Expression of decay accelerating factor mRNA and complement C3 mRNA in human diseased kidney.


BACKGROUND:Decay accelerating factor (DAF), a product of mesangial cells in vitro, is expressed on the surface of cells and is a candidate for the focal suppression of complement activation. It is not clear at present whether the levels of expression of DAF and intrarenal C3 synthesis correlate with the level of tissue injury. METHODS:Immunohistochemistry for DAF and C3 and nonradioactive in situ hybridization with digoxigenin-labeled oligonucleotide probe for DAF and C3 mRNA were performed in 22 tissue samples of kidneys from patients with IgA nephropathy (IgAN), 6 with membranous nephropathy (MN), 6 with lupus nephritis (LN), and five normal kidneys. RESULTS:In the normal kidney, DAF was confined to the juxtaglomerular apparatus and little or no C3 was detected; however, a few glomerular cells were positive for DAF mRNA but no C3 mRNA positive cells were detected. In diseased kidneys, DAF and C3 as well as their mRNAs were detected in mesangial cells, tubular cells and infiltrating cells. Glomerular epithelial cells and Bowman's capsule cells contained little or no DAF and C3 but were positive for their mRNAs. The mean percentages of mesangial cells positive for DAF and C3 mRNAs were 49.3 +/- 11.5% and 50.7 +/- 10.3% in IgAN, and 17.0 +/- 6.3% and 19.4 +/- 9.0% in MN, respectively. The percentage of mesangial cells positive for DAF and C3 mRNAs among intraglomerular cells correlated positively with the degree of mesangial proliferation and glomerular sclerosis in IgAN. In contrast, in LN the percentage of glomerular cells positive for DAF mRNA correlated negatively with the degree of glomerular injury, while the percentage of cells positive for C3 mRNA did not change with the progression of the disease. The ratio of C3 mRNA/DAF mRNA of glomerular cells correlated with the degree of glomerular injury in both IgAN and LN. In the tubulointerstitium, the percentage of cells expressing mRNA, and C3 mRNA/DAF mRNA radio correlated with the degree of tubular atrophy and interstitial broadening in both IgAN and LN. CONCLUSIONS:We conclude that DAF and C3 mRNAs are synthesized in human diseased kidneys, and that a balance between locally synthesized DAF and C3 may be important in the progression of glomerulonephritis.


Kidney Int


Kidney international


Abe K,Miyazaki M,Koji T,Furusu A,Ozono Y,Harada T,Sakai H,Nakane PK,Kohno S




Has Abstract


1998-07-01 00:00:00














  • Outcomes following diagnosis of acute renal failure in U.S. veterans: focus on acute tubular necrosis.

    abstract::When patients develop acute kidney injury, a small fraction of them will develop end-stage renal disease later. The severity of renal impairment in the remaining patients is uncertain because studies have not carefully examined renal function over time or the precise timing of entry into a late stage of chronic kidney...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Amdur RL,Chawla LS,Amodeo S,Kimmel PL,Palant CE

    更新日期:2009-11-01 00:00:00

  • High glucose inhibits renal proximal tubule cell proliferation and involves PKC, oxidative stress, and TGF-beta 1.

    abstract:BACKGROUND:The alteration of renal cell growth is one of the early abnormalities in the diabetic nephropathy. However, the effects of high glucose and its action mechanism in renal proximal tubule cell (PTC) proliferation have not been elucidated. METHODS:The effects of 25 mmol/L glucose on cell proliferation, thymidi...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Park SH,Choi HJ,Lee JH,Woo CH,Kim JH,Han HJ

    更新日期:2001-05-01 00:00:00

  • Apoptosis in parathyroid hyperplasia of patients with primary or secondary uremic hyperparathyroidism.

    abstract:BACKGROUND:Chronic oversecretion of parathyroid hormone (PTH) is associated with parathyroid hyperplasia, reflecting a disturbed balance between cell proliferation and apoptosis. This study addressed the unsolved issue of apoptosis in hyperparathyroidism. METHODS:Parathyroid glands from 19 patients with primary (1 deg...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Zhang P,Duchambon P,Gogusev J,Nabarra B,Sarfati E,Bourdeau A,Drüeke TB

    更新日期:2000-02-01 00:00:00

  • The spectrum of bone disease in end-stage renal failure--an evolving disorder.

    abstract::We have assessed the bone histology in 259 chronic dialysis patients, all of whom were in the same dialysis program. All patients had bone biopsies with quantitative histomorphometry, intact parathyroid hormone (PTH) measurements, basal and deferoxamine stimulated serum aluminum levels. Results demonstrate the increas...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Sherrard DJ,Hercz G,Pei Y,Maloney NA,Greenwood C,Manuel A,Saiphoo C,Fenton SS,Segre GV

