Abstract：
:Podocyte effacement and the reformation of foot processes and slit diaphragms can be induced within minutes experimentally. Therefore, it seems likely that the slit diaphragm proteins underlie orchestrated recycling mechanisms under the control of posttranslational modifiers. One of these modifiers, SUMO (small ubiquitin-like modifier), is an ubiquitin-like protein with a 20% corresponding identity to ubiquitin. Modification by SUMOs to proteins on lysine residues can block the ubiquitination of the same site leading to the stabilization of the target protein. Here we found in vitro and in vivo that nephrin is a substrate modified by SUMO proteins thereby increasing its steady-state level and expression at the plasma membrane. A conversion of lysines to arginines at positions 1114 and 1224 of the intracellular tail of murine nephrin led to decreased stability of nephrin, decreased expression at the plasma membrane, and decreased PI3K/AKT signaling. Furthermore, treatment of podocytes with the SUMOylation inhibitor ginkgolic acid led to reduced membrane expression of nephrin. Similarly, the conversion of lysine to arginine at position 1100 of human nephrin caused decreased stability and expression at the plasma membrane. As SUMOylation is a reversible process, our results suggest that SUMOylation participates in the tight orchestration of nephrin turnover at the slit diaphragm.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Tossidou I,Himmelseher E,Teng B,Haller H,Schiffer Mdoi
10.1038/ki.2014.198subject
Has Abstractpub_date
2014-12-01 00:00:00pages
1161-73issue
6eissn
0085-2538issn
1523-1755pii
S0085-2538(15)30450-6journal_volume
86pub_type
杂志文章abstract：:To assess in vitro erythropoietic cultures as a tool to discriminate between patients whose anemia improves with and those whose anemia fails to improve with androgens, bone marrows of 24 anemic patients receiving maintenance hemodialysis in hormone-depleted plasma clots were cultured. While erythroid colony prolifera...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1982.186
更新日期：1982-10-01 00:00:00
abstract：BACKGROUND:Decay accelerating factor (DAF), a product of mesangial cells in vitro, is expressed on the surface of cells and is a candidate for the focal suppression of complement activation. It is not clear at present whether the levels of expression of DAF and intrarenal C3 synthesis correlate with the level of tissue...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1998.00961.x
更新日期：1998-07-01 00:00:00
abstract：:A morphometric technique is used to estimate the absolute and relative surface areas of the brush border microvilli and cell walls bordering lateral intercellular spaces. In isolated, perfused proximal tubule from rabbit, the luminal and lateral surfaces are equal in area. For proximal convoluted tubules (PCT) each su...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1975.125
更新日期：1975-12-01 00:00:00
abstract：:Australia's Indigenous people have high rates of chronic kidney disease and kidney failure. To define renal disease among these people, we reviewed 643 renal biopsies on Indigenous people across Australia, and compared them with 249 biopsies of non-Indigenous patients. The intent was to reach a consensus on pathologic...
