Abstract:
:A single protein is often capable of binding with many partners, enabling potential effects on either protein, such as modifying its expression or activity. However, due to the complex nature of in vivo systems, it is often difficult to perform nontargeted assays with a protein of interest. Methods in discovery proteomics must be used to find potential interactors to pave the way for additional, more focused studies. This protocol describes the biological steps needed to create an interactome focused on a single protein target through co-immunoprecipitation.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Smith BJ,Cassoli JS,Guest PC,Martins-de-Souza Ddoi
10.1007/978-3-319-52479-5_19subject
Has Abstractpub_date
2017-01-01 00:00:00pages
229-236eissn
0065-2598issn
2214-8019journal_volume
974pub_type
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