Abstract：
:Stable isotope labeling by amino acids in cell culture (SILAC) is a powerful approach for high-throughput quantitative proteomics. SILAC allows highly accurate protein quantitation through metabolic encoding of whole cell proteomes using stable isotope labeled amino acids. Since its introduction in 2002, SILAC has become increasingly popular. In this chapter we review the methodology and application of SILAC, with an emphasis on three research areas: dynamics of posttranslational modifications, protein-protein interactions, and protein turnover.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Hoedt E,Zhang G,Neubert TAdoi
10.1007/978-3-319-06068-2_5subject
Has Abstractpub_date
2014-01-01 00:00:00pages
93-106eissn
0065-2598issn
2214-8019journal_volume
806pub_type
杂志文章,评审abstract：:Legume proteins are relatively deficient in the sulfur amino acids. Among the different strategies to increase the sulfur amino acid contents of legumes, we have chosen to increase the biosynthesis of endogenous, non-abundant sulfur-rich proteins we have identified in soybean seed. We identified and isolated an 8 kDa ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4615-4729-7_10
更新日期：1999-01-01 00:00:00
abstract：:12-lipoxygenase (12-LOX) expression and function in the regulation of the metastatic phenotype was demonstrated in several murine melanoma lines before. Here we have provided novel evidences that, though at a low level (in max. 15% of the cell population), human melanoma lines (HT168, M1, HT199, HT18 and WM35) express...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-4793-8_89
更新日期：1999-01-01 00:00:00
abstract：:Midbrain dopaminergic neurons (mDA), comprising the substanti nigra pars compacta (A8), the ventral tegmental area (A9) and the retrorubal field (A10) subgroups, are generated from floor plate progenitors, rostral to the isthmic boundary. Floor plate progenitors are specified to become mDA progenitors between embryoni...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4419-0322-8_5
更新日期：2009-01-01 00:00:00
abstract：:Post-natal skeletal muscle is a highly plastic tissue that has the capacity to regenerate rapidly following injury, and to undergo significant modification in tissue mass (i.e. atrophy/hypertrophy) in response to global metabolic changes. These processes are reliant largely on soluble factors that directly modulate mu...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-27511-6_7
更新日期：2016-01-01 00:00:00
abstract：:Polymyxin B is another clinically available polymyxin that has re-emerged in clinical practice to treat infections caused by multi-drug (MDR) or extensively-drug-resistant (XDR) Gram-negative bacteria (GNB). Its chemical structure is very similar to the structure of polymyxin E (colistin). However, since the latter is...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-16373-0_14
更新日期：2019-01-01 00:00:00
abstract：:Cancer treatment has greatly improved over the last 50 years, but it remains challenging in several cases. Useful therapeutic targets are normally unique peculiarities of cancer cells that distinguish them from normal cells and that can be tackled with appropriate drugs. It is now known that cell metabolism is rewired...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-981-13-7709-9_9
更新日期：2019-01-01 00:00:00
abstract：:Asthma is a chronic inflammatory disease characterized by bronchospasms accompanied with frequent coughing, the pathogenesis of which is not clear. In healthy adults deep inspirations (DIs) provide a protective effect against bronchoconstriction triggered by methacholine inhalation, which correlates with the number of...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-94-007-4546-9_8
更新日期：2013-01-01 00:00:00
abstract：:Because several antigenic peptides of human tumors that are recognized by T-lymphocytes have been identified, immune responses against cancer can now be artificially manipulated. Furthermore, since T-lymphocytes have been found to play an important role in the rejection of tumors by the host and also to have antigen-s...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4614-3146-6_6
更新日期：2012-01-01 00:00:00
abstract：:The level of illness acceptance correlates positively with compliance to the doctor's recommendations, and negatively with the frequency and intensity of complications of chronic diseases. The purpose of this study was to determine the influence of the clinical condition on the level of illness acceptance, and to find...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/5584_2014_47
更新日期：2015-01-01 00:00:00
abstract：:The mechanisms of sensory transduction in the fine nerve terminals of free nerve endings supplied by Adelta and C sensory axons are largely a matter of speculation. This is because the nerve terminals are small and inaccessible, particularly in intact tissues like skin. However, some of the difficulties associated wit...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-0713-0_3
更新日期：2002-01-01 00:00:00
abstract：:In the second half of the 20th century it became obvious that a relentless increase in Type 2 diabetes mellitus (T2DM), affecting the economically affluent countries, is gradually afflicting also the developing world. This chapter shows the threat that the T2DM epidemic represents to mankind, with the astonishing rece...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4614-5441-0_6
更新日期：2012-01-01 00:00:00
abstract：:Biochemical imbalances, provoked by aging or a secondary illness, might directly affect the brain, causing severe problems, such as loss of memory or alteration of behavior patterns. Brain disorders are usually classified as injuries (such as stroke, hematomas, and concussions), tumors, and neurodegenerative (such as ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-90143-5_2
更新日期：2018-01-01 00:00:00
abstract：:Immunization of strain 2 guinea pigs with 10(7) syngeneic Ia+ L2C leukemia cells in adjuvant leads to L2C tumor protection. After subsequent challenges with L2C tumor cells, the sera and spleens of the protected guinea pigs had detectable anti-L2C reactivity. The L2C antibody reactivity was preferentially associated w...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4757-1410-4_7
更新日期：1983-01-01 00:00:00
abstract：:Subjective loudness measurements are used to create equal-loudness contours and auditory weighting functions for human noise-mitigation criteria; however, comparable direct measurements of subjective loudness with animal subjects are difficult to conduct. In this study, simple reaction time to pure tones was measured ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4939-2981-8_33
更新日期：2016-01-01 00:00:00
