Abstract：
:Cultures of human proximal tubule cells were exposed to elevated concentrations of glucose and dome formation was assessed over a 22 day period of growth. Cultures grown on 5.5 mM glucose formed five domes per microscopic field while those exposed to elevated glucose concentrations (11.0 mM to 27.5 mM) formed only two to three domes per field. The areas of the domes formed by the cells grown on elevated glucose concentrations were reduced as compared to those formed on 5.5 mM glucose. An analysis of the electrical properties of cells grown on elevated glucose concentrations by Ussing chamber technique disclosed a marked reduction in potential difference, short circuit current, and resistance compared to cells grown on 5.5 mM glucose. Routine ultrastructural analysis disclosed that cells grown on elevated glucose concentrations appeared to have fewer tight junction complexes. Further examination utilizing freeze fracture methodology demonstrated that cells grown on elevated glucose concentrations averaged two to three sealing strands per junction as compared to an average of five sealing strands for cells grown on 5.5 mM glucose. The cells grown on elevated glucose concentrations were also noted to possess a greater number of gap junctions. These results demonstrate that elevated glucose concentrations can alter the paracellular route, and possibly the transcellular route, of transport regulation in cultured human proximal tubule cells.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Hazen-Martin DJ,Sens MA,Detrisac CJ,Blackburn JG,Sens DAdoi
10.1038/ki.1989.5subject
Has Abstractpub_date
1989-01-01 00:00:00pages
31-9issue
1eissn
0085-2538issn
1523-1755pii
S0085-2538(15)34461-6journal_volume
35pub_type
杂志文章abstract：BACKGROUND:This multicenter, open-label, dose-titration study assessed the safety and efficacy of RenaGel(R), a nonabsorbed calcium- and aluminum-free phosphate binder, in lowering serum phosphorus. Secondary outcomes were its effects on serum intact parathyroid hormone (iPTH) and serum lipids. METHODS:Phosphate binde...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1046/j.1523-1755.1999.00240.x
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND:Low dialysate calcium (LdCa) concentration is used to prevent or treat hemodialysis (HD)-induced hypercalcemia, but its use has been complicated by intradialytic hypotension in some patients. Our goal was to explore the possibility that dialysis calcium profiling (dCaP) can ameliorate intradialytic hypotensi...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1046/j.1523-1755.2002.00100.x
更新日期：2002-01-01 00:00:00
abstract：:Most epidemiologic data, thus far, have focused on short-term outcomes of acute kidney injury (AKI). Lo et al. correlate AKI with long-term outcomes. The concept of 'uremic memory' sheds light on the importance of AKI and its permanent imprint. The focus of research should be on prevention of an episode of AKI, when p...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2009.314
更新日期：2009-10-01 00:00:00
abstract：:Acute kidney injury (AKI) activates pathways of cell death and cell proliferation. Although seemingly discrete and unrelated mechanisms, these pathways can now be shown to be connected and even to be controlled by similar pathways. The dependence of the severity of renal-cell injury on cell cycle pathways can be used ...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.2009.224
更新日期：2009-09-01 00:00:00
abstract：:The involvement of sodium/potassium-ATPase in regulating parathyroid hormone (PTH) secretion is inferred from in vitro studies. Recently, the α-klotho-dependent rapid recruitment of this ATPase to the parathyroid cell plasma membrane in response to low extracellular calcium ion was suggested to be linked to increased ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2010.501
更新日期：2011-04-01 00:00:00
abstract：:Iron is critical for cellular, organismal, and possibly universal existence. Use of iron complexes to treat human diseases is ancient and is described in detail in Ayurveda/Siddha systems of medicine. Old aphorisms from Siddha medicine ("Alavukku Minjinal Amirdhamum Nanjagum," an elixir turns poisonous when taken in e...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2016.02.035
更新日期：2016-07-01 00:00:00