    更新日期:1993-02-01 00:00:00

  • CD14(++)CD16+ monocytes but not total monocyte numbers predict cardiovascular events in dialysis patients.

    abstract::Migration of monocytes into the vessel wall contributes to the onset and progression of atherosclerosis. Because monocytes are a heterogeneous population, we determined potential associations between monocyte subsets and cardiovascular events in a prospective cohort of 94 dialysis patients followed for 35 months. The ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Heine GH,Ulrich C,Seibert E,Seiler S,Marell J,Reichart B,Krause M,Schlitt A,Köhler H,Girndt M

    更新日期:2008-03-01 00:00:00

  • The role of urinary peptidomics in kidney disease research.

    abstract::Urinary peptidomics focuses on endogenous urinary peptide content. Many studies now show the usefulness of this approach for the discovery and validation of biomarkers in kidney diseases that are as varied as chronic kidney disease, acute kidney injury, congenital anomalies of the kidney and the urinary tract, and pol...

    journal_title:Kidney international

    pub_type: 杂志文章,评审


    authors: Klein J,Bascands JL,Mischak H,Schanstra JP

    更新日期:2016-03-01 00:00:00

  • Effect of angiotensin on the filtration of protein in the rat kidney: a micropuncture study.

    abstract::We have analyzed the protein content of proximal tubular fluid (PTF) by ultramicro disc electrophoresis and measured total protein excretion rates both in control conditions and during angiotensin infusion to the rat. Under control conditions PTF albumin concentration was 1.49 +/- 1.12 (SD) mg/100 ml and did not incre...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Eisenbach GM,Liew JB,Boylan JW,Manz N,Muir P

    更新日期:1975-08-01 00:00:00

  • The growth of acute kidney injury: a rising tide or just closer attention to detail?

    abstract::Acute kidney injury (AKI), previously termed acute renal failure, is associated with increased mortality, prolonged hospital stay, and accelerated chronic kidney disease (CKD). Over the past 2 decades, dramatic rises in the incidences of AKI have been reported, particularly within the United States. The question arise...

    journal_title:Kidney international

    pub_type: 杂志文章,评审


    authors: Siew ED,Davenport A

    更新日期:2015-01-01 00:00:00

  • Don't trick me twice!

    abstract::Chemotherapy-induced nephrotoxicity limits the success of cancer therapy. Landau et al. now describe a mechanism by which a first dose of cisplatin renders the kidney sensitive to necroptosis mediated by a second dose. Unresolved injury and sustained necrosis, therefore, may represent a pathophysiological means of tra...

    journal_title:Kidney international

    pub_type: 评论,杂志文章


    authors: Tonnus W,Belavgeni A,Xu Y,Linkermann A

    更新日期:2019-04-01 00:00:00

  • Changes in bone structure and the muscle-bone unit in children with chronic kidney disease.

    abstract::The impact of pediatric chronic kidney disease (CKD) on acquisition of volumetric bone mineral density (BMD) and cortical dimensions is lacking. To address this issue, we obtained tibia quantitative computed tomography scans from 103 patients aged 5-21 years with CKD (26 on dialysis) at baseline and 12 months later. G...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Tsampalieros A,Kalkwarf HJ,Wetzsteon RJ,Shults J,Zemel BS,Foster BJ,Foerster DL,Leonard MB

    更新日期:2013-03-01 00:00:00

  • Predominant effect of kidney disease on mortality in Pima Indians with or without type 2 diabetes.

    abstract:BACKGROUND:We examined the effect of kidney disease (KD) on mortality in nondiabetic and diabetic Pima Indians aged > or = 45 years old. METHODS:Deaths and person-years of follow-up were stratified in a time-dependent fashion into categories of (1) no proteinuria and normal serum creatinine (SCr); (2) proteinuria and ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Pavkov ME,Bennett PH,Sievers ML,Krakoff J,Williams DE,Knowler WC,Nelson RG

    更新日期:2005-09-01 00:00:00

  • Mortality effect of coronary calcification and phosphate binder choice in incident hemodialysis patients.

    abstract::The risk of death in hemodialysis patients treated with calcium-containing phosphate binders or sevelamer is not known. We assessed all-cause mortality in 127 patients new to hemodialysis assigned to calcium-containing binders or sevelamer after a median follow-up of 44 months from randomization. This was a predetermi...

    journal_title:Kidney international

    pub_type: 杂志文章,随机对照试验


    authors: Block GA,Raggi P,Bellasi A,Kooienga L,Spiegel DM

    更新日期:2007-03-01 00:00:00

  • Influence of initiation of maintenance hemodialysis on biomarkers of inflammation and oxidative stress.

    abstract:BACKGROUND:Chronic inflammation and oxidative stress are highly prevalent in patients with chronic kidney disease and end-stage renal disease (ESRD). These conditions contribute to high mortality rates associated with cardiovascular disease, the leading cause of death in this patient population. To our knowledge, no pr...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Pupim LB,Himmelfarb J,McMonagle E,Shyr Y,Ikizler TA

