Abstract:
:Parathion is a widely used organophosphoric pesticide which has also been reported to interfere with mouse spermatogenesis. Moreover it has been related to prenatal toxicity in mammals. Sixteen A/ Snell mice were sacrificed at day 17 of pregnancy. Testes and epididymides of the male fetuses were implanted in the allantochorion of chicken eggs. Three experimental conditions of the egg injections were considered: Group I: 1 ml of parathion (0.5 mg/ml), Group II: 1 ml of parathion (1 mg/ml), and Group III: 1 ml distilled water (control group). The implanted subjects continued their development for 4 days (i.e. to complete the gestational period for mice). The cell proliferation and differentiation of the epithelial cells of the epididymis were evaluated with the use of the monoclonal antiproliferating cell nuclear antigen (PCNA-cyclin) antibody, and the AE1 keratin complex antibody. Parathion altered the allantochorion, as 15% of the chicken embryos died in Group I and 40% in Group II, vs. only 8% in controls (Group III). However, no malformations were seen in the surviving embryos. In the testicular implants, the seminiferous cords of Group I had the same cytological characteristics of germ and pre-Sertoli cells as the control, except for involuting Leydig cells. Contrarily, in the cases with higher doses of parathion (Group II), there was a complete disorganisation of the seminiferous cords and the interstitium. In some testes, hyaline degeneration of the seminiferous cords was observed. No cell proliferation was evident, and the epididymal morphology was apparently unaffected. Therefore, parathion seems to interfere with normal testicular differentiation. However, in spite of interstitial damage, the epididymal development seems unaltered. Since the epididymis is an androgen-dependent organ, it may be postulated that testosterone production is still sufficient to support epididymal development but not spermatogenic cell line differentiation.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Rojas M,Bustos-Obregón E,Martínez-García F,Contreras H,Regadera Jdoi
10.1007/978-1-4899-0089-0_23subject
Has Abstractpub_date
1998-01-01 00:00:00pages
201-6eissn
0065-2598issn
2214-8019journal_volume
444pub_type
杂志文章abstract::Peripheral blood lymphocytes of 29 c-ANCA positive WG patients fulfilling the ACR classification criteria were examined for the expression of various leukocyte surface molecules by dual marker cytofluorometry (FACStar, Becton Dickinson). Activation markers such as CD25, HLA-DR, CD29 and adhesion molecules (ICAM-1 and ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4757-9182-2_69
更新日期:1993-01-01 00:00:00
abstract::Ventricular fibrillation is the major underlying cause of sudden cardiac death. Understanding the complex activation patterns that give rise to ventricular fibrillation requires high resolution mapping of localized activation. The use of multi-electrode mapping unraveled re-entrant activation patterns that underlie ve...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-17641-3_14
更新日期:2015-01-01 00:00:00
abstract::The virus-derived protein Apoptin has the ability to induce p53-independent apoptosis in a variety of human cancer cells while leaving normal cells unharmed. It thus represents a potential anti-cancer therapeutic agent of the future but a proper understanding of Apoptin-induced signalling events is necessary prior to ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4471-6458-6_2
更新日期:2014-01-01 00:00:00
abstract::In a mouse model of acute light-induced retinal degeneration, positive correlations between the levels of DHA, the levels of n3 PUFA lipid peroxidation, and the vulnerability to photooxidative stress were observed. On the other hand, higher sensitivity of the electroretinogram a-wave response, a measure of the amplifi...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4419-1399-9_65
更新日期:2010-01-01 00:00:00
abstract::The activity of liver macrophages was evaluated using a magnetometric method after administration of different perfluorochemicals. Following treatment with perfluoroctylbromide a significant shorter time period of diminished macrophage activity was found compared with a mixture of perfluorodecalin and perfluorotriprop...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-2468-7_28
