Abstract：
:The past few decades have seen tremendous advances in single-particle electron -cryo-microscopy (cryo-EM). The field has matured to the point that near-atomic resolution density maps can be generated for icosahedral viruses without the need for crystallization. In parallel, substantial progress has been made in determining the structures of nonicosahedrally arranged proteins in viruses by employing either single-particle cryo-EM or cryo-electron tomography (cryo-ET). Implicit in this course have been the availability of a new generation of electron cryo-microscopes and the development of the computational tools that are essential for generating these maps and models. This methodology has enabled structural biologists to analyze structures in increasing detail for virus particles that are in different morphogenetic states. Furthermore, electron imaging of frozen, hydrated cells, in the process of being infected by viruses, has also opened up a new avenue for studying virus structures "in situ". Here we present the common techniques used to acquire and process cryo-EM and cryo-ET data and discuss their implications for structural virology both now and in the future.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Chang J,Liu X,Rochat RH,Baker ML,Chiu Wdoi
10.1007/978-1-4614-0980-9_4subject
Has Abstractpub_date
2012-01-01 00:00:00pages
49-90eissn
0065-2598issn
2214-8019journal_volume
726pub_type
杂志文章,评审abstract：:Cell and DNA replicative potentials were studied in 10 strains of skin fibroblasts from unrelated patients with the Werner syndrome (WS) and in a progeric CRL 1277 strain. The lifespans of all WS strains and of CRL 1277 cells are greatly abbreviated in vitro, due to large fractions of non-cycling cells and the basic p...
journal_title：Advances in experimental medicine and biology
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更新日期：2003-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-94-007-2888-2_45
更新日期：2012-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2013-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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更新日期：1998-01-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1007/978-3-319-67577-0_10
更新日期：2017-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-4-431-56550-5_20
更新日期：2017-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4614-4756-6_36
更新日期：2013-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：
更新日期：2012-01-01 00:00:00
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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更新日期：2008-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
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更新日期：2002-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2017-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1979-01-01 00:00:00
abstract：:Although a time-resolved near-infrared spectroscopy (TRS) system is difficult to make a measurement into 10 s or less at the moment, the system has a great advantage that it measures absolute values of hemoglobin concentrations. In the present study, using a device equipped with a TRS system, we examined individual di...
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pub_type： 杂志文章
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更新日期：2010-01-01 00:00:00
abstract：:Lipoproteins play a key role in regulating plasma and tissue levels of cholesterol. Apolipoprotein B (apoB)-containing lipoproteins, including chylomicrons, very-low density lipoprotein (VLDL) and low-density lipoprotein (LDL), serve as carriers of triglycerides and cholesterol and deliver these metabolites to periphe...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：2017-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
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更新日期：2016-01-01 00:00:00
