Abstract：
:This study was designed to assess risk for retinal toxicity associated with administration of high-dose sildenafil citrate to dogs heterozygous for a functionally null mutation in Pde6a over a 4-month period. Three Pde6a +/- dogs were administered 14.3 mg/kg sildenafil per os and two Pde6a +/- dogs placebo once daily for 16 weeks. Three Pde6a +/+ dogs were administered sildenafil for 7 days. Ophthalmic examination, vision testing, and electroretinography (ERG) were regularly performed. At study termination, dogs were euthanized and globes collected. Retinal layer thickness and photoreceptor nuclei counts were determined from plastic sections. In both Pde6a +/- and Pde6a +/+ sildenafil-treated (ST) dogs, elevation of dark-adapted b-wave threshold and unmasking of the scotopic threshold response (STR) were observed. Sildenafil treated Pde6a +/- dogs had significantly thinner ONL (24.90 +/-1.88 μm, p = 0.004) and lower photoreceptor nuclei counts (273.6 +/- 29.3 cells/100 μm, p = 0.008) compared to measurements (35.90 +/- 1.63 μm) and counts (391.5 +/-27.0 cells/100 μm) from archived untreated Pde6a +/- dogs.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Pierce KE,Curran PG,Zelinka CP,Fischer AJ,Petersen-Jones SM,Bartoe JTdoi
10.1007/978-3-030-27378-1_61subject
Has Abstractpub_date
2019-01-01 00:00:00pages
371-376eissn
0065-2598issn
2214-8019journal_volume
1185pub_type
杂志文章abstract：:In hospitals, human milk is subjected to heat treatment to reduce risk of transmission of infectious agents such as human immunodeficiency virus (HIV), hepatitis B, cytomegalovirus, and bacterial contamination, especially during feeding of banked milk to preterm infants. Fat losses due to heat treatment have been exte...
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