Abstract：
:Urinary exosomes have received considerable attention as a potential biomarker source for the diagnosis of renal diseases. Notwithstanding, their use in protein biomarker research is hampered by the lack of efficient methods for vesicle isolation, lysis, and protein quantification. Here we report an improved ultracentrifugation-based method that facilitates the solubilization and removal of major impurities associated with urinary exosomes. A double-cushion sucrose/D(2)O centrifugation step was used after a two-step differential centrifugation to separate exosomes from the heavier vesicles. After the removal of uromodulin, 378 and 79 unique proteins were identified, respectively, in low- and high-density fractions. Comparison of our data with two previously published data sets helped to define proteins commonly found in urinary exosomes. Lysis, protein extraction, and in-solution digestion of exosomes were then optimized for MudPIT application. More than a hundred exosomal proteins were quantified by four-plex iTRAQ analysis of single and pooled samples from two different age groups. For healthy men, six proteins (TSN1, PODXL, IDHC, PPAP, ACBP, and ANXA5) showed significant expression differences between exosome pools of those aged 25-50 and 50-70 years old. Thus, exosomes isolated by our method provide the basis for the development of robust quantitative methods for protein biomarker research.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Raj DA,Fiume I,Capasso G,Pocsfalvi Gdoi
10.1038/ki.2012.25subject
Has Abstractpub_date
2012-06-01 00:00:00pages
1263-72issue
12eissn
0085-2538issn
1523-1755pii
S0085-2538(15)55251-4journal_volume
81pub_type
杂志文章abstract：:Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a recently described member of the epidermal growth factor (EGF) family. It binds to heparan sulfate proteoglycans via a cationic domain and is a potent mitogen for epithelial cells, fibroblasts and vascular smooth muscle cells. In the present stud...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1998.00846.x
更新日期：1998-05-01 00:00:00
abstract：BACKGROUND:In addition to nitric oxide (NO) and prostacyclin (PGI2), the vascular endothelium can influence local vascular tone by a mechanism involving the hyperpolarization of vascular smooth muscle cells. This response is attributed to the release of an endothelium-derived hyperpolarizing factor (EDHF). The present ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00910.x
更新日期：2003-05-01 00:00:00
abstract：BACKGROUND:The rat renal proximal tubule cells contain a large amount of monoamine oxidase, which catalyzes the oxidative deamination of catecholamines such as dopamine (DA). The aim of this study is to investigate the potential role of hydrogen peroxide (H2O2) produced by monoamine oxidase (MAO) isoform on regulation ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.00468.x
更新日期：2001-01-01 00:00:00
abstract：:In order to study the effects of acute ureteral obstruction on glomerular hemodynamics, glomerular hydrostatic capillary pressure (PG), pressure in the first-order peritubular capillaries (EAP), and intratubular pressure (PT) were directly measured in superficial nephrons on Munich-Wistar rats by micropuncture with a ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1977.131
更新日期：1977-12-01 00:00:00
abstract：:In this retrospective study, we analyzed clinical laboratory, and pathologic variables to determine their value in predicting survival and survival free of renal failure for 170 consecutive patients with idiopathic renal vasculitis and glomerulonephritis evaluated during a 15 year period. Of the 170 patients, 108 had ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1989.312
更新日期：1989-12-01 00:00:00
abstract：:Atypical hemolytic uremic syndrome and thrombotic thrombocytopenic purpura have traditionally been considered separate entities. Defects in the regulation of the complement alternative pathway occur in atypical hemolytic uremic syndrome, and defects in the cleavage of von Willebrand factor (VWF)-multimers arise in thr...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2016.03.023
更新日期：2016-07-01 00:00:00
abstract：:Autologous and allogeneic bone marrow grafting both require cytoreductive therapy but only the allogeneic procedure requires immunosuppressive agents. Allogeneic bone marrow transplantation has been reported to be associated with a high incidence of both renal failure and veno-occlusive disease (VOD) of the liver, the...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1038/ki.1996.405
更新日期：1996-09-01 00:00:00
abstract：:Our findings indicate that hypoaldosteronism occurs commonly (23/31 patients) in hyperkalemic patients with chronic renal insufficiency and that the deficiency of aldosterone contributes to the pathogenesis of the hyperkalemia. In most patients (83%), hypoaldosteronism could be accounted for by deficient renal secreti...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1980.11
更新日期：1980-01-01 00:00:00
abstract：:Approximately 500 monogenic causes of chronic kidney disease (CKD) have been identified, mainly in pediatric populations. The frequency of monogenic causes among adults with CKD has been less extensively studied. To determine the likelihood of detecting monogenic causes of CKD in adults presenting to nephrology servic...