更新日期:1994-01-01 00:00:00
abstract::The tremendous progress in engineering and computing power coupled with ultrasound transducer technology and imaging modalities over the past 20 years have encouraged a revival of clinical interest in ultrasound therapy, mainly in High-Intensity Focused Ultrasound (HIFU). So far, the most extensive results from HIFU o...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-22536-4_2
更新日期:2016-01-01 00:00:00
abstract::Several lines of evidence suggest that sensory nerves of the carotid body have an efferent function in addition to their afferent function of conducting chemoreceptive impulses to the brain. However, it has been difficult to document the release of substances from sensory nerve terminals on glomus cells and to determi...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:
更新日期:1996-01-01 00:00:00
abstract::We identified a 45 kDa Ca(2+)-dependent Lf binding protein on rat hepatocytes. Dithiobis(sulfosuccimidylproprionate) (DTSSP)-crosslinked 125I-Lf to a 45 kDa adduct in a Ca(2+)-dependent manner on intact cells. The 125I-labeled crosslinked complexes were absent when either surface-bound 125I-Lf was stripped prior to cr...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Skin is the main organ that covers the human body and acts as a protective barrier between the human body and the environment. Skin tissue as a stem cell source can be used for transplantation in therapeutic application in terms of its properties such as abundant, easy to access, high plasticity and high ability to re...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/5584_2019_380
更新日期:2020-01-01 00:00:00
abstract::Exposure to sunlight is a major source of vitamin D for most people. Yet public health advice has focused overwhelmingly on avoiding exposure of unprotected skin because of the risks of erythema and skin cancer. Given that there are also health risks associated with low vitamin D status, we explore the possibilities o...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-030-46227-7_20
更新日期:2020-01-01 00:00:00
abstract::1) Motility seems to be required for an intimate association with the intestinal mucosa which is necessary for efficient toxin delivery in the pathogenicity of V. cholerae. 2) A vaccine of semipurified flagella (CF) afforded a high degree of active and passive immunity in the rabbit ileum to both homologous and hetero...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4684-3369-2_50
更新日期:1978-01-01 00:00:00
abstract::The 3'-portion of the genome from a Taiwan isolate of porcine reproductive and respiratory syndrome (PRRS) virus, strain MD-001, was cloned and sequenced. The resultant 549 nucleotides contained an open reading frame with a coding capacity of 123 amino acids (predicted Mr 13,600). The predicted protein corresponds to ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-5331-1_103
更新日期:1998-01-01 00:00:00
abstract::Alterations in structural and biochemical properties of muscles that underlie physiological parameters of contractile force, speed and fatigability are described under conditions of 1) overuse: imposed electrical stimulation, natural exercise and functional overload; 2) reinnervation of denervated muscles; and 3) unde...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:
更新日期:1995-01-01 00:00:00
abstract::Erdosteine as a mucolytic agent that decreases mucus viscosity and facilitates mucus expulsion from the airways by cough or ciliary movement. Our objective was to determine whether erdosteine can directly contribute to mucus clearance. We addressed the issue by monitoring acute and chronic effects of erdosteine on cil...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/5584_2017_48
更新日期:2018-01-01 00:00:00
abstract::We analyzed CD95(Fas/APO-1) antigen expression on bone marrow blasts in 38 children with acute lymphoblastic leukemia (ALL) receiving a treatment in the Department of Leukaemias at the Cancer Research Center in 1987-1989 years (n = 22) and in 1994-1997 years (n = 16). CD95 antigen expression was studied by monoclonal ...