abstract：:Thermomechanical Analysis (TMA), using the parallel-plate-rheometer mode of the DuPont 943 TMA, was used to evaluate the softening properties of frozen aqueous carbohydrate model systems. A sample (of fixed dimension) of frozen solution was placed between the parallel plates and cooled to -80 degrees C. The sample was...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4899-0664-9_11
更新日期：1991-01-01 00:00:00
abstract：:Chronic nonmalignant pulmonary diseases impose a heavy burden on patients, generate health-care costs, and contribute to poor health-related quality of life. It has been found that a wide range of factors negatively affects quality of life, but the role of acceptance of illness needs to be further investigated. The ai...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/5584_2019_386
更新日期：2019-01-01 00:00:00
abstract：:Diabetes mellitus (DM) is a metabolic disorder characterised by hyperglycemia and abnormalities in carbohydrate, fat and protein metabolism. Several studies demonstrated that foods typical of the Mediterranean diet (MedDiet), including vegetables, fruits, oilseeds, extra virgin olive oil and fish, can promote health b...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/5584_2020_513
更新日期：2021-01-01 00:00:00
abstract：:R3230Ac mammary tumors were grown in transparent window chambers implanted into the dorsal skin flap of 250 g Fischer 344 rats (see Dewhirst et al, 1992). The oxygen pressure distributions in the tumor and host tissue were measured by the oxygen dependent quenching of phosphorescence (see Vinogradov et al, 1996) after...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-4863-8_71
更新日期：1998-01-01 00:00:00
abstract：:While studies of simple acoustic features have provided excellent bases for models of spatial hearing, we are seeking, here, to create a new paradigm for examination of shared attention and scene analysis in natural environments, where the listener is confronted with semantic information from multiple sources. In this...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4614-1590-9_58
更新日期：2013-01-01 00:00:00
abstract：:Inbred mice are routinely used as genetic models in lung biology. Among many phenotypic differences in lung function and structure, C3H/HeJ (C3) and C57BL/6J (B6) inbred mice also demonstrate a significantly different ventilatory pattern during acute hypoxic challenge. The present study rejects the hypothesis that a g...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/0-306-46825-5_8
更新日期：2000-01-01 00:00:00
abstract：:Enveloped viruses rely on fusion proteins in their envelope to fuse the viral membrane to the host-cell membrane. This key step in viral entry delivers the viral genome into the cytoplasm for replication. Although class II fusion proteins are genetically and structurally unrelated to class I fusion proteins, they use ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4614-7651-1_8
更新日期：2013-01-01 00:00:00
abstract：:Sarcopenia is recognized as a major health problem among older adults. This syndrome is associated with serious health consequences in terms of frailty, disability, morbidity and mortality. The aim of this study is to review sarcopenia and its impact on quality of life (QoL). MEDLINE database was searched from January...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-57379-3_19
更新日期：2017-01-01 00:00:00
abstract：:To identify a role for a Na:H (NHE) exchanger subtype in volume regulation in bovine corneal epithelium, we determined: 1. its sensitivity to inhibition by amiloride analogues 2. the effects of either Cl removal or hypertonicity on the intracellular pH. Our results suggest that volume regulatory responses elicited by ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-2417-5_18
更新日期：1994-01-01 00:00:00
abstract：:The presence and significance of tumor hypoxia has been recognized since the 1950's. Hypoxic cells in vitro and in animal tumors in vivo are documented to be three times more resistant to radiation-induced killing compared to aerobic cells. There is now evidence that tumor hypoxia is treatment-limiting in many human c...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-5865-1_26
更新日期：1997-01-01 00:00:00
abstract：:Programmed cell death occurs in the nervous system both in normal development as well as in pathologic conditions, and is a key issue related to both brain repair and neurodegenerative diseases. Modulation of cell death in the nervous system may involve neurotrophic factors and other peptides, neurotransmitters and ne...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/0-387-30128-3_10
更新日期：2006-01-01 00:00:00
abstract：:In this study, we investigated the hepatoprotective activity of the water extract derived from Octopus vulgaris meat (OM). First of all, a water extract prepared from OM (OMW) showed the high extraction yield (48.22%) and the highest taurine content (39.84%) in free amino acids. OMW exhibited the high value of reducin...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-981-13-8023-5_60
更新日期：2019-01-01 00:00:00
abstract：:A single protein is often capable of binding with many partners, enabling potential effects on either protein, such as modifying its expression or activity. However, due to the complex nature of in vivo systems, it is often difficult to perform nontargeted assays with a protein of interest. Methods in discovery proteo...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-3-319-52479-5_19
更新日期：2017-01-01 00:00:00
abstract：:Though soft tissue disorders have been recognized and described to some detail in several types of domestic animals and small mammals for some years, not much progress has been made in our understanding of the biochemical basis and pathogenesis of these diseases in animals. Ehlers-Danlos syndrome described in dogs alr...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-94-007-7893-1_14
更新日期：2014-01-01 00:00:00
abstract：:Maturation of the fetal lung is a complex process involving interactions between intrinsic biologic development of several cell types and hormonal triggering of biochemical and functional alterations. Catecholamines, corticosteroids, thyroid hormones and perhaps prolactin, all influence the transition from non-functio...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：
更新日期：1991-01-01 00:00:00
abstract：:Hemophagocytic lymphohistiocytosis (HLH) is a severe systemic syndrome associated with hyperactivation of macrophages and impaired regulation of the immune system. Two forms of HLH are currently recognized: genetically determined or familial (FHLH), and secondarily developed in the course of primary diseases, like aut...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/5584_2016_210
更新日期：2016-01-01 00:00:00