journal_title：Kidney international
pub_type： 杂志文章,多中心研究
doi：10.1016/j.kint.2018.10.031
更新日期：2019-04-01 00:00:00
abstract：:The role of peritoneal dialysis (PD) in renal replacement therapy (RRT) remains unclear. There are no controlled trials to provide hard evidence of its efficacy. Comparative studies with haemodialysis from different centres and countries have given conflicting results even when allowing for case mix. Data from the Uni...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1046/j.1523-1755.1998.00183.x
更新日期：1998-12-01 00:00:00
abstract：:In organ transplantation, ischemia/reperfusion (I/R) results in damage that may affect cell viability and lead to organ failure. I/R injury involves a complex cascade of events, including loss of energy, derangement of the ionic hemostasis, production of reactive oxygen species, and cell death. In this context, mitoch...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1111/j.1523-1755.2004.761_9.x
更新日期：2004-08-01 00:00:00
abstract：BACKGROUND:Cold ischemia and reperfusion during kidney transplantation are associated with release of free oxygen radicals and damage of renal tubular cells. Bioflavonoids may diminish cold storage-induced injury due to antioxidant and iron chelating activities. This study was designed to delineate the renoprotective m...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00774.x
更新日期：2003-02-01 00:00:00
abstract：:LECT2 amyloidosis is the latest systemic type of amyloidosis to be described. It was discovered in patients with nephrotic syndrome and renal failure and is characterized by amyloid deposition in glomeruli, renal vessels, and interstitium. Clinical and pathological features of earlier phases of this type of amyloidosi...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2010.18
更新日期：2010-05-01 00:00:00
abstract：:Neutrophil (PMN) recruitment into the peritoneum during acute bacterial peritonitis is an important part of the host defense barrier in CAPD patients. However, the subsequent phagocytosis of bacteria may also lead to PMN degranulation and the release of lysosomal enzymes. We determined the concentration of neutrophil ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1993.216
更新日期：1993-07-01 00:00:00
abstract：:Our genetic hypercalciuric (GH) rats have been selected and inbred for 29 generations to maximize urine calcium (UCa) excretion compared to identical gender controls (Ctl). To determine the effect of the increased UCa on urinary supersaturation and stone formation, we pair fed 15 GH and 15 Ctl rats a standard 1.2% cal...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1995.468
更新日期：1995-12-01 00:00:00
abstract：BACKGROUND:Mice overexpressing thymic stromal lymphopoietin (TSLP) develop mixed cryoglobulinemia with renal disease closely resembling human cryoglobulin-associated membranoproliferative glomerulonephritis (MPGN), including glomerular deposits of immunoglobulins and complement. We assessed the effect of complement inh...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2004.00495.x
更新日期：2004-04-01 00:00:00
abstract：:Patients cared for in the intensive care unit (ICU) undergo multiple interventions to treat serious medical conditions. In addition to the acute illness being treated, underlying chronic conditions require ongoing drug therapy. As a result, these patients are exposed to numerous pharmaceutical agents, many of which ha...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2010.475
更新日期：2012-06-01 00:00:00
abstract：:To examine whether hypoxia-inducible factor (HIF)-1α mediates the profibrotic effects of angiotensin II, we treated cultured renal medullary interstitial cells with angiotensin II and found that it increased HIF-1α levels. This was accompanied by a significant upregulation of collagen I/III, the tissue inhibitor of me...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2010.326
更新日期：2011-02-01 00:00:00
abstract：:A second generation hepatitis C virus recombinant immunoblot assay (RIBA) was used to screen stored perioperative serum from 641 renal allograft recipients. One hundred and nine (17%) were anti-HCV positive at the time of transplant. RIBA positivity was found to be an independent predictor of post-transplant liver dis...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1994.29
更新日期：1994-01-01 00:00:00