journal_title:Advances in experimental medicine and biology
pub_type: 临床试验,杂志文章
doi:10.1007/978-1-4615-4811-9_27
更新日期:1999-01-01 00:00:00
abstract::Programmed cell death occurs in the nervous system both in normal development as well as in pathologic conditions, and is a key issue related to both brain repair and neurodegenerative diseases. Modulation of cell death in the nervous system may involve neurotrophic factors and other peptides, neurotransmitters and ne...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/0-387-30128-3_10
更新日期:2006-01-01 00:00:00
abstract::Although a time-resolved near-infrared spectroscopy (TRS) system is difficult to make a measurement into 10 s or less at the moment, the system has a great advantage that it measures absolute values of hemoglobin concentrations. In the present study, using a device equipped with a TRS system, we examined individual di...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4419-1241-1_36
更新日期:2010-01-01 00:00:00
abstract::Although prevalence estimates are imprecise, growing numbers of women in the United States are living longer with metastatic breast cancer, attributable at least in part to the availability of effective targeted therapies. Women living with metastatic disease are understudied, however, and substantial heterogeneity ex...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-319-16366-6_16
更新日期:2015-01-01 00:00:00
abstract::Hematological malignancies (HM) are a heterogeneous group of life-threatening hematological diseases. The heterogeneity and clonal evolution of HM subpopulations are the main obstacles for precise diagnoses, risk stratification, and even targeted therapies. Standard bulk-sample genomic examinations average total mutat...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-981-13-0502-3_10
更新日期:2018-01-01 00:00:00
abstract::As a critical post-translational modification, phosphorylation plays important roles in regulating various biological processes, while recent studies suggest that phosphorylation in bacteria is also critical for functional signaling transduction. Since identification of phosphorylation substrates and sites is fundamen...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-94-017-9245-5_16
更新日期:2015-01-01 00:00:00
abstract::Viruses and their protein capsids can be regarded as biologically evolved nanomachines able to perform multiple, complex biological functions through coordinated mechano-chemical actions during the infectious cycle. The advent of nanoscience and nanotechnology has opened up, in the last 10 years or so, a vast number o...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-39196-0_5
更新日期:2016-01-01 00:00:00
abstract::We address here aspects of the implementation of a memory evolutive system (MES), based on the model proposed by A. Ehresmann and J. Vanbremeersch (2007), by means of a simulated network of spiking neurons with time dependent plasticity. We point out the advantages and challenges of applying category theory for the re...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-0-387-79100-5_2
更新日期:2010-01-01 00:00:00
abstract::The major central part of elastic ligaments is constituted by longitudinally orientated elastic fibres, each surrounded by a sheath of spirally orientated collagen fibrils with an intervening thin layer of microfibrils. In the terminal region of such ligaments the orientation of the collagen fibrils is longitudinal an...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4684-9093-0_9
更新日期:1977-01-01 00:00:00
abstract::Both hepatic lipogenesis from glucose and gluconeogenesis from lactate are inhibited by substrates for mixed function oxidation such as aminopyrine. These effects are inducible with phenobarbital pretreatment and absent with a demethylated product of aminopyrine metabolism, aminophenylpyrazolone. The data indicate tha...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4615-9026-2_25
更新日期:1975-01-01 00:00:00
abstract::Extensive use of antibiotics in both public health and animal husbandry has resulted in rapid emergence of antibiotic resistance in almost all human pathogens, including biothreat pathogens. Antibiotic resistance has thus become a major concern for both public health and national security. We developed multiplexed ass...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4419-5913-3_51
更新日期:2010-01-01 00:00:00
abstract::Calcium (Ca2+) buffering is part of an integrative crosstalk between different mechanisms and elements involved in the control of free Ca2+ ions persistence in the cytoplasm and hence, in the Ca2+-dependence of many intracellular processes. Alterations of Ca2+ homeostasis and signaling from systemic to subcellular lev...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-030-12457-1_7
更新日期:2020-01-01 00:00:00
abstract::This study was designed to assess risk for retinal toxicity associated with administration of high-dose sildenafil citrate to dogs heterozygous for a functionally null mutation in Pde6a over a 4-month period. Three Pde6a +/- dogs were administered 14.3 mg/kg sildenafil per os and two Pde6a +/- dogs placebo once daily ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-030-27378-1_61
更新日期:2019-01-01 00:00:00
abstract::In this brief review, I focused on the abdominal expiratory motor pattern in the rat. In the vagotomized adult rat, hypercapnic acidosis evoked two patterns of the abdominal expiratory activity; one with low amplitude expiratory discharge (E-all activity) that persisted throughout the expiratory phase, and another wit...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4419-5692-7_31
更新日期:2010-01-01 00:00:00
abstract::The survival of three-dimensional tissue requires a vascular network to provide transport of oxygen and metabolic byproduct. Here, we report a new approach to create capillary blood vessels in vitro on biomaterials suitable for use as scaffolds in engineering tissues. Endothelial cells were cultured on chemical and to...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-0-387-74911-2_23
更新日期:2008-01-01 00:00:00
abstract::In order to clarify the significance of the renal kallikrein(KAL)-kinin(KIN) system and prostaglandins (PG) in exaggerated natriuresis in essential hypertensives, the effect of acute sodium load on urinary KAL, KIN, PG, and renal water and sodium handling were investigated in normotensives (NT) and patients with essen...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4757-0154-8_42
更新日期:1986-01-01 00:00:00