor (ependymoblastoma) were treated with etoposide (5-40 mg/kg) or 1-2 Gy X-ray irradiati...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Human urothelial cell lines of non-malignant and malignant origin were classified into three grades of transformation, i.e. TGr I, TGr II, and TGr III, which differ from primary and early cultures of normal bladder mucosa (TGr 0) by growth in the absence of fibroblasts, prolonged survival, increasing chromosomal abnor...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:The response of the blood-forming and supportive stromal compartments of the marrow to a localized, fractionated course of radiotherapy [FxRT; 2.0 Gy (q24 h x 5)-->74 Gy] was examined in the C57Bl/6 mouse using the hematopoietic progenitor and the cobblestone-forming cell (CAFC) assays as end-points for the blood-form...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：BACKGROUND:TAOS1 (tumor amplified and overexpressed sequence 1) was recently cloned and found to be possibly important in driving the amplification of 11q13 in oral squamous cell carcinoma (SCC). MATERIALS AND METHODS:Quantitative RT-PCR was performed to determine the possible relationship between TAOS1 gene expressio...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-05-01 00:00:00
abstract：:The inhibitory activity of lignin against nitrosoguanidine (MNNG)- and acridine orange (AO)- induced mutagenesis was examined using two microbial systems: green unicellular flagellate Euglena gracilis and Salmonella typhimurium TA100 and TA97. To verify the hypothesis that the above mentioned mutagens may generate som...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:The effect of PSK, a protein-bound polysaccharide and an immunomodulator, on lymphocytes was examined in vitro for 36 patients with gastric cancer and 26 with colorectal cancer. Cultured lymphocytes with PSK at 100 micrograms/ml increased the level of DNA synthesis, as determined by the 3H-thymidine uptake, from 0.9 t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：AIM:To evaluate the role of radiotherapy alone or combined with chemotherapy in the treatment of recurrent vulvar cancer, emphasising the prognostic factors and outcomes. MATERIALS AND METHODS:Twenty women with loco-regional recurrence of vulvar carcinoma were retrospectively reviewed. Eleven patients were managed wit...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:Menogaril is an antitumor agent active after oral administration, and unlike other anthracyclines, extravasation cannot occur. When the IC90 values of menogaril were plotted versus exposure time on a double-logarithmic scale, the regression lines had slopes between -0.64 and -0.80. These results showed that the mode o...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：BACKGROUND/AIM:3,4',7-O-trimethylquercetin (34'7TMQ), a derivative of quercetin, inhibited ovarian cancer cell migration and invasion without affecting proliferation. In this study, the apoptotic effect of 34'7TMQ on three cancer cell lines (CRL-1978, CRL-11731, SK-OV-3) was evaluated. MATERIALS AND METHODS:Expression...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12835
更新日期：2018-09-01 00:00:00
abstract：:AD 198, a novel lipophilic N-alkyl derivative of adriamycin (ADR) and a potential anticancer agent for preclinical development, was studied for its effects on the activities of DNA and RNA polymerases in vitro and its ability to bind DNA. AD 198, which contains a benzyl substituent on the glycosidic amine, was found t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：BACKGROUND/AIM:Extracts of Paeonia suffruticosa are traditionally used in Chinese medicine to increase blood flow. Recently, this extract has been shown to possess anti-tumor and anti-inflammatory properties, though this mechanism remains unknown. In the current work, we prepared extracts of P. suffruticosa and analyze...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11888
更新日期：2017-09-01 00:00:00
abstract：AIM:The purpose of this prospective study was to evaluate single-frequency whole-body bioelectrical impedance analysis (BIA) as a predictor for the onset of edema of the upper limb in patients undergoing treatment for breast cancer. MATERIALS AND METHODS:Whole-body BIA was performed before surgery, as well as at two d...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：BACKGROUND:Pulmonary complications (PCs) after esophagectomy for patients with esophageal cancer have been correlated with prolonged hospital stays and in-hospital mortality. Previous studies have shown that minimally-invasive esophagectomy (MIE) is associated with a lower rate of PCs compared to conventional open surg...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-05-01 00:00:00
abstract：:The Feulgen-DNA content of squamous carcinoma cell nuclei from the human esophagus was assessed in punch biopsies from 47 untreated patients. Forty-four of the 47 biopsies (93.6%) demonstrated aneuploid cell populations, and the remaining 3 (6.4%) were non-diploid. Previous studies have demonstrated that in esophageal...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：:Four sorts of tumor proliferation-related markers were studied in 50 papillary carcinomas of the thyroid, and the results were correlated with the histologic subtypes of papillary thyroid carcinoma, which have been shown to be associated with the prognosis of the disease. The mitotic count analyzed by light microscopy...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:The design of treatment for patients with squamous cell carcinoma of the head and neck (SCCHN) is based on prognostic factors, today essentially performance status and TNM status. It becomes increasingly important to optimise treatment and find the appropriate level of treatment and resource consumption for each patie...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1996-07-01 00:00:00
abstract：:The most active fraction (Fr. VI) of antitumor and antiviral pine cone extract was subjected to chemical structure analysis. IR, UV, and ESR spectroscopies, in addition to chromatographic analysis on cellulose-TLC plates, reveal that the major component of Fr. VI includes structures belonging to the lignin family. A c...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-11-01 00:00:00
abstract：BACKGROUND/AIM:The effects of inorganic and organic arsenicals on proliferation, cell-cycle distribution, and apoptosis of all-transretinoic acid (ATRA)-resistant human promyelocytic leukemia HL-60 (HL-60-R2) cells were herein investigated. MATERIALS AND METHODS:Cell proliferation was examined by 3-(4,5-dimethylthiazo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-11-01 00:00:00
abstract：:The poor prognosis of advanced gastric carcinoma requires new therapeutic approaches. Among these, the specific activation of resting lymphocytes by bispecific antibodies may be promising. Here we describe the generation and function of a bispecific monoclonal antibody (bi-mab) with specificity for CD3 and for the tum...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：BACKGROUND:Hurthle cell neoplasms may occur as a benign adenoma or carcinoma; the latter displays comparatively aggressive clinical behaviour. Fine-needle aspiration cytology (FNAC) represents a reliable tool for screening Hurthle cell lesions before surgery. Nevertheless, the cytological interpretation of these lesion...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-07-01 00:00:00
abstract：:Most current anticancer drugs have shown excellent therapeutic effects on human oral squamous cell carcinoma (OSCC), but they also produce potent cytotoxicity in normal oral keratinocytes. This review article summarizes our extensive research of tumor specificity and keratinocyte toxicity of nine groups of compounds s...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.12038
更新日期：2017-11-01 00:00:00
abstract：:Autophagy is an evolutionarily-conserved catabolic process which furthers cell survival, especially in times of nutritional stress. Whilst being by itself a pro-survival mechanism, it has many areas of overlap with apoptosis. Autophagic cell death is recognised as a subset of programmed cell death, with features more ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND/AIM:Polyamines are important for the growth of eukaryotic cells. At high levels, they promote proliferation, invasion and migration of tumour cells. Polyamine metabolism is an important new target for anticancer therapy. Some polyamine analogues can have an inhibitory effect on tumour cells. The aim of this ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14085
更新日期：2020-03-01 00:00:00