abstract：:A newly described glomerulotubular feedback loop may explain the relationship between glomerular damage, epitope spreading, tubulointerstitial nephritis, proteinuria as a progression factor, and the importance of the local milieu in kidney damage. It also opens the horizons for exciting innovative approaches to therap...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.2009.489
更新日期：2010-03-01 00:00:00
abstract：:Measures related to red cell oxygen transport were evaluated in 17 hemodialysis patients receiving androgen therapy, 15 untreated hemodialysis patients and 15 normal subjects. Hemoglobin levels were higher in androgen-treated patients than in the untreated population and were directly related to the reticulocyte index...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1987.15
更新日期：1987-01-01 00:00:00
abstract：:Early graft loss (EGL) is a feared outcome of kidney transplantation. Consequently, kidneys with an anticipated risk of EGL are declined for transplantation. In the most favorable scenario, with optimal use of available donor kidneys, the donor pool size is balanced by the risk of EGL, with a tradeoff dictated by the ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2020.01.043
更新日期：2020-06-01 00:00:00
abstract：BACKGROUND:Cux-1 is a murine homeobox gene that is highly expressed in the nephrogenic zone of the developing kidney. Transgenic mice ectopically expressing Cux-1 develop renal hyperplasia associated with down-regulation of the cyclin kinase inhibitor p27. Because the reduction of p27 has been associated with mesangial...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00889.x
更新日期：2003-04-01 00:00:00
abstract：:Urinary exosomes have received considerable attention as a potential biomarker source for the diagnosis of renal diseases. Notwithstanding, their use in protein biomarker research is hampered by the lack of efficient methods for vesicle isolation, lysis, and protein quantification. Here we report an improved ultracent...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2012.25
更新日期：2012-06-01 00:00:00
abstract：:Renal toxicity, possibly due to vasoconstriction and vascular injury, is the most relevant side-effect of chronic cyclosporine (CsA) therapy given to prevent graft rejection. In kidney transplant recipients each oral dose of CsA is invariably followed by a transient reduction in renal plasma flow (RPF) and glomerular ...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1038/ki.1993.101
更新日期：1993-03-01 00:00:00
abstract：:Abnormalities of bone mineral metabolism and vascular calcification are prevalent in patients with kidney failure. Clinical management is based on biochemical targets, in particular parathyroid hormone (PTH) concentrations, but this has many limitations including high biological variation. A possible alternative is bo...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2012.77
更新日期：2012-07-01 00:00:00
abstract：BACKGROUND:In a prior study the 21-aminosteroid (lazaroid) U74389F provided in vivo protection from oxidative stress when used as a preventive therapy in ischemia-reperfusion injury in the kidney. As the cell membrane is the principal site for lipoperoxidation, in the current study the very lipophilic 2-methylaminochro...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1998.00072.x
更新日期：1998-09-01 00:00:00
abstract：:Urinary exosomes containing apical membrane and intracellular fluid are normally secreted into the urine from all nephron segments, and may carry protein markers of renal dysfunction and structural injury. We studied methods for collection, storage, and preservation of urinary exosomal proteins. We collected urine fro...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5000273
更新日期：2006-04-01 00:00:00
abstract：:The intracellular distribution of carbonic anhydrase was studied in nine human donor kidneys by the histochemical method of Hansson and by an immunofluorescence technique using antisera specific against the cytoplasmic isoenzymes HCA B and HCA C. Only HCA C was found in the renal tubules. Convoluted proximal tubules s...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1984.106
更新日期：1984-06-01 00:00:00
abstract：:Management of idiopathic glomerular disease associated with nephrotic syndrome (INS) remains controversial and one of the most complex areas relates to utilization of the drug cyclosporin. This is despite its demonstrated effectiveness in several histologic types of the INS in randomized controlled trials. Cyclosporin...