journal_title：Kidney international
pub_type： 杂志文章,多中心研究
doi：10.1038/ki.2012.307
更新日期：2012-12-01 00:00:00
abstract：:Mutations in the MYH9 gene, coding for the non-muscle myosin heavy chain IIA (NMHC-IIA), are responsible for syndromes characterized by macrothrombocytopenia associated with deafness, cataracts, and severe glomerular disease. Electron microscopy of renal biopsies from these patients found glomerular abnormalities char...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2011.256
更新日期：2011-11-01 00:00:00
abstract：:Macrophages are inflammatory cells with important roles in the propagation of renal injury. More recently they have also been shown to be important in the resolution of inflammation. Wang et al. show that macrophages can be modulated ex vivo by cytokine stimulation, localize to renal tissue, and slow progression of ex...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/sj.ki.5002332
更新日期：2007-08-01 00:00:00
abstract：BACKGROUND:Optimal nutrient intake is important in the maintenance of a positive nitrogen balance in hemodialysis (HD) patients. The objectives of this study were (1) to assess the influence of two levels of protein intakes on nitrogen balance in stable adult HD patients, and (2) to identify a minimum level of protein ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2000.00171.x
更新日期：2000-07-01 00:00:00
abstract：:Starting from observations in intact cells and extending to studies in native membranes and solubilized membrane proteins, a significant body of evidence has been accumulated to indicate that some of the short-term regulatory influences on the Na+-H+ exchanger in the apical membrane of the proximal convoluted tubule a...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.1989.226
更新日期：1989-10-01 00:00:00
abstract：:Autosomal dominant polycystic kidney disease (ADPKD) is a renal hereditary disorder associated with increased cardiovascular mortality, due to mutations in polycystin-1 and polycystin-2 genes. Endothelial polycystin-deficient cells have an altered mechanosensitivity to fluid shear stress and subsequent deficit in calc...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2014.241
更新日期：2015-02-01 00:00:00
abstract：BACKGROUND:Tumor necrosis factor (TNF)-alpha rapidly primes neutrophils (PMN) for an anti-neutrophil cytoplasmic antibody (ANCA)-induced respiratory burst and is thus proinflammatory. TNF-alpha also progressively accelerates apoptosis. We investigated the effect of TNF-alpha-mediated apoptosis on ANCA antigen expressio...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2002.00161.x
更新日期：2002-02-01 00:00:00
abstract：:Many features of chronic kidney disease may be reversed, but it is unclear whether advanced lesions, such as adhesions of sclerotic glomerular tufts to Bowman's capsule (synechiae), can resolve during treatment. We previously showed, using a renal ablation model, that the renoprotective effect of the AT-1 receptor blo...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.528
更新日期：2009-01-01 00:00:00
abstract：:Coronary artery stenosis induces renal inflammation and kidney injury in pigs even in the absence of myocardial infarction or clinically significant heart failure. This effect is aggravated by experimentally induced renovascular hypertension. Interestingly, oxidative stress originating from the ischemic myocardium was...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2014.431
更新日期：2015-04-01 00:00:00
abstract：:Diabetes mellitus is associated with natriuresis, whereas estrogen has been shown to be renoprotective in diabetic nephropathy and may independently regulate renal sodium reabsorption. The aim of this study was to determine the effects of 17-beta estradiol (E(2)) replacement to diabetic, ovariectomized (OVX) female ra...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5000140
更新日期：2006-02-01 00:00:00
abstract：:In rats undergoing renal mass reduction (RMR) oral supplementation with the nitric oxide (NO) precursor L-arginine increases glomerular filtration rate and ameliorates signs of glomerular injury, suggesting that chronic renal failure in the rats is a condition of low NO formation in the kidney. On the contrary, data a...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1997.317
更新日期：1997-07-01 00:00:00
abstract：:We evaluated short- and long-term effects of high-dose recombinant human erythropoietin (rHuEPO) in kidney transplantation in a prospective double-blind, placebo-controlled study. Patients with chronic kidney disease following receipt of a deceased donor kidney allograft were randomized to 3 doses of 40,000 units rHuE...
journal_title：Kidney international
pub_type： 杂志文章,随机对照试验
doi：10.1038/ki.2011.349
更新日期：2012-02-01 00:00:00
abstract：:Cultures of human proximal tubule cells were exposed to elevated concentrations of glucose and dome formation was assessed over a 22 day period of growth. Cultures grown on 5.5 mM glucose formed five domes per microscopic field while those exposed to elevated glucose concentrations (11.0 mM to 27.5 mM) formed only two...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1989.5
更新日期：1989-01-01 00:00:00
abstract：:The formation of the non-sulphated glycosaminoglycan hyaluronan by cells of the renal glomerulus in diabetes may contribute to altered matrix composition. We describe an increased production of hyaluronan from mesangial cell-enriched glomerular cores from diabetic animals, and further show that increased hyaluronan pr...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.284