    更新日期:2004-06-01 00:00:00

  • Cisplatin-induced changes in sodium, chloride, and urea transport by the frog skin.

    abstract::Cisplatin is a platinum-containing antitumor agent whose usefulness is limited by nephrotoxicity. We examined the effects of cisplatin on a representative, transporting epithelium--the frog skin. Cisplatin (10(-3)M) from the mucosal surface increased the active transport of sodium by 35 to 40%, as monitored by short-c...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: van den Berg EK Jr,Brazy PC,Huang AT,Dennis VW

    更新日期:1981-01-01 00:00:00

  • Impact of uremia on female reproductive cyclicity, ovulation, and luteinizing hormone in the rat.

    abstract:BACKGROUND:Impaired reproductive function accompanies chronic renal insufficiency (uremia) in both the human and experimental animal. Clinical hypogonadism occurs in both genders. The present studies were designed to investigate possible anti-ovulatory effects of uremia in the female rat, a species that produces multip...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Krieg RJ,Tokieda K,Chan JC,Veldhuis JD

    更新日期:2000-08-01 00:00:00

  • Role of HSD11B2 polymorphisms in essential hypertension and the diuretic response to thiazides.

    abstract:BACKGROUND:The renal 11beta-hydroxysteroid dehydrogenase type 2 (11beta HSD2) enzyme inactivates 11-hydroxy steroids in the kidney, thereby protecting the nonselective mineralocorticoid (MR) receptor from occupation by glucocorticoids. Loss-of-function mutations in the gene encoding 11beta HSD2 (HSD11B2) result in over...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Williams TA,Mulatero P,Filigheddu F,Troffa C,Milan A,Argiolas G,Parpaglia PP,Veglio F,Glorioso N

    更新日期:2005-02-01 00:00:00

  • Regulatory innate lymphoid cells suppress innate immunity and reduce renal ischemia/reperfusion injury.

    abstract::Innate lymphoid cells are a recently recognized group of immune cells with critical roles in tissue homeostasis and inflammation. Regulatory innate lymphoid cells are a newly identified subset of innate lymphoid cells, which play a suppressive role in the innate immune response, favoring the resolution of intestinal i...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Cao Q,Wang R,Wang Y,Niu Z,Chen T,Wang C,Jin L,Huang Q,Li Q,Wang XM,Azmi F,Lee VWS,Wang YM,Zheng G,Alexander SI,Harris DCH

    更新日期:2020-01-01 00:00:00

  • Improvement of growth and food utilization by human recombinant growth hormone in uremia.

    abstract::We compared growth rate, food conversion ratio and morphology of the growth zone in female Sprague-Dawley rats with subtotal nephrectomy or sham operation. Both groups were either given vehicle or 1.4 IU/day recombinant human growth hormone (GH) by s.c. osmotic minipump, or 2.5 IU twice daily intraperitoneally for 14 ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Mehls O,Ritz E,Hunziker EB,Eggli P,Heinrich U,Zapf J

    更新日期:1988-01-01 00:00:00

  • Effects of oxygen breathing and erythropoietin on hypoxic vasodilation in uremic anemia.

    abstract::Loss of hypoxic vasodilation has been proposed as a causative factor in the development of hypertension in dialysis patients treated with recombinant human erythropoietin (rHuEPO). Venous occlusion plethysmography was therefore performed on 22 dialysis patients (aged 23 to 71 years, dialysis duration 6 to 260 months, ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Roger SD,Grasty MS,Baker LR,Raine AE

    更新日期:1992-10-01 00:00:00

  • Renal phosphate reabsorption in the rat: effect of inhibitors.

    abstract::The maximum rate of inorganic phosphorus (P1) reabsorption (Tmp1) was measured during phosphate infusion in chronically parathyroidectomized rats. In either initially hydropenic rats or others which underwent moderate preliminary saline loading, Tmp1 tended to rise during early phosphate infusion and then decline, so ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Engle JE,Steele TH

    更新日期:1975-08-01 00:00:00

  • Sources of human urinary epinephrine.

    abstract::The kidney is a likely source for some urinary epinephrine (E) since adrenalectomized animals and humans continue to excrete urinary E and the human kidney contains E synthesizing enzymes. We studied subjects during an intravenous infusion of 3H-E to determine the fraction of urinary E derived from the kidney. Eight n...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Ziegler MG,Aung M,Kennedy B

    更新日期:1997-01-01 00:00:00

  • Positive effect of the induction of p21WAF1/CIP1 on the course of ischemic acute renal failure.