journal_title：Kidney international
pub_type： 共识发展会议,杂志文章
doi：10.1038/sj.ki.5002553
更新日期：2007-12-01 00:00:00
abstract：BACKGROUND:The pathophysiology of hypertension in patients with chronic kidney disease (CKD) is largely attributed to positive sodium balance. It is unclear how loop diuretics affect fluid volume compartments, especially with respect to their antihypertensive effect. METHODS:Subjects with CKD were administered a singl...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1046/j.1523-1755.2003.00273.x
更新日期：2003-11-01 00:00:00
abstract：BACKGROUND:Antineutrophil cytoplasm antibodies (ANCAs) are implicated in the pathogenesis of systemic vasculitis. We asked whether ANCA could induce nitric oxide (NO) release from human neutrophils and, if so, whether this NO production was dependent on NO synthase (NOS) activity. METHODS:Neutrophil NO production was ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.059002593.x
更新日期：2001-02-01 00:00:00
abstract：:To evaluate the effect of continuous ambulatory peritoneal dialysis (CAPD) on the anemia of endstage renal disease, serial measurements of red cell mass and other hematological parameters were performed in 34 patients. Twenty-five patients were measured at the start and at 6 months (group 1), 13 at the start, at 6 and...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1983.88
更新日期：1983-05-01 00:00:00
abstract：BACKGROUND:The purpose of this study was to examine the relationship between glomerular filtration rate (GFR) measured at 5 years' diabetes duration and annual urine albumin excretion in a prospective cohort of children with type 1 diabetes (T1DM). METHODS:Three hundred and eight children were followed from diagnosis ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2005.00590.x
更新日期：2005-10-01 00:00:00
abstract：:The cytokine interleukin-6 (IL-6) is a major cell regulatory factor that may play an important role in the bone remodeling of patients with renal failure. IL-6 exerts its action by binding to its receptor (IL-6R), which leads to transduction of a second messenger cascade within cells. In vitro as well as in vivo data ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.343
更新日期：1996-08-01 00:00:00
abstract：:Sodium depletion has a protective effect on target-organ damage in hypertension independent of blood pressure. Here we tested whether chronic dietary sodium restriction may prevent the development of renal alterations associated with insulin resistance by reducing the inflammatory and oxidant state. Rats were fed norm...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2012.478
更新日期：2013-04-01 00:00:00
abstract：:The thick ascending limb of Henle's loop ( TAL ) develops a specific and consistent structural lesion during perfusion of the isolated rat kidney that progresses from mitochondrial swelling at 15 min to complete cellular disruption at 90 min. Because it seemed possible that damage localized to this metabolically activ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1984.9
更新日期：1984-01-01 00:00:00
abstract：:Chronic kidney disease is characterized as impaired renal function along with the imbalance and dysregulation of mineral metabolism; recognized as chronic kidney disease-mineral and bone disorder. Hyperphosphatemia, characterized by altered phosphate homeostasis along with elevated fibroblast growth factor-23 and inta...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2020.02.040
更新日期：2020-08-01 00:00:00
abstract：BACKGROUND:Urea kinetic modeling (UKM) and food records are widely used to assess the dialysis adequacy. Clinicians use these methods in individual patients to decide whether the dialysis prescription should be adjusted. We determined the variation in UKM parameters and dietary intake within individual patients in orde...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1046/j.1523-1755.1999.00412.x
更新日期：1999-05-01 00:00:00
abstract：:Intestinal regulation of oxalate absorption is a complex mechanism, not exclusively reliant on the oxalate-degrading anaerobe Oxalobacter formigenes. Using metagenomics, Miller et al. were able to describe a network of bacterial taxa co-occurring with Oxalobacter formigenes in fecal samples from non-stone forming cont...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2019.03.020
更新日期：2019-07-01 00:00:00
abstract：:Patients cared for in the intensive care unit (ICU) undergo multiple interventions to treat serious medical conditions. In addition to the acute illness being treated, underlying chronic conditions require ongoing drug therapy. As a result, these patients are exposed to numerous pharmaceutical agents, many of which ha...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2010.475
更新日期：2012-06-01 00:00:00
abstract：:Arguably, no cell biology related to the loss of renal function is more debated than the ontogeny of fibrosis in the kidney. Recent studies suggest that the fibroblasts depositing most of the excess extracellular matrix in diseased kidneys derive from epithelial cells versus pericytes resident within the kidney. Reich...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2013.107
更新日期：2013-07-01 00:00:00
abstract：:PDGF and TGF-beta are known mediators of mesangial cell proliferation and matrix expansion. The presence of these regulatory factors was examined in 30 renal biopsies from patients with IgA glomerulonephritis (IgA-GN) at the mRNA and protein level. Normal renal tissue served as control. The mRNA expression of PDGF A/B...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1995.443
更新日期：1995-11-01 00:00:00