更新日期：1996-07-01 00:00:00
abstract：:ESKD patients have a large burden of disease, with high rates of readmission to hospital compared with the general population. A readmission after an acute index hospital discharge is either planned or unplanned. A proportion of unplanned readmissions are potentially avoidable, and could have been prevented with optim...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.2015.307
更新日期：2015-12-01 00:00:00
abstract：BACKGROUND:Hypercholesterolemia may affect both endothelial function and arterial distensibility (DC). Renal transplant recipients (NTX) exhibit advanced structural and functional alterations of arterial vessel walls. The aim of this double-blind, randomized trial was to evaluate the effects of fluvastatin (FLU) on bra...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1046/j.1523-1755.2001.0590041473.x
更新日期：2001-04-01 00:00:00
abstract：:Renal blood oxygen level-dependent magnetic resonance imaging (BOLD-MRI) is a noninvasive fast technique to characterize renal function. Here we evaluated the impact of renal function on the relaxation rate (R2(*)) in the cortex and medulla to provide baseline data for further use of renal BOLD-MRI. This parameter was...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2011.455
更新日期：2012-04-01 00:00:00
abstract：:To define the pattern of recovery from postischemic acute renal failure (ARF), we performed clearance and micropuncture studies at intervals of 1, 2, 4, and 8 weeks following 60 min of complete unilateral renal artery occlusion in the rat. At 1 week, the inulin clearance (CIn) of the postischemic kidney was less than ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1979.112
更新日期：1979-08-01 00:00:00
abstract：BACKGROUND:Altered mineral metabolism contributes to bone disease, cardiovascular disease, and other clinical problems in patients with end-stage renal disease. METHODS:This study describes the recent status, significant predictors, and potential consequences of abnormal mineral metabolism in representative groups of ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2005.00185.x
更新日期：2005-03-01 00:00:00
abstract：:Mitochondrial diseases represent a significant clinical challenge. Substantial efforts have been devoted to identifying therapeutic strategies for mitochondrial disorders, but effective interventions have remained elusive. Recently, we reported attenuation of disease in a mouse model of the human mitochondrial disease...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2018.08.038
更新日期：2019-02-01 00:00:00
abstract：:A major contributor to the development and progression of ischemia-reperfusion (IR)-induced acute renal failure (ARF) is the loss of functioning tubular epithelial cells by means of various cell deletion or death processes. Although the term "acute tubular necrosis" is still used to describe the pathology of ARF, this...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.00701.x
更新日期：1999-10-01 00:00:00
abstract：BACKGROUND:Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one) is a potent scavenger of free radicals and has the antioxidant ability to inhibit lipid peroxidation. Its protective effect on brain ischemia has been shown. This study aimed to elucidate its possible therapeutic effects on renal oxidative stress in a rat ische...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2004.00567.x
更新日期：2004-05-01 00:00:00
abstract：:Urinary peptidomics focuses on endogenous urinary peptide content. Many studies now show the usefulness of this approach for the discovery and validation of biomarkers in kidney diseases that are as varied as chronic kidney disease, acute kidney injury, congenital anomalies of the kidney and the urinary tract, and pol...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1016/j.kint.2015.10.010
更新日期：2016-03-01 00:00:00
abstract：:Multiple microRNAs (miRs) are implicated in muscle cell differentiation and muscle mass regulation. Pharmacological agents targeting miR-486 and other miRs, involved in muscle mass regulation, could potentially be developed into therapeutic agents for muscle wasting. Muscle wasting is prevalent among patients with chr...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2012.150
更新日期：2012-08-01 00:00:00
abstract：:The aging process in humans and in the rat is associated with an impairment in renal tubular reabsorption of Pi and renal tubular adaptation to a low Pi diet. The purposes of the present study were to determine whether changes in the abundance of type II Na-Pi contransporter (NaPi-2) protein and/or mRNA play a role in...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.385
更新日期：1996-09-01 00:00:00
abstract：:Early graft loss (EGL) is a feared outcome of kidney transplantation. Consequently, kidneys with an anticipated risk of EGL are declined for transplantation. In the most favorable scenario, with optimal use of available donor kidneys, the donor pool size is balanced by the risk of EGL, with a tradeoff dictated by the ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2020.01.043
更新日期：2020-06-01 00:00:00
abstract：:We report the first case of a mitochondrial DNA (mtDNA) deletion diagnosed by renal biopsy. An eight-year-old girl with megaloblastic anemia and severe growth retardation developed progressive renal insufficiency accompanied by partial Fanconi syndrome. Histologic examination of the renal biopsy disclosed nonspecific ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1994.181
更新日期：1994-05-01 00:00:00