    abstract:BACKGROUND:The p21 protein is found in the nucleus of most cells where it modulates cell cycle activity. At low levels, p21 stabilizes interactions between D cyclins and their cyclin-dependent kinases (cdks), but at high levels after induction by several different stress pathways, it causes cell cycle arrest. The p21 m...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Megyesi J,Andrade L,Vieira JM Jr,Safirstein RL,Price PM

    更新日期:2001-12-01 00:00:00

  • Improved assessment of bone turnover by the PTH-(1-84)/large C-PTH fragments ratio in ESRD patients.

    abstract:BACKGROUND:The "intact" parathyroid hormone (PTH) assay recognizes PTH-(1-84) as well as amino terminally truncated PTH fragments, that is, large carboxyterminal PTH fragments (C-PTH fragments). The present study investigated whether the use of the plasma PTH-(1-84)/C-PTH fragment ratio enhances the noninvasive assessm...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Monier-Faugere MC,Geng Z,Mawad H,Friedler RM,Gao P,Cantor TL,Malluche HH

    更新日期:2001-10-01 00:00:00

  • Genetic complexity in Joubert syndrome and related disorders.

    abstract::The recent identification of RPGRIP1L as a Joubert syndrome gene brings the total of known genes to five. Three of these are also associated with the lethal Meckel syndrome, and two with Senior-Løken syndrome; both of these disorders share Joubert syndrome phenotypes, illustrating the genetic complexity of this. ...

    journal_title:Kidney international

    pub_type: 杂志文章,评审


    authors: Harris PC

    更新日期:2007-12-01 00:00:00

  • Early and late adjustment to potassium loading in humans.

    abstract::We studied the adaptation to early (72 hr) and late (20 days) K loading (400 mmol/day, 4 equal meals every 6 hour) in six healthy humans. Throughout the study, each single K meal was followed by an acute transient rise in plasma K, aldosterone and kaliuresis. "K balance" (urinary K excretion approximately 80% of intak...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Rabelink TJ,Koomans HA,Hené RJ,Dorhout Mees EJ

    更新日期:1990-11-01 00:00:00

  • Preservation of residual kidney function in hemodialysis patients: reviving an old concept.

    abstract::Residual kidney function (RKF) may confer a variety of benefits to patients on maintenance dialysis. RKF provides continuous clearance of middle molecules and protein-bound solutes. Whereas the definition of RKF varies across studies, interdialytic urine volume may emerge as a pragmatic alternative to more cumbersome ...

    journal_title:Kidney international

    pub_type: 杂志文章,评审


    authors: Mathew AT,Fishbane S,Obi Y,Kalantar-Zadeh K

    更新日期:2016-08-01 00:00:00

  • Renal blood flow measurement by positron emission tomography using 15O-labeled water.

    abstract:BACKGROUND:Only few noninvasive methods have the potential to quantitate renal blood flow (RBF) in humans. Positron emission tomography (PET) is a clinical imaging method that can be used to measure the tissue blood flow noninvasively. The purpose of this study was to validate PET measurement of RBF using 15O-labeled w...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Juillard L,Janier MF,Fouque D,Lionnet M,Le Bars D,Cinotti L,Barthez P,Gharib C,Laville M

    更新日期:2000-06-01 00:00:00

  • Mechanisms of hypoalbuminemia in hemodialysis patients.

    abstract::Hypoalbuminemia is the most powerful predictor of mortality in end-stage renal disease. Since protein-calorie malnutrition can decrease albumin synthesis it is assumed that hypoalbuminemia results principally from malnutrition in these patients, but albumin synthesis may also be decreased as part of the acute-phase re...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Kaysen GA,Rathore V,Shearer GC,Depner TA

    更新日期:1995-08-01 00:00:00

  • The secretory response of parathyroid hormone to acute hypocalcemia in vivo is independent of parathyroid glandular sodium/potassium-ATPase activity.

    abstract::The involvement of sodium/potassium-ATPase in regulating parathyroid hormone (PTH) secretion is inferred from in vitro studies. Recently, the α-klotho-dependent rapid recruitment of this ATPase to the parathyroid cell plasma membrane in response to low extracellular calcium ion was suggested to be linked to increased ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Martuseviciene G,Hofman-Bang J,Clausen T,Olgaard K,Lewin E

    更新日期:2011-04-01 00:00:00

  • Effect of erythropoietin therapy and withdrawal on blood coagulation and fibrinolysis in hemodialysis patients.

    abstract::Erythropoietin (EPO) therapy in hemodialysis patients may be associated with an enhanced risk of vascular access and extracorporeal thrombosis. Assessment of blood coagulation and fibrinolysis was performed monthly on a group of 21 hemodialysis patients treated with EPO, and on four iron-deficient hemodialysis patient...

    journal_title:Kidney international

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Taylor JE,Belch JJ,McLaren M,Henderson IS,Stewart WK

    更新日期:1993-07-01 00:00:00