abstract：:Twelve patients with progressive renal failure were placed on a very low protein diet supplemented by an essential amino acid-keto acid mixture for six to twelve months. Total daily intake was 0.04 g nitrogen/kg and 50 kcal/kg. Eight subjects had a significant change in the slope of reciprocal plasma creatinine, becom...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1986.99
更新日期：1986-05-01 00:00:00
abstract：:One reason for the inadequacy of current renoprotective therapy and the persistent poor renal prognosis in diabetic nephropathy is the large interindividual variation in response to treatment. Genetic as well as non-genetic factors are known to influence treatment efficacy. This Commentary summarizes the impact of the...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/sj.ki.5000283
更新日期：2006-04-01 00:00:00
abstract：:Despite the importance of cardiovascular disease in dialysis patients, the frequency of atrial fibrillation in incident dialysis patients has not been determined. We analyzed the prevalence of atrial fibrillation in patients starting dialysis over a 4-year period, its occurrence over the course of dialysis, and its in...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2009.185
更新日期：2009-08-01 00:00:00
abstract：:Intradialytic hypotension is a most frequent complication of hemodialysis and may contribute to cardiovascular events and high mortality. There is a hypothesis that an increase in adenosine generation during hemodialysis may cause vasodilation and a decrease in cardiac output, which results in systemic hypotension. We...
journal_title：Kidney international
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1038/sj.ki.5000088
更新日期：2006-03-01 00:00:00
abstract：:Podocyte foot process effacement is characteristic of proteinuric renal diseases. In minimal change nephrotic syndrome (MCNS) foot processes are diffusely effaced whereas the extent of effacement varies in focal segmental glomerulosclerosis (FSGS). Here we measured foot process effacement in FSGS and compared it to th...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.413
更新日期：2008-12-01 00:00:00
abstract：BACKGROUND:Nitric oxide (NO) has been shown to be a paracrine/autocrine regulator of proximal tubular transport. In this study, we investigated the effect of NO on the paracellular permeability of opossum kidney (OK) cells, a proximal tubule cell line that possesses a leaky paracellular pathway resembling that of the i...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.00465.x
更新日期：1999-06-01 00:00:00
abstract：BACKGROUND:Fructose is a nutrient as well as a potent agent for the formation of advanced glycation end product in diabetes. GLUT5 is a facilitated-diffusion fructose transporter expressed in the small intestine and kidney. Previous reports on the localization of GLUT5 by in situ hybridization and immunohistochemistry ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.00635.x
更新日期：1999-09-01 00:00:00
abstract：:Suzuki et al. analyzed a mouse model of erythropoietin-deficient anemia to show that during iron overload renal interstitial fibroblasts accumulate iron, and this impairs the hypoxia-driven transcription of the erythropoietin gene. The authors show that excess iron decreases levels of hypoxia-inducible transcription f...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2018.07.027
更新日期：2018-11-01 00:00:00
abstract：:Recent studies suggest that postnatal neovascularization relies not exclusively on sprouting of preexisting vessels ("angiogenesis"), but also involves the contribution of bone marrow-derived circulating endothelial progenitor cells (EPCs). EPCs can be isolated from peripheral blood or bone marrow mononuclear cells, C...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1111/j.1523-1755.2005.00261.x
更新日期：2005-05-01 00:00:00
abstract：BACKGROUND:Glucose degradation products (GDPs) are cytotoxic in vitro and potentially toxic in vivo during peritoneal dialysis (PD). We are presenting the results of a two-year randomized clinical trial of a new PD fluid, produced in a two-compartment bag and designed to minimize heat-induced glucose degradation while ...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1046/j.1523-1755.2001.00497.x
更新日期：2001-01-01 00:00:00
abstract：:Disturbed pulsatile LH secretion has been suggested to play a role in the etiology of delayed puberty and disturbed reproductive function in chronic renal failure (CRF), but interpretation of gonadotropin secretion from plasma concentration measurements is confounded by alterations in hormone metabolic clearance. To s...
journal_title：Kidney international
pub_type： 杂志文章,多中心研究
doi：10.1038/ki.1994.191
更新日期：1994-05-01 00:00:00